• Title/Summary/Keyword: soybean seed

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Inheritance of 7S α' - subunit Protein in Soybean Seed (콩의 7S α' - subunit 단백질의 유전)

  • Sung, Mi-Kyung;Kim, Kyung-Roc;Park, Jung-Soo;Hwang, Kyo-Jin;Chung, Jong-Il
    • Journal of agriculture & life science
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    • v.43 no.5
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    • pp.39-42
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    • 2009

  • Soybean is an important sources of plant proteins for human and animal nutrition. The use of soybean proteins has been expanded in the food industry due to their excellent nutritional benefits. But, Soybeans contain allergenic proteins that cause allergies to sensitive individuals. ${\beta}$-conglycinin(7S globulin) and glycinin(11S globulin) are the major components of storage protein in soybean. ${\beta}$-conglycinin consists of three subunits, ${\alpha}^{\prime}$, ${\alpha}$, ${\beta}$ and exhibits poorer nutritional and food processing properties than glycinin. There is a great deal of interest in the development of soybean lines with reduced amounts of ${\beta}$-conglycinin. The objective of this study was to determine the inheritance of ${\alpha}^{\prime}$-subunit protein in 7S globulin. F2 population was developed from the cross of "Jinpumkong2ho"(${\alpha}^{\prime}$-subunit presence) and PI506876(${\alpha}^{\prime}$-subunit absence) parent. Total 98 of F2 seeds were obtained and analyzed for the segregation of ${\alpha}^{\prime}$-subunit protein by SDS-PAGE. Among 98 F2 seeds, 70 F2 seeds showed ${\alpha}^{\prime}$-subunit protein and 28 F2 seeds did not show ${\alpha}^{\prime}$-subunit protein. The segregation ratios of 3 : 1 for presence and absence of ${\alpha}^{\prime}$-subunit protein were observed(${\chi}^2=0.667$, P=0.414). These data indicate that presence and absence of ${\alpha}^{\prime}$-subunit protein is controlled by a single major gene and might be useful for strain selection of 7S protein reduced soybean.

Expression and purification of Soybean $\beta$-Conglycinin from (콩 $\beta$-Conglycinin의 대장균 발현과 정제)

  • 노영희
    • The Korean Journal of Food And Nutrition
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    • v.12 no.2
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    • pp.184-190
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    • 1999

  • Soybean protein consists of two major components $\beta$-conglycinin and glycinin which together consti-tute 70% of the total seed storage protein at maturity. $\beta$-Conglycinin is trimeric glycoprotein and for-med by the assembly of various combinations of three subunits $\alpha$,$\alpha$' and $\beta$ which have molecular weig-hts of 69,000, 72,000 and 42,000, respectively. Recently $\beta$-conglycinin was identified as powerful LDL lip-oprotein receptor activation hypercholesterolemia and major allergenic proteins. To investigate these reasons we constructed an expression system of cDNA encoding $\alpha$-subunit of $\beta$-conglycinin in Escherichia coli and purified the expressed protein. The pro-$\beta$-conglycinin synthesized in Escherichia coli BL 21 (DE3)comprised approximately 15% of the total bacterial proteins and the expressed protein are formed sol-uble and trimer such as native protein in Escherichia coli cells. The highly expressed protein was purified to homogeneity by salt precipitation with 20~40 % ammonium sulfate ion-exchange chromatography with Q-sepharose and hydrophobic column chromatography with Butyltoyopearl.

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Patterns between wall pressures and stresses with grain moisture on cylindrical silo

  • Kibar, Hakan
    • Structural Engineering and Mechanics
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    • v.62 no.4
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    • pp.487-496
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    • 2017

  • The focus of this study were to investigate patterns between wall pressures and stresses with grain moisture of soybean and rice varieties widespread cultivated in Turkey in order to determine needed designing parameters for structure analysis in silos at filling and discharge. In this study, the wall pressures and stresses were evaluated as a function of moisture contents in the range of 8-14% and 10-14% d.b. The pressures and von Mises stresses affected as significant by the change of grain moisture content. The main cause of pressure and stress drops is changed in bulk density. Therefore is extremely important bulk density and moisture content of the product at the structural design of the silos. 4 mm wall thickness, were determined to be safe for von Mises stresses in both soybean and rice silos is smaller than 188000 kPa.

  • https://doi.org/10.12989/sem.2017.62.4.487 인용 KSCI

Regulation Mechanism of Soybean Storage Protein Gene Expression (대두 저장단백질 유전자의 발현 조절 메카니즘)

  • 최양도;김정호
    • Proceedings of the Botanical Society of Korea Conference
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    • 1987.07a
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    • pp.283-307
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    • 1987

  • Glycinin and $\beta$-conglycinin are the most abundant storage protein in soybean. These proteins are known to be synthesized predominantly during germination and cell expansion phase of seed development for short period, and synthesized not in other tissues. Genes encoding these storage proteins are useful system to study the mechanism of development stage and tissue specific gene expression in eukaryotes, especially plants, at the molecular level. The cDNA and genomic clones coding for glycinin have been isolated and regulation mechanism of the gene expression has been studied. Initially, development and tissue-specific expression of the glycinin gene is regulated at the level of transcription. Post-transcriptional processing is also responsible for delayed accumulation of the mRNA. Translational control of the storage protein gene has not been reported. Post-translational modification is another strategic point to regulate the expression of the gene. It is possible to identify positive and/or negative reguratory clements in vivo by producing transgenic plants agter gene manipulation. Elucidation of activation and repression mechanism of soybean storage protein genes will contribute to the understanding of the other plant and eukaryotic genes at molecular level.

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