• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.3
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• pp.566-570
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• 2004

Cultivation conditions for overproduction of lovastatins were investigated from the lovastatin producing strain N-03 which was obtained with NTG (N-methyl-N'-nitro-nitrosoguanidine) treatment from Aspergiliu ferrous ATCC 20542. Produced lactone and acid form of lovastatin were detected, and analyzed by HPLC method. In liquid culture, medium No. 2 containing soy protein produced higher amounts of the lovastatins than medium No. 1 (contained rapeseed oil). In solid culture, maximum production was obtained at 28$^{\circ}C$ for 15 days cultivation using cooked wheat bran. For the overproduction of lovastatin from this strain, solid culture method using plastic bag is more superior than liquid culture.

• Structural Engineering and Mechanics
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• v.66 no.1
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• pp.113-124
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• 2018

In the present paper, we have considered a layered medium of two semi-infinite nonlocal elastic solids with intermediate transversely isotropic magnetothermoelastic solid. The intermediate slab is of uniform thickness with the effects of two temperature, rotation and Hall current and with and without energy dissipation. A plane longitudinal or transverse wave propagating through one of the nonlocal elastic solid half spaces, is made incident upon transversely isotropic slab and it results into various reflected and refracted waves. The amplitude ratios of various reflected and refracted waves are obtained by using appropriate boundary conditions. The effect of nonlocal parameter on the variation of various amplitude ratios with angle of incidence are depicted graphically. Some cases of interest are also deduced.

• Journal of Microbiology and Biotechnology
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• v.19 no.5
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• pp.462-467
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• 2009

Cyclosporin A (CyA) produced by Tolypocladium inflatum is a promising drug owing to its immunosuppressive and antifungal activities. From an industrial point of view, the necessity to obtain a suitable and economic medium for higher production of CyA was the aim of this work. The present study evaluated the effect of different fermentation parameters in solid state fermentation, such as selection of solid substrate, hydrolysis of substrates, initial moisture content, supplementation of salts, additional carbon, and nitrogen sources, as well as the inoculum age and size, on production of CyA by Tolypocladium inflatum MTCC 557. The fermentation was carried out at $25{\pm}2^{\circ}C$ for 9 days. A combination of hydrolyzed wheat bran flour and coconut oil cake (1:1) at 70% initial moisture content supported a maximum production of $3,872{\pm}156\;mg$ CyA/kg substrate as compared with $792{\pm}33\;mg/kg$ substrate before optimization. Furthermore, supplementation of salts, glycerol (1% w/w), and ammonium sulfate (1% w/w) increased the production of CyA to $5,454{\pm}75\;mg/kg$ substrate. Inoculation of 5 g of solid substrate with 6 ml of 72-h-old seed culture resulted in a maximum production of $6,480{\pm}95\;mg$ CyA/kg substrate.

• Journal of Plant Biology
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• v.36 no.3
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• pp.211-218
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• 1993

Plant regeneration was accomplished from protoplast culture of rice (Oryza sativa L. cv. Taebaeg). Embryogenic callus was induced from mature seed on MS medium containing 5 mM proline, 2.5 mg/L 2,4-D, 30 g/L sucrose in the dark at 28$^{\circ}C$ and used to establish embryogenic cell suspension culture. Suspension cells were subcultured every one week in N6 medium supplemented with 5 mM proline, 200 mg/L casein hydrolysate, 2.5 mg/L 2,4-D and amino acids of AA medium. Suspension cultures were composed of cells that were densely cytoplasmic, potentially embryogenic and were at least maintained for more than 6 months in liquid medium. Protoplasts were isolated from fast-growing suspension culture cells and cultured in a slightly modified KpR medium by mixed nurse culture. Isolated protoplasts began to divide within 5~7 days and thereafter, protoplast-derived calli were sequentially transferred to callus proliferating medium that soft agar MS medium contained 2 mg/L 2,4-D and produced distinct embryogenic cells. Microcolonies were then transferred to solid medium which consisted of MS medium containing 5 mg/L kinetin, 1 mg/L NAA, 1 mg/L ABA, 30 g/L sucrose and 10 g/L sorbitol under fluorescent light. Mulitple shoots of 4~5 per callus emerged and were transferred to hormone-free MS medium for root initiation. Thereafter, The plantlets were transferred to pots of soil to mature in the culture room.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.96-99
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• 1995

This experiment was carried out to analyze the effect of indole on the synthesis of indirubin in suspension culture of Polygonum tinctorium. Adding indole and L-tryptophan into culture media was re­vealed that indirubin was synthesized in callus grown on solid medium containing indole and proper concentration of indole for indirubin production was decided as 200mg/1. Indirubin content in suspension culture was higher than in solid medium with considerable amount of indirubin secresed into media in suspension culture and highest quantity of indirubin was obtained when indole was added into medium after 20 days suspension culture.

• Korean Journal of Plant Resources
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• v.24 no.3
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• pp.280-285
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• 2011

In this study, we established a novel somatic embryogenesis and plant regeneration system through cell suspension culture of white dandelion (Taraxacum coreanum NAKAI.). Embryogenic calli could be initiated from leaf and root explants of sterile seedlings on solid Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) after 3-week cultures. To proliferate embryogenic calli rapidly, cell suspension culture was performed with transferred to liquid MS medium with various combinations of plant growth regulators (PGRs) including 2,4-D, ${\alpha}$-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), $N^6$-benzylamino purine (BAP), thidiazuron (TDZ), and kinetin. During suspension cultures, embryogenic calli not only greatly proliferated, but shoot organogenesis also simultaneously occurred from the surface of somatic embryos. Among them, TDZ at lower concentration, 0.1 mg/L produced the highest efficiency of somatic embryo formation and shoot organogenesis. Rooting of embryogenic calli with adventitious shoots was done on solid MS medium containing 0.1 mg/L NAA and 0.3% activated carbon. Nearly 80% of embryogenic calli with shoot organogenesis could be rooted normal. Well-rooted plantlets were transferred into pots under a greenhouse condition, and plants derived from this system appeared phenotypically normal.

• Geomechanics and Engineering
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• v.14 no.1
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• pp.19-27
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• 2018

It follows from the basic principles of mechanics of deformable solids relating to the strength, stability and propagation of elastic waves that the Earth's inner core cannot exist in the form of a spherical structure in the assumed thermobaric conditions and calculation values of physico-mechanical parameters. Pressure level reaches a value that is significantly greater than the theoretical limit of medium strength in the model approximations at the surface of the sphere of the inner core. On the other hand, equilibrium state of the sphere is unstable on the geometric forming at much lower loads under the influence of the "dead" surface loads. In case of the action of "follower" loads, the assumed pressure value on the surface of the sphere is comparable with the value of the critical load of "internal" instability. In these cases, due to the instability of the equilibrium state, propagation of homogeneous deformations becomes uneven in the sphere. Moreover, the elastic waves with actual velocity cannot propagate in such conditions in solid medium. Violation of these fundamental conditions of mechanics required in determining the physical and mechanical properties of the medium should be taken into account in the integrated interpretations of seismic and laboratory (experimental) data. In this case, application of the linear theory of elasticity and elastic waves does not ensure the reliability of results on the structure and composition of the Earth's core despite compliance with the required integral conditions on the mass, moment of inertia and natural oscillations of the Earth.

• Current Optics and Photonics
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• v.6 no.5
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• pp.479-488
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• 2022

To account for the internal thermal effects of solid-state lasers, a method using a back propagation (BP) neural network integrated with a particle swarm optimization (PSO) algorithm is developed, which is a new wavefront distortion correction technique. In particular, by using a slab laser model, a series of fiber pumped sources are employed to form a controlled array to pump the gain medium, allowing the internal temperature field of the gain medium to be designed by altering the power of each pump source. Furthermore, the BP artificial neural network is employed to construct a nonlinear mapping relationship between the power matrix of the pump array and the thermally induced wavefront aberration. Lastly, the suppression of thermally induced wavefront distortion can be achieved by changing the power matrix of the pump array and obtaining the optimal pump light intensity distribution combined using the PSO algorithm. The minimal beam quality β can be obtained by optimally distributing the pumping light. Compared with the method of designing uniform pumping light into the gain medium, the theoretically computed single pass beam quality β value is optimized from 5.34 to 1.28. In this numerical analysis, experiments are conducted to validate the relationship between the thermally generated wavefront and certain pumping light distributions.

• Transactions of the Korean Society of Mechanical Engineers
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• v.17 no.10
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• pp.2582-2589
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• 1993

Transient heat transfer characteristics of cooling of a spherical body were investigated in the radiatively active spherical medium. Initially the spherical body and the medium were maintained at their constant temperatures. Then heat transfer begins from spherical body t medium. The heat transfer mode inside the spherical body is just conduction. But heat is transferred by both conduction and radiation inside the medium. All thermodynamic properties were held constant in time. Spherical symmetry is assumed. DOM was adopted to solve RTE. The effect of characteries-tic optical thickness, conduction to radiation parameters, and solid surface emissivity has been studied.

• 한국정보디스플레이학회:학술대회논문집
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• 2007.08b
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• pp.1781-1784
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• 2007

The increase of repetition rate, the dithering of laser optics, and the extension of pulse duration time are major approaches in improving the picture quality of AMOLEDs fabricated by excimer laser crystallization (ELC). Advanced solid phase crystallization (ASPC) has been developed to improve the uniformity and the process cost. Even though the mobility of ASPC-TFT is lower than that of ELC-TFT, it is high enough to drive AMOLED pixels.