• Title/Summary/Keyword: sonic

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Fractionation of the Cells of Staphylococcus aureus (Staphylococcus aureus의 균체분획(菌體分劃))

  • Choi, Kyoung-Ho;Hyun, Eun-Min;Park, Kum-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.10 no.1
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    • pp.85-91
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    • 1981
  • The growing cells of S. aureus were fractionated along the Schmidt-Thannhauser-Schneider's technique into several fractions such as TCA(trichloroacetic acid)-soluble, lipid, nucleic acid, protein and residue fraction. They were also fractionated according to their cellular structure into Sonic-supernatant, SDS(sodium lauryl sulfate)-soluble, Formamide-soluble and Residue fraction. Fractionation was carried out by orderly treatment of the Sonic pellet with 1.0% SDS and hot$(150^{\circ}C)$ formamide, and the pellet was prepared by centrifugation of the cells sonic osillated for 20 minutes at 150 watt. Sonic-supernatant fraction contained a 91.3% of total DNA while other fractions contained less than 9.5%. SDS-soluble fraction showed a high activity of malate dehydrogenase(13.67 unit/mg protein) and which was higher 22.3 times than the activity found from unsoluble fraction. Formamide-soluble fraction prepared from SDS-undoluble pellet by using the hot formamide exhibited a clear action of reducing sugars against the Anthronesulfate, while it exhibited no clear action against the ninhydrin. However, contrastly, the residue remainnning after extraction with formamide exhibited a clear action against ninhydrin and glucosamine was detected form the hydrolysate of residue by paper chromatography. From these results it is considered that the Sonic-supernatant fraction is mainly consisted of plasmic component of the cells. Other fractions, SDS-soluble, Formamide-soluble and Residue, should be consisted of plasma membrane, lipoplysaccharide and peptidoglycan of the cell, respectively.

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Analysis of inverter switched snubber using N-channel MOS-FET

  • Suzuki, Taiju;Ikeda, Hiroaki;Mizutani, Yoko;Ishikawa, Jinichi;Yoshida, Hirofumi
    • 제어로봇시스템학회:학술대회논문집
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    • 1996.10a
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    • pp.207-210
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    • 1996
  • This paper describes the analysis of the operation of the switched snubber in order to depress the surge voltage in the MOS-FET inverter. In this paper, the N-channel MOS-FET which operates faster than the P-channel MOS-FET was used for the inverter circuit. So, the inverter and switched snubber can operate at high-frequency in the order of MHz. The cause of generating the surge voltage in the high frequency inverter has been cleared, and then how to depress the surge voltage using the switched snubber consisting of an N-channel MOS-FET has been given. Furthermore, described is the power loss within the switched snubber which is made of an N-channel MOS-FET. The inverter having the N-channel MOS-FET used as a switched snubber can drive such a low impedance load such as mega-sonic transducer for a mega-sonic studied cleaner sufficiently.

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Application of Preconditioning to Navier-Stokes Equations (예조건화 방법론의 Navier-Stokes 방정식에의 적용)

  • 이상현
    • Journal of the Korean Society of Propulsion Engineers
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    • v.8 no.1
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    • pp.16-26
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    • 2004
  • The objective of this study is to apply preconditioning to Wavier-Stokes equations with a turbulence model. The concept of a pseudo sonic speed was adopted. Roe's FDS was used for spatial discretization, LU-SGS scheme was used for time integration. In order to test the algorithms, the low speed flows around NACA airfoils and the flows through supersonic nozzle were calculated. The algorithm developed in the present study shows good performance in the calculations of low speed viscous flows and supersonics flows.

Effect of Grain Size on the Ballistic Performance of Alumina Ceramics (알루미나의 방탄특성에 대한 입경의 영향)

  • 백용기;강을손;정동익;최원봉
    • Journal of the Korean Ceramic Society
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    • v.29 no.4
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    • pp.312-318
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    • 1992
  • Two kinds of alumina specimens with different grain size (1 and 51 $\mu\textrm{m}$) but same density were prepared by hot-pressing. Fracture strength and fracture toughness of these specimens at low strain rate, sonic velocity, and elastic property were evaluated. Ballistic performance against Cal. 50 AP projectile was characterized by thick-backing method by using A16061-T6 reference block. Mechanical properties measured at low strain rate showed that the specimen with samll grain (SG) were better than specimen with large grain (LG). Fracture strength and fracture toughness of LG specimen were 131 MPa and 3.01 MPa{{{{ SQRT { m} }}, but those of SG specimen were 349 and 4.23, respectively. Sonic velocity and elastic properties of these specimen were similar, but bulk velocity and bulk modulus were different at amount of 4 and 9%. The tendency of ballistic performance was not consistent with the mechnaical properties at low strain rate. The ballistic performance based on quantitative efficiency revealed that the LG specimen (5.13) was ballistically better than the SG specimen (4.00) in spite of their lower mechanical properties.

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A Study on the Application of NDT(Non-Destructive Testing) Techniques to the Unknown Bridge Foundations (미지의 교량기초에 대한 비파괴 시험기법(NDT)의 적용성에 관한 연구)

  • 채종훈;이원제;유재명;이우진
    • Proceedings of the Korean Geotechical Society Conference
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    • 2000.03b
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    • pp.317-324
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    • 2000
  • In this study, ten drilled shafts were constructed for evaluating the application of NDT(Non-Destructive Testing) techniques. The drilled shafts, 0.4 m in diameter and 7.0 m in length, were constructed at Namyangju site in Namyangju City. One of the shafts was constructed with no defect, and the other shafts were constructed with the defects of soft bottom, necking, bulging, cave-in and/or weak concrete. Then, these techniques were applied to the bridge foundations for studying unknown bridge foundation characteristics.

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