• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.9
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• pp.1432-1438
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• 2004

This study investigated the inhibitory effect of methanol extracts and several solvent fractions from doen-jang on mutagenicity using in vitro SOS chromotest and in vivo Drosophila mutagenic system. In order to determine an antimutagenic effect of doenjang methanol extracts, other soybean fermented foods and original materials were compared. The treatment of doenjang methanol extracts (100 ${\mu}$/assay) to SOS chromotest system inhibited N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced mutagenicity by 87~97% and showed higher antimutagenic effect than other fermented foods. Among solvent fractions from doenjang methanol extracts, the ethylacetate and dichloromethane fractions showed the stronger antimutagenic effect (91% and 95%, respectively) in SOS chromotest. In Drosophila mutagenic system, the treatment of ethylacetate fraction (5%/bottle) significantly inhibited aflatoxin $B_1$ induced mutagenicity by 97%. These results demonstrated that doenjang had an inhibitory effect to mutagenic agents in both in vitro and in vivo mutagenic systems, suggesting that its antimutagenic effect may be due to active compounds in the ethylacetate fraction from doenjang methanol extracts.

• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.1-5
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• 2014

This study was performed to determine the whitening effect of organic solvent extracts from the centipede, Scolopendra subspinipes mutilans. We prepared different concentrations (50%, 70% and 100%) of ethanol, methanol, 100% ethyl acetate and water extracts. We tested melanin inhibitory effect and tyrosinase activity using B16/F10 melanoma cell. As a result, treatment of organic solvent extracts is decreased the biosynthesis of melanin and tyrosinase activity to 36 ~ 86%. Especially the 70% ethanol extracts was the most effective in B16/F10 melanoma cells. In the study on melanogenic protein expression, 70% ethanol extracts of Scolopendra subspinipes mutilans blocked glycosylation of tyrosinase. Therefore this result suggests that 70% ethanol extracts could be developed as skin whitening agents.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.11 no.2
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• pp.85-90
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• 1978

In present study, the effect of various factors including the solvent concentration, extraction time, extraction temperature and the ratio of sample vs extraction solvent(w/v) upon the extractability of the NaOH soluble proteins of marine algae were investigated. Seven species of sun-dried algae, the major ones in consumption as food, namely Porphyra suborbiculata, Undaria pinnatifida(natural and cultivated), Sargassum fulvellum, Sargassum kjellmanianum, Ulva pertusa, Enteromorpha linza and Codium coarctatum were used for the extraction of the NaOH soluble protein. The frozen and masceratd samples were prepared by the same mettled described in previous paper(Lee, 1977). In case of the TCA insoluble protein, all samples reached maxima at 0.025M NaOH solution while the 0.05M for extractable total nitrogen. Variation of the ratio of sample vs solvent gave slight effect upon the extractability, 100 ml solvent added to 1 g dried sample was effective. The effect of extraction time on the extractability differed from species. The extractabilty of Enteromorpha linza, Ulva pertusa and Codium coarctatum reached maxima within 1 hour extraction and 2 hours for the cultivated Undaria pinnatifida while 3 flours for the natural Undaria pinnatifida, Sargassum fulvellum, Sargassum kjellmanianum and Porphyra suborbiculata. The most effective extraction temperature was $60^{\circ}C$ for all samples.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.1
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• pp.54-59
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• 1997

After extracting Caridina denticulata denticulata, the raw material of salt-fermented Toha, with the use of water, ether, acetone and methanol in order, we had the antimicrobial activity test for nine strains of bacteria which are harmful to the human body and food. It is as follows; solvent extract yield of the powder of vacuum freeze dried Caridina denticulata denticulata was 52% of water fraction, 7.4% of ether fraction, 1.0% of acetone fraction and 0.8% of methanol fraction. The result of solvent extract antimicrobial search of Caridina denticulata denticulata said that with the sample of 20$\mu\textrm{g}$/disk, ether fraction resulted in clear one of 8~12mm, acetone fraction 8~12mm, methanol fraction 7~9mm and water fraction didn't have antimicrobial effect. The minimal inhibitory concentration of solvent extract of Caridina denticulata denticulata per area was that ether fraction was 250~500$\mu\textrm{g}$/$m\ell$, acetone fraction 125~500$\mu\textrm{g}$/$m\ell$ and methanol fraction 250~500$\mu\textrm{g}$/$m\ell$. The minimal bactericidal concentration of solvent extract per area of Caridina denticulata denticulata showed that ether fraction was 2, 500~10, 000$\mu\textrm{g}$/$m\ell$, acetone fraction 1, 250~10, 000$\mu\textrm{g}$/$m\ell$ and methanol fraction 5, 000~10, 000$\mu\textrm{g}$/$m\ell$.

• Food Science and Preservation
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• v.10 no.4
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• pp.540-546
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• 2003

Biological activities of solvent fractions obtained from Cnsicum annuum leaves, being used in material of functional food, were examined by the methods of DPPH scavenging activity, reducing power, nitrite scavenging activity, antimicrobial activity and inhibitory effect on tyrosinase activity. The highest yield was obtained from water fraction, where as the lowest yield was obtained from ethyl acetate traction, 16.9% and 0.6%, respectively. Hydrogen donating activity of Capsicum annuum leaves in increased with increasing amount of extract. Reducing power of the ethyl acetate fraction is increased as the amount of extract is increased. Even in the presence of 900 $\mu\textrm{g}$ of ethyl acetate fraction, reducing power was significantly higher than it was fer the control in which there was no extract. Among the various solvent fractions, ethyl acetate fraction showed the strongest scavenging effect on hydrogen peroxide. Nitrite scavenging effects of all concentrations diminished at higher pH, while in the case of pH 1.2, it showed a nitrite scavenging effect of more than 90% at concentration above of ethyl acetate fraction 500 $\mu\textrm{g}$. Among the various solvent fractions from methanol extract of Capsicum annuum leaves, ethyl acetate and butanol fraction showed the strongest antimicrobial activity. Antimicrobial activity of ethyl acetate fraction was 20 mm against Bacillus cereus, 18 mm against Staphylococcus aureus and 17 mm against Streptococcus mutans. Ethyl acetate fraction showed the strongest of inhibitory activity of tyrosinase.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.663-669
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• 2008

The solvent-tolerant bacterium Enterobacter sp. VKGH12 is capable of utilizing n-butanol and contains an $NAD^+$-dependent n-butanol dehydrogenase (BDH). The BDH from n-butanol-grown Enterobacter sp. was purified from a cell-free extract (soluble fraction) to near homogeneity using a 3-step procedure. The BDH was purified 15.37-fold with a recovery of only 10.51, and the molecular mass estimated to be 38 kDa. The apparent Michaelis-Menten constant ($K_m$) for the BDH was found to be 4 mM with respect to n-butanol. The BDH also had a broad range of substrate specificity, including primary alcohols, secondary alcohols, and aromatic alcohols, and exhibited an optimal activity at pH 9.0 and $40^{\circ}C$. Among the metal ions studied, $Mg^{2+}$ and $Mn^{2+}$ had no effect, whereas $Cu^{2+},\;Zn^{2+}$, and $Fe^{2+}$ at 1 mM completely inhibited the BDH activity. The BDH activity was not inhibited by PMSF, suggesting that serine is not involved in the catalytic site. The known metal ion chelator EDTA had no effect on the BDH activity. Thus, in addition to its physiological significance, some features of the enzyme, such as its activity at an alkaline pH and broad range of substrate specificity, including primary and secondary alcohols, are attractive for application to the enzymatic conversion of alcohols.

• Korean Chemical Engineering Research
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• v.49 no.3
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• pp.352-356
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• 2011

In this paper, we prepared new type of hydrophilic block copolymers that exhibit the long-ranged lateral ordering in thin film. It was previously demonstrated that poly(ethyleneoxide-b-styrene) and poly(ethyleneoxide-b-metharylate-b-styrene) block copolymer thin films have a high degree of lateral ordering after solvent annealing process. In these cases, the relative humidity plays an important role in long-ranged lateral ordering. However, the origin of the humidity effect on the orders of these hydrophilic block copolymers is not fully understood. To investigate the effect of the humidity further, we prepared other hydrophilic poly(N,N-dimethylacrylamide-b-styrene)(PDMA-b-PS) block copolymers via RAFT polymerization. As with PEO-containing block copolymers, PDMA-b-PS block copolymers exhibit the long-ranged lateral ordering after solvent annealing process.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.1
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• pp.1-7
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• 2005

The scavenging effects of four extracts on the DPPH radical were increased with increasing amount of extract. However, these effects were not statistically significant. The reducing power of the extracts is increased as the amount of extract is increased. To compare reducing powers of various solvent extracts, 50% ethanol extract showed the highest reducing power. The various solvent extracts were also capable of scavenging nitrite in a manner dependent on concentration. They exhibited scavenging effects 90% on nitrite at the dose 500 p.g of water extract from propolis. The propolis extract significantly inhibited all the microorganisms tested, showing the largest inhibitory zone for Bacillus subtilis and Bacillus cereus. The 70% ethanol extract from propolis exhibited the strongest inhibitory effect on A549. The inhibitory effect of four extracts showed in that order 70% ethanol extract>95% ethanol extract>water extract>50% ethanol extract.

• Korean Journal of Materials Research
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• v.23 no.8
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• pp.453-461
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• 2013

Controlling the stick and slip motions of the contact lines in a confined geometry comprised of a spherical lens with a flat substrate is useful for manufacturing polymer ring patterns. We used a sphere on a flat geometry, by which we could control the interfaces of the solution, vapor and substrate. By this method, hundreds of concentric ring-pattern formations of a linear conjugated polymer, poly [2-methoxy-5-(2-thylhexyloxy)-1,4-phenylenevinylene] (MEH-PPV), were generated with excellent regularity over large areas after complete solvent evaporation. Subsequently, the MEH-PPV ring patterns played a role as a directed template to organize highly regular concentric rings of single-walled carbon nanotubes(SWCNTs); when a droplet of the SWCNT suspension in water was casted onto the prepared substrate, hydrophobic polymer patterns confined the water dispersed SWCNTs in between the hydrophilicized $SiO_2/Si$ substrate. As the solvent evaporated, SWCNT-rings were formed in between MEH-PPV rings with controlled density. Finally, we used a lift-off process to produce SWCNT patterns by the removal of a sacrificial polymer template with organic solvent. We also fabricated a field effect transistor using self-assembled SWCNT networks on a $SiO_2/Si$ substrate.

• Membrane Journal
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• v.20 no.2
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• pp.135-141
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• 2010

Various asymmetric Polysulfone membranes were prepared for a MBR process. Ether-typed alcohols (co-solvent) were added into a dope solution in order to control the pore size of membrane, whose effect on water permeability were investigated. Pore size of the prepared membranes were more affected by molecular-structure of co-solvent than by boiling point of theirs. With the increasing order of methoxy ($CH_3$-O-) < secondary propanol ($-CH_2$-CH(OH)$-CH_3$) < ethoxy ($CH_3-CH_2$-O-), water permeability of the prepared membrane increased. The phenomenon might attribute to the difference of molecularly steric hinderance of co-solvent (eg, Methoxy propanol, Ethoxy ethanol, Methoxy ethanol) in dope solution during the phase inversion. By the addition of ether typed alcohol into a dope solution, the pore size of MF (microfiltration) could be controlled. Also, Membrane prepared was applied to a MBR process and the system was stably operated for 2 months.