• 한국작물학회지
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• 제49권1호
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• pp.61-68
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• 2004

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains $5\textrm{mgL}^{-1}$ kinetin, $0.5\textrm{mgL}^{-1}$ NAA, 2 % sucrose (or maltose), 3% sorbitol, and $500\textrm{mgL}^{-1}$ proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• 한국식물병리학회지
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• 제3권2호
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• pp.131-136
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• 1987

벼 품종 Brazos와 M-201의 도열병에 대한 단위시간당 감염속도와 점접종원으로 부터의 단위 거리당 확산경사는 밭논과 물논의 두 재배조건에 따라 크게 달랐다. 물논재배는 도열병의 감염속도를 늦추고 확산경사를 완만하게 하였다. 점접종원으로부터 거리별로 4지점에서 측정된 도열병의 감염속도는 거리에 따른 통계적인 유의차는 없었지만 점접종원으로부터의 거리가 멀어짐에 따라 빨라지는 경향이었다. 품종별 감염속도는 Brazos보다 더 이병성인 M-201 품종에서 높았고 확산경사도 M-201 품종에서 가파른 경향이었다. 그러나 도열병이 진전함에 따라 생성된 이차전염원 때문에 도열병 확산경사는 두 품종에서 모두 완만해졌다. 조사된 확산형사의 두 경험적 모델 중에서 Kiyosawa와 Shiyomi 모델이 Gregory 모델에 비하여 통계적 적합성이 높았다. 밭상태에서 단위시간당 도열병 isopaths 이동거리는 Brazos와 M-201 품종에서 각각 0.2m/일와 0.4m/일로 측정하였다. 이상의 결과, 도열병에 대한 품종저항성의 차이는 감염속도뿐만 아니라 확산경사의 측정에 의하여 효과적으로 감지될 수 있다고 생각된다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제6권1호
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• pp.22-29
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• 2006

We demonstrate highly manufacturable Multi-channel Field Effect Transistor (McFET) on bulk Si wafer. McFET shows excellent transistor characteristics, such as $5{\sim}6 times higher drive current than planar MOSFET, ideal subthreshold swing, low drain induced barrier lowering (DIBL) without pocket implantation and negligible body bias dependency, maintaining the same source/drain resistance as that of a planar transistor due to the unique feature of McFET. And suitable threshold voltage ($V_T$) for SRAM operation and high static noise margin (SNM) are achieved by using TiN metal gate electrode.

• Advances in materials Research
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• 제9권3호
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• pp.219-231
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• 2020

The aim of this study is to investigate the durability of fly ash based geopolymer mortar with and without protective coatings in aggressive chemical environments. The source materials for geopolymer are Fly ash and Ground Granulated Blast furnace Slag (GGBS) and they are considered in the combination of 80% & 20% respectively. Two Molarities of NaOH solution were considered such as 8M and 10M. The ratio of binder to sand and Sodium silicate to Sodium hydroxide solution (Na₂SiO₃/NaOH) are taken as 1:2 and 2 respectively. The alkaline liquid to binder ratio is 0.4. Compressive strength tests were conducted at various ages of the mortar specimens. In order to evaluate the performance of coatings on geopolymer mortar under aggressive chemical environment, the mortar specimens were coated with two different types of coatings such as epoxy and Acrylic. They were then subjected to different chemical environments by immersing them in 10% standard solutions of each ammonium nitrate, sodium chloride and sulphuric acid. Drop in compressive strength as a result of chemical exposure was considered as a measure of chemical attack and the drop in compressive strength was measured after 30 and 60 days of chemical exposure. The compressive strength results following chemical exposure indicated that the specimens containing the acrylic coating proved to be more resistant to chemical attacks. The control specimen without coating showed a much greater degree of deterioration. Therefore, the application of acrylic coating was invariably much more effective in improving the compressive strength as well as the resistance of mortar against chemical attacks. The results also indicated that among all the aggressive attacks, the sulphate environment has the most adverse effect in terms of lowering the strength.

• 한국항행학회논문지
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• 제18권4호
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• pp.364-370
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• 2014

본 논문에서는 트렌치 게이트 MOSFET에 적용을 위한 고 신뢰성을 갖는 트렌치 형성기술과 고품격의 제조기술을 제안하였다. 이는 향후 전력용 MOSFET 에 널리 적용이 가능하다. 트렌치 구조는 DMOSFET에서 셀 피치크기를 줄여서 Ron 특성을 개선하거나 대다수 전력용 IC에서 전력용 소자를 다른 CMOS(Complementary Metal Oxide Semiconductor) 소자로부터 독립시킬 목적으로 채용된다. 마스크 레이어를 사용하여 자기정렬기술과 산화막 스페이서가 채용된 고밀도 트렌치 MOSFET를 제작하기 위한 새로운 공정방법을 구현하였다. 이 기술은 공정 스텝수를 감소시키고 트렌치 폭과 소오스, p-body 영역을 감소시킴으로써 결과적으로 셀 밀도와 전류 구동성능을 증가시키며 온 저항의 감소를 가져왔다.

• 대한의생명과학회지
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• 제19권4호
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• pp.330-337
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• 2013

There are several methods currently being used to diagnose tuberculosis in patients, such as smear, PCR, tuberculosis culture and X-ray. For a proper medical treatment, antimicrobial susceptibility test and rapid drug susceptibility testing have been operated. Tuberculosis bacilli usually need 3~8 weeks of culture period because of delay in RNA synthesis and require 15~22 hours for generation. After a germ raises in culture, we initiated antimicrobial susceptibility test for a proper treatment. It has some difficulties to give a proper prescription for a tuberculosis patient because antimicrobial susceptibility test requires 4 weeks. To supplement this, we are practicing drug susceptibility testing which allow us to know the sensibility of RMP and INH after 2 or 3 days. But this is only possible when more than 2 positive germ. Therefore, we should practice rapid drug susceptibility testing with culture test. But if media is contaminated by other germs except Mycobacterium tuberculosis, it's hard to interpret result about culture test and to practice antimicrobial susceptibility test and rapid drug susceptibility testing. Because we have to practice again smear, culture test after extracting specimen from the patient, time is consumed and proper patient treatment is postponed. To address these problems and quick patient treatment, rapid drug susceptibility testing is practiced by using GenoType$^{(R)}$ MTDRplus method. As a result of this method we detected sensibility 10 and 7 cases and resistance 0 and 3 cases using RIM and INH respectively with other 1 case toward medicals out of the total 11 test. In conclusion rapid drug susceptibility testing can be used from the contaminated specimen after elimination of contaminated source from culture and proved that it can be practiced for rapid examination of a tuberculosis patient.

• 한국재료학회지
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• 제21권8호
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• pp.468-475
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• 2011

[ $LaMeO_3$ ](Me = Cr, Co) powders were prepared using the polymeric precursor method. The effects of the chelating agent and the polymeric additive on the synthesis of the $LaMeO_3$ perovskite were studied. The samples were synthesized using ethylene glycol (EG) as the solvent, acetyl acetone (AcAc) as the chelating agent, and polyvinylpyrrolidone (PVP) as the polymer additive. The thermal decomposition behavior of the precursor powder was characterized using a thermal analysis (TG-DTA). The crystallization and particle sizes of the $LaMeO_3$ powders were investigated via powder X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and particle size analyzer, respectively. The as-prepared precursor primarily has $LaMeO_3$ at the optimum condition, i.e. for a molar ratio of both metal-source (a : a) : EG (80a : 80a) : AcAc (8a) inclusive of 1 wt% PVP. When the as-prepared precursor was calcined at $700^{\circ}C$, only a single phase was observed to correspond with the orthorhombic structure of $LaCrO_3$ and the rhombohedral structure of $LaCoO_3$. A solid-electrolyte impedance-metric sensor device composed of $Li_{1.5}Al_{0.5}Ti_{1.5}(PO_4)_3$ as a transducer and $LaMeO_3$ as a receptor has been systematically investigated for the detection of NOx in the range of 20 to 250 ppm at $400^{\circ}C$. The sensor responses were able to divide the component between resistance and capacitance. The impedance-metric sensor for the NO showed higher sensitivity compared with $NO_2$. The responses of the impedance-metric sensor device showed dependence on each value of the NOx concentration.

• 한국표면공학회지
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• 제38권5호
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• pp.202-206
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• 2005

Nitriding treatments were conducted on tool steel (SKD 61) at a temperature of $500^{\circ}C$ for 5 hr using high vacuum direct current (DC) plasma, with ammonia and argon as source gases. The structural and compositional changes produced in the nitrided layers by applying different ratios of Ar to $NH_{3}\;(n_{Ar}/n_{NH3}) were investigated using glancing x-ray diffraction (GXRD), optical microscopy, atomic force microscopy (AFM), micro-Vickers hardness testing, and pin-on-disc type tribometer. Nitriding case depths of around of $50{\mu}m$ were produced, varying slightly with different ratios of $n_{Ar}/n_{NH3}. It was found that the specimen surface hardness was 1150 Hv with $n_{Ar}/n_{NH3}=1, increasing to a maximum value of 1500 Hv with $n_{Ar}/n_{NH3}=5. With a further increase in ratio to $n_{Ar}/n_{NH3}=10, the surface hardness of the specimen reduced slightly to a value of 1370 Hv. These phenomena were caused by changes of the crystallographic structure of the nitride layers, i.e the $\gamma'-Fe_{4}N$ phase only was observed in the sample treated with $n_{Ar}/n_{NH3}$=1, and the intensity of the $\gamma'-Fe_{4}N$ phase were reduced but new phase of $\varepsilon'-Fe_{3}N$, which was known as a high hardness, with increasing $n_{Ar}/n_{NH3}. Also, the relative weight loss of counterface of the pin-on-disc with unnitrided steel was 0.2. And that of nitrided steel at a gas mixture ($n_{Ar}/n_{NH3}) of 1, 5, 7, and 10 was 0.4, 0.7, 0.6, and 0.5 mg, respectively. This means that the wear resistance of the nitrided samples could be increased by a factor of 2 at least than that of unnitrided steel.

• 한국구조물진단유지관리공학회 논문집
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• 제20권3호
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• pp.33-41
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• 2016

콘크리트는 내화재료로서 우수한 성능을 발휘하지만 화재가 지속됨에 따른 재료특성 변화 또는 성능저하의 위험을 갖는다. 이 연구는 실물모형 철근 콘크리트 (RC) 보를 활용하여 비재하 화재 실험을 수행하여 화재노출 전후의 콘크리트 및 보강철근의 재료특성을 실험적으로 분석한 연구이다. 화재실험에 사용된 보는 길이 4 m의 RC 보로서 KS F 2257 화재실험 규격에 따라 시험 체를 제작 및 화재실험을 수행하였다. 화원의 가력은 ISO 834의 표준화재 곡선을 사용하였으며 보 가열부에서의 온도를 계측하고자 하면 및 측면에 열전대를 설치하였다. 실험결과, 화재에 노출된 화재 코어 공시체의 경우 약 11 MPa로 약 66%의 강도저하가 발생하였다. 화재에 직접 노출된 철근의 경우 노출되지 않은 철근에 비해 약 17%에 해당하는 75 MPa의 항복강도 저하를 나타낸 것으로 분석되었다. 철근의 경우 콘크리트라는 내화피복에 의하여 보호되어 약 4시간의 화재 실험에서도 온도는 한계온도의 최댓값인 $649^{\circ}C$를 크게 상회하지 않는 것으로 나타났다.

• Journal of Electrochemical Science and Technology
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• 제7권2호
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• pp.146-152
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• 2016

A combined system with PEMFC and reformer is introduced and optimized for the real use of this kind of system in the future. The hydrogen source to operate the PEMFC system is methanol, which needs two parts of methanol reforming reaction and preferential oxidation (PROX) for the hydrogen fuel process in the combined operation PEMFC system. With the optimized methanol steam reforming condition, we tested PROX reactions in various operation temperature from 170 to 270 ℃ to investigate CO concentration data in the reformed gases. Using these different CO concentration, PEMFC performances are achieved at the combined system. Pt/C and Ru promoted Pt/C were catalysts were used for the anode to compare the stability in CO contained gases. The alloy catalyst of PtRu/C shows higher performance and better resistance to CO than the Pt/C at even high CO amount of 200 ppm, indicating a promotion not only to the activity but also to the CO tolerance. Furthermore, in a system point of view, there is a fluctuation in the PEMFC operation due to the unstable fuel supply. Therefore, we also modified the methanol reforming by a scaled up reactor and pressurization to produce steady operation of PEMFC. The optimized system with the methanol reformer and PEMFC shows a stable performance for a long time, which is providing a valuable data for the PEMFC commercialization.