Inheritance of 7S α' - subunit Protein in Soybean Seed

콩의 7S α' - subunit 단백질의 유전

  • Sung, Mi-Kyung (Dep. of Agronomy(Insti. of Agric. and Life Sci.), Gyeongsang Nat'l Univ.) ;
  • Kim, Kyung-Roc (Dep. of Agronomy(Insti. of Agric. and Life Sci.), Gyeongsang Nat'l Univ.) ;
  • Park, Jung-Soo (Dep. of Agronomy(Insti. of Agric. and Life Sci.), Gyeongsang Nat'l Univ.) ;
  • Hwang, Kyo-Jin (Dep. of Agronomy(Insti. of Agric. and Life Sci.), Gyeongsang Nat'l Univ.) ;
  • Chung, Jong-Il (Dep. of Agronomy(Insti. of Agric. and Life Sci.), Gyeongsang Nat'l Univ.)
  • 성미경 (경상대학교 농학과(농업생명과학연구원)) ;
  • 김경록 (경상대학교 농학과(농업생명과학연구원)) ;
  • 박정수 (경상대학교 농학과(농업생명과학연구원)) ;
  • 황교진 (경상대학교 농학과(농업생명과학연구원)) ;
  • 정종일 (경상대학교 농학과(농업생명과학연구원))
  • Received : 2009.09.04
  • Accepted : 2009.10.24
  • Published : 2009.10.30

Abstract

Soybean is an important sources of plant proteins for human and animal nutrition. The use of soybean proteins has been expanded in the food industry due to their excellent nutritional benefits. But, Soybeans contain allergenic proteins that cause allergies to sensitive individuals. ${\beta}$-conglycinin(7S globulin) and glycinin(11S globulin) are the major components of storage protein in soybean. ${\beta}$-conglycinin consists of three subunits, ${\alpha}^{\prime}$, ${\alpha}$, ${\beta}$ and exhibits poorer nutritional and food processing properties than glycinin. There is a great deal of interest in the development of soybean lines with reduced amounts of ${\beta}$-conglycinin. The objective of this study was to determine the inheritance of ${\alpha}^{\prime}$-subunit protein in 7S globulin. F2 population was developed from the cross of "Jinpumkong2ho"(${\alpha}^{\prime}$-subunit presence) and PI506876(${\alpha}^{\prime}$-subunit absence) parent. Total 98 of F2 seeds were obtained and analyzed for the segregation of ${\alpha}^{\prime}$-subunit protein by SDS-PAGE. Among 98 F2 seeds, 70 F2 seeds showed ${\alpha}^{\prime}$-subunit protein and 28 F2 seeds did not show ${\alpha}^{\prime}$-subunit protein. The segregation ratios of 3 : 1 for presence and absence of ${\alpha}^{\prime}$-subunit protein were observed(${\chi}^2=0.667$, P=0.414). These data indicate that presence and absence of ${\alpha}^{\prime}$-subunit protein is controlled by a single major gene and might be useful for strain selection of 7S protein reduced soybean.

인간과 가축의 영양을 위한 식물성 단백질의 주요 공급원은 콩이며 콩 단백질은 영양 및 기능성면에서 우수하여 소비가 점차 증가하고 있다. 그러나 콩 단백질에는 알러지를 일으키고 영양가치를 떨어뜨리는 성분도 포함되어져 있다. 7S 및 11S 글로블린은 콩 저장단백질의 대부분을 차지하며 7S는 영양가치가 떨어지고 7S의 함량을 줄어든 콩 계통 육성에 대한 관심이 높아지고 있다. 7S 성분중의 하나인 ${\alpha}^{\prime}$-subunit의 유전양상을 파악하기 위하여 진품콩2호와 PI506876의 교배로부터 98개의 F2 종자가 얻어졌다. SDS-PAGE로 각각의 종자를 분석한 결과 ${\alpha}^{\prime}$-subunit을 가진 종자가 70개였고 결핍된 종자가 28개였다. 이러한 유전양상은 단인자 유전원칙 (${\chi}^2=0.667$, P=0.414)과 일치하여 콩 종자에서 7S의 ${\alpha}^{\prime}$-subunit 단백질은 한 개의 유전자에 의 해서 좌우되었다. 이 결과는 7S 단백질 함량이 줄어든 콩 계통 선발에 유용하게 활용될 것으로 기대된다.

Keywords

References

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