• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.516.2-516
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• 1986

DNA subfragments, sopA, sopB, and sopC supporting stable maintenance of an oriC plasmid, were derived from mini-F plasmid DNA (EcoRI restriction fragment, f5) after digestion with restriction endonucleases, and cloned in vector plasmid pBR322. The recombinant plasmid obtained were introduced into E. coli KY7231 and E. coli CSR603, and proteins specified by the mini-F fragments were analysed by SDS-polyacrylamide gel electrophoresis. Two proteins encoded by the F fragments were detected, having molecular weights of 41,000 and 37.000. The sopA protein (41K) encoded by a plasmid pXX288 was observed in the cytoplasm, whereas the sopB protein (37K) encoded by a plasmid pXX157 was in the membrane fraction. There was no novel protein band detected in the cell with a plasmid pXX300, which contained sopC fragment. Gene products of a plasmid pXX167, which is comprised of sopA, sopB, and sopC, were not detectable. Fluorography after one and two dimensional gel electrophoresis of the lysates showed that these two proteins were overproduced in the cells which were allowed to incorporate radioactive amino acid after plasmid amplification by chloramphenicol treatment. The isoelectric points of the sopA and sepB proteins were 6.6 and 7.0, respectively.

• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.256-260
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• 1993

DNA subfragments, sopA, sopB and sopC which help to maintain the stability of an ori C plasmid, were derived from a mini-F plasmid DNA (EcoRI restriction fragment f5) after digestion with restriction endonuclease, and cloned in the vector plasmid pBR322. The recombinant plasmids obtained were introduced into E. coli KY7231 and E. coli CSR603 strains, and proteins specified by the mini-F fragments were analysed by SDS-PAGE. Two proteins encoded by the F fragments were detected, and their molecular weights were 41,000 and 37,000 daltons. Fluorography after one and two dimensional gel electrophoresis of the lysates showed that these two proteins had been overproduced in the cells which were allowed to incorporate radioactive amino acid after plasmid amplification by chloramphenicol treatment. The isoelectric points of sopA and sopB proteins were 6.6 and 7.0, respectively.

• Korean Journal of Microbiology
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• v.36 no.4
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• pp.254-258
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• 2000

Invasion process of bacterial cell into intestinal epithelium is important in Salmonella infection. The invasion is induced by the proteins secreted by type III secretion appratus of Salmonella. It has been known that the proteins do not have N-terminal signal peptide existing in general secreted proteins. Recent studies on Yersinia reported that secretion signal of type III appratus may lie on 5'end secondary structure of mRNA of secreted protein. In this study, we constructed translational fusion of ompR and sopE, encoding type III secretion protein of Salmonella, and observed secretion of the fusion protein for investigating the secretion signal of Salmonella type III appratus. The sopE DNA fragments of the translational fusion contain the region of promoter and from start code to tenth or to fifth code. These translational fusions indicate that type III secretion signal of Salmonella is located on 5'end of mRNA encoding secreted protein. We constructed prototype of secretion vector using this signal to produce useful foreign protein.

• Composites Research
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• v.14 no.5
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• pp.46-53
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• 2001

In this research, damage behavior of singly oriented ply (SOP) fiber metal laminate (FML) subjected to concentrated load was studied. The static indentation tests were conducted to study fiber orientation effect on damage behavior of FML. During the static indentation tests, acoustic emission technique (AE) was adopted to study damage characteristics of FML. AE signals were obtained by using AE sensor with 150kHz resonance frequency and the signals were compared with indentation curves of FML. The damage process of SOP FML was divided by three parts, i.e., crack initiation, crack propagation, and penetration. The AE characteristics during crack initiation show that the micro crack is initiated at lower ply of the plate, then propagate along the thickness of the plate with creating tiber debonding. The crack grow along the fiber direction with occurring 60∼80dB AE signal. During the penetration, the fiber breakage was observed. As fiber orientation increases, talc fiber breakage occurs more frequently. The AE signal behaviors support these results. Cumulative AE counts could well predict crack initiation and crack propagation and AE amplitude were useful for the prediction of damage failure mode.

• The Journal of the Korean Society for Microbiology
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• v.35 no.5_6
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• pp.337-339
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• 2000

MRSA, erythromycin-resistant S. pyogenes, penicillin non-susceptible pneumococci, PPNG, ESBL-producing E. coli and K. pneumoniae, class C ${\beta}$-lactamase-producing E. coli, fluoroquinolone-resistant E. coli, aminoglycoside-resistant A. baumannii and P. aeruginosa are all prevalent in Korea, which suggest the presence of high levels of antimicrobial selective pressure and nosocomial spread of resistant bacteria. Rapid increase of VRE and emergence of fluoroquinolone-resistant gonococci and VIM-2 metallo-${\beta}$-lactamase-producing P. aeruginosa are recently observed new threats in Korea.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.3
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• pp.372-380
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• 2003

Impact tests were conducted to study the effect of angle ply and metal laminate on impact damage characteristics of Fiber Metal laminates (FML). Impact tests were conducted using drop weight impact machine and damage behavior were analyzed by comparing with load-displacement curve and surface observation and microscopic observation of cross sections. The effect of angle ply on impact characteristics of FML are influenced by property of metal laminate. i.e., when the metal laminate is not enough to strong to prevent fiber debonding, Angle ply FML is superior to singly oriented ply (SOP) FML because angle ply enhance the stiffness by fiber supports and prevent (rack propagation. However, when the metal laminate is enough to strong to prevent fiber debonding, SOP FML is superior to Angle ply FML because the fiber of lower ply in Angle ply FML are more stressed than that of SOP FML.

• Journal of Animal Environmental Science
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• v.19 no.2
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• pp.81-88
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• 2013

We planned to study how the chemical disposal designed by SOP can affect on physicochemical compositions of the livestock excrement. According to Livestock Manure Management Scheme, we experimented in two steps; the first step, NaOH treatment-Citric acid neutralization, and then the second procedure, Thermophilic Aerobic Oxidation (TAO) system. Physicochemical compositions of the 3-days-old samples after NaOH treatment were pH 10.31, EC 24.54 mS/cm, SCOD 3,022 mg/L, T-N 4,315 mg/L, $NH{_4}^+-N$ 1,960 mg/L, and not detected E. coli.. And those of one-day-old samples after citric acid neutralization were pH 7.36, EC 32.89 mS/cm, $SCOD_{Mn}$ 12,733 mg/L, T-N 4,787 mg/L, $NH{_4}^+-N$ 2,450 mg/L, and E. coli. not detected. In contrast, the physicochemical compositions of the treatment plots after the second treatment with TAO system (72hr) were pH 9.42 EC 24.21 mS/cm, $SCOD_{Mn}$ 3,660 mg/L, T-N 3,616 mg/L, $NH{_4}^+-N$ 1,190 mg/L, and no detection of E. coli.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.6
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• pp.2649-2669
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• 2020

In this paper, we analyze the secrecy performance of single-input multiple-output (SIMO) relay systems over κ-μ shadowed fading channels. Based on considering relay model employing decode-and-forward (DF) protocol, two security evaluation metrics, namely, secure outage probability (SOP) and probability of strictly positive secrecy capacity (SPSC) are studied, for which closed-form analytical expressions are derived. In addition, Monte Carlo results prove the validity of the theoretical derivation. The simulation results confirm that the factors that enhance the security include large ratio of (μ_D, μ_E), (m_D, m_E), (L_D, L_E) and small ratio of (k_D, k_E) under the high signal-to-noise ratio regime.

• The Journal of Fisheries Business Administration
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• v.37 no.1 s.70
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• pp.111-120
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• 2006

식품관련 시장조사 기관인 Food marketing Instittue에 따르면, 미국전체의 식료품체인 가운데 25%가 최소한 온라인 쇼핑을 운영하고 있다. Safeway, Albertsons 등 대형 수퍼마켓 체인들이 연이어 온라인 시장에 참가했고, Melon Seed, FreshDirect.com 등 신규업체도 참가했다. 2002년 5월 Safeway.cm을 설립한 Safeway는 동년 7월에 8번째 점포를 시애틀에 오픈하는 등, 온라인 비즈니스를 확대했다. 최근 할인매장, 드러그 스토어 둥에 일부 시장을 잠식당하고 있는 이들 전통적인 수퍼마켓 체인들은 고객의 이탈을 막기 위해 비록 소수이기는 하지만 고객의 온라인 수요에 대응할 필요성이 있다고 생각하기 때문이다. 본고의 목적은 미국의 식료품 온라인 쇼핑에서 발생하고 있는 여러 문제점을 지적하는 것이다. Webvan이 Dot Com의 실패를 예증하는데 사용되는 주요한 전자상거래 비즈니스이다. 오늘날 온라인 쇼핑 비즈니스의 도전을 보여주고 이익을 낳기 위해 취해야 할 조치를 논의하기 위해 온라인 쇼핑 밴더가 참조되고 있다. 또한 본고는 미국의 Vons.com, Peapod.com 그리고 Safeway.com과 같은 온라인 식료품 쇼핑 비즈니스에서 나타나고 있는 추세, 신규 영업자 및 잔존 영업자들의 특성과 온라인 식료품 쇼핑업자들의 성장에 필요한 요인들에 관해서도 논하였다.

• Journal of the Korea Society of Computer and Information
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• v.12 no.2 s.46
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• pp.37-45
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• 2007

In this paper, we proposed a new customized e-Ring(Enterprise Ring Back Tone) system which could be represented each user and organization feature. The e-Ring system provided the ring back tone and additional services to reduce the waiting time when the client called an organization. This system was for an appropriate organization public relations instead of the meaningless ring back tone which was used usual call, also was maximized the service availability and work efficiency which was obtained from the automatic response and service suppling to users who had needs and questions.