• The Plant Pathology Journal
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• v.32 no.4
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• pp.371-376
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• 2016

Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the reporter gene, green fluorescence protein (GFP), but the expression of GFP was not detected due to the necrotic cell death on the infiltrated leaves. The p19 silencing suppressor of GALV was engineered to inactivate its expression and GFP was successfully expressed with unrelated silencing suppressor, HC-Pro, from soybean mosaic virus. The pGMG vector was used to knock down magnesium chelatase (ChlH) gene in N. benthamaina and the silencing phenotype was clearly observed on systemic leaves. Altogether, the GALV-derived vector is expected to be an attractive tool for useful gene expression and VIGS vectors in grapevine as well as N. benthamiana.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.04a
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• pp.50.2-50
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• 1999

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.334-334
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• 2005

• Korean journal of applied entomology
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• v.29 no.3
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• pp.184-189
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• 1990

A nuclear polyhedrosis virus (NPV) of the tobacco cutworm, Spodoptera litura would be a promisible agent for the control of the insect. To develop a viral insecticide using S. litura NPV, effect of spray on soybean leaves, temperature, storage, an sunlight on the pathogenicity of the virus were studies as follows: Median lethal concentration ($LC_{50}$) of the virus sprayed on the leaves against the third and the fifth instar larvae were $1.301\times10^{4 PIBS}/ml$ and $1.087\times10^{5 PIBS}/ml$, respectively. On the concentration of $1.0\times10^{5 PIBS}/ml$, median lethal times ($LT_{50}$) were 7.3 days for the 3rd and 8.9 days for the 5th instar larvae. Stability of S. litura NPV was quickly decreased at the higher temperate than $60^{\circ}C$ and at the longer exposure to the higher temperature. Storage of the virus at $-20^{\circ}C$ was kept higher pathogenicity than $4^{\circ}C$ and $25^{\circ}C$. Viral activity was maintained more than 10 days in the sprayed-under leaves, but decreased at 3 day after spray in th sprayed-on the leaf surface when exposed the virus to sunlight.

• Korean journal of applied entomology
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• v.21 no.2 s.51
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• pp.53-60
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• 1982

Isolates of soybean mosaic virus (SMV) strains classified based on virulence in silt resistant soybean cultivars caused the same reactions in soybean cultivars used as differentials as those obtained by sap inoculations to the same cultivars. Five species of aphids (Myzus persicae SULZ., Aphis craccivora KOCH, Aphis citricola VAN., Rhopalosiphum maidis FIT., End R. padi L.) were able to transmit each of SMV strains. However, R. maidis and R. padi were inefficient vectors for transmission of SMV strain G3. Spread if four SMV strains (G2, G3, G6, and G7) was monitored in the field from sapinoculated plants in a one meter row of Williams soybeans (source plants) to plants in an adjacent row of Williams 80cm away (test plants). Test plants wert downwind from the source plants. A complete block design was used. Spread of strain G6 was significantly greater than that of other three strains. Two hundred six aphids were collected from June 27, 1979 to August 2, 1979 in the same field. A. citricola was the mist prevalent, comprising $68\%$ of the total aphids. Yields of Williams inoculated with each strain were also compared. Yields were the least from plants inoculated with strain G2 following G6, G3, and G7 in that order.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.60-60
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• 2005

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.04a
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• pp.48.2-48
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• 1999

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.224.1-224
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• 2000

No Abstract, See Full Text

• Applied Biological Chemistry
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• v.38 no.3
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• pp.224-231
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• 1995

To increase the expression of a foreign protein in transgenic plant, the benefits of 5'-untranslated leader sequences of tobacco mosaic virus (TMV) RNA or soybean glycinin gene, Gy2, fused to a protein coding sequence were exploited. pGA643-derived plasmid contains 355 promoter of cauliflower mosaic virus, protein coding sequence of maize 10 kDa zein (10kZ) and Gy2 terminator. The leader from Gy2 or TMV RNA was inserted between the promoter and the coding sequence in each construct. The recombinant DNAs were introduced into tobacco plants by Agrobacterium mediated leaf disc transformation method. Although the transgene without the leader had more transcripts than the others, mRNAs containing the leader were translated more efficiently. It might be due to difference in the length of 5'-untranslated sequence and context surrounding the AUG codon, but could be sequence specific rather. These results suggest that the leader sequences of Gy2 and TMV play important roles as an enhancer in translational control of foreign gene in transgenic tobacco plant.

• Korean journal of applied entomology
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• v.29 no.4
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• pp.252-256
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• 1990

Viral insecticides were formulated with Spodoptera litura nuclear polyhedrosis virus and different U.V. protectants based on white carbon, molasses, and white carbon and molasses mixture to use as microbial control agents. Effect of rainfall on the attachment of formulated viruses to leaves was no different between the treated and the non-treated experiment. Persistence of the formulations was lated 5 days on the surface-sprayed leaves and 12 days on the under-sprayed leaves which was showing 60% mortality. Total mortality of the viral insecticides was more than 97% with no differences among them. Field evaluation of three viral insecticides in soybean field was very successful then carried out in Chinju, a southern part of Korea. Mortality by the formulation in the field during 14 days was more than 93%, but the formulations contained molasses showed phytotoxicity on soybean leaves. Spray effect of the viral insecticides was begun to appear from 7 days later than that of chemical insecticide.