• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.3
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• pp.198-204
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• 2021

Intercropping cereals with legumes is known to improve forage production and crude protein yield. Sorghum × sudangrass hybrids (SSH) have excellent dry matter content and high cultivation temperatures. In this study, we investigated the growth characteristics, forage productivity, and feed value of intercropping SSH with different legumes in rice paddy fields. We used five treatments in this study SSH monocropping and four intercropping treatments of SSH with, lablab, cowpea, sesbania, and two cultivars of soybean (Chookdu 1 and 2). SSH plant height was not significantly different between the monocropping and intercropping treatments. However, the plant heights of lablab, cowpea, and sesbania were significantly higher than those of the two soybean cultivars. The total dry matter yield (kg/ha) was significantly higher in SSH monocropping than in intercropping; among the intercropping treatments, the one with SSH and Chookdu 2 yielded the highest total dry matter yield. The SSH feed value was significantly different between the monocropping and intercropping treatments, although there were no differences between the intercropping treatments. Among the intercropped legumes, lablab showed the highest neutral detergent fiber and acid detergent fiber contents, and cowpea had the highest crude protein content. These results reveal that intercropping SSH with legumes in paddy fields could be a promising cultivation technique to maintain stable forage productivity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.5
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• pp.872-877
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• 1997

Soybean curd whey(SCW) containing plenty of nutrients is the discarded by-product in soybean curd processing. To test the potential utilization of SCW as a medium for the cultivation of Lactobacilus acidophilus, the chemical composition of SCW, as well as the growth, acid production, acid-tolerance, and bile-tolerance of L. acidophilus in SCW-based media were investigated. Sucrose and stachyose, the main free sugars of SCW, were 0.42% and 0.41%, respectively. SCW contained 36.1mg/L of total free amino acids. L. acidophilus KFRI 150 showed lower cell growth and acid production in SCW than those in MRS broth. In optimized SCW-based medium supplemented with 1.0% glucose, 0.5% yeast extract, and 0.2% $K_{2}HPO_{4}$, the growth and acid production of L. acidophilus KFRI 150 increased by twice against those in SCW. In optimized SCW-based medium, the viable counts of four L. acidophilus strains were mostly at the level of $10^{9}$/ml, which is similar to those in MRS broth. Each acid-tolerance and biletolerance of four L. acidophilus strains cultured in optimized SCW-based medium and MRS broth showed no dist-inguishable difference.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.437-447
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• 2012

Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plant-growth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered into 2 groups with 75% similarity and 13 subgroups with 85% similarity, suggesting that the genetic distance correlated with the source of isolation. The isolates were also analyzed for certain growth-promoting activities. Auxin synthesis was widely distributed among the isolates, with values ranging from 93.34 to 1653.37 ${\mu}M$ auxin per ${\mu}g$ of protein. The phosphate solubilization index ranged from 1.25 to 3.89, and siderophore index varied from 1.15 to 5.25. From a total of 57 isolates, 3 showed an ability to biologically fix atmospheric nitrogen, and 7 showed antagonism against the pathogen Sclerotinia sclerotiorum. The results of biochemical characterization allowed identification of potential candidates for the development of biofertilizers targeted to the sunflower crop.

• Korean Journal of Food Science and Technology
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• v.13 no.3
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• pp.247-252
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• 1981

As a primary study for the improvement of yield and preference of soybean sprouts, the water absorption rates by soaking and the growing characteristics of sprouts were investigated. When the soybeans were soaked in subterranean water at $18^{\circ}C$ for 4 hours, water absorption rates by weight percent of soybeans were ranged $167.0{\sim}194.5%$, and it means that the absorption rates do not greatly influence its germination rates. The yield of soybean sprout whose common name is Guryetai and of the sprout treated with 1 ppm Gibberellin marked the highest value, 837% and 585%, respectively. In case of industrial cultivation of soybean sprout, it is supposed that sprinkling with subterranean water is good for prevention of deterioration by growth of microorganisms and respiratory heat.

• Korean Journal of Food Science and Technology
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• v.33 no.1
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• pp.84-88
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• 2001

About 100 bacterial strains producing proteolytic enzymes were isolated from Korean traditional soy paste Doenjang. Among them, strain SYG3 producing the highest level of angiotensin converting enzyme (ACE) inhibitor into the culture medium was selected and identified as Bacillus pumilus according to the Bergey's mannual of systematic bacteriology. Soybean powder as a nitrogen source and glucose as a carbon source supported high level of ACE inhibitor production. The presence of 3% NaCl also enhanced the production of ACE inhibitor in the medium. The optimum initial pH of the medium and culture temperature for the production of ACE inhibitor were 7.0 and $32^{\circ}C$, respectively. The maximal level of ACE inhibitory effect was obtained after 36 hours of cultivation under the optimized conditions, which was about 98% of inhibition ratio.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.22-31
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• 2005

To evaluate growth adaptation of the soybean in paddy field, five soybean cultivars were grown in Yeoncheon with two cultivating methods, level row and high ridge. Growth characters of the top plants, such as stem lengths, numbers of branches, diameters of stem, were higher under high ridge than under level row. However, the differences among the cultivars were bigger than those between the cultivation methods in each cultivar. Comparing the growth of top plants, dry matters in level row were higher than those in high ridge in V5 stage, however, via verses as growth progressed with significant differences among the cultivars. Roots were more developed under high ridge than those under level row during whole growth stages, such as V5, R2, and R5 stages. T/R ratio in level row was significantly higher than that in high ridge with significant differences among the cultivars.

• Molecules and Cells
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• v.23 no.3
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• pp.280-286
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• 2007

L-asparaginase (EC 3.5.1.1) catalyzes the hydrolysis of the amide group of L-asparagine, releasing aspartate and $NH_4{^+}$. We isolated a low temperature-inducible cDNA sequence encoding L-asparaginase from soybean leaves. The full-length L-asparaginase cDNA, designated GmASP1, contains an open reading frame of 1,258 bp coding for a protein of 326 amino acids. Genomic DNA blotting and fluorescence in situ hybridization showed that the soybean genome has two copies of GmASP1. GmASP1 mRNA was induced by low temperature, ABA and NaCl, but not by heat shock or drought stress. E. coli cells expressing recombinant GmASP1 had 3-fold increased L-asparaginase activity. A possible function of L-asparaginase in the early response to low temperature stress is discussed.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.4
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• pp.55-64
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• 2021

The expansion of upland crop cultivation in rice paddy fields is recommended by the Korean government to solve the problem of falling rice price and reduction of rice farmer's income due to oversupply of rice. However, water use efficiency is significantly influenced by the land use change from paddy field to upland. Therefore, this study aimed to evaluate the water budget of soybean grown in using APEX (Agricultural Policy and Environmental eXtender) model. The amount of runoff was measured in a test bed located in Iksan, Jeollabu-do and used to calibrate and validate the simulated runoff by APEX model. From 2019 to 2020, the water budget of soybean grown in uplands were estimated and compared with the one grown in paddy fields. The calibration result of AP EX model for runoff showed that R² (Coefficient of determination) and NSE (Nash-Sutcliffe efficiency) were 0.90 and 0.89, respectively. In addition, the validated results of R² and NSE were 0.81 and 0.62, respectively. The comparative study of each component in water budget showed that the amounts of evapotranspiration and percolation estimated by APEX model were 549.1 mm and 375.8mm, respectively. The direct runoff amount from upland was 390.1 mm, which was less than that from paddy fields. The average amount of irrigation water was 28.7 mm, which was very small compared to the one from paddy fields.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.3
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• pp.248-256
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• 2018

Reduction in the occurrence of weed and erosion of slope soil by living mulch barley simultaneously sown with soybean were investigated at the Upland Experimental Field in Suwon City in 2013 and 2014. The aboveground dry matter of 200-300, 100-200 kg/10a of living mulch barley was obtained at 40 and 60 after sowing, respectively. The occurrence of weeds was significantly reduced by barley living mulch. The early growth of soybean was reduced significantly because of the competition with barley living mulch, but the late growth of soybean after natural drying of barley living mulch was recovered considerably. The soybean grain yield with barley living mulch was similar to that of the normal soybean cultivation, showing no significant difference between no mulch and living mulch treatments. With respect to soil erosion on the slope soil, the amount of run-off water decreased by 20-50% and the amount of soil loss decreased by 70-90% by barley living mulch. The reduction in soil erosion by the use of plastic film + barley living mulch was similar to that of only barley living mulch. The barley living mulch in soybean production can be a promising cultivation technique as it reduces weed occurrence and soil erosion without grain yield reduction.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.352-352
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• 2017

Recently, the South Korean government encourages the cultivation of upland crops in the paddy field to maintain an adequate level of rice production and then to balance the demand and supply of rice. This is mainly because the rice consumption per capita per year has continued to decline from 135 kg in 1979 to 61.9 kg in 2016, although the rice production was relatively stable. As a result, the rice overproduction became a big social problem. As a part of that, various upland crops such as soybean, maize, minor cereals and forage crops are planted in the paddy field 10 years ago. The cultivation of these crops may settle the problem of short supply and mass import of the crops to some extent. However, a systematic remote observation of upland crops in the paddy field is very scarce. This study investigated the cultivation status of upland crops and any changes of crop harvesting in the paddy field by using an unmanned aerial vehicle (UAV). Also, we analyzed the kind of upland crops and cultivation area in the paddy field by utilizing time series observation images. A fixed wing UAV is used for the investigation. This is because it is easy to use the flight operation and to control flight management software, and it can automatically cope with various emergency states such as a strong wind and battery discharge. The material of UAV is expanded polypropylene, which has an advantage of less equipment damage and risk during takeoff and landing. We acquired observed images in Buljeong-myeon, Goesan-gun, Chungcheongbuk-do, South Korea by using fixed wing UAV in 2015 and 2016. The total investigated area reaches 6,045 ha, and among them the agricultural area was 1,377 ha. For the next step, we created an orthoimage from all images taken using Pix 4D mapper program. According to the results of image analyses in 2015, the paddy field covered total 577 ha (75.9%) with crop plant. The cultivation area of beans, ginseng, maize, tobacco and peach was 256 ha (36.6%), 63 ha (9.2%), 37 ha (5.4%), 31 ha (4.5%) and 27 ha (3.8), respectively. And in 2016, the total covered area was 586 ha (77.1%), and it was comprised of 253 ha (35.5%), 88 ha (12.3%), 29 ha (4.1%), 22 ha (3.1%) and 32 ha (4.5%) in the same order. In this study, we focused on identifying the paddy field which was converted to the cultivation of upland crops by using UAV. And, it has been indicated that the cultivation area of rice decreased from 141 ha in 2015 to 127 ha in 2016, although that of ginseng increased by 25 ha. As a result, it is expected that a lot of paddy field could be replaced by high-income crops such as ginseng and fruit tree (peach) instead of relative low-income rice. More specific and widespread research on the remote sensing in the paddy field needs to be done.