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Diversity and Phylogenetic Analysis of Culturable Marine Bacteria Isolated from Rhizosphere Soils of Suaeda japonica Makino in Suncheon Bay (순천만 칠면초의 근권으로부터 분리된 해양세균의 다양성 및 계통학적 분석)

  • You, Young-Hyun;Park, Jong Myong;Nam, Yoon-Jong;Kim, Hyun;Lee, Myung-Chul;Kim, Jong-Guk
    • Journal of Life Science
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    • v.25 no.2
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    • pp.189-196
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    • 2015
  • Bacterial diversity was studied in the rhizosphere of Suaeda japonica Makino, which is native to Suncheon Bay in South Korea. Soil samples from several sites were diluted serially, and pure isolation was performed by subculture using marine agar and tryptic soy agar media. Genomic DNA was extracted from 29 pure, isolated bacterial strains, after which their 16S rDNA sequences were amplified and analyzed. Phylogenetic analysis was performed to confirm their genetic relationship. The 29 bacterial strains were classified into five groups: phylum Firmicutes (44.8%), Gamma proteobacteria group (27.6%), Alpha proteobacteria group (10.3%), phylum Bacteriodetes (10.3%), and phylum Actinobacteria (6.8%). The most widely distributed genera were Bacillus (phylum Firmicutes), and Marinobacterium, Halomonas, and Vibrio (Gamma proteobacteria group). To confirm the bacterial diversity in rhizospheres of S. japonica, the diversity index was used at the genus level. The results show that bacterial diversity differed at each of the sampling sites. These 29 bacterial strains are thought to play a major role in material cycling at Suncheon Bay, in overcoming the sea/mud flat-specific environmental stress. Furthermore, some strains are assumed to be involved in a positive interaction with the halophyte S. japonica, as rhizospheric flora, with induction of growth promotion and plant defense mechanism.

Cultures of Ginkgo biloba, Effect of Nutritional and Hormonal Factors on the Growth of Cultured Cells Derived from Ginkgo biloba

  • Jeon, Mee-Hee;Sung, Sang-Hyun;Jeon, Soon-wha;Huh, Hoon;Kim, Jin-woong;Kim, Young-Choong
    • Archives of Pharmacal Research
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    • v.16 no.3
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    • pp.244-250
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    • 1993
  • Calli and suspension cultures were obtained following inoculation of the explant from leaves of Ginkgo biloba L on the supplemented MS basal medium. The obtained calli and suspension cultured cells were able to produce detectable amounts of ginkgolides which are known as natural specific PAF antagonists. The production of ginkgolides in the calli and suspension cultured celles were identified using GC/MS, GC and HPLC with authentic ocmpounds. Since the production of ginkgolides A and B the calli and suspension cultured cells had been confirmed, effects of types and concentration of plant growth regulators, media and illumination on the induction and growth of the callus were studied. The concentrations of growth regulators for optimal callus were studied. The concentrations of growth regulators for optimal callus induction were studied. The concentrations of growth regulators for optimal callus induction were 1.0 to 2.0 mg/L for NAA and o.1 mg/L for kinetin. The growth of the Callus seemed to be more simnultaed with the combination of NAA and kinetin than NAA and BA with illumination at all concentration ranges of 1.0 to 4.0 mg/l for NAA and o.1 to 1.0 mg/L for kinetin or BA studied. Amogn 8 different media used, the induction rate of callus on Anderson, Eriksson, and Shenk and Hildebrant at 4 weeks after the innoculation was almost the same as that of MS. However, callus was rarely induced on Heller or White medium. Suspension cultures were easily initiated with 3 g of callus (fresh weight) derived from ginkgo leaves on supplemented MS medium. A typical growth curve of suspension cultured cells could be obtained by measuring the fresh weight of the suspension cultured cells at every 3 days. To improve the growth of suspension cultured cells of ginkgo, effects of concentrations of NAA, sucrose, phosphate ions and molar ratio of $NH_{4}^+\;to\;NO_{3}^-$ ions in the culture medium were studied. The maximum growth of the cells was achieved when the culture medium contained 1.0 mg/L of NAA, 30 g/L sucrose, 1.75 mM phosphate ions and 1:5 molar ratio of $NH_{4}\;to\;NO_{3}^-$ ions.

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Feeding Behavior in the Plant Tissues with Green Peach Aphid (Myzus persicae, Aphididae; Homoptera) Using EPG Technique (EPG를 이용한 복숭아혹진딧물 (Myzus persicae, Aphididae, Homoptera)의 기주 식물체별 조직내 섭식행동)

  • Seo, M.J.;Jang, J.K.;Kang, E.J.;Kang, M.K.;Kim, N.S.;Yu, Y.M.;Youn, Y.N.
    • Korean journal of applied entomology
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    • v.44 no.4 s.141
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    • pp.271-276
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    • 2005
  • To investigate feeding behaviour of the greenpeach aphid (Myzus persicae) on several plants, DC electrical penetration graph (EPG) technique was used. We chose 5 plants including pepper, melon, cabbage, radish, and eggplant which were known as major host Plants of this species. This study was focused whether feeding patterns of the aphid were different and which plants would be the most preferable among 5 host plants. The time from initial proboscis contact with a each leaf until the first electrical contact, as a measure of the time taken for the stylet penetration, the time from electrical contact to the first potential drop as a time consumed until intracellular sampling, the number of potential drops per an hour during periods of regular intercellular pathway probing, and the time from electrical contact to tile first phloem specific pattern indicating the time taken to reach and attempt to feed upon the phloem were analysed. As a result, except the number of potential drop, there was no significant differency of feeding patterns among 5 plants. However, the feeding patterns related on host acceptability were observed more frequently from Pepper, radish, and e99r1an1 than melon and cabbage.

Study on Characteristics of Fine Bottom Ash Based Geopolymer Mortar (미분쇄 바텀애시 기반 지오폴리머 모르타르 특성에 관한 연구)

  • Lim, Gwi-Hwan;Lee, Jeong-Bae;Jeong, Hyun-Kyu;Kim, Seong-Soo
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.4 no.4
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    • pp.418-424
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    • 2016
  • This study is an experimental study on the recycling of bottom ash in coal ash discharged from a thermal power plant. Bottom ash has limited research on recycling because it has more porous and higher water absorption ratio than fly ash. In this paper, the bottom ash was pulverized to a specific surface area of $4,000cm^2/g$ in order to use as a binder, and the flow, compressive strength test and microstructure analysis of the bottom ash based geopolymer mortar were performed. The flow measurement results of the geopolymer mortar showed that the flow rate was improved by increasing mixing water as the molar concentration of activator was increased. Compressive strength increased with increasing curing temperature and molar concentration. Through the microstructure analysis, we could confirm the geopolymer gel produced by the reaction of the condensation polymerization. It is considered that it is possible to make the bottom ash based geopolymer concrete through proper molar concentration of activator and high temperature curing.

Development of Stand-Alone Risk Assessment Software for Optimized Maintenance Planning of Power Plant Facilities (발전설비 최적 정비를 위한 독립형 위험도 평가 소프트웨어 개발)

  • Choi, Woo Sung;Song, Gee Wook;Kim, Bum Shin;Chang, Sung Ho;Lee, Sang Min
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.39 no.11
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    • pp.1169-1174
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    • 2015
  • Risk-Risk-based inspection (RBI) has been developed in order to identify risky equipments that can cause major accidents or damages in large-scale plants. This assessment evaluates the equipment's risk, categorizes their priorities based on risk level, and then determines the urgency of their maintenance or allocates maintenance resources. An earlier version of the risk-based assessment software is already installed within the equipment management system; however, the assessment is based on examination by an inspector, and the results can be influenced by his subjective judgment, rather than assessment being based on failure probability. Moreover, the system is housed within a server, which limits the inspector's work space and time, and such a system can be used only on site. In this paper, the development of independent risk-based assessment software is introduced; this software calculates the failure probability by an analytical method, and analyzes the field inspection results, as well as inspection effectiveness. It can also operate on site, since it can be installed on an independent platform, and has the ability to generate an I/O function for the field inspection results regarding the period for an optimum maintenance cycle. This program will provide useful information not only to the field users who are participating in maintenance, but also to the engineers who need to decide whether to extend the lifecycle of the power machinery or replace only specific components.

Community Structure Analysis of Carpinus laxiflora Communities in Seoul (서울지역 서어나무림의 군집구조 분석)

  • Park, Byung-Chang;Oh, Choong-Hyeon;Cho, Chi-Woung
    • Korean Journal of Environment and Ecology
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    • v.23 no.4
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    • pp.333-345
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    • 2009
  • Carpinus laxiflora communities are known as the climax forest community of the temperate zone of Korea. There are three Carpinus laxiflora communities in Seoul. The purpose of this research is to analyze the characteristics of Carpinus laxiflora communities of Seoul and supply basic data for establishing of a management plan. The research sites are Sahmyook University, Mountain Dobong and Jinkwandong. This study considers investigation and the analysis of communities structure, soil characteristics and the growth increment of trees. As the result of study, we could know that the growth increment of the Carpinus laxiflora trees of Seoul is decreasing recently because of soil acidification and so on. Also the vegetation disturbance is increasing because of naturalized plant. Oak wilt disease has appeared in the oak forest around Sahmyook University that is the largest Carpinus laxiflora communities area in Seoul. For that reason the open gap was appeared in forest, and the spread of naturalized plants has been increasing in the gap. Furthermore, the Carpinus laxiflora trees are affected by the Platypus koryoensis which is a kind of oak wilt disease. And so, Unless there is a specific management plan, the survival of Carpinus laxiflora communities are uncertain in Seoul. Therefore the management plan of Carpinus laxiflora communities is necessary such as precise investigation, annual monitoring, etc. For the conservation of the Carpinus laxiflora communities, it is necessary to establish the management plan of the biodiversity for Seoul area.

Duration of Competition and the Competitive Ability of Red Rice with Rich by Replacement Diagram (적미(赤米)의 경합기간(競合期間)과 밀도(密度)가 수도생육(水稻生育) 및 수량(收量)에 미치는 영향(影響))

  • Ree, D.W.;Kim, J.C.;Kim, Y.H.;Hong, Y.K.;Son, S.Y.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.45-51
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    • 1987
  • This experiment was conducted to find out the critical competitive period and competitive ability of rice against red rice. Plant height and number of tillers of rice decreased as red rice competed with rice during active tillering stage. The maximum tillering stage of rice competed with red rice became about 10days earlier than those non-competed. Significant yield reduction of rice was observed in the plots competed for 20 days after transplating or longer. Such a yield reduction can be mainly attributed to the decrease in number of panicles per hill and grain number per panicle. In a replacement diagram for rice and red rice, their competition is turned out being antagonized. The relative yield total was considered lower than 1. Grain number, culm and panicle length of rice was not affected significantly by inter-specific competition. Rice grain yield reduction was highly correlated with the number of panicles of red rice. Three hundred panicles/$m^2$ of the red rice were necessary to reduce rice grain yield by 50% of the check plot.

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Tunneling in Severe Groundwater Inflow Condition (지하수 과다유입 조건하에서의 터널굴착)

  • Lee, Young-Nam;Kim, Dae-Young
    • Journal of the Korean GEO-environmental Society
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    • v.7 no.2
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    • pp.67-76
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    • 2006
  • For a hydro power plant project, the headrace tunnel having a finished diameter of 3.3 m was constructed in volcanic rocks with well-developed vertical joint and high groundwater table. The intake facility was located 20.3km upstream of the powerhouse and headrace tunnel of 20km in length and penstock of 440m in height connected the intake and the powerhouse. The typical caldera lake, Lake Toba set the geology at the site the caving of the ground caused tension cracks in the vertical direction to be developed and initial stresses at the ground to be released. High groundwater table(the maximum head of 20bar) in the area of well-connected vertical joints delayed the progress of tunnel excavation severely due to the excessive inflow of groundwater. The excavation of tunnel was made using open-shield type TBM and mucking cars on the rail. High volume of water inflowraised the water level inside tunnel to 70cm, 17% of tunnel diameter (3.9m) and hindered the mucking of spoil under water. To improve the productivity, several adjustments such as modification of TBM and mucking cars and increase in the number of submersible pumps were made forthe excavation of severe water inflow zone. Since the ground condition encountered during excavation turned out to be much worse, it was decided to adopt PC segment lining instead of RC lining. Besides, depending on the conditions of the water inflow, rock mass condition and internal water pressure, one of the invert PC segment lining with in-situ RC lining, RC lining and steel lining was applied to meet the site specific condition. With the adoption of PC segment lining, modification of TBM and other improvement, the excavation of the tunnel under severe groundwater condition was successfully completed.

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The differences in Constellation drawings among different countries

  • Karimova, Ulkar;Yi, Yu;Oh, Suyeon
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.95-95
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    • 2012
  • Constellations are formed of bright stars which appear close to each other on the sky, but are really far apart in space. The shapes you see all depend on your point of view. Back before people had televisions and electricity to light their homes at night, they spent a lot more time looking at the stars. People all over the world used their imaginations to draw pictures in the sky, as if it were a giant connect-the-dot game. The patterns they imagined are called constellations. People usually saw patterns that reflected their different cultures. Native Americans in North America imagined many animals and shapes from the natural world. The ancient Greeks found images of gods and goddesses in the stars. Sometimes people from very different parts of the world even imagined the same animal or shape in the same stars. Most of the constellations we recognize today were made up by the ancient Greeks around 6,000 years ago. Different constellations are visible at different times of year, so the first appearance of these patterns told farmers of the changing seasons and reminded them to plant or harvest their crops. The constellations also help us to find our way around the night sky and to remember which stars are which. The star names we use today are mostly from Greek and Arabic, but many are changed a bit from the original, as often happens when words are passed from one language to another. It can be difficult to picture just what those folks long ago were seeing in the stars, so don't be discouraged if you have trouble seeing their patterns. You can even make up your own! In ancient world all the countries or regions had their own way to name things up in the sky, make up stories and draw different shapes for constellations. Today there are 88 official constellations, but you may find that different books show their stars connected in slightly different ways. The official constellations are specific regions of the sky, so the exact patterns are not all that important. However in various cultures there are some famous star patterns that use stars from only apart of a constellation, or even connect stars from different constellations. These patterns of stars that are not official constellations are called asterisms. The Big Dipper is a very famous asterism, found in the constellation Ursa Major, or Great Bear.

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A Case Study of Mine Environmental Restoration using Coal Ash (발전회를 이용한 광산환경 복원사례 연구)

  • Yoo, Jong-Chan;Ji, Sang-Woo;Ahn, Ji-Whan;Kim, Chun-Sik;Shin, Hee-Young
    • Resources Recycling
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    • v.26 no.2
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    • pp.80-88
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    • 2017
  • Globally, there has been a lot of research related to recycling coal ash from power plant stations. This research is happening because there is a considerable shortage of sites for reclamation of increased coal ash every year. In addition, a variety of environmental pollutants have appeared because of mining activity. Abandoned coal mine, pits, and mine tailing piles caused pollutants to come to the surface resulting in serious damage for humans and the environment. Therefore in this study, we investigated whether or not coal ashes have the ability to prevent several environmental problems by mining in Korea and a manageable form recycling coal ashes. In overseas countries, there is a sufficient field of applicable cases where coal ash is used for neutralizing AMD (Acid Mine Drainage), covering of the waste materials, grouting, and soil amendments. However in Korea, since the coal ash is classified as a 'waste', there is an insufficient field applicable cases so far. Therefore it is necessary to establish a specific standard and management system for the utilization of coal ash based on the relevant precedent cases applied abroad in order to prevent environmental pollution caused by mining activity in Korea.