• Asian-Australasian Journal of Animal Sciences
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• 제15권12호
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• pp.1790-1797
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• 2002

Lactic acid bacteria were isolated from piglets and chicken and characterized. Lactic acid bacteria showing resistance to low pH and bile, adhesion to intestinal epithelium cells, and the inhibition of Escherichia coli and Salmonella spp. were identified as Lactobacillus acidophilus. L. acidophilus PF01 survived for 2 h in MRS broth adjusted to pH 2. L. acidophilus CF07 was less resistant than L. acidophilus PF01 to pH 2, but survived at pH 2.5 for 2 h. Both of isolates were able to grow in MRS broth containing 0.3% (w/v) bile, with L. acidophilus CF07 being more tolerant to bile than L. acidophilus PF01. L. acidophilus PF01 and CF07 adhered specifically to the duodenal and jejunal epithelium cells of piglet, and the cecal and duodenal epithelium cells of chicken, respectively. Both of isolates did not adhere to the epithelium cells of the various animal intestines from which they were isolated. When L. acidophilus was cultured with E. coli and Salmonella spp. in MRS broth, MRS broth containing 2% skim milk powder or modified tryptic soy broth at $37^{\circ}C$, L. acidophilus PF01 and CF07 inhibited the growths of E. coli K88 and K99, and S. enteritidis and S. typhimurium, respectively. Both of isolates were found to possess the essential characteristics of probiotic lactic acid bacteria for piglet and chicken.

• 동의생리병리학회지
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• 제19권5호
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• pp.1233-1237
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• 2005

Catechin products in green tea extract was prepared to investigate antibacterial activity on the pathogenic bacteria. Survival of pathogenic bacteria (MASA - methicillin resistant Staphylocouus aureus, E.coli O157 and S. typhimurium Sal-13) in tryptic soy agar(TSA) containing Catechin products powder incubated at various concentration was evaluated. TSA containing $0{\sim}2%(w/v)$ of Catechin products was inoculated approximately $10^4\;CFU/ml$ of pathogenic bacteria and incubated at $37^{\circ}C$ for 24 hours. The plate counting technique and clear zoon test were used to test survival effect of the Catechin products. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was derived from the survival curves of pathogenic bacteria. S. typhimurium Sal-13 was the most sensitive strain to Catechin products. This result suggested that Catechin products can be used as an effective natural antibacterial agent.

• 한국식품영양과학회지
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• 제34권7호
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• pp.1064-1070
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• 2005

본 연구에서는 국내에서 소비되는 일부 상응 식품의 비타민 E 함량을 측정하고자 하였다. 각 식품군들의 비타민 E는 검화벙법 또는 직접용매 추출법을 이용하여 추출하였으며 이를 순상 HPLC를 통하여 분리, 정량하였다. 육류에선 장조림이 1.08 $\alpha-TE$가장 높았으며, 어패류는 마른 오징어가 4.69 $\alpha-TE$로 높게 분석되었다. 해조류는 조미김이 $5.70\;\alpha-TE$로 가장 높은 값을 나타내었다. 유제품은 분유에서 $3.17\;\alpha-TE$, 두류에선 서리태가 $1.52\;\alpha-TE$로 가장 높은 값으로 나타났고, 견과류에선 해바라기씨가 $17.99\;\alpha-TE$노 가장 높은 수치를 보였다 조리가공식품류는 라면이 $1.96\;\alpha-TE$로 가장 높았으며, 전통가공 식품류에서는 고추장이 $3.48\;\alpha-TE$로 가장 높은 분석치를 나타내었다. 본 논문에서 분석된 자료는 식품 내 비타민 E 함량 분석을 통한 표준화된 영양성분표의 기반확립에 도움이 되리라고 생각한다.

• 한국식품영양과학회지
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• 제24권4호
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• pp.502-509
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• 1995

In other to investigate the hypolipidemic effect of dry edible leaves on serum lipids, four kinds of powdered leaves(persimmom, mulberry, perilla, and soy bean) were added to experimental diets at the levels of 5%(w/w). The diets were fed to 30 male Sprague-Dawley rats, aged 4 weeks and weighing 60$\pm$5g, for 3 weeks. Food intake was the largest in Group 1(control), with 5% of cellulose powder, while food efficiency was the highest in Group 2(persimmon). Total cholesterol level in serum was lower in Group 5(soybean) and Group 4(perilla) than the other groups. In comparison with Group 1, HDL-cholesterol level was very high in Group 4 and atherogenic index was low in Groups 4 and 5. Free cholesterol level was the highest in Group 2, and was the lowest in Group 1. Both LDL and LDL-cholesterol levels were the lowest in Group 1, while they were very high in Groups 2 and 3(mulberry). Triglyceride level was the lowest in Group 4, and the highest in Group 1. Except Group 3, all groups had lower phospholipid level than Group 1. Compared with Group 1, calcium level in serum was low in Groups 3, 4 and 5, magenesium level was low in Groups 2, 3 and 5, phosphate level was high in Group 4, and iron level was exceptionally low in Groups 4 and 5. In conclusion, Group 4(perilla) and Group 5 (soybean) showed a favorable effect in atherogenic index and serum levels of various lipids.

• 한국수정란이식학회지
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• 제28권4호
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• pp.325-335
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• 2013

The study was set for one year to measure the effects of concentrate supplementation on reproductive performances and semen quality in indigenous rams. The study was conducted at the Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh during the period from May 2011 to April 2012. Forteen ram lambs (4~5 months) were randomly divided into two equal groups (n=7); supplemented vs control. The animals of control group were maintained on natural grazing. Along with natural grazing the supplemented group was on supplemented feeding. The concentrate supplementation (Wheat bran, Crushed maize, Soy bean meal, Fish meal, DCP powder, Vitamin mineral premix, Salt) was provided @ 300 g/head /day to the supplemented group. Body weight, scrotal circumference, BCS and libido index were measured weekly. Age, body weight and scrotal circumference at puberty were recorded. Semen was collected once in a weak using artificial vagina and chilled at $5^{\circ}C$ for 48h for evaluation. Concentrate supplementation did not influence (p>0.05) body condition score, age, weight, scrotal circumference at puberty and libido index. Final body weight (kg), growth rate (g/d), scrotal circumference (cm) and scrotal growth rate (mm/15d) were significantly (p<0.05) higher in supplemented group of rams compared to control. Volume, concentration, motility and membrane potentiality of spermatozoa were varied significantly (p<0.05) in supplemented and control groups. However, density, mass motility, viability and sperm with normal acrosome, midpiece and tail were not differed insignificantly (p>0.05) in different observation times. It was concluded that concentrate supplementation with free grazing improved weight and scrotal circumference gain and semen production with increased quality in indigenous ram.

• 한국식생활문화학회지
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• 제10권4호
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• pp.301-310
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• 1995

The purpose of this study is to survey various recips of the roasted skewered beef (Sanjuk) with twenty three classical cookbooks written before 1943 in Korea. The recipes of the roasted skewered beef are found thirty times in the records, which can be classified into six groups such as the palm-type skewered beef (Sulhamyukjuk), little finger-type skewered beef, mixed skewered beef, juice skewered beef, little finger-type skewered internal organs, and wide-cut skewered internal organs. The palm-type skewered beef and the mixed skewered beef most frequently appeared in the records. The 'Sulhamyukjuk' in the 17th century were inherited while changing its name to 'Sanjuk' in the late 18th century, which is the origin of 'Bulkoki'. There were two types of the roasted skewered beef, the palm-type and the little finger-type; and the palm type preceded the little finger-type. They were used with no change until the 19th century. Actually the roasted skewered beef existed even in the 16th century, but were put down in the early 17th century. In the cooking of 'Sulhamyukjuk' the process of dipping shortly into cold water in the midst of roast seems to absolutely disappeared. Some recipes of the roasted skewered beef were lost, but most have been inherited in the similar way with simplification including little use of internal organs. The main ingredients consisted of parts of cattle such as fresh meat, intestines, heart, liver, omasum, tripe, head, sweet bread, and lung with various vegetables and mushroom. And the main seasonings were mixtures of oil, soy sauce, sesame seed powder, scallion stalk, pepper, sesame seed oil, and salt. And sometimes wine, vinegar, ginger, garlic, and sugar were added.

• Journal of Ginseng Research
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• 제48권4호
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• pp.417-424
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• 2024

Background: This research main objective was to evaluate a proliposomes (PLs) formulation for the enhancement of oral bioavailability of ginsenosides, using ginsenoside Rg3 (Rg3) as a marker. Methods: A novel PLs formulation was prepared using a modified evaporation-on-matrix method. Soy phosphatidylcholine, Rg3-enriched extract, poloxamer 188 (Lutrol® F 68) and sorbitol were mixed and dissolved using a aqueous ethanolic solution, followed by the removal of ethanol and lyophilization. The characterization of Rg3-PLs formulations was performed by powder X-ray diffractometry (PXRD), transmission electron microscopy (TEM) and in vitro release. The enhancement of oral bioavailability was investigated and analyzed by noncompartmental parameters after oral administration of the formulations. Results: PXRD of Rg3-PLs indicated that Rg3 was transformed from crystalline into its amorphous form during the preparation process. The Rg3-encapsulated liposomes with vesicular-shaped morphology were generated after the reconstitution by gentle hand-shaking in water; they had a mean diameter of approximately 350 nm, a negative zeta potential (- 28.6 mV) and a high entrapment efficiency (97.3%). The results of the in vitro release study exhibited that significantly more amount of Rg3 was released from the PLs formulation in comparison with that from the suspension of Rg3-enriched extract (control group). The pharmacokinetic parameters after oral administration of PLs formulation in rats showed an approximately 11.8-fold increase in the bioavailability of Rg3, compared to that of the control group. Conclusion: The developed PLs formulation could be a favorable delivery system to improve the oral bioavailability of ginsenosides, including Rg3.

• Asian-Australasian Journal of Animal Sciences
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• 제21권4호
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• pp.574-581
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• 2008

Two experiments, involving a total of 100 crossbred pigs (Hampshire$\times$Landrace$\times$Duroc) aged 5 weeks, were used to evaluate the effect of Roselle (Hibicus sabdariffa Linn.) calyx as an antioxidant and acidifier on growth performance. Experiment 1: growth performance response of pigs fed basal corn-soy diet was compared with that of pigs consuming diets that contained 4, 8 and 12% Roselle in powder form, 4 g/kg acidifier (Fra$^{(R)}$Acid Dry) or 100 mg/kg antibiotic (Aurofac$^{(R)}$). All diets were isocaloric and isonitrogenous. Twenty-four castrated male and twelve female pigs were randomly allocated into 6 treatments which comprised 3 replicates (2 male and 1 female) of 2 pigs each. The results demonstrated no beneficial weight gain and feed intake response among treatments at 7, 9 and 11 weeks of age (p>0.05). However, feed-to-gain ratios (FCR) of Roselle-fed groups were significantly different from the basal-diet group at 7 weeks of age (p<0.05). Pigs fed with 8% Roselle had the lowest FCR. Therefore, 8% was set as an appropriate level of Roselle in feed. Experiment 2 was conducted to determine antioxidant and acidifier properties. Pigs were fed similar diets to Experiment 1 except that Roselle was fed to only one treatment at the level of 8%. Sixty-four piglets, 32 castrated male and female each were randomly allocated into 4 treatments which comprised 4 replicates (2 male and 2 female) of 4 pigs each. Body weight was measured. At 7 and 9 weeks of age, four pigs from each treatment were randomly selected. The pH in their gastrointestinal tract, pepsin activity in stomach mucosa, trypsin activity in the pancreas and protein and fat digestibility in the ileum were measured to investigate acidifier properties. For antioxidant properties, malondialdehyde (MDA) and glutathione concentration in plasma and liver were used as parameters. The results showed no significant difference in weight gain, pH or pepsin activity among all treatments at both ages (p>0.05). Nevertheless, trypsin activity and fat digestibility of the Roselle-fed group were higher than the basal-diet group at 7 weeks of age (p<0.05). Glutathione in plasma was significantly different between antibiotic-fed and basal-diet groups (p<0.05). Significant differences were found only in some parameters and between the basal-diet group and the other groups. Therefore, it cannot be precisely concluded that Roselle in powder form has clear acidifier and antioxidant properties in postweaning pigs. Further studies should be undertaken to clearly confirm both properties.

• 한국식품조리과학회지
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• 제28권3호
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• pp.327-335
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• 2012

탕평채의 전통 조리법 발굴을 위해 1700년대부터 1960년대의 고문헌 및 고조리서에 수록된 탕평채의 재료, 양념, 조리방법의 변화 등을 검토하였다. 탕평채는 1700년대 말의 문헌인 "경도잡지"와 "고사십이집"에 처음 소개된 후 "임원십육지", "동국세시기", "송남잡식", "진찬의궤", "진작의궤", "명물기략", "규곤요람", "시의전서", "조선요리제법", "조선무쌍신식요리제법", "주부의 동무, 조선요리제법", "조선요리학", "이조궁정요리통고", "우리나라 음식 만드는 법" 등에 탕평채가 수록되었다. 조선시대 궁중 연회음식을 수록한 "진찬의궤"와 "진작의궤"에는 청포채란 명칭으로 탕평채가 소개되어 있다. 탕평채의 재료 중 묵, 고기, 미나리, 숙주나물, 달걀, 김의 사용이 보편화된 것은 1900년대 중반 이후이며, 달래, 물쑥과 같은 봄나물을 재료로 사용한 기록도 있다. 1700년대 말에는 단지 초장(간장과 초로 만듦)으로만 무쳐 먹었던 탕평채는 이후 간장, 초 뿐 아니라 고추, 후춧가루, 깨소금, 참기름, 마늘, 파, 소금, 설탕 등을 사용해서 양념하여 보다 다양한 맛을 내고자 하였다. 또한 미나리, 고춧가루, 실고추, 지단채, 통잣, 잣가루, 깨소금, 김 등을 고명으로 사용했다. 탕평채의 조리방법은 1800년대 말의 문헌부터 비교적 자세히 기술되어 있다. 1800년대 말에는 모든 재료를 함께 양념했으나, 1900년대에는 다른 재료를 먼저 양념하고 마지막에 묵을 넣고 무치는 방법도 사용했다.

• 생명과학회지
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• 제12권1호
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• pp.8-15
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• 2002

동결된 오리 MDM을 $25^{\circ}C$에서 약 3시간 방치하여 반해동시킨 후 얇게 자르고, 저온의 알칼리액(0.2% NaHCO$_3$와 0.15% NaCl 혼합액)으로 4시간 침지.교반하면서 수세처리한 후 다시 염지액 중에서 4시간 동안 교반하며 염지하였다. 염지가 끝난 육을 수세한 후 수분 80%이하로 탈수하여 silent cutter에 넣고 육만을 10분간 1차 고기갈이하고, 다시 식염을 첨가하여 30분간 2차 고기갈이를 하였다. 나머지 부원료와 첨가물, 즉 0.3% 폴리믹스 GA, 0.3% 폴리믹스 CS, 0.3% 피로인산염, 0.3% 중합인산염, 4.2% 설탕, 8% 전분, 7% 대두분리단백, 0.2% MSG, 0.5% 양파분말, 0.1% 마늘분말, 1.5% 넛멕, 0.1%이하의 솔빈산칼륨, 0.0075% 식용색소 적색 40호 7% 난백, gluten 3% 및 돈지 10%를 넣고 15분간 3차 고기갈이한 후 즉시 PVDC 필름에 공기가 들어가지 않게 충진하여 밀봉한 다음 9$0^{\circ}C$의 증기를 이용하여 80분간 가열하고 실온까지 급냉시켜 오리 MDM 소시지를 제조하였다.