• Korean Journal of Veterinary Research
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• v.38 no.2
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• pp.304-313
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• 1998

Sixteen Korean field transmissible gastroenteritis viruses (TGEVs) were isolated using swine testicular cell (STC) and the genomic diversity of them was analyzed. All TGEV isolates produced a typical cytopathic effect in STC and were confirmed as TGEV by immunofluorescence assay using monoclonal antibody against TGEV and PCR using TGEV specific primers. RNAs from TGEV field isolates and vaccine TGEV were extracted and amplified by RT and PCR. The RT-PCR products were digested with selected restriction enzymes and analyzed RFLP patterns. The N-terminal end region of S gene and ORF 3 and 3-1 genes of TGEV amplified by TGEV specific primer pairs seemed to be conserved. Most specific variations were detected in S gene amplified by TGEV 4/6 primer pairs which includes antigenic sites A and D. When the PCR products were treated with Sau3AI and Ssp I, Bvac(vaccine strain), field isolates 133 and 347 were differentiated from Miller and Purdue types. In the case of D5 field isolates, it was classified into Purdue type by Sau 3AI but classified into independent TGEV by Ssp I. Two different TGEV strains from D2 sample were confirmed by plaque purification and RT-PCR-RFLP analysis. To investigate the change occurring in TGEV genome after serial passage, the TGEV P44 strain was passaged through STC. There were specific changes in S gene and a large deletion was observed in ORF 3 and 3-1 genes. These studies showed that a distinct difference in genome exists among TGEV field isolates.

• Journal of Biosystems Engineering
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• v.31 no.1 s.114
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• pp.24-32
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• 2006

Compaction is becoming a greater concern in crop production and the environment because it can have deleterious effects on growing conditions that are difficult to remediate. Because compaction can vary considerably from point to point within a field, and also from depth to depth within the soil profile, it is important to consider quantification and management of the spatial and vertical variability in soil compaction when developing an overall site-specific crop management plan. In this paper, the importance of soil compaction, techniques for quantification of its variability, and the concept of site-specific tillage are examined. Methods and systems to detect within-field variation in soil strength as a surrogate measure of soil compaction and related soil properties are also compared and discussed. Quantification of variability in soil compaction and site-specific compaction management was motivated recently, and sensors and control systems are still under development. Future study will need to address a number of issues related to understanding and applying the sensor measurements.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 1999.10c
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• pp.753-758
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• 1999

Concentration and discharge have been intensively monitored at the drainage canal in a paddy field area during storm-periods. Among 4 storm runoffs, the No. 2 and No. 3 runoff was in the fertilizer application period. The specific load-specific discharge equation L=aQ\ulcorner have different characteristics for the pollutants. The coefficient of b generally shows values of more than 1 for T-N, about 1 for COD\ulcorner, and less than 1 for T-P. For same specific discharge, No. 2 runoff shows higher specific load than other runoffs. For the coefficient of determination of the L-Q equation, COD\ulcorner is higher than T-N and T-P. The mean concentration of direct runoff, significantly depending on the storm events, is 0.6 to 8.3mg/ιfor T-N, 0.05 to 0.51 mg/ι for T-P, and 10.0 to 18.3 mg/ι for COD\ulcorner.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2010.05a
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• pp.219-220
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• 2010

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1998.10a
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• pp.345-352
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• 1998

Free-field ground motion during earthquake is significantly affected by the local site conditions and the behavior of structure is influenced by ground motion, it is essential to perform the reliable site characterization and to determine the site specific earthquake response. In this study, case study of site specific ground response evaluation was performed at Inchon area. Step by step procedures for site characterization and one-dimensional site response analysis were introduced and the importance of site specific analysis was verified.

• BMB Reports
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• v.40 no.2
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• pp.142-150
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• 2007

Epigenetic alterations, represented by aberrant DNA methylation, are deeply involved in human cancers. In gastric cancers, tumor-suppressor genes are inactivated more frequently by promoter methylation than by mutations. We recently showed that H. pylori infection, a potent gastric carcinogenic factor, induces methylation of specific genes in the gastric mucosae. When the methylation levels were analyzed in the gastric mucosae of healthy volunteers, cases with a single gastric cancer, and cases with multiple gastric cancers, who have increasing levels of risks for gastric cancers, there was a significant increasing trend in the methylation levels among the individuals without current H. pylori infection. This finding unequivocally showed the presence of an epigenetic field for cancerization. The degree of the field defect was measured more conveniently using methylation levels of marker genes than using those of tumor-suppressor genes. The presence of an epigenetic field for cancerization has been indicated for liver, colon, Barrett's esophageal, lung, breast, and renal cancers. Since decreased transcription is involved in the specificity of methylated genes, it is likely that specific genes are methylated according to carcinogenic factors. These findings emphasize the usefulness of DNA methylation as a marker for past exposure to carcinogens and future risk of cancer development.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.8 s.227
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• pp.1135-1139
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• 2004

This paper presents a microextractor for the separation of DNA molecules by their sizes. The DNA microextractor immobilizes the DNA molecules of specific size in the micropillar array by adjusting the period of the crossed electric field, thus providing a starting-point independent target DNA extraction method without separation process monitoring. The DNA microextractor has been fabricated by a three-mask micromachining process. The velocity of three different DNA molecules has been measured at the electric field of E=5V/0.8cm in the fabricated DNA microextractor, resulting in the reorientation times of $4.80{\pm}0.44sec,\;7.12{\pm}0.75sec$, and $9.88{\pm}0.30sec$ for ${\lambda}$ DNA, micrococcus DNA, and T4 DNA, respectively. T4 DNA is trapped in the micropillar array when the crossed electric field of 5V/0.8cm is applied alternately at a 10 second time interval. The present DNA microextractor filters the DNA in a specific size range by adjusting the magnitude and/or the period of the crossed electric field applied in the micropillar array.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.5
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• pp.2680-2700
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• 2017

Finite field arithmetic over GF($2^m$) is used in a variety of applications such as cryptography, coding theory, computer algebra. It is mainly used in various cryptographic algorithms such as the Elliptic Curve Cryptography (ECC), Advanced Encryption Standard (AES), Twofish etc. The multiplication in a finite field is considered as highly complex and resource consuming operation in such applications. Many algorithms and architectures are proposed in the literature to obtain efficient multiplication operation in both hardware and software. In this paper, a modified serial multiplication algorithm with interleaved modular reduction is proposed, which allows for an efficient realization of a sequential polynomial basis multiplier. The proposed sequential multiplier supports multiplication of any two arbitrary finite field elements over GF($2^m$) for generic irreducible polynomials, therefore made versatile. Estimation of area and time complexities of the proposed sequential multiplier is performed and comparison with existing sequential multipliers is presented. The proposed sequential multiplier achieves 50% reduction in area-delay product over the best of existing sequential multipliers for m = 163, indicating an efficient design in terms of both area and delay. The Application Specific Integrated Circuit (ASIC) and the Field Programmable Gate Array (FPGA) implementation results indicate a significantly less power-delay and area-delay products of the proposed sequential multiplier over existing multipliers.

• The Plant Pathology Journal
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• v.24 no.1
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• pp.58-62
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• 2008

Utilization of carbon sources by Bacillus cereus D324, a biological control agent, and Pythium species, which causes rice seedling disease, was studied with the objective of increasing the efficacy of biological control by providing the biological control agent with specific beneficial carbon sources. D-galactose, D-sorbitol, and D-mannitol were poor carbon sources for Pythium spp. growth but were good for B. cereus D324 growth. Growth in a growth chamber of rice seeds coated with B. cereus D324 amended with specific carbon sources, such as D-galactose and D-sorbitol, showed significantly enhanced seedling emergence compared to seeds coated only with B. cereus D324. Field trials showed that both seedling emergence and yield increased, when the above specific carbon sources were added to B. cereus D324 in seed coating formulations. This result indicated that amending seed coating formulations with specific carbon sources could significantly increase seedling emergence and yield in the field.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.29 no.7 s.238
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• pp.814-819
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• 2005

The phase change heat transfer has been applied to the processes of machines as well as of manufacturing. The cycle in a heat exchanger includes the phase change phenomena of coolant for air conditioning, the solidification in casting process makes use of the characteristics of phase change of metal, and the welding also proceeds with melting and solidification. To predict the phase change processes, the experimental and numerical approaches are available. In the case of numerical analysis, the Enthalpy method is most widely applied to the phase change problem, comparing to the other numerical methods, i.e. the Equivalent Specific Heat method and the Temperature Recovery method. It's because that the Enthalpy method is accurate and straightforward. The Enthalpy method does not include any correction step while the correction of final temperature field is inevitable in the Equivalent Specific Heat method and the Temperature Recovery method. When the temperature field is to be used in the calculation, however, there must be converting process from enthalpy to temperature in the calculation scheme of Enthalpy method. In this study, an improved method for the Equivalent Specific Heat method is introduced whose method dose not include the correction steps and takes temperature as an independent variable so that the converting between enthalpy and temperature does not need any more. The improved method is applied to the solidification process of pure metal to see the differences of conventional and improved methods.