• Korean Journal of Microbiology
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• v.42 no.2
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• pp.153-155
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• 2006

We constructed the null mutants of fission yeast Schizosaccharomyces pombe spSac3 gene that is homologous to budding yeast Saccharomyces cerevisiae SAC3 involved in mRNA export out of nucleus. Tetrad analysis showed that the spSac3 is essential for vegetative growth. The spSac3 mutants harboring pREP81X-spSac3 plasmid showed poly(A)+ RNA export defect in the presence of thiamine. These results suggest that spSac3 is also involved in mRNA export from the nucleus.

• Journal of Welding and Joining
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• v.26 no.5
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• pp.43-48
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• 2008

Characteristics of Sn-1.7%Bi-0.7%Cu-0.6%In (hereafter, SBIC) lead-free solder was investigated in this study. The results from SBIC were compared to other lead-free solders such as Sn-3.5%Ag-0.7%Cu (hereafter, SAC), Sn-0.7%Cu (hereafter, SC), and lead-bearing Sn-37%Pb (hereafter, SP) alloy. Tensile properties of bulk solder, wettability, spreading index, bridge and dross were evaluated. As experimental results, tensile strength and elongation of SBIC was 62.5MPa and 21.5%, respectively. The tensile strength was comparable to that of SP solder. The wetting time of SBIC was 1.2 sec at $250^{\circ}C$, and its wetting properties including wetting force were as good as the SAC alloy. However, wettability of the SC was not so good as the SBIC and SAC. The spreading index of SBIC at $250^{\circ}C$ was 71 %, and it was similar level to those of SAC and SC solders. Bridging was not found for all solders of SBIC, SAC and SC in the range from 240 to $260^{\circ}C$. In dross test at $250^{\circ}C$ for an hour, the amount of dross produced from SBIC was about 57% compared to that from SAC.

• Korean Journal of Microbiology
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• v.31 no.2
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• pp.123-128
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• 1993

Genes for dinitrogenase reductase (nifH) and dinitogenase a subunit (nifD) were found to be located on 7.9 kb of EcoRI, 6.5 kb of Sail, 7.3 kb of HindlII and 4.4 kb of Pstl fragments of the genomic blot of Rhizobium sp. SNU003. a symbiotic strain from root nodule of Canavalia lineata. Nine recombinant phage nif-clones were selected from the genomic library constructed by using EMBL-3 BamHI arms of bacteriophage lambda. Among them. Rnif-6 had insert DNA of 15.3 kb. in which 7.6 kb of BamHI!SacI fragment contained nifHD region. Therefore, the 7.6 kb fragment was subcloned into pUC19 and partial restriction map was constructed. As the results, nifH and nifD were found to be located continuously on 4.5 kb of BamHI/BglIl in the genome of Rhizobium sp. SNU003 strain.

• Korean Journal of Veterinary Service
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• v.22 no.2
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• pp.113-119
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• 1999

The most common cause of death is ostrich chick fading syndrome(OCFS), which is due to bacterial infection during artificial incubation and hatching. Six farmed ostrich chicks aged 3 and 10 days in Chonbuk province, were submitted to Chonbuk Livestock Development and Research Institute for necropsy, Clinically, birds showed hair loss, ocular exudate, lethargy, diarrhea, and subsequently died 3-5 days after onset of clinical signs. Grossly, umbilicus was enlarged. White-yellowish purulent nodules were scattered on the lung and the membrane of air-sac was thickened and had inflamed exudate on the surface in two chicks that died 3 days after hatching. In 10 days-old chick, intestine was shown rodding segmentally. Yolk sac was still retarded and its surface was partially hemorrahgic. The synovial fluid of the leg was yellowish. Microscopically, multifocal purulent exudates were scattered on the lung. Capillary microthrombi in the glomerulus were prominent and tubular epithelia were necrotic. Necrotic hepatocytes were scattered and intestine were congested. Microbiologically, Pseudomonas sp and/or E coli were isolated from air-sac, lung and/or liver. This case suggests that poor hygiene during artificial incubation, hatching or in the first week after hatching may cause high mortality of the ostrich chicks.

• The Korean Journal of Soil Zoology
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• v.7 no.1_2
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• pp.51-57
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• 2002

Two new and two known species of mononchid nematodes from Korea are described and illustrated. lotonchus cucumis sp. n. is characterized by 2.9-3.2 ㎜ long body, presence of 3 each pre- and post-vulval papillae, vagina with cuticularized pieces, a long tail with terminal spinneret. Mylonchulus unicus sp. n. has 1.1-1.2 ㎜ long body, and is characterized by having 2 pairs of teeth on subventral walls of buccal cavity; submedian denticles arranged in 2-3 rows, very short post-vulval sac and terminal spinneret. Mononchus sinensis Soni and Nama (1983) and Mononchus aquaticus Coetzee (1968) are reported for the first time from Korea.

• Parasites, Hosts and Diseases
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• v.26 no.2
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• pp.127-132
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• 1988

Two adult specimens of heterophyid cuke, which belong to the genus Stictodora, were collected from the stool of a 24-year old man after chemotherapy. The nukes were morphologically characterized by their small body siRe (0.90~0.98mm long and 0.38 mm wide) and peculiar structure of ventrogenital sac with armed gonotyl (with about 12~I5 spines) not enveloping genital pore. Species identification is deferred until more worms are obtained, although they closely resemble Stictadora fuscatum (Onji and Nishio, 1916). The Patient used to eat raw flesh of mullets and gobies, which are regarded as the infection source of Stictodora sp., together with 3 other kinds of heterophyids described elsewhere. This is the first record of human Stictodora infection in the literature.

• Parasites, Hosts and Diseases
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• v.33 no.3
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• pp.147-154
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• 1995

A new haplosplanchnid cercaria, Cercaria kuwaitae VI sp. n., was found in the prosobranch snail Cerithidea cincingulata in the Kuwait Bay Details are presented on the morphology and behavior of the cercaria and the encystment process. The new cercaria is a biocellate, distome, with a prominent single sac-like intestinal cecum extending well posterior to the ventral sucker and develops in simple sporocysts. It differs from known haplosplanchnid cercariae in the absence of finger-like processes on the tail, and the presence of V-shaped excretory vesicle extending beyond ventral sucker and the presence of cervical glands. The surface topography of the cercaria and its sporocyst is examined by scanning electron microscopy. This is the first haplosplanchnid cercaria to be described from a Cerithidea species.

• Journal of Veterinary Clinics
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• v.18 no.2
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• pp.174-177
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• 2001

A nine-month-old male ostrich (Struthio camelus) exhibited loss of appetite and dyspnea for 10 days, followed by emaciation and death. Grossly, multiple white nodules measuring 1-3 mm in diameter were observed in the surface of and inside of the lung and in the mucosa of the air sac. Microscopically, the granuloma formations were observed in the lung and air sac. The core of granuloma consisted mainly of macrophages and fibroblasts. The thin layer comprising the giant cells and macrophages surrounded the granuloma. By Periodic acid Schiff reaction, mycelia were detected especially in the core of granuloma. From the present results, the causative agent inducing the death of an ostrich was thought to be Aspergillus sp. This is the first report of the occurrence of acute Aspergillus pneumonia (brooder pneumonia) in an adult male ostrich in Korea.

• Journal of Species Research
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• v.13 no.3
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• pp.340-351
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• 2024

A new species of the canuellid genus Brianola Monard, 1926 (Copepoda: Harpacticoida) was collected during a meiofauna survey in July 2021 at the intertidal muddy sand of Phuket Island, Thailand. Currently, there are only eight known species of Brianola: B. stebleri, B. exigua, B. curvirostris, B. elegans, B. sydneyensis, B. vangoethemi, B. hamondi, and B. haliensis. Thus, the Brianola rawaiensis sp. nov. from Thailand is the ninth species of the genus. The new species, Brianola rawaiensis is most closely related to B. haliensis by sharing a four-segmented antennule in females and five-segmented antennule in males, eight-segmented antennary exopod and three-segmented antennary endopod, and P1 exp-3 with 5 spines/setae. However, B. rawaiensis sp. nov. is different from B. haliensis by having: 1) P1 exp-1 with 1 outer pinnate spine, 2) P1 exp-2 with inner plumose seta, 3) P2 exp-3 with 3 setae/spines, 4) P2 enp-1 with one inner plumose seta, 5) P4 enp-1 with inner seta. Sexual dimorphism is expressed in the antennule and segmentation of the urosome. Ovigerous female bearing one egg sac with 20 eggs.

• Korean Journal of Microbiology
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• v.30 no.4
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• pp.246-251
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• 1992

Molccular cloning of nod genes from Bradvrhizobium sp. SNU001, a nitrogen-fixing symbiont isolated from thc root nodules of soybean (Clycine trim) . was carried out. nod genes were found to be located on thc genome of the symbiont by gcnomic hybridization with 4.5 kb EcoRI/HndIII fragment (nod DABC) of Rhizohium meliloti as probe. Genomic library of this symbiont was constructed using h phage EMBL3-BanlHI vector. from which five nod positive clones were sclectcd by primary and secondary screening methods. The partial restriction map of inserted genomic DNA of h CNS-l(c1one 2) was constructed. and 3.9 kh Bun7HI fragment. which showed strong hybridization signal to the probe, was subcloned into pBS KS(+) plasmid vector. Partial restriction inap ot' a selected subclone (pBjCNS-I) was constructed and nod DABC was found to be located on the 1.8 kb KpnI/Sacl fragment of this subclone.