• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1994년도 제2회 추계심포지움
• /
• pp.69-80
• /
• 1994

Although ureases play important roles in microbial nitrogen metabolism and in the pathogenesis of several human diseases, little is known of the mechanism of metallocenter biosynthesis in this Ni-Containing enzyme. Klebsiella aerogenes urease apo-protein was purified from cells grown in the absence of Ni. The purified apo-enzyme showed the same native molecular weight, charge, and subunit stoichiometry as the holo-enzyme. Chemical modification studies were consistent with histidinyl ligation of Ni. Apo-enzyme could not be activated by simple addition of Ni ions suggesting a requirement for a cellular factor. Deletion analysis showed that four accessory genes (ureD, ureE, ureF, and ureG) are necessary for the functional incorporation of the urease metallocenter. Whereas the $\Delta$ureD, $\Delta$ureF, and $\Delta$ureG mutants are inactive and their ureases lack Ni, the $\Delta$ureE mutants retain partial activity and their ureases possess corresponding lower levels of Ni. UreE and UreG peptides were identified by SDS-polyacrylamide gel comparisons of mutant and wild type cells and by N-terminal sequencing. UreD and UreF peptides, which are synthesized at ve교 low levels, were identified by using in vitro transcription/translation methods. Cotransformation of E. coli cells with the complementing plasmids confirmed that ureD and ureF gene products act in trans. UreE was purified and characterized. immunogold electron microscopic studies were used to localize UreE to the cytoplasm. Equilibrium dialysis studies of purified UreE with $^{63}$ NiC1$_2$ showed that it binds ～6 Ni in a specific manner with a $K_{d}$ of 9.6 $\pm$1.3 $\mu$M. Results from spectroscopic studies demonstrated that Ni ions are ligated by 5 histidinyl residues and a sixth N or O atom, consistent with participation of the polyhistidine tail at the carboxyl termini of the dimeric UreE in Ni binding. With these results and other known features of the urease-related gene products, a model for urease metallocenter biosynthesis is proposed in which UreE binds Ni and acts as a Ni donor to the urease apo-protein while UreG binds ATP and couples its Hydrolysis to the Ni incorporation process.ouples its Hydrolysis to the Ni incorporation process.s.

• 한국의류산업학회지
• /
• 제18권5호
• /
• pp.658-667
• /
• 2016

The purpose of this study is 1) to research how practitioners in fashion industry in South Korea perceive concepts of Technical Design/Designer, 2) to compare and analyze issues at work by occupation, 3) to research specific works in garment development process, and 4) to compare and analyze work involvement by occupation, type of a company and etc, and 5) to propose the role of Technical Designers in apparel companies in South Korea. There were two methods to conduct this study, which were in-depth interview and survey. Both methods were conducted to designers, merchandisers, pattern makers, technical designers, and production coordinators. Frequency analysis, ANOVA, Duncan test, and Factor analysis were performed to get results by using SPSS 18.0 program. The results are following. There were 50 works during garment development process from the result of in-depth interview, and 6 factors were obtained from the result of Factor analysis, which were 'Works about Sample in Sample Development Process', 'Works about Product's Pattern and Size Spec', 'Works about Development of Garment's Design', 'Works about Planning of Product Development and Management of Product in Stock', 'Works about Production Process', and 'Preparation Works for Sample Development'. In conclusion, technical designer in apparel companies in South Korea should be in charge of works about sample in sample development process and decision making of product' size spec, which is included in works about product's pattern and size spec. Also, they should complete technical package after product is developed by designers.

• 정보처리학회논문지C
• /
• 제11C권7호
• /
• pp.937-950
• /
• 2004

본 논문에서는 프로토콜 조합 및 서비스 참조 기법을 지원하는 Customizable Architecture for Flexible Execution Environment(CAFEs) 실행환경을 실제 및 구현한다. CAFEs 실행환경은 프로토콜 및 서비스 소프트웨어의 재사용성을 높이기 위하여 기존 프로토콜의 기능 또는 알고리즘을 구현한 마이크로 프로토콜과 기존 응용과 호환하고 액티브 네트워크 서비스를 배포하기 위한 수단으로 동작하는 액티브 서비스라는 새로운 개념을 제시한다. 제안된 액티브 네트워크 실행환경은 기능에 따라 세 부분으로 설계되어 있다. 첫째, 가상 네트워크 시스템은 기존의 다양한 프로토콜 계층을 기반으로 가상 네트워크 채널을 형성하고 이를 바탕으로 액티브 노드를 연결한다. 둘째, 프로토콜 및 서비스 관리자는 마이크로 프로토콜 및 액티브 서비스를 합성함으로써 새로운 프로토콜과 서비스를 제공하는 역할을 담당한다. 마지막으로 이벤트 엔진은 이벤트 위임 모델을 사용하여 서비스에 존재하는 컴보넌트의 상태전이를 자동으로 감지한다. 그리고 개발된 CAFEs 실행환경을 검증하기 위하여 무선 인터넷 환경에서 사용자 단말에 적합한 웹 컨텐츠를 전달하기 위한 실험을 수행하여 가용성을 확보하였다.

• 디지털융복합연구
• /
• 제18권10호
• /
• pp.479-490
• /
• 2020

본 연구는 정신재활시설에서 진행하고 있는 정신장애인 직업재활서비스에 대해 정신재활시설의 직업재활 실무자들은 어떠한 인식을 갖고 있는지 살펴보고, 이를 토대로 정신장애인 직업재활서비스의 활성화 방안을 제시하고자 하였다. 정신재활시설에서 제공하는 직업재활서비스와 관련된 자료를 수집하였고, 직업재활을 담당하고 있는 실무자들을 대상으로 초점집단인터뷰(FGI)를 실시하고, 이 자료를 분석하여 결과를 도출하였다. 연구결과는 다음과 같다. 정신재활시설에서 직업재활서비스의 정체성을 찾도록 매뉴얼과 지침의 정비가 요구되었으며, 직업재활서비스에 참여하는 근로자에게 맞는 세부적인 기준이 필요한 것으로 나타났다. 또한 타 장애인과 동등하고, 정신장애인의 특성에 맞는 직업재활서비스의 운영을 기대하고 있었다. 이러한 연구결과를 토대로, 직업재활서비스의 구체적인 활성화 방안을 제시하였다.

• 한국미생물생명공학회:학술대회논문집
• /
• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
• /
• pp.35-39
• /
• 2001

The Integration Host factor (IHF) of Escherichia coli is a small, basic protein that is required for a variety of functions including site-specific recombination, transposition, gene regulation, plasmid replication, and DNA packaging. It ,is composed of two subunits that are encoded by the ihfA ($\alpha$-subunit) and ihjB ($\beta$-subunit) genes. IHF binding sites are composed of three elements called the WATCAR, TTG, and poly (dAT) elements. We have characterized IHF binding to the H site of bacteriophage λ. We have isolated suppressors that bind to altered H' sites using a challenge phage selection. Two different suppressors were isolated that changed the adjacent $\alpha$P64 and $\alpha$K65 residues. The suppressors recognized both the wild-type site and a site with a change in the WATCAR element. Three suppressors were isolated at $\beta$-E44. These suppressors bound the wild-type and a mutant site with a T：A to A：T change (H44A) in the middle of the TIR element. Site-directed mutagenesis was used to make several additional changes at $\beta$E44. The wild-type and $\beta$E44D mutant could not bind the wild-type site but were able to bind the H44A mutant site. Other mutants with neutral, polar, or a positive charge at $\beta$E44 were able to repress both the wild-type and H44A sites. Examination of the IHF crystal structure suggests that the ability of the wild-type and $\beta$E44D proteins to discriminate between the T：A and A：T basepairs is due to indirect interactions. The $\beta$-E44 residue does not contact the DNA directly. It imposes binding specificity indirectly by interactions with residues that contact the DNA. Details of the proposed interactions are discussed.

• 한국신재생에너지학회:학술대회논문집
• /
• 한국신재생에너지학회 2009년도 추계학술대회 논문집
• /
• pp.158-158
• /
• 2009

The electrophoretic deposition(EPD) technique with a wide range of novel applications in the processing of advanced ceramic materials and coatings, has recently gained increasing interest both in academic and industrial sector not only because of the high versatility of its use with different materials and their combinations but also because of its cost-effectiveness requiring simple apparatus. Compared to other advanced shaping techniques, the EPD process is very versatile since it can be modified easily for a specific application. For example, deposition can be made on flat, cylinderical or any other shaped substrate with only minor charge in electrode design and positioning[1]. The synthesis of the nano-sized Ce0.2Sm0.8O1.9(SDC)particles prepared by aurea based low temperature hydrothermal process was investigated in this study[2].When we made the SDC nanoparticles, changed the time of synthesis of the SDC. The SDC nanoparticles were characterized with field-emission scanning electron microscope(FESEM), energy dispersive X-ray analysis(EDX), and X-ray diffraction(XRD). And also we researched the results of our investigation on electrophoretic deposition(EPD) of the SDC particles from its suspension in acetone solution onto a non-conducting NiO-SDC substrate. In principle, it is possible to carry out electrophoretic deposition on non-conducting substrates. In this case, the EPD of SDC particles on a NiO-SDC substrate was made possible through the use of a adequately porous substrate. The continuous pores in the substrates, when saturated with the solvent, helped in establishing a "conductive path" between the electrode and the particles in suspension[3-4]. Deposition rate was found to increase its increasing deposition time and voltage. After annealing the samples $1400^{\circ}C$, we observed that deposited substrate.

• 한국의류학회지
• /
• 제40권1호
• /
• pp.26-40
• /
• 2016

The importance of Corporate Social Responsibility (CSR) implementation is increasing and CSR activity has become a core activity for firms. The increase on CSR activity expenditures also increases the need for strategic. When firms implement CSR strategically, firms need to consider consumers' perspectives toward CSR. This research investigated how Korean fashion companies integrate consumers' CSR expectations into CSR implementation. Through a qualitative study, we researched a firm's consideration of consumers which are reflected in detailed procedures of CSR implementation. We conducted in-depth interviews with executives and managers in charge of CSR and found the following results. First, motivations for CSR implementation were both strategic and moral along with consumers who play a crucial role in CSR motivation. Second, firms conducted various strategic CSR activities differentiating themselves using a specific resource. Third, self-CSR evaluation was not active and structured. Few companies were collecting consumer responses to CSR activities. Last, executives and managers have difficulties to perform CSR activities due to gaps between ethical consumption consciousness and the ethical consumption behavior, measurement of CSR effect, and lack of understanding of strategic CSR. The results of our study show that firm's consideration of consumers is insufficient despite executives and managers who strongly agree with the need for strategic CSR.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2011년도 제41회 하계 정기 학술대회 초록집
• /
• pp.76-76
• /
• 2011

Polymer-fullerene based bulk heterojunction (BHJ) solar cells can be fabricated in large area using low-cost roll-to-roll manufacturing methods. However, because of the low mobility of the BHJ materials, there is competition between the sweep-out of the photogenerated carriers by the built-in potential and recombination within the thin BHJ film [12-15]. Useful film thicknesses are limited by recombination. Thus, there is a need to increase the absorption by the BHJ film without increasing film thickness. Metal nanoparticles exhibit localized surface plasmon resonances (LSPR) which couple strongly to the incident light. In addition, relatively large metallic nanoparticles can reflect and scatter the light and thereby increase the optical path length within the BHJ film. Thus, the addition of metal nanoparticles into BHJ films offers the possibility of enhanced absorption and correspondingly enhanced photo-generation of mobile carriers. In this work, we have demonstrated several positive effects of shape controlled Au and Ag nanoparticles in organic P3HT/PC70BM, PCDTBT/PC70BM, Si-PCPDTBT/PC70BM BHJ-based PV devices. The use of an optimized concentration of Au and Ag nanomaterials in the BHJ film increases Jsc, FF, and the IPCE. These improvements result from a combination of enhanced light absorption caused by the light scattering of the nanomaterials in an active layer. Some of the metals induce the plasmon light concentration at specific wavelength. Moreover, improved charge transport results in low series resistance.

• 한국광물학회지
• /
• 제14권1호
• /
• pp.12-20
• /
• 2001

충북 영동군 동창광산에서 산출되는 일라이트(illite)광선의 표면화학특성을 전위차 적정 실험과 FITEQL3.2 프로그램을 이용하여 연구하였다. 정량 Xtjs 회절 분석에 의한 일라이트 광석의 광물조석은 석영 46.6% 일라이트 41.6% 카올리나이트(kaolinite) 11.8%이며 $N_2$BET 방법에 의하여 구한 비표면적은 $6.52 m^2$g이다. 전위차 적정 실험결과를 그란(Gran)법을 적용하여 구한 일라이트광석의 영전하점($pH_{pznpc}$ )은 pH 3.9 총표면 자리 밀도는 21.24 sites/$nm^2$이다. 표면 복합체 모델중 일정 용량 모델을 적용해 일라이트 광석의 표변 특성에 알맞는 모델을 찾아보았다. 일라이트 광석의 표면을 사면체 자리와 팔면체 자리로 나누어 설정한 2sites$-3pK_{ a}$s 모델은 변수값이 수렴되지않았으므로 부적절하다고 판단된다. 일라이트 광석의 표면을 하나의 균질한 흡착표면으로 가정해서 설정한 1 site -1 $K_{a}$ 와 1 site -2 $pK_{a}$ s 모델 사이에는 뚜렷한 차이는 없지만, 1 site -1 $pK_{a}$ 모델의 WSOS/DF 값이 17, 1 site - 2 $pK_a{s}$ 모델은 26으로서 앞 모델이 보다 적절하다. 이 결과는 일라이트 광석 표면에서 수소의 해리와 첨가 반응 중 첨가 반응을 무시하여도 표면반응을 설명하는 데 큰 무리가 없음을 시사한다. 가장 적절하다고 판단되는 1 site -1 $pK_{a}$ 모델의 $pK_{a}$ 값은 4.17, specific capacitance는 $6F/m^2$ 표면 자리 농도는 $1.15\Times10^{-3}$ mol/L 이다.

• 박물관보존과학
• /
• 제23권
• /
• pp.31-48
• /
• 2020

야외 석조문화재는 다른 문화재에 비해 양적인 면에서나 규모 면에서의 한계로 인해 많은 인력을 필요로 하는 작업으로 보존처리 회사가 많은 부분을 담당하고 있다. 이 연구에서는 서울지역 관악구에 위치하는 낙성대 삼층석탑을 대상으로 실시한 보존처리 사례를 소개하고자 한다. 이를 위해 석조문화재의 보존 상태와 훼손 현황을 살펴보고 보존처리 사용 재료 및 방법에 대해서 정리하였다. 낙성대 삼층석탑과 같이 도심에 위치하는 석조문화재의 표면오염은 지역적인 특징이 나타나게 되며 이에 따라 보존처리 기법의 적용이 차별화되어 이루어지게 된다. 향후 박물관 소장 야외 석조문화재 뿐만 아니라 서울지역 석조문화재의 보존처리에 참고자료로써 활용되기를 기대한다.