• Korean Journal of Organic Agriculture
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• v.22 no.4
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• pp.773-787
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• 2014

Bacterial pustule of soybean caused by Xanthomonas axonopodis pv. glycines is one of the most prevalent bacterial diseases in many areas where soybeans are grown. This study was carried out to evaluate the effect of cultivars, sowing date and cropping system on the suppression of soybean bacterial pustule in the field. One hundred soybean cultivars were screened for disease resistance against bacterial pustule in naturally infested field. Among them, fourteen cultivars including 'Pureun' were found to be high resistant. And thirty cultivars showed to be moderate resistant(less than 3% of diseased leaf area). When Soybean cultivar 'Taekwang' were sown in four different dates, May 25, June 5, June 15, and June 25, at 10 day-interval in Milyang, the diseased leaf area of bacterial pustule was 23.3%-25.7%, 14.7%-18.0%, 10.7%-12.8%, and 1.0%-2.7%, respectively. The lowest percentage of diseased leaf area was recorded in the plots sown on June 25, whereas the highest percentage of diseased leaf area was recorded in the plot sown on May 25. As sowing time was delayed, incidence of soybean bacterial pustule found to be comparatively reduced. From December in 2006 to June in 2007, we surveyed the pathogen population of soybean bacterial pustule in five cropping upland soils where soybean was cultivated. The survey result showed the bacterial pustule pathogens were detected from the all cropping soils. The pathogen populations of soybean bacterial pustule in soybean-barley and soybean-garlic cropping soil were significantly lower than that of the other cropping soils. In addition, the incidence of soybean bacterial pustule was decreased under the two cropping systems.

• Journal of Applied Biological Chemistry
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• v.44 no.4
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• pp.173-176
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• 2001

This report concerns the role of indole-3-acetic acid (IAA) in bacterial pustule disease of soybean. Pustule production in soybean leaves caused by Xanthomonas axonopodis pv. glycines was accompanied by a drastic increase in IAA content of host tissues. The phytopathogenic bacterium synthesized IAA in a tryptophan concentration-dependent manner when grown in a defined minimal medium. In complex media, however, the pathogen showed no response to tryptophan feeding, implying that the bacterial biosynthetic machinery of IAA is strictly regulated by nutrient availability of its growth environments. The results may suggest that IAA of bacterial origin and tryptophan of plant origin be involved in the process of pustule symptom development in soybean.

• Research in Plant Disease
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• v.16 no.3
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• pp.266-273
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• 2010

Bacterial pustule of soybean (Glycines max) caused by Xanthomonas axonopodis pv. glycines is one of the most prevalent bacterial diseases of soybean. This bacterium shows strong pathogenicity to the plants and distributes throughout Korea. However, no good control measures including bactericides and resistant cultivars are available to control the disease in Korea. Therefore, this study was conducted to develop chemical control method against soybean bacterial pustule. The present study was undertaken to find out the growth inhibitory effect bactericides (8 antibiotics, 2 copper compounds, quinoline, 18 agro-chemicals) on bacterial pustule pathogen. Antibiotics test showed that tetracycline and streptomycin sulfate significantly suppressed the growth of bacterial pustule pathogen. Also, application of oxolinic acid was found to be effective for pathogen inhibition. However, vancomycin, polymyxin B sulfate and copper compounds did not show the positive suppressive effect on growth of the pathogen. Among the eighteen agro-chemicals, streptomycin sulfate + oxytetracyclin (18.8 + 1.5%) WP, oxytetracycline (17%) WP and oxolinic acid (20%) WP were found to be effective for the inhibition of the pathogen in vitro. The selected 5 agro-chemicals were also applied on soybean in field and their control effects against the soybean bacterial pustule were tested. The foliar application of streptomycin sulfate + oytetracyclin WP and oxytetracycline WP on the naturally infected soybean (Taekwangkong) showed high control value (above 70%). Therefore, it is concluded that the bactericides used in this study showed strong inhibitory effect to soybean bacterial pustule and they can be recommend to farmers to control the disease.

• Research in Plant Disease
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• v.21 no.1
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• pp.40-43
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• 2015

Bacterial pustule of soybean is caused by Xanthomonas axonopodis pv. glycines, one of the most important diseases in soybean. The symptom of bacterial pustule is mainly distributed around leaf veins. However, the reason has not been known. In order to determine pathosystem of bacterial pustule in leaf, soybean leaves were collected and observed using scanning electron microscopy (SEM) and light microscopy. Many hairs were observed at abaxial sides of the leaf, few hairs were observed at tissue around the leaf veins. In addition, unidentified bacterial cells and dusts at the no hair part near veins were observed. In the inoculation assays, the cells of X. axonopodis pv. glycines were observed near leaf veins. The imprint of underside of soybean leaves inoculated with X.axonopodis pv. glycines on PDA showed that the growth of bacteria around veins was observed but no bacterial growth at the part with leaf hairs. Our data demonstrated that soybean leaf hairs play an important role as a physical barrier for structural resistance of soybean against bacterial pustule pathogen.

• Research in Plant Disease
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• v.17 no.3
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• pp.317-325
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• 2011

Bacterial pustule of soybean (Glycine max) caused by Xanthomonas axonopodis pv. glycines is one of the most prevalent bacterial diseases of soybean in Korea, where it causes considerable yield loss. This study was carried out to develop yield prediction model for bacterial pustule by analyzing correlation between the percentage of diseased leaf area and yield. The severe disease incidence of soybean bacterial pustule caused yield losses by 19.8% in 2006 and 16.8% in 2007, respectively. Severity of bacterial pustule greatly affected on 100 seed weight and yield, but did not on stem length, number of branches per plant, number of pods per plant, number of seeds per plant. On the other hand, correlation coefficients between diseased leaf area and yield were $-0.93^*$('06) and $-0.77^*$('07), respectively. The regression equation obtained by analyzing correlation between the percentage of diseased leaf area and yield loss in 2006 and in 2007 was y = -3.2914x + 348.19($R^2$ = 0.8603) and y = -2.9671x + 302.08($R^2$ = 0.9411), respectively. These results will be helpful in estimating losses on a field-scale and thereby predicting the production of soybean.

• The Plant Pathology Journal
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• v.38 no.4
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• pp.372-382
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• 2022

Soybean is an important source of protein and for a wide range of agricultural, food, and industrial applications. Soybean is being affected by Xanthomonas citri pv. glycines, a causal pathogen of bacterial pustule disease, result in a reduction in yield and quality. Diverse microbial communities of plants are involved in various plant stresses is known. Therefore, we designed to investigate the microbial community differentiation depending on the infection of X. citri pv. glycines. The microbial community's abundance, diversity, and similarity showed a difference between infected and non-infected soybean. Microbiota community analysis, excluding X. citri pv. glycines, revealed that Pseudomonas spp. would increase the population of the infected soybean. Results of DESeq analyses suggested that energy metabolism, secondary metabolite, and TCA cycle metabolism were actively diverse in the non-infected soybeans. Additionally, Streptomyces bacillaris S8, an endophyte microbiota member, was nominated as a key microbe in the healthy soybeans. Genome analysis of S. bacillaris S8 presented that salinomycin may be the critical antibacterial metabolite. Our findings on the composition of soybean microbiota communities and the key strain information will contribute to developing biological control strategies against X. citri pv. glycines.

• Journal of Crop Science and Biotechnology
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• v.11 no.4
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• pp.263-268
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• 2008

Bacterial leaf pustule (BLP) caused by Xanthomonas axonopodis pv. glycines is a serious disease to make pustule and chlorotic haloes in soybean [Glycine max (L). Merr.]. While inheritance mode and map positions of the BLP resistance gene, rxp are known, no sequence information of the gene was reported. In this study, we made five near isogenic lines (NILs) from separate backcrosses (BCs) of BLP-susceptible Hwangkeumkong $\times$ BLP-resistant SS2-2 (HS) and BLP-susceptible Taekwangkong$\times$ SS2-2 (TS) through foreground and background selection based on the four-stage selection strategy. First, 15 BC individuals were selected through foreground selection using the simple sequence repeat (SSR) markers Satt486 and Satt372 flanking the rxp gene. Among them, 11 BC plants showed the BLP-resistant response. The HS and TS lines chosen in foreground selection were again screened by background selection using 118 and 90 SSR markers across all chromosomes, respectively. Eventually, five individuals showing greater than 90% recurrent parent genome content were selected in both HS and TS lines. These NILs will be a unique biological material to characterize the rxp gene.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.652-661
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• 2021

Xanthomonas citri pv. glycines (Xcg) is a major pathogen of soybean (Glycine max) in South Korea, despite the availability of soybean varieties with some resistance. We conducted a nationwide survey of the incidence and severity of bacterial pustule caused by Xcg. The percentage of infected fields was 7% to 17% between 2015 and 2017. We characterized the diversity of a nationwide collection of 106 Xcg isolates based on avrBs3 banding patterns. The isolates fell into 11 groups, each represented by a type strain; only two of these were similar to isolates collected from 1999 to 2002. The diversity of Xcg strains increased and the dominant strains changed between 1999 and 2017, with three new type strains comprising 44% of the isolates examined in 2012 to 2017. Pathogenicity tests did not show evidence for a shift in the races or aggressiveness of Xcg strains. Korean soybean cultivars, including the widely-grown Daewon cultivar, were susceptible to the 11 new type strains. The cultivar CNS, which carries the rxp resistance gene, was susceptible to most type strains, including two representing 83% of the Korean Xcg strains. In contrast, Williams 82, which also carries rxp, showed resistance to at least five type strains. Collectively, these results suggest that Williams 82 has resistance loci in addition to rxp. The widespread distribution of Xcg, the high virulence of the current endemic strains, and the low resistance of most Korean soybean cultivars collectively favor widespread disease in Korea in years that are favorable to pustule development.

• The Plant Pathology Journal
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• v.23 no.3
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• pp.193-202
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• 2007

Typical susceptible symptoms of the bacterial pustule disease caused by Xanthomonas axonopodis pv. glycines are pustule and chlorotic haloes that usually occur in leaves of Glycine max plants. The soybean genotype 'PI96188' showed an atypical response to all tested races X. axonopodis pv. glycines, accompanied with necrosis without chlorotic haloes on the underside of the necrotic symptoms. X. axonopodis pv. glycines 8ra grew to levels from 10 to 100 fold lower on PI96188 than on susceptible cultivar Jinjoo1, but 10-fold higher than on the resistant cultivar CNS. The chlorophyll content in PI96188 leaves remained unchanged until 12 days after bacterial infection. Ultrastructural observation showed that the infected leaf cells of PI96188 had intact normal chloroplasts compared to those of the susceptible cultivar Jinjoo1. Chloroplast degradation or the absence of chloroplasts in cells of the infected tissues was observed in Jinjoo1. Senescence-related ACS7 gene was significantly induced in PI96188 compared to those in Jinjoo1 at 2 days after inoculation. While photosynthesis-related rbcS gene showed the dramatic change in Jinjoo1, this gene was constitutively expressed in PI96188. However, expression of the defense-related genes, such as peroxidase and isoflavone synthase in the infected PI96188 leaves was similar to that in Jinjoo1. Together, these results suggest that the novel necrotic symptom in PI96188 is a kind of resistant response different from a typical hypersensitive response in the resistant genotypes.

• The Plant Pathology Journal
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• v.15 no.1
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• pp.57-61
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• 1999

Xanthomonas campestris pv. glycines causes bacterial pustule disease on susceptible soybean leaves and produces a bacteriocin, named glycinecinA, against most xanthomonads including Xanthomonas campestris pv. vesicatoria. One of the 5 isolated DNA regions responsible for bacteriocin production, a 1.7 kb DNA region for the glycinecinA gene, was used as a probe to detect the presence of the homolog DNA in other bacterial strains. Among 55 bacterial strains tested, only X. campestris pv. glycines showed the positive signal with glycinecinA DNA. Two oligomers, heu2 and heu4, derived from a glycinecinA DNA were used to carry out the polymerase chain reaction (PCR) analysis with chromosomal DNA from 55 different bacterial strains including 24 different strains of X. campestris pv. glycines, 9 different pathovars of xanthomonads, and other 22 bacterial strains of different genus and species. By separation of the PCR products on agarose gel, a 0.86 kb DNA fragment was specifically detected when X. campestris pv. glycines was present in the amplification assay. The 0.86 kb fragment was not amplified when DNA from other bacteria was used for the assay. Southern analysis with glycinecinA DNA showed that the PCR signal was obtained with X. campestris pv. glycines isolates from various geographic regions and soybean cultivars. Therefore, the 1.7 kb DNA region for the glycinecinA gene can be used for the pathovar-specific probe for the DNA hybridization and the primers heu2 and heu4 can be used for the pathovar-specific primers for the PCR analysis to detect X. campestris pv. glycines.