• Animal cells and systems
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• v.6 no.1
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• pp.45-51
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• 2002

We compared the complete mitochondrial cytochrome b gene sequences of Korean and European cobitids to provide independent evidence for assessment of systematic and biogeographic relationships of species in the genus Cobitis. The data suggested monophyly of the genus Cobitis and the inclusion of Korean Cobitis species within the group having one lamina circularis, a primitive condition. Also, all the phylogenetic analyses using maximum parsimony, maximum likelihood, and neighbor joining methods showed a monophyletic relationship among Cobitis. The basal position of the Caspian C. cf. sibirica reported here reflects the eastern Asiatic origin cf. the European Cobitis and establishes C. cf. sibirica as an independent lineage. The Korean C. pacifica diverged next to C. cf. sibirica in basal group from the genus Cobitis. This result is in agreement with the hypothesized Asiatic origin of some European freshwater fish lineages. The phylogenetic relationships in this study showed a close affinity between C. zanadreai and C. sinensis. Two new species, C. tetralineata and C. pacifica in Korea also are closely related to monophyletic group clustering the type species of the Acanestrinia subgenus (C. elongata) with all the endemic Italian species (C. bilineata and C. zanandreai). This may suggest that the affinity between the Korean and Danubian-Italian imply genetic convergence or genetic plesiomorphic state between allopatric species that are separated for the Miocene. The mtDNA-based phylogeny for the species of the genus Cobitis from Kores and Europe permits phylogenetic assessment of the morphological transitions of Iamina circularis.

• Animal cells and systems
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• v.6 no.1
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• pp.13-18
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• 2002

Phylogenetic relationships of Korean Acidiella species were tested using mitochondrial 16S ribosomal RNA gene sequences. We used 16 published sequences as outgroup, and 10 new sequences for nine Korean Acidiella species as ingroup. The number of aligned sites was 1,281 bp, but 1,135 bp were used for the analysis after excluding sites with missing data or gaps. Among these 1,135 sites, 464 sites were variable and 340 were informative for parsimony analysis. Phylogenetic information was extracted from this data set using neighbor-joining, maximum likelihood and maximum parsimony methods and compared to a morphology-based phylogenetic hypothesis. Our molecular data suggest that: (1) the tribe Trypetini appears to be monophyletic even when the nine additional Acidiella species are added to our previous phylogenetic analysis; (2) all the Korean Acidiella species belong to the Trypeta group, but the genus Acidiella is not supported as monophyletic; (3) the close relationship of A. circumvaga, A. issikii, and A. sapporensis is supported; (4) the close relationship of A. pachypogon and two additional new Acidiella species is strongly supported; and (5) the possible presence of two or more cryptic species among the specimens previously identified as A. obscuripennis is suggested. Sequence data from the mitochondrial 16S rDNA allowed us to better understand the systematic status of Korean Acidiella species. They indicated that the current concept about the genus Acidiella is insufficient and needs to be refined further. This study also showed a few interesting relationships, that had not been recognized by morphological study alone. Based on this study, we were able to plan further experiments to analyze relationships within the Trypeta Group.

• Journal of People, Plants, and Environment
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• v.23 no.3
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• pp.293-304
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• 2020

Background and objective: This study was carried out to assess the relationship between the internal and external environments and the ecological items in five sites with a high level of artificial use intensity prior to the establishment of Geumgang River riparian ecological belts. Methods: The sites were classified into forest type, cultivated land type and urbanized type in accordance with their respective locational types. Ecological items including plant ecology such as proportion, naturalization index and urbanization index of native species were analyzed and animal ecology such as the number of species and population of wild birds, amphibians and reptiles, mammals, and butterflies and dragonflies, which are indicator insects, were investigated. In addition, species diversity indices of wild birds, butterflies and dragonflies found in all the subject lands were computed. Results: Among the plant ecological items, the ratio of native species in the forest type was higher than 90%, which was a satisfactory level, while the naturalization and urbanization indices were less than 10%. The number of species in the animal ecological characteristics was reduced in the order of forest type, cultivated land type and urbanized type. As the results of correlation analysis, the internal area of the planted area showed a negative correlation with the total of individuals. The area of grasslands showed a positive correlation with the number of dragonfly species and the total number of individuals, thereby illustrating that wet grasslands have positive effects. The area of surrounding forests, as an external environment, had a negative correlation with the urbanization index (UI) but a positive correlation with the inhabitation of butterflies and the total number of species. Conclusion: The results confirmed the need for more diversified special compositions including planted land, grassland, wetland, bodies of water and waterways within the subject land in the wetland ecological belt along with the need for surrounding forest location and preservation from the perspective of purchase and restoration of land for enhancement of wider biodiversity in the future.

• The Korea Journal of Herbology
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• v.28 no.3
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• pp.75-84
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• 2013

Objectives : The origin of Breeae Herba (So-gye) and Cirsii Herba (Dae-gye) is differently prescribed in Korean and Chinese modern pharmacopoeia. Since the similar morphological characteristics and chaotic plant names, moreover, the aerial part of Carduus crispus have been used as the Cirsii Herba. To develop a reliable method for correct identification of these herbal medicines and to evaluate the genetic relationship of these closely related plant species, we analyzed sequences of DNA barcode regions. Methods : Thirty-one samples of 6 medicinal plants (B. segeta, B. setosa, C. japonicum var. maackii, C. setidens, C. chanroenicum, and C. crispus) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL) were analyzed after amplification using appropriate primers reported in previous studies. The nucleotides of species-specific authentic marker and phylogenetic relations were estimated based on the entire sequences of DNA barcodes by the analysis of ClastalW and UPGMA, respectively. Results : In comparative analysis of DNA barcode sequences, we obtained specific nucleotides to discriminate the medicinal plant of Breeae/Cirsii Herba in species level and evaluated the phylogenetic relationship of these species. Futhermore, we identified distinct marker nucleotides enough to authenticate respective species. These sequence differences at corresponding positions were avaliable genetic markers to determine the botanical origins of Breeae Herbal as well as Cirsii Herba. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by providing of definitive information that can identify each plant species and distinguish from unauthentic adulterants and substitutes.

• The Korea Journal of Herbology
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• v.28 no.6
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• pp.53-58
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• 2013

Objectives : Pinelliae Tuber has been used as a typical unauthentic herbal medicines. Due to the morphological similarity between Pinelliae Tuber and adulterants, the correct authentication is very difficult. Therefore, we introduced DNA barcode to establish a powerful tool for the authentication of Pinelliae Tuner from adulterants. Methods : To obtain DNA barcode regions, genomic DNA was extracted from nineteen specimens of Pinellia ternata, Pinellia pedatisecta, Pinellia tripartita, and Typhonium flagelliforme, and matK and rbcL genes were amplified. For identification of species specific sequences and analysis phylogenetic relationship, a comparative analysis were performed by the ClastalW and UPGMA based on entire sequences of matK and rbcL genes, respectively. Results : In comparison of two DNA barcode sequences, we elucidated the phylogenetic relationship showing distinct four groups depending on species and identified 40 and 20 species specific nucleotides enough to distinguish each species from matK and rbcL gene, respectively. The sequence differences at the corresponding positions were avaliable genetic marker nulceotides to discriminate the correct species among analyzed four species. These results indicated that phylogentic and comparative analysis of matK and rbcL genes are useful genetic markers to authenticate Pinelliae Tubers. Conclusions : The marker nucleotides enough to distinguish P. ternata, P. tripatrita, P. peditisecta, and T. flagelliform, were observed at 40 positions in matK gene and 20 positions in rbcL gene sequence, respectively. These differences can be used to authenticate Pinelliae Tuber from adulterants as well as discriminate each four species.

• Journal of Aquaculture
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• v.21 no.4
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• pp.346-350
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• 2008

RAPD analysis was examined to estimate the availability as a genetic marker. The availability was evaluated in terms of genetic divergence and relationships among Haliotis discus hannai, H. rufescens, H. rubra and H. midae in both hemispheres of the world. In results, RAPD analysis showed a clear genetic divergence between every pair of species. However, genetic relationships among the four species estimated by RAPD analysis unreflected to geographical distribution and morphological characteristics. In conclusion, RAPD is suitable genetic markers for estimates of genetic divergence and differences among abalone species.

• Journal of Korean Society on Water Environment
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• v.29 no.1
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• pp.7-18
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• 2013

We compared the structure of fish community and condition of major fish species in Lake Chungpyung and Lake Paldang, which are relatively shallow, meso-eutrophic, cascading dam reservoirs on the North Han River. Two lakes have wide littoral zone in the lakeside providing similarly good habitat for fishes, whereas fishery and water recreational activities such as motorboating, water skiing are allowed in Lake Chungpyung but are prohibited in Lake Paldang. The average lengths of large size fishes in Lake Chungpyung are shorter than those of same species in Lake Paldang, resulting in the slight distortion of generation distribution of those species in Lake Chungpyung, possibly owing to the active fishery such as fixed shore net fishing, gill net fishing and angling. Meanwhile the condition of fishes represented by the length-weight relationship of fish species did not show the significant differences between two lakes and showed normal condition. To evaluate the impact of physical disturbance such as loud noise and turbulent wave from water recreational activities to fishes precisely, further studies including physiological responses to stress an spawing activity should be needed.

• Mycobiology
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• v.41 no.3
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• pp.121-125
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• 2013

Arbuscular mycorrhizal fungi (AMF) have mutualistic relationships with more than 80% of terrestrial plant species. This symbiotic relationship is ancient and would have had important roles in establishment of plants on land. Despite their abundance and wide range of relationship with plant species, AMF have shown low species diversity. However, molecular studies have suggested that diversity of these fungi may be much higher, and genetic variation of AMF is very high within a species and even within a single spore. Despite low diversity and lack of host specificity, various functions have been associated with plant growth responses to arbuscular mycorrhizal fungal colonization. In addition, different community composition of AMF affects plants differently, and plays a potential role in ecosystem variability and productivity. AMF have high functional diversity because different combinations of host plants and AMF have different effects on the various aspects of symbiosis. Consequently, recent studies have focused on the different functions of AMF according to their genetic resource and their roles in ecosystem functioning. This review summarizes taxonomic, genetic, and functional diversities of AMF and their roles in natural ecosystems.

• Fisheries and Aquatic Sciences
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• v.21 no.3
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• pp.12.1-12.15
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• 2018

A preliminary investigation of length-weight relationship, condition factor and reproductive ecology of African pike fish, Hepsetus odoe, was conducted between June to August 2010 in Lake Eleiyele. A total of 90 samples of the fish species were caught by fishermen using gill nets. The results obtained showed that fish species had isometric growth pattern (contrary to negative allometry expected) with the growth exponent, b value of 2.64, that is not statistically different from 3 and with high statistical power of 0.866 when tested. The mean condition factor, K, was found to be 2.6, indicating that the fish species were in good condition in the study area. For reproductive biology, mean fecundity and gonadosomatic index were found to be 1349 eggs and 3.53% respectively. This study was necessary to fill the knowledge gap and provide information for management and conservation of the investigated species in the lake system. This study recommended that further research needs to be conducted because the sampling was carried out in the rainy season period and within a quarter of a year.

• Journal of Plant Biology
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• v.32 no.3
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• pp.181-188
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• 1989

C-banded karyotypes in A. anisopodium, A. sacculiferum, A. deltoide-fistulosum and A. splendens were investigated. The chromosome compositions were diploid of 2n=16 in A anisopodium and A. splendens, and teiraploid of 2n=32 in a. sacculiferum and A. deltoide-fistulosum. The bands were distributed in telomeric parts of the chromosomes dominantly in addition to interstitial regions sporadically, resulting in the specific C-banded karyo types according to the species. No centromeric band was observed in these species except only one chromosome in A. deltoidefistulosum. The interspecific relationship based on the C-band distribution will be discussed.