• Title/Summary/Keyword: spatio-temporal analysis

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Distinct Spatio-temporal Expression Patterns of Patatin Promoter-GUS Gene Fusion in Transgenic Potato Microtubers (형질전환 감자 소괴경의 발달단계에 따른 Patatin Promoter-GUS 유전자의 발현 분석)

  • Youm, Jung-Won;Kim, Mi-Sun;Lee, Byoung-Chan;Kang, Won-Jin;Jeon, Jae-Heung;Joung, Hyouk;Kim, Hyun-Soon
    • Journal of Plant Biotechnology
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    • v.30 no.1
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    • pp.13-18
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    • 2003
  • This study was carried out to investigate the expression patterns of foreign gene that controlled by tuber-specific patatin promoter in transgenic potatoes. Potato leaf disc cultured in vitro were transformed by the Agrobacterium strain LBA4404 containing pBl121 or pATGUS from potato cv. Desiree. In order to select the transgenic lines, gene-specific primers deduced from the NPTII were synthesized and used for polymerase chain reaction. The down part of the putative transgenic potatoes was transplanted weekly onto sucrose-enriched medium to accelerate the microtuber formation. RNA gel blot analysis was performed on the total RNAs obtained from tuber that had been harvested at a week interval. Also, histochemical assay was observed in the explants transformed with either pBI121 or pATGUS. Results showed that the transgenic plant containing pATGUS expressed GUS transcripts mainly at the tuber, not in stem, with the highest expression level in 5 weeks-grown microtubers. In contrast to pATGUS plants, the transformed plants with pBI121 showed an equal expression pattern throughout the whole developing stages. Consistent with RNA gel blot analysis, histochemical GUS staining and enzyme activity exhibited pATGUS transcripts were at the highest level in 5 weeks cultures. From these results, we suggest that the best stage to analyze the foreign gene introduced by patatin promoter into potato plants is at 5 weeks cultures after tuber formation.

Site Selection for Geologic Records of Extreme Climate Events based on Environmental Change and Topographic Analyses using Paleo Map for Myeongsanimni Coast, South Korea (고지도 기반 환경변화연구 및 지형분석을 통한 명사십리 해안의 제4기 연안지대 이상기후 퇴적기록 적지선정)

  • Kim, Jieun;Yu, Jaehyung;Yang, Dongyoon
    • Economic and Environmental Geology
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    • v.47 no.6
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    • pp.589-599
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    • 2014
  • This study selected optimal sites in Myeongsasimni located in west coast of Korea for stratigraphic research containing extreme climate event during quaternary period by spatio-temporal analyses of changes in sedimentary environment and land use employing 1918 topographic map, 2000 digital terrain map, 1976 and 2012 air photographies. The study area shows no significant changes in topographic characteristics that hilly areas with relatively large variations in elevation are distributed over north and south part of the study area, and sand dues are developed along the coast line. Moreover, flat low lying areas are located at the back side of the sand dues. The movement of surface run off and sediment loads shows two major trends of inland direction flow from back sides of sand dunes and outland direction flow from high terrains inland, and the two flows merge into the stream located in the center of the study area. Two sink with individual area of $0.2km^2$ are observed in Yongjeong-ri and Jaryong-ri which are located in south central part and south part of the study area, respectively. In addition, sea level change simulation reveals that $3.4km^2$ and $3.64km^2$ are inundated with 3 m of sea level rise in 1918 and 2000, respectively, and it would contribute to chase sea level change records preserved in stratigraphy. The inundated areas overlaps well with sink areas where it indicates the low lying areas located in south cental and south part of the study area are identical for sediment accumulation. The areas with minimal human impact on sediment records over last 100 years are $3.51km^2$ distributed over central and south part of the study area with the land use changes of mud and rice field in 1918 to rice field in 2012. The candidate sites of $0.15km^2$ in central part and $0.09km^2$ in south part are identified for preferable locations of geologic record of extreme climate events during quaternary period based on the overlay analysis of optimal sedimentary environment and land use changes.

Spatio-Temporal Distribution of Benthic Polychaetous Community along the South-eastern Coast of Geoje Is. (거제도 남동부 연안 저서다모류군집의 시.공간적 분포)

  • Lim, Kyeong-Hun;Lee, Jung-Ho;Shin, Hyun-Chool
    • Korean Journal of Environmental Biology
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    • v.24 no.4
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    • pp.392-407
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    • 2006
  • This study was seasonally undertaken to investigate the benthic polychaetous community along the South-eastern coast of Geoje Is., in February 2004 to November. The macrobenthic fauna showed mean density of 953 md. $m^{-2}$ in February, 1,193 ind. $m^{-2}$ in May, 1,130 ind. $m^{-2}$ in September and 991 ind. $m^{-2}$ in November. Major faunal groups are polychaetes, crustaceans and mollusks, corresponding to 81.6%, 7.9%, 7.0% in total mean density during the sampling seasons, respectively. There was significant difference (two-way ANOVA, p<0.001) among stations in densities of polychaetes. But no significant relationship could be found between the densities of polychaetes and sampling seasons (two-way ANOVA, p>0.05). The density and the species number of polychaetes were high in the coastal area near the Jisepo port, independent of sampling seasons. Several indicator species for the organically enriched environments such as Lumbrineris longifolia, Tharyx sp., Heteromastus filiformis showed high densities during all study periods. Also Magelona japonica included in Ecolgical group I was the most abundant in the whole study period except for February. Cluster analysis on the basis of the species composition showed that the study area could be divided into 3 regions. The density of benthic polychaetes in the coastal area near Jisepo was higher than that in the other assemblages of the study area, due to some predominant opportunistic species, such as Lumbrineris longifolia, Tharyx sp. and Heteromastus filiformis. In addition, the two-way ANOVA showed significant differences among the distribution of AMBI (AZTI's Marine Biotic Index) values, both in sampling stations (p<0.001) and seasons (p<0.01). According to the classification proposed by Borja et at. (2000), the study area was slightly disturbed site.

A Phenology Modelling Using MODIS Time Series Data in South Korea (MODIS 시계열 자료(2001~2011) 및 Timesat 알고리즘에 기초한 남한 지역 식물계절 분석)

  • Kim, Nam-Shin;Cho, Yong-Chan;Oh, Seung-Hwan;Kwon, Hye-Jin;Kim, Gyung-Soon
    • Korean Journal of Ecology and Environment
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    • v.47 no.3
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    • pp.186-193
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    • 2014
  • This study aimed to analyze spatio-temporal trends of phenological characteristics in South Korea by using MODIS EVI. For the phenology analysis, we had applied double logistic function to MODIS time-series data. Our results showed that starting date of phenology seems to have a tendency along with latitudinal trends. Starting date of phenology of Jeju Island and Mt. Sobeak went back for 0.38, 0.174 days per year, respectively whereas, Mt. Jiri and Mt. Seolak went forward for 0.32 days, 0.239 days and 0.119 days, respectively. Our results exhibited the fluctuation of plant phonological season rather than the change of phonological timing and season. Starting date of plant phenology by spatial distribution revealed tendency that starting date of mountain area was late, and basin and south foot of mountain was fast. In urban ares such as Seoul metropolitan, Masan, Changwon, Milyang, Daegu and Jeju, the phonological starting date went forward quickly. Pheonoligcal attributes such as starting date and leaf fall in urban areas likely being affected from heat island effect and related warming. Our study expressed that local and regional monitoring on phonological events and changes in Korea would be possible through MODIS data.

Stand-alone Real-time Healthcare Monitoring Driven by Integration of Both Triboelectric and Electro-magnetic Effects (실시간 헬스케어 모니터링의 독립 구동을 위한 접촉대전 발전과 전자기 발전 원리의 융합)

  • Cho, Sumin;Joung, Yoonsu;Kim, Hyeonsu;Park, Minseok;Lee, Donghan;Kam, Dongik;Jang, Sunmin;Ra, Yoonsang;Cha, Kyoung Je;Kim, Hyung Woo;Seo, Kyoung Duck;Choi, Dongwhi
    • Korean Chemical Engineering Research
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    • v.60 no.1
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    • pp.86-92
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    • 2022
  • Recently, the bio-healthcare market is enlarging worldwide due to various reasons such as the COVID-19 pandemic. Among them, biometric measurement and analysis technology are expected to bring about future technological innovation and socio-economic ripple effect. Existing systems require a large-capacity battery to drive signal processing, wireless transmission part, and an operating system in the process. However, due to the limitation of the battery capacity, it causes a spatio-temporal limitation on the use of the device. This limitation can act as a cause for the disconnection of data required for the user's health care monitoring, so it is one of the major obstacles of the health care device. In this study, we report the concept of a standalone healthcare monitoring module, which is based on both triboelectric effects and electromagnetic effects, by converting biomechanical energy into suitable electric energy. The proposed system can be operated independently without an external power source. In particular, the wireless foot pressure measurement monitoring system, which is rationally designed triboelectric sensor (TES), can recognize the user's walking habits through foot pressure measurement. By applying the triboelectric effects to the contact-separation behavior that occurs during walking, an effective foot pressure sensor was made, the performance of the sensor was verified through an electrical output signal according to the pressure, and its dynamic behavior is measured through a signal processing circuit using a capacitor. In addition, the biomechanical energy dissipated during walking is harvested as electrical energy by using the electromagnetic induction effect to be used as a power source for wireless transmission and signal processing. Therefore, the proposed system has a great potential to reduce the inconvenience of charging caused by limited battery capacity and to overcome the problem of data disconnection.

Macrobenthic Community Structure Along the Environmental Gradients of Ulsan Bay, Korea (울산만의 저서환경 구배에 따른 저서동물군집 구조)

  • Yoon, Sang-Pil;Jung, Rae-Hong;Kim, Youn-Jung;Kim, Seong-Gil;Choi, Min-Kyu;Lee, Won-Chan;Oh, Hyun-Taik;Hong, Sok-Jin
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.14 no.2
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    • pp.102-117
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    • 2009
  • This study was carried out to investigate the extent to which benthic environment of Ulsan Bay was disturbed by organic materials and trace metals from the megacity and industrial complex. Field survey for benthic environment and macroinvertebrate community was seasonally conducted from February to November 2006 at nine stations covering the inside and outside of the bay. TOC was on average 1.7% while four (As, Cu, Pb, Zn) out of seven trace metals measured exceeded the Effects Range Low (ERL) in most of the stations. Total number of species sampled was 199 and mean density was 4,578 ind./$m^2$, both of which were greatly dominated by the polychaetes. Dominant species were Aphelochaeta monilaris (22.6%), Ruditapes philippinarum (17.1%), Magelona japonica (12.2%), Lumbrineris longifolia (9.9%) and their distribution was ruled by the difference in the benthic environmental condition of each station. From the multivariate analyses, four stational groups were identified: northern part of the bay, middle and lower part of the bay, the intersection of Taewha River and Gosa stream and outside of the bay. As a result, the community heterogeneity of inner bay was much more greater than that of outer bay. SIMPER analysis showed that four groups were represented by R. philippinarum-Capitella capitata, A. monilaris-Balanoglossus carnosus, Sinocorophium sinensis-Cyathura higoensis and M. japonica-Ampharete arctica, respectively. Spatio-temporal changes of macroinvertebrate communities in Ulsan Bay were closely related to those of depth, mean grain size and organic content, and Zn was also a meaningful factor in that context.

High-resolution medium-range streamflow prediction using distributed hydrological model WRF-Hydro and numerical weather forecast GDAPS (분포형 수문모형 WRF-Hydro와 기상수치예보모형 GDAPS를 활용한 고해상도 중기 유량 예측)

  • Kim, Sohyun;Kim, Bomi;Lee, Garim;Lee, Yaewon;Noh, Seong Jin
    • Journal of Korea Water Resources Association
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    • v.57 no.5
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    • pp.333-346
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    • 2024
  • High-resolution medium-range streamflow prediction is crucial for sustainable water quality and aquatic ecosystem management. For reliable medium-range streamflow predictions, it is necessary to understand the characteristics of forcings and to effectively utilize weather forecast data with low spatio-temporal resolutions. In this study, we presented a comparative analysis of medium-range streamflow predictions using the distributed hydrological model, WRF-Hydro, and the numerical weather forecast Global Data Assimilation and Prediction System (GDAPS) in the Geumho River basin, Korea. Multiple forcings, ground observations (AWS&ASOS), numerical weather forecast (GDAPS), and Global Land Data Assimilation System (GLDAS), were ingested to investigate the performance of streamflow predictions with highresolution WRF-Hydro configuration. In terms of the mean areal accumulated rainfall, GDAPS was overestimated by 36% to 234%, and GLDAS reanalysis data were overestimated by 80% to 153% compared to AWS&ASOS. The performance of streamflow predictions using AWS&ASOS resulted in KGE and NSE values of 0.6 or higher at the Kangchang station. Meanwhile, GDAPS-based streamflow predictions showed high variability, with KGE values ranging from 0.871 to -0.131 depending on the rainfall events. Although the peak flow error of GDAPS was larger or similar to that of GLDAS, the peak flow timing error of GDAPS was smaller than that of GLDAS. The average timing errors of AWS&ASOS, GDAPS, and GLDAS were 3.7 hours, 8.4 hours, and 70.1 hours, respectively. Medium-range streamflow predictions using GDAPS and high-resolution WRF-Hydro may provide useful information for water resources management especially in terms of occurrence and timing of peak flow albeit high uncertainty in flood magnitude.