• 제목/요약/키워드: specific field

검색결과 3,188건 처리시간 0.023초

SPF 닭에서 재조합 H9N3 조류 인플루엔자 백신의 효능과 안전성 평가

  • 신정화;모인필
    • 한국가금학회:학술대회논문집
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    • 한국가금학회 2006년도 제23차 정기총회 및 학술발표회
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    • pp.90-91
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    • 2006
  • To reduce the economic impact and control Low pathogenic avian influenza (LPAI), vaccination with inactivated vaccine has been considered in this country. We tried to develop inactivated vaccine with reassorted H9N3 AI virus which has different type of neuraminidase compare to those of field AI virus. Before reassorted vaccine was produced, we confirm the virus as master seed by limiting dilution, RT-PCR and sequencing method. Also, we evaluate the biological characteristics of the virus to find out the possibility of prevention against field infection of AI virus. Finally, we evaluate the safety and efficacy of the vaccine made of reassorted AI virus in the specific pathogen free (SPF) chickens. After limiting dilution, we choose RV7CE4 as a vaccine candidate and compare the gene sequence of this vaccine strain to those of AI05GA which is parents strain. Compared to amino acid sequences of specific gene of AI05GA and RV7CE4, exhibited a high degree of amino acid sequence homology. In the safety and efficacy test, there were no specific clinical signs or mortality. Reassorted H9N3 viruses were reisolated in cloaca swab on 5 days post inoculation. In the vaccine study, once or twice vaccination was performed and challenged with H9N2 field virus (01310). Vaccine has no adverse effect on birds and formed good immune capability which reduce viral shedding in the birds infected with 01310. Based on the above result, we developed reassorted H9N3 vaccine which will efficiently prevent the low pathogenic AIV (H9N2) infection in the poultry farms.

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A Reliable "Direct from Field" PCR Method for Identification of Mycorrhizal Fungi from Associated Roots

  • Kuhnann, Christoph;Kim, Seak-Jin;Lee, Sang-Sun;Harms, Carsten
    • Mycobiology
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    • 제31권4호
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    • pp.196-199
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    • 2003
  • A very reliable and specific method for the identification of fungi in ectotrophic mycorrhizal symbiosis was developed using a specific PCR assay based on the amplification of the ITS1 region. To obtain specific data, an ITS-diagnostic assay was carried out that reveals genera and species specific sequences. Here, an application of one method is presented, which covers the identification of pure mycelia, basidiocarps as well as mixed samples such as ectomycorrhizal roots that were mingled with remains of the host plant. For this purpose a protocol was established that allowed the extraction of DNA from single mycorrhizal roots. In order to perform a specific ITS analysis we generated a new ITS-primer(ITS8) by a multiple alignment of five different genera and species of mycorrhizal fungi. The utilization of ITS1 and ITS8 resulted in specific PCR amplicons, which were characterized by sequencing without purification steps, even when the template DNA was associated with roots.

실제조건에서 기준 밀도계와 가스 분석기에 의한 밀도 측정 결과 비교 (Density Measurement Comparisons of Specific Gravity Meter and Gas Chromatography in the Field)

  • 이강진;허재영;하영철
    • 유체기계공업학회:학술대회논문집
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    • 유체기계공업학회 1999년도 유체기계 연구개발 발표회 논문집
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    • pp.90-96
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    • 1999
  • In contracts for sales of natural gas between sellers and buyers, it is not suncient to only measure a volumetric quantity of gas in flowing conditions in metering station. Therefore the measured volumetric quantity must be converted to that of reference conditions. The density of the natural gas required in such a calculation can be measured directly or estimated from the equation of sate or any other experimental methods. The specific gravity meter is the apparatus used to measure the density of fluids under the reference conditions and it can be widely used in industrial areas, especially in massive flow rate natural gas industry. This study has been carried out in an attempt to improve measurement accuracy of natural gas flow rate calculation, providing the adequate installation and proper operation conditions of specific gravity meter. The test results are 1) the density measurement errors in case of using methane and standard gas as calibration gases are smaller than using methane and nitrogen gas, 2) the periodical calibration to maintain accurate density measurements is essential, and 3) the specific gravity meter is sensitive to changes of environmental conditions, especially environmental temperature surrounding the specific gravity meter.

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재한 중국인 유학생의 패션 분야 연구 동향 - 대학원 학위논문을 중심으로 - (Research Trends of Fashion Field among Chinese Students in Korea - Focused on Graduate Degree Thesis -)

  • 워이페이;박은경
    • 복식
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    • 제66권1호
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    • pp.58-72
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    • 2016
  • Since the mid 2000s, a large number of Chinese students have come to Korea to study. This study investigates the research trends of Chinese students studying in the fashion field in Korea. For this study, a total of 235 graduate degree theses on fashion written by Chinese students in Korea (from 1992 to 2014) were collected through the RISS website. Various keywords were used to find the theses, including fashion, clothing and costume. Factors used in the analysis of these theses were the number of theses per year, major of the student, research trend of specific areas and research target area. The results are as follows: Most of the theses were written by Fashion/Clothing majors (141 theses/60% of the total), while other majors - such as Business Administration, International Trade, Economics, Journalism/Broadcasting, and Movie Entertainment - made up the rest (94/40%). The theses researched in the study were focused on a specific field in fashion. Fashion Marketing/Socio-Psychology of Clothing was the most popular field (113/48.1%), and Fashion Design/Aesthetics came in second (87/37.0%). Other topics, such as Costume History, Clothing Construction/Textile Science, Costume Culture, followed. Chinese student's research target area was very limited, with Chinese Study being the most popular area, and Korean and Chinese Comparative Study coming in second.

A study on the effect of the condition number in the magnetic field mapping of the Air-Core solenoid

  • Huang, Li;Lee, Sangjin
    • 한국초전도ㆍ저온공학회논문지
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    • 제17권2호
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    • pp.31-35
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    • 2015
  • Mapping is a useful tool in the magnetic field analysis and design. In some specific research area, such as the nuclear magnetic resonance (NMR) or the magnetic resonance imaging (MRI), it is important to map the magnetic field in the interesting space with high accuracy. In this paper, an indirect mapping method in the center volume of an air-core solenoid is presented, based on the solution of the Laplace's equation for the field. Through the mathematical analysis on the mapping calculation, we know that the condition number of the matrix, generated by the measurement points, can greatly affect the error of mapping result. Two different arrangement methods of the measurement points in field mapping are described in this paper: helical cylindrical line (HCL) method and parallel cylindrical line (PCL) method. According to the condition number, the HCL method is recommended to measure the field components using one probe. As a simple example, we mapped the magnetic fields in a MRI main magnet system. Comparing the results in the different methods, it is feasible and convenient to apply the condition number to reduce the error in the field mapping calculation. Finally, some guidelines were presented for the magnetic field mapping in the center volume of the air-core solenoid.

Effects of curvature radius on vulnerability of curved bridges subjected to near and far-field strong ground motions

  • Naseri, Ali;Roshan, Alireza MirzaGoltabar;Pahlavan, Hossein;Amiri, Gholamreza Ghodrati
    • Structural Monitoring and Maintenance
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    • 제7권4호
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    • pp.367-392
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    • 2020
  • The specific characteristics of near-field earthquake records can lead to different dynamic responses of bridges compared to far-field records. However, the effect of near-field strong ground motion has often been neglected in the seismic performance assessment of the bridges. Furthermore, damage to horizontally curved multi-frame RC box-girder bridges in the past earthquakes has intensified the potential of seismic vulnerability of these structures due to their distinctive dynamic behavior. Based on the nonlinear time history analyses in OpenSEES, this article, assesses the effects of near-field versus far-field earthquakes on the seismic performance of horizontally curved multi-frame RC box-girder bridges by accounting the vertical component of the earthquake records. Analytical seismic fragility curves have been derived thru considering uncertainties in the earthquake records, material and geometric properties of bridges. The findings indicate that near-field effects reasonably increase the seismic vulnerability in this bridge sub-class. The results pave the way for future regional risk assessments regarding the importance of either including or excluding near-field effects on the seismic performance of horizontally curved bridges.

농장별 기상위험 조기경보를 위한 모바일 어플리케이션 설계 및 구현 (Design and Implementation of Mobile Application for Field-specific Early Warning of Agrometeorological Hazards)

  • 신용순;박주현;김성기;강위수;한용규;김대준;김수옥;김진희;심교문
    • 한국농림기상학회지
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    • 제19권3호
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    • pp.180-194
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    • 2017
  • 기상위험에 선제적으로 대응할 수 있도록 하기 위하여 농장별 정밀 날씨추정, 작목별 기상위험의 알림과 대응지침의 전달 등 개별 농가 단위의 농업기상위험 관리 기술이 본 연구의 선행 성과물로 개발된 바 있다. 현재 470 자원 농가와 950농장을 대상으로 시군 단위로 관내 농장의 날씨와 기상위험 현황을 시각적으로 파악할 수 있는 GIS 기반 분포형 경보 시스템이 구축되어 현업 서비스가 운영 중이다(http://www.agmet.kr). 그러나 모든 농가를 서비스 대상으로 하다 보니 많은 작목과 기상위험요소가 나열되어 개별 농가가 시스템에 접속하여 자기 농장만의 농업기상위험 정보를 확인하기에는 불편한 사항이 많다. 따라서 개별 농장별로 정보 확인 편의성을 위하여 휴대전화 번호만을 이용하여 자기 농장만의 날씨, 작목의 생육단계, 작목 기상위험을 최대 미래 9일까지 조회하고, 기상특보 및 기상위험 주의, 경보 수준을 알림 정보로 제공받을 수 있는 농업기상재해 조기경보 모바일용 어플리케이션을 개발하였다. 스마트폰으로 본 시스템에 접속하여 농장별 날씨와 재배작목의 생육단계, 생육단계별 기상위험과 대응지침을 제공 받는 등 농장별로 특화된 기상위험을 사전 경고 정보로 신속하게 확인할 수 있다. 자기 농장만의 과거부터 미래까지 날씨와 기상위험을 단순하고 보기 쉽게 정리하여 농가에 제공함으로써 기존의 GIS 기반 기상위험 분포형 경보 시스템이 가진 정보 접근 불편함을 해소하였다.

벼 재배 포장 생육변이의 공간통계학적 해석 (Geo-statistical Analysis of Growth Variability in Rice Paddy Field)

  • 이충근;성제훈;정인규;김상철;박우풍;이용범;박원규
    • Journal of Biosystems Engineering
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    • 제29권2호
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    • pp.109-120
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    • 2004
  • To obtain basic information for precision agriculture, spatial variability of rice growth condition was evaluated in 100m ${\times}$100m paddy field. The rice growth condition of four hundred locations in the field were investigated to analyze the spatial variability of their properties ; SPAD, plant length and tiller number. Geostatistical analysis was carried out to examine within-field spatial variability using semivariograms and kriged maps as well as descriptive statistics. Descriptive statistics showed that the coefficient of variation for SPAD, plant length, and tiller number exceeded 5.70 %, suggesting a relatively high variability. Geostatistical analysis indicated a high spatial dependence for all the properties except for the second tiller number. The range of spatial dependence was about 20 m for SPAD, plant length, and tiller number. Based on the results of spatial dependence, kriged maps were prepared for the properties to analyse their spatial distribution in the field. The results reflected the history of field management. In conclusion, the need for site-specific field management and possibility of precision agriculture were demonstrated even in an almost flat paddy field.

박막 트랜지스터 기판 검사를 위한 PDLC 응용 전기-광학 변환기의 동특성 분석 (Dynamic Analysis of the PDLC-based Electro-Optic Modulator for Fault Identification of TFT-LCD)

  • 정광석;정대화;방규용
    • 한국정밀공학회지
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    • 제20권4호
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    • pp.92-102
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    • 2003
  • To detect electrical faults of a TFT (Thin Film Transistor) panel for the LCD (Liquid Crystal Display), techniques of converting electric field to an image are used One of them is the PDLC (polymer-dispersed liquid crystal) modulator which changes light transmittance under electric field. The advantage of PDLC modulator in the electric field detection is that it can be used without physically contacting the TFT panel surface. Specific pattern signals are applied to the data and gate electrodes of the panel to charge the pixel electrodes and the image sensor detects the change of transmittance of PDLC positioned in proximity distance above the pixel electrodes. The image represents the status of electric field reflected on the PDLC so that the characteristic of the PDLC itself plays an important role to accurately quantify the defects of TFT panel. In this paper, the image of the PDLC modulator caused by the change of electric field of the pixel electrodes on the TFT panel is acquired and how the characteristics of PDLC reflect the change of electric field to the image is analyzed. When the holding time of PDLC is short, better contrast of electric field image can be obtained by changing the instance of applying the driving voltage to the PDLC.

Critical current characteristic of various 2G HTS multi-stacked tapes depending on the low external magnetic field

  • Kim, J.;Lee, W.S.;Jin, H.;Ko, T.K.
    • 한국초전도ㆍ저온공학회논문지
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    • 제16권1호
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    • pp.27-31
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    • 2014
  • 2G HTS tapes are widely used for various electric machines. In addition, stacked or parallel connected HTS tapes are essentially used to raise transport current level for large capacity electric machines. Therefore, critical current characteristic of stacked tapes need to be studied. Recently developed 2G HTS tapes are fabricated with various defects doping so that tapes possess pinning center to improve the critical current characteristic. During this process, the critical current is determined minimum value in not perpendicular magnetic field but a specific magnetic field angle according to the reported research. However, the effects of magnetic field angle to critical current of multi-stacked 2G HTS tapes have not been examined. In this paper, field coil which is a race-track coil wound by using an HTS tape with iron-core was fabricated to apply angle adjustable magnetic field to the 2G HTS tape samples. We measured critical current of single and multi-stacked two tapes that have different characteristic depending on various magnetic field angle and magnitude in liquid nitrogen environment. Furthermore, results of single and multi-stacked tapes were compared and analyzed.