• Mycobiology
• /
• v.41 no.4
• /
• pp.191-201
• /
• 2013

Distinguishing individual Russula species has been difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. Due to highly similar macroscopic features, such as the presence of a red-cap, species identification within the Russula subgenus Amoenula is particularly difficult. Three species of the subgenus Amoneula have been reported in Korea. We used a combination of morphology and three molecular markers, the internal transcribed spacer (ITS), 28S nuclear ribosomal large subunit (LSU), and RNA polymerase II gene (RPB2), for identification and study of the genetic diversity of Russula subgenus Amoenula in Korea. We identified only two species in Korea (R. mariae and R. violeipes); these two species were indistinguishable according to morphology and LSU, but were found to be reciprocally monophyletic species using ITS and RPB2. The markers, ITS, LSU, and RPB2, have been tested in the past for use as DNA barcoding markers, and findings of our study suggest that ITS and RPB2 had the best performance for the Russula subgenus Amoneula.

• Korean Journal of Plant Taxonomy
• /
• v.37 no.1
• /
• pp.61-78
• /
• 2007

The general morphological characters of Lysimchia sect. Spicatae were reviewed. From the results, numerical analyses were conducted based on 34 morphological characters from 14 populations of three species in Korea in order to investigate the key characters among the treated taxa and taxonomic delimitation and to discuss the systematic relationship. Habitat, leaf apices, presence or absent of hair on leaves, stipules, stems and bracts, branch of stems, kind of hair on floral axis and pedicels, shape of florescens were important diagnostic characters to identify the species. The relationship between Lysimachia clethoides and L. barystachys was more close than that of L. fortunei based on the quantitative characters. Also, a new key was made up from the result.

• Korean Journal of Plant Resources
• /
• v.25 no.2
• /
• pp.257-270
• /
• 2012

The taxonomic relationship of 3 taxa in $Carex$ sect. $Siderostictae$ were revealed by morphological and molecular studies. The morphological characteristics of Korean $Carex$ siderosticta Hance, which is a type species of sect. $Siderostictae$, was first reviewed in comparison with previous records in order to identify the difference. Then the distribution map of this species was formulated through previous floral literatures and voucher specimens. Although the leaf shape which is represented by length/width ratio showed a great variation depending on habitat condition, the rhizome creeping patterns, the presence of hair on the surface leaf margin and perigynium were shown to be good diagnostic characters to define delimitation of species level. The results from molecular study supported $C.$ $ciliatomarginata$ Nakai should be ranked independently on species level.

• Journal of Species Research
• /
• v.1 no.2
• /
• pp.232-239
• /
• 2012

Taxonomy of umbelliferous taxa Heracleum moellendorffii complex has been unclear in their species delimitation in the far-eastern Asian regions. In both Korea and China Heracleum moellendorffii was adopted for their description while H. sphondylium was chosen to describe Japanese Heracleum. From Genbank accessions, taxa collected from Kamtchatka and Promorskiy, Russia were defined as H. maximum, endemic taxon to North America. In this study, we reviewed the taxonomy of Heracleum moellendorffii complex in Korea and neighboring countries on the basis of molecular phylogenies derived from sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) regions. From three Korean accessions of Heracleum investigated in this study, two types of ITS sequences were obtained; two accessions were related to Chinese H. moellendorffii var. moellendorffii and North American H. maximum without forming a clade while the other one was identical to accession from H. maximum from Primorskiy, Russia. In the other hand, Japanese H. moellendorffii (=H. sphondylium ssp. sphondylium var. nipponicum in the flora of Japan) was closely related to H. maximum accessions from Korea and Russia, not nested within the clade comprising several subspecies of H. sphondylium. In order to delimit species boundaries among putatively closely related Heracleum species in fareastern Asian countries, more samples and much more rapidly evolved DNA regions must be investigated with interpretation of morphological and anatomical features.

• Korean Journal of Plant Taxonomy
• /
• v.40 no.4
• /
• pp.267-273
• /
• 2010

The distribution of three typical warm-temperate evergreen trees, Quercus acuta Thunb., Neolitsea sericea (Blume) Koidz., and Machilus thunbergii Siebold & Zucc., were surveyed on the Korean Peninsula based on field and specimen investigations and the distribution maps of the three species were prepared. The distribution patterns of the species correspond to the south coast floristic region in Korea, which includes the distributional areas of Jeju-do, Isl. Ulleung, the southern coastal areas, and the areas up to the islands around Incheon in the Yellow Sea. The northernmost limit of the distribution of Quercus acuta is Isl. Nap of Incheon in the west, and a new distribution was found at Isl. Ulleung in the East Sea; additionally, the limit of Neolitsea sericea is the Deojoek archipelago of Incheon. The northernmost limit of Machilus thunbergii is Isl. Daecheong of Incheon, which is the highest latitude among those of the three species. This distribution survey of evergreen broad-leaved trees in Korea can be used as basic data for the delimitation of floristic regions and as a bio-indicator of climatic change.

• Korean Journal of Plant Taxonomy
• /
• v.34 no.3
• /
• pp.205-220
• /
• 2004

Numerical analysis based on the 67 morphological characters from 28 populations of 6 species of Korean Rhus sensu lato (Anacardiaceae) was performed for the taxonomic delimitation. Based on the results of PCA with 47 quantitative characters, the sum of contributions for the total variance of three major principal components was 77,9% (PCl 35.2%, PC2 22.5% and PC3 20.2%). The sum of contributions for the total variance of three major principal components were 90,7% (PCl 37.7%, PC2 33.0% and PC3 20.0%) based on the results of PCA with 20 qualitative The characters. Two dimensional plotting from PCA results recognized six distinct species. UPGMA phenogram based on simple matching coefficient method recognized clear taxonomic delimitations among six taxa. On the cluster analysis, qualitative characters were more useful for grouping the species treated. Numerical analysis was very valuable to delimit the Korean taxa of Rhus s.l.

• Korean Journal of Plant Taxonomy
• /
• v.52 no.3
• /
• pp.144-155
• /
• 2022

Daphne kiusiana is an evergreen shrub with dense head-like umbels of white flowers distributed in southern Korea, Japan, China, and Taiwan. Plants in China and Taiwan are recognized as var. atrocaulis by having a dark purple stem, elliptic leaves, and persistent bracts. Recently, plants on Jejudo Island were segregated as a separate species, D. jejudoensis, given their elliptic leaves with an acuminate apex, a long hypanthium and sepals, and a glabrous hypanthium. Morphological variations of three closely related taxa, the D. kiusiana complex, were investigated across the distributional range to clarify the taxonomic delimitation of members of the complex. Twelve characters of the leaf and flower were measured from digitized herbarium specimens using the image analysis program ImageJ and were included in a morphometric analysis, the results of which indicate that the level of variation in the characters is very high. The results of a principal component analysis weakly separated D. jejudoensis from D. kiusiana according to their floral characteristics, such as a longer, glabrous hypanthium, and larger sepals. However, some individuals of D. kiusiana, particularly those from Bigeumdo Island, were included in D. jejudoensis. Recognition of D. kiusiana var. atrocaulis based on the leaf shape was not supported in the analysis, and D. jejudoensis may be recognized as a variety of D. kiusiana. Our morphometric analysis shows that digitized images of herbarium specimens could be useful and an additional method by which to investigate more diverse specimens.

• Korean Journal of Plant Taxonomy
• /
• v.34 no.1
• /
• pp.63-74
• /
• 2004

The genus Suaeda is one of the most popular plants on salt marsh areas in Korean Peninsular. The entities that comprise taxa in Korea exhibit widely overlapping ranges in all morphological attributes. Ramdom amplified polimorphic DNA (RAPD) markers were used to clarify taxonomic delimitation among the Korean taxa of Suaeda and to analyse genetic variations among the populations of S. japoniro in western and southern coastal regions. Six decamer primers amplified a total of 65 scorable bands, of which 61 were polymorphic. In all primers investigated, S. glauro of sect. Schanginia is the most distinctive species, compared with others of sect. Heterosperma. S. japonica, S. maritima, and S. malacosperma, which have been hardly distinguished each other by external morphology, were readily recognized by its specific DNA bands. The characteristic RAPD markers were identified local populations of S. japonica, but this feature was not revealed within population.

• ALGAE
• /
• v.33 no.1
• /
• pp.85-99
• /
• 2018

Crustose calcareous algae (CCA) constitute one of the main reef builders on the Abrolhos Bank, Brazil. Once CCA taxonomy is locally understood, differences in growth-forms may be useful for the delimitation of taxa using characteristics such as the presence or absence of surface protuberances. Here, growth-forms were used to identify and quantify the most common CCA taxa on the shallow reefs (3-10 m) of the Abrolhos Bank to determine possible changes in the CCA community over a period of 10 years, and the ecological significance of CCA to local reefs was interpreted. The CCA assemblages were surveyed from 2006-2015 by using fixed photoquadrats at four sites in the inner (10-20 km from the mainland) and mid-shelf reefs (40-75 km from the mainland). The five most common CCA taxa were Pneophyllum conicum, the Lithophyllum kaiserii / Lithophyllum sp. complex, Melyvonnea erubescens, the Hydrolithon boergesenii / Porolithon onkodes complex and Peyssonelia sp. The overall mean CCA cover on the reefs was 20%. A comparison with a previous monitoring study in the same region indicated that the CCA cover nearly doubled from 2003-2008 to 2006-2015. This study reveals that the coral-killing species P. conicum dominated CCA flora on the shallow Abrolhos reefs in the last decade, and the local specific abundance of CCA slightly fluctuated over time and was species- and site-specific. The information obtained in this study contributes to the understanding of the ecology of the key calcifying components of the Abrolhos reefs and provides a useful baseline for exploring the responses of CCA to future environmental changes.

• Korean Journal of Plant Taxonomy
• /
• v.45 no.2
• /
• pp.183-191
• /
• 2015

The palynological characters of 14 taxa of 4 genera (Cynanchum, Metaplexis, Tylophora, Marsdenia) in Asclepiadoideae were observed and measured using SM and SEM. The orientation and granulate surface patterns of the pollinia were important characters for delimitating the genera. The position of the attachment of the translator arms to the pollinia, considered to be an important character for recognizing the above genera, was an overlapping character among the genera and can therefore be utilized only as taxonomy at the species level. Also, we found for the first time that only Tylophora floribunda has an incised margin of the corpusculum. The quantitative characters of the pollinia, arms and corpusculum were useful keys for the delimitation of the species.