• International Journal of Oral Biology
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• 제40권4호
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• pp.183-187
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• 2015

The present study was aimed to evaluate the influence of glutaraldehyde (GA) concentration on multiple electron microscopic (EM) immunostaining using pre-embedding peroxidase and post-embedding immunogold method. Influence of various concentrations of GA included in the fixative on immuoreactivity was assessed in the multiple immunostaining using antisera against anti-transient receptor potential vanilloid 1 (TRPV1) for peroxidase staining and anti-GABA for immunogold labeling in the rat trigeminal caudal nucleus. Anti-TRPV1 antiserum had specificity in pre-embedding peroxidase staining when tissues were fixed with fixative containing paraformaldehyde (PFA) alone. Immunoreactivity for TRPV1 was specific in tissues fixed with fixative containing 0.5% GA at both perfusion and postfixation steps, though the immunoreactivity was weaker than in tissues fixed with fixative containing PFA alone. Tissues fixed with fixative containing 0.5% GA at the perfusion and postfixation steps showed specific immunogold staining for GABA. The results of the present study indicate that GA concentration is critical for immunoreactivity to antigens such as TRPV1 and GABA. This study also suggests that the appropriate GA concentration is 0.5% for multiple immunostaining with peroxidase labeling for TRPV1 and immunogold labeling for GABA.

• Nutrition Research and Practice
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• 제14권3호
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• pp.286-297
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• 2020

BACKGROUND/OBJECTIVES: This study compared the perception of customers from Korea and the U.S. on the attributes of different formats of menu labeling The specific objectives were 1) to compare the customers' perceived usefulness, ease-of-understanding, clarity, and attractiveness of different formats of menu labeling between Korea and the U.S.; and 2) to compare the customers' use intention to different formats of menu labeling between Korea and the U.S. SUBJECTS/METHODS: A survey was conducted in Korea and the U.S. The participants were allocated randomly to view 1 of the 7 restaurant menus that varied according to the following types of menu labeling formats: (type 1) kcal format, (type 2) traffic-light format, (type 3) percent daily intake (%DI) format, (type 4) kcal + traffic-light format, (type 5) kcal + %DI format, (type 6) traffic-light + %DI format, and (type 7) kcal + traffic-light + %DI format. A total of 279 Koreans and 347 Americans were entered in the analysis. An independent t-test and 1-way analysis of variance were performed. RESULTS: Koreans rated type 4 format (kcal + traffic light) the highest for usefulness and attractiveness. In contrast, Americans rated type 7 (kcal + traffic light + %DI) the highest for usefulness, ease-of-understanding, attractiveness, and clarity. Significant differences were found in the customers' perceived attributes to menu labeling between Korea and the U.S. Americans perceived higher for all the 4 attributes of menu labeling than Koreans. CONCLUSIONS: The study is unique in identifying the differences in the attributes of different formats of menu labeling between Korea and the U.S. Americans rated the most complicated type of menu labeling as the highest perception for the attributes, and showed a higher use intention of menu labeling than Koreans. This study contributes to academia and industry for practicing menu labeling in different countries using different formats.

• Journal of information and communication convergence engineering
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• 제6권2호
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• pp.204-206
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• 2008

In this paper, we proposed the recognition method that automatically extracts cracks from a surface image acquired by a digital camera and recognizes the directions (horizontal, vertical, -45 degree, and 45 degree) of cracks using the fuzzy single layer perceptron. We compensate an effect of light on a concrete surface image by applying the closing operation, which is one of the morphological techniques, extract the edges of cracks by Sobel masking, and binarize the image by applying the iterated binarization technique. Two times of noise reduction are applied to the binary image for effective noise elimination. After the specific regions of cracks are automatically extracted from the preprocessed image by applying Glassfire labeling algorithm to the extracted crack image, the cracks of the specific region are enlarged or reduced to $30{\times}30$ pixels and then used as input patterns to the fuzzy single layer perceptron. The experiments using concrete crack images showed that the cracks in the concrete crack images were effectively extracted and the fuzzy single layer perceptron was effective in the recognition of the extracted cracks directions.

• 한국전기전자재료학회논문지
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• 제19권10호
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• pp.960-965
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• 2006

This research aims to develop the multiple channel electrochemical DNA chip using microfabrication technology. At first, we fabricated a high integration type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the gold electrodes. Then target DNAs were hybridized and reacted. Cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. Therefore, it is able to detect a plural genes electrochemically after immobilization of a plural probe DNA and hybridization of non-labeling target DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

• 한국조리학회지
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• 제24권2호
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• pp.16-22
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• 2018

This study was conducted to investigate that the nutrition labeling use is associated with demographic and psychosocial factors according to each nutrition information on the nutrition labeling in Korean adults. The study subjects (N=1,140) were individuals who were aged 20 years and more and answered on the question of nutrition label use and who participated in the Korean National Health Examination and Nutrition Survey (KNHANES) in 2010.As age older, there was more interest in information such as sugar, protein, fat, cholesterol than calories. In contrast, as age younger, there was more interest intrans-fat, sodium as well as calories. As higher education level, there were more aware of trans-fat, sodium and calories. From the result that the most interested nutrition items were significantly different by democratic factors, we could understand interested nutrient information on the nutrition labels could change according to individual specific education. Therefore, this can also provide basic data for systematic education program by nutrition label use.

• 한국자기공명학회논문지
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• 제4권2호
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• pp.133-139
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• 2000

The strategy to incorporate [$^{15}$ N]-labeled amino acids were discussed. Instead of using specific auxotrophic strains for selective labeling, the prototrophic strain, BL2l(DE3), was used with a plasmid, pLysS, and found to be very effective for several amino acids including alanine, lysine, leucine, and threonine. Isoleucine, valine, glutamine, and tyrosine were also found to be effective despite some diffusion into other amino acids. Interesting result was obtained when [$^{15}$ N]-labeled glycine was tried: only glycines were labeled when amino acid mixture was added in the growth medium, and serines were co-labeled when amino acids were omitted. These results can be used as a guideline when selective labeling strategy is considered, and when the resulting data are interpreted.

• Bulletin of the Korean Chemical Society
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• 제31권6호
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• pp.1474-1478
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• 2010

An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag ($His_6$-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) ($NTA{\cdot}Ni^{2+}$) on the QD surfaces. We showed that the $NTA{\cdot}Ni^{2+}$-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted $His_6$-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.

• 정보처리학회논문지B
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• 제19B권1호
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• pp.43-52
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• 2012

의미 역 결정 (Semantic Role Labeling)은 문장의 각 요소들의 의미 관계를 파악하는 연구 분야로써 어휘 중의성 해소와 더불어 자연언어처리에서의 의미 분석에서 매우 중요한 위치를 차지하고 있다. 그러나 한국어의 경우에는 의미 역 결정에 필요한 언어 자원이 구축되지 못하여 연구의 진행이 매우 미진한 상황이다. 본 논문에서는 의미 역 결정에 필요한 언어 자원 중에서 가장 널리 사용되고 있는 PropBank의 한국어 버전의 구축을 위한 시작 단계로써 자동 술어-논항 분석기를 개발하였다. 자동 술어-논항 분석기는 크게 의미 어휘 사전과 자동 술어-논항 추출기로 구성된다. 의미 어휘 사전은 한국어 동사의 격틀 정보를 구축한 사전이며 자동 술어-논항 추출기는 구문 표지 부착된 말뭉치로부터 특정 술어와 관련있는 논항의 의미 부류를 결정하는 모듈이다. 본 논문에서 개발된 자동 술어-논항 분석기는 향후 한국어 PropBank의 구축을 용이하게 할 것이며, 궁극적으로는 한국어 의미 역 결정에 큰 역할을 할 것이다.

• 한국식품위생안전성학회지
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• 제27권4호
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• pp.325-331
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• 2012

Food allergy refers to an immunologically mediated adverse reaction to food, mainly to proteinaceous constituents. Health implications vary between those individuals who experience mild physical discomforts to those with fast-acting, life-threatening anaphylactic reactions. The prevalence of food allergy is higher in children than in adults, estimated around 4-8% and 1-2% respectively in developed countries. Food allergy has no effective cure at the present time and total avoidance of causative foods is the most reliable prophylactic method currently recommended by the medical community. To help food allergic patients to make informed choices of their foods, mandatory labeling of selected food allergens has been introduced in several countries. All food allergen labelling provisions specify a set of allergens common to the regulated countries. Policy divergence, however, exists between countries by inclusion of additional allergens unique to specific countries and enforcement of specific labelling requirements. Such variations in food allergen labelling regulations make it difficult to manage allergen labeling in imported pre-packaged food products. This paper addresses two current issues in food allergen regulation: 1) an urgent need to determine true prevalence of food allergy in the Asia-Pacific region. This will enable refinement to the food allergen regulation to be more country-specific rather than simply adopting CODEX recommendations. 2) There is an urgent need for harmonization of food allergen regulation in order to prevent food allergen regulation becoming a trade barrier.

• 한국정보과학회논문지:데이타베이스
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• 제32권5호
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• pp.526-535
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• 2005

온톨로지는 특정 개념에 대한 부가정보 및 개념간의 관계를 기술하는 방법으로서 고차원의 웹과 서비스를 실현하기 위한 시멘틱 웹, 그리고 지식관리 시스템을 비롯한 다양한 응용분야의 요구와 관심이 증가하면서 그 중요성이 대두되고 있다. 온톨로지에서 정보에 대한 접근은 특정 개념과 특정 관계를 가지는 데이타를 찾는 것이 주를 이루는데, 이러한 관계가 주로 트랜지티브 관계이기 때문에 트랜지티브 질의를 처리하는 것이 많은 비중을 차지한다. 또한 이와 같은 트랜지티브 클로저 질의 처리는 재귀호출의 형태로서 그 처리 비용 또한 매우 크다. 본 논문에서는 이와 같은 트랜지티브 클로저 질의의 효율적 처리를 지원하기 위한 방법으로써 그래프 레이블링을 이용한 전처리 기법을 제안한다 이 기법은 저장 공간을 효율적으로 사용하고 알고리즘도 단순한 특징을 가지기 때문에 트랜지티브 클로저 질의에 대한 응답 시간을 줄이는 장점을 가지게 된다. 그리고 이와 같이 제안한 기법에 대해 기존 시스템들과 비교해 봄으로써 그래프 레이블링을 이용한 기법이 대용량 온톨로지에서의 트랜지티브 클로저 질의 처리에 효율적임을 보이고자 한다.