• 한국식품영양과학회지
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• 제22권6호
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• pp.771-776
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• 1993

Aqueous extraction of sea tangle at the temperature range of 60~10$0^{\circ}C$ was studied for temperature effects on soluble solids and protein yields, amino nitrogen, turbidity and viscosity of extracts. The solids and protein yield were increased as the temperature increased and most of solids and protein were extracted during 1 hour. The supernatant ratio after centrifugation showed significantly low for the extraction at 6$0^{\circ}C$. More temperature effects were found on turbidity and viscosity than yields. A significantly higher total amino nitrogen contents was obtained from higher temperature at initial stage of extraction and then the differences of them became to be narrow as the extraction prolonged further. The low values of 24.1% solids and 13.5% protein yields after 2 hours of extraction at 10$0^{\circ}C$ indicated that most of solids in sea tangle are insouble.

• 대한수의학회지
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• 제41권2호
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• pp.243-252
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• 2001

Milk constituents and somatic cell count (SCC) were analysed for 4,059 milk samples from 178 dairy farms from April to December in 1999. Correlations among each milk constituents, milk urea (MU) concentration and SCC in association with lactation stage, milk yield and parity, and balancing status of nutrients' supplies were analysed, and the results are summarized as follows; Averages of milk fat percent, total solids percent and milk yield were $3.72{\pm}0.91%$, $12.50{\pm}1.31%$ and $23.80{\pm}8.54kg$, respectively, whereas those were significantly lower during the summer season. In contrast, average of MU concentration was $0.0361{\pm}0.0006%$ which was significantly higher during the summer season. With milk yield, concentrations of fat, protein and SCC in milk decreased but concentrations of lactose and urea in milk and body condition score (BCS) were not altered. Concentrations of fat, protein, lactose, total solids, SNF, and urea in milk were significantly affected by stage of lactation ($P{\leq}0.0001$) but SCC was not changed. Parity of dairy cows had a significant effect on concentrations of fat ($P{\leq}0.02$), lactose ($P{\leq}0.0001$), total solids ($P{\leq}0.002$), and SNF ($P{\leq}0.0001$) in milk and milk yield ($P{\leq}0.0005$) but did not change concentrations of urea and protein in milk. Somatic cell count had significant positive correlationship with percentages of fat, protein and total solids ($P{\leq}0.0001$), respectively, but had negative correlationship with percentages of urea and lactose in milk and milk yield ($P{\leq}0.0001$). Milk urea concentration was negatively correlated with concentrations of protein, fat, total solids, and SNF in milk and milk yield ($P{\leq}0.0001$) and, according to regression analysis using milk urea concentration and SCC, following equation was obtained; $Y(MU)=3.688{\times}10^{-2}-4.04{\times}10^{-7}{\times}X(SCC{\times}1,000)(r^2=0.0038$, $P{\leq}0.0001$). We studied balance between protein and energy supplies to dairy cows in each farm based upon milk urea and protein concentrations, and results showed that 137 of total 178 farms fed cows unbalanced amounts of dietary protein and energy.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1407-1413
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• 2005

An experiment was conducted in order to assess the effect of level of RDP:UDP ratio and level of feeding concentrate on milk yield, milk composition and nutrient utilization in lactating crossbred cattle. Twenty four medium producing (-10 kg/d, 45 to 135 days postpartum) lactating crossbred cows were divided into four groups of six animals each in a 2${\times}$2 factorial completely randomized design. The cows in group 1 were fed concentrate mixture I containing 59:41 RDP:UDP ratio (low UDP) at normal plane (LUDP+NP), in group 2 were fed low UDP ration at 115% of NRC (1989) requirements (LUDP+HP), whereas cows in group 3 were fed concentrate mixture II containing 52:48 RDP:UDP ratio (high UDP) at normal plane (HUDP+NP) and in group 4 were fed high UDP ration at 115% of NRC (1989) requirements (HUDP+HP). Green jowar was fed ad libitum as the sole roughage to all the animals. The experimental feeding trial lasted for 105 days. The total dry matter intake (DMI), DMI/100 kg body weight, DMI/kg $W^{0.75}$, digestibilities of DM, OM, CP, CF, EE and NFE and intakes of TDN and DCP did not differ significantly among the different groups and also due to both UDP level and plane of nutrition and also due to their interaction. The total dry matter intake varied from 145 g in group 1 (LUDP +NP) to 152.57 g/kg $W^{0.75}$ in group 2 (LUDP+HP) diet. However, increase in milk yield with increased UDP level and also with increased plane of nutrition was observed consistently throughout the experimental period. The average milk yield was 7.66, 8.15, 8.64 and 9.35 kg in groups 1, 2, 3 and 4, respectively and there was no significant difference in milk yield among different groups of cows. The overall daily average milk yields in cows fed with low and high UDP diets were 7.91 and 8.99 kg, respectively and at normal and higher plane of feeding the milk yields were 8.15 and 8.75 kg/day, respectively. Thus, there was 13.65% increase in milk yield due to high UDP level and 7.36% due to higher plane of feeding. The daily 4% FCM yields were 9.20 kg for low UDP diet and 10.28 kg for high UDP diet, whereas it was 9.11 kg at normal plane of feeding and 10.37 kg at higher plane of feeding. Fat yields for the corresponding treatment groups were 0.37, 0.43, 0.41 and 0.48 kg, respectively. The 4% FCM yield and also fat yield did not differ significantly among different dietary treatments and also due to UDP level and plane of nutrition, however, 4% FCM yield was increased by 11.74% with high UDP level and 13.83% with higher plane of feeding. The values for total solids, fat, lactose, solids-not-fat and gross energy contents in milk differed significantly (p<0.05) among the different groups and were significantly (p<0.05) higher in milk of cows fed LUDP+HP diet followed by HUDP+HP diet. Total solids (14.65 and 13.83%), lactose (5.44 and 4.92%), solids-not-fat (9.44 and 8.83%) and gross energy (887 and 838 kcal/kg) of milk decreased significantly (p<0.05) with increased UDP level while total solids (13.84 and 14.64), fat (4.84 and 5.36%) and gross energy (832 and 894 kcal/kg) increased significantly (p<0.05) with increase in plane of feeding. Gross and net energetic efficiencies and also gross and net efficiencies of nitrogen utilization for milk production were not significantly different among different groups and also were not affected significantly due to either UDP levels or plane of feeding. Results of the present study suggest that, increasing UDP level from 41% to 48% of CP in concentrate mixture and also increasing plane of feeding from normal (100%) to 115% of NRC requirements maintain a consistently higher milk production.

• Journal of Ginseng Research
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• 제4권1호
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• pp.88-95
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• 1980

Red ginseng was extracted with water and analyzed for yield, saponin, pectin and other chemical composition. It was found that: (1) The total solid content in extract after 6 times of extraction was 46.8%,: including 13.6% of centrifugal residue; (2) 83.7% of total extractable solids and 86% total saponin was extracted after the initial three runs of extraction. (3) No significant changes were observed in HPLC pattern of extracted saponins over a range of extractions; (4) The ratio of centrifugal residue to total solids increased as the number of extractions increased; (5) The ratios of fat, protein, reducing sugar and pectin contents decreased with repeating extraction while those of crude fiber, total sugar and 35% alcohol insoluble residue increased when they were compared with total solids.

• Asian-Australasian Journal of Animal Sciences
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• 제32권4호
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• pp.459-466
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• 2019

Objective: This study assessed the possible existence of genotype by environment interactions for milk, fat and protein yields in Holstein cattle raised in one of the most important milk production basins in Brazil. Methods: Changes in the genetic parameters and breeding values were evaluated for 57,967 animals from three distinct regions of southern Brazil, divided according to differences in climate. The genotype by environment interaction was determined by genetic correlations between regions, estimated by the restricted maximum likelihood, considering the animal model. Bull rankings were investigated to verify the ratio of coincident selected animals between regions for each trait. Results: The estimates of heritability coefficients were similar between two regions, but were lower in the third evaluated area, for all traits. Genetic correlations between regions were high, ranging from 0.91 to 0.99 for milk, fat and protein yields, representing the absence of a genotype by environment interaction for productive traits. The percentage of selection error between regions for the top 10% of animals ranged from 0.88% to 2.07% for milk yield, 0.99% to 2.46% for fat yield and 0.59% to 3.15% for protein yield. Conclusion: A slight change in genotype between areas was expected since no significant genotype by environment interactions were identified, facilitating the process of selecting Holstein cattle in southern Brazil.

• 농업과학연구
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• 제21권1호
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• pp.45-53
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• 1994

본(本) 연구(硏究)는 1990년부터 1991년까지 국립종축원에서 사육한 Holstein종 159두에서 조사된 성적을 근거로 최소자승법을 이용하여 종모우(種牡牛), 분만계절(分娩季節)및 산차(産差)가 비유량(泌乳量)인 유량(乳量) 및 유지방량과 유조성분(乳組成分)인 유지율(乳脂率), 유단백질율(乳蛋白質率), 무지고형분율(無脂固型紛率)및 총고형분율(總固型紛率)에 미치는 효과(效果)를 추정하였던바 그 결과(結果)를 요약하면 다음과 같다. 1. 비유량(泌乳量)에 있어서 유량(乳量)및 유지량(乳脂量)은 각각 $7,26.56{\pm}3,57.24$ 및 $230.95{\pm}43.56Kg$ 이었고, 유조성분(乳組成分)에 있어서는 유지율(乳脂率), 유단백질율(乳蛋白質率), 무지고형분율(無脂固型紛率) 및 총고형분율(總固型紛率)에 있어서 각각 $3.69{\pm}0.43$, $3.32{\pm}0.41$, $9.15{\pm}0.49$및 $12.75{\pm}0.96%$ 이었고, 변이계수는 유량, 유지량, 유지율 및 유단백질율에서 각각 18.68, 17.64, 11.88 및 12.34%이었다. 2. 종모우(種牡牛)의 효과(效果)에 있어서는 유량(乳量)과 유지량(乳脂量)및 유지율(乳脂率)에서 고도의 유의차 (P<0.01)가 인정되었으며, 유단백질율(乳蛋白質率) 및 총고형분율(總固型粉率)에서도 유의차 (P<0.05)가 인정되었다. 모우별(牡牛別)로는 유량(乳量)에서 B, L, 및 O 종모우(種牡牛)가 각각 7,571.22, 7,499.11 및 7,420.58Kg으로 가장 우수하였고, 유단백질율에서는 A, F 및 K 종모우가 각각 3.75, 3.64 및 3.65%로 다른 종모우에 비하여 높았다. 3. 산차(産差)의 효과(效果)에 있어서 유량(乳量)에서 고도의 유의차 (P<0.01)가 인정되었으며, 유지방량 및 유지율(乳脂率)에서는 유의차(P<0.05)가 인정되었고, 산차별(産差別)로는 유량(乳量)과 유지방량에서 3산차(産差)가 각각 7,634.54 및 274.98Kg로 가장 우수하였고, 유지율 및 유단백질율에서는 4산차 이상이 각각 3.90, 3.50%로 다른산차에 비하여 높았다. 4. 분만계절(分娩季節)의 효과(效果)에 있어서는 유량(乳量)에서 고도의 유의차 (P<0.01)가 인정되었으며 유지량(乳脂量), 유지율(乳脂率), 유단백질율(乳蛋白質率), 및 총고형분율(總固型紛率)에서 유의차(P<0.05)가 인정되었으며, 분만계절별(分娩季節別)로는 유량(乳量)에 있어서 봄과 겨울이 각각 7,310.31 및 7,364.57Kg로 우수하였고, 유단백질율에 있어서는 봄과 가을이 각각 3.68 및 3.52%로 우수하였다.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1116-1123
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• 2020

Velvet antler (VA) is a historically traditional medicinal supplement and is well known in Asian countries for its pharmaceutical and health benefits. The objectives for this study were to optimize the hot water extraction (HWE) of VA for the Korean VA industry, and to determine the most effective pretreatment method among microwave (MW), ultrasonication (US), and enzymatic (EZ) techniques. Using response surface methodology, optimum extraction temperatures and times were determined by central composite design configuration based on extraction yield and sialic acid content. Various quality parameters of VA extract including yield, soluble solid, protein, and sialic acid contents were also compared with the conjunction of HWE and pretreatment. The yield and sialic acid content of VA extract were determined to be 40% and 0.73 mg/g, respectively, under an optimum temperature of 100℃ at 24 h of extraction time. The yields from VA extracts pretreated with MW, US, and EZ were 17.42%, 19.73%, and 29.15%, respectively. Among the tested commercial enzymes, pepsin was the most effective proteolytic enzyme and led to the highest yield (47.65%), soluble solids (4.03 °brix), protein (1.12 mg/ml), and sialic acid (3.04 mg/ml) contents from VA extract.

• 한국초지조사료학회지
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• 제33권3호
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• pp.213-218
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• 2013

This study was conducted to investigate the effects of supplementing additional sucrose, in the form of black sugar (BS), into the diet of Holstein dairy cows on dry matter intake (DMI), milk yield, and milk composition. Eight Holstein dairy cows ($741{\pm}65.8kg$ body weight) were divided into two groups, including the control and BS groups. Animals in the control group were offered a total mixed ration (TMR) ad libitum, and the BS group was offered TMR with 300 g of BS/head/d. After two weeks of adaptation period, the animal performance, including DMI, milk yield and milk composition, was measured. Cows supplemented with BS appeared to consume more feed than that by the controls (i.e., 17.08 and 18.28 kg/d for the control and BS groups, respectively). However, there were no significant differences between treatments. Milk yield or milk composition, such as milk fat, milk protein, lactose, solids-non-fat, total solids and pH, did not differ between treatments. However, there was a significant difference (p<0.05) in the concentration of milk urea nitrogen (MUN). The MUN concentration of the BS group was approximately 15% lower than that of the control group (i.e., 18.75 vs. 16.05 mg/dL for the control and BS groups, respectively), which suggests improved nitrogen metabolism in the animals. The somatic cell count was numerically lower in the cows of the BS group compared to those in the control group. However, a significant difference was not noted due to the substantial amount of variation among cows. In terms of the trace mineral composition for milk, the concentration of Cu from BS animals was higher (p<0.05) than that of the control animals. In summary, supplementing the diets of dairy cows with BS marginally affected animal performance and improved nitrogen metabolism. The level of supplementation and other factors, such as animal variation were discussed.

• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.21-25
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• 1990

Fructo-oligosaccharides are found in many plants, such as onion, burdock and wheat. They are not well hydrolyzed by digestive enzymes in animals, but are peculiarly assimilated by Bifidobacterium and some useful bacteria. In our previous experiment (Kobayashi et al., 1987) it was suggested that they were effective in decreasing energy loss in the metabolism of dairy calves. In the present study, the effects of fructo-oligosaccharides on body weight, milk-yield and milk-components (fat, protein and solids-not-fat) were investigated in dairy cows. Lactating cows were fed a standard diet containing fructoligo saccharides at 18.70g, 9.35g and 0.0g (control) per 100kg body weight, day for three weeks. Neither treatments significantly affected any of the parameters examined. The fructo-oligosaccharides were assumed to be hydrolyzed by rumen microorganisms and hardly to affect the bacterium florae in the intestines of the lactating cows.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1449-1453
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• 2015

The objective of the present study was to determine the effects of feeding pelletized broccoli byproducts (PBB) on milk yield and milk composition in dairy cows. In Trial 1, an in vitro gas test determined the optimal replacement level of PBB in a concentrate mixture in a mixed substrate with Chinese wild ryegrass hay (50:50, w/w) at levels of 0, 10%, 20%, 30%, or 40% (dry matter basis). When the concentrate was replaced by PBB at a level of 20%, no adverse effects were found on the gas volume or its rate constant during ruminal fermentation. In trial 2, 24 lactating cows (days in milk = $170.4{\pm}35$; milk yield = $30{\pm}3kg/d$; body weight = $580{\pm}13kg$) were divided into 12 blocks based on day in milk and milk yield and randomly allocated to two dietary treatments: a basic diet with or without PBB replacing 20% of the concentrate mixture. The feeding trial lasted for 56 days; the first week allowed for adaptation to the diet. The milk composition was analyzed once a week. No significant difference in milk yield was observed between the two groups (23.5 vs 24.2 kg). A significant increase was found in milk fat content in the PBB group (p<0.05). Inclusion of PBB did not affect milk protein, lactose, total solids or solids-not-fat (p>0.05). These results indicated that PBB could be included in dairy cattle diets at a suitable level to replace concentrate mixture without any adverse effects on dairy performance.