• Journal of Plant Biotechnology
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• v.32 no.2
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• pp.129-134
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• 2005

Effective micropropagation system via somatic embryognesis was established for a Phytophthora resistant Aralia elata cultivar. Different kinds of growth regulators were needed to induce embryogenic callus with different explant sources. When leaf explants were used, a combination of 2,4-D, TDZ and L-glutamine was needed, whereas when petiole and root explants needed only 1.0 mg/L 2,4-D. Embryogenic callus induction rate under the optimum culture condition was 75.0%, 67.0% and 83.0% from leaf, petiole and root segment, respectively. Somatic embryo germination and plantlet conversion rate appeared to be influenced greatly by various osmoticums. More than 90% of embryos germinated when treated with sucrose, glucose and maltose. However, the highest conversion rate (72%) was recorded on medium with 2% sucrose only. The converted plantlets grew normally on 1/2MS basal medium, were acclimatized on artificial soil mixture and survived more than 95% in the greenhouse condition. The results suggest that the species can be clonally propagated through in vitro culture system via somatic embryogenesis.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.806-811
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• 2007

Bacillus anthracis is a soil pathogen capable of causing anthrax that is closely related to several environmental species, including B. cereus, B. mycoides, and B. thuringiensis. DNA homology studies showed that B. anthracis, B. cereus, B. mycoides, and B. thuringiensis are closely related, with a high sequence homology. To establish a method to specifically detect B. anthracis in situations such as environmental contamination, we initially performed RAPD-PCR with a 10-mer random primer and confirmed the presence of specific PCR bands only in B. anthracis species. One region specific for B. anthracis was cloned and sequenced, and an internal primer set was designed to amplify a 241-bp DNA fragment within the sequenced region. The PCR system involving these specific primer sets has practical applications. Using lyses methods to prepare the samples for PCR, it was possible to quickly amplify the 241-bp DNA segment from samples containing only a few bacteria. Thus, the PCR detection method developed in this study is expected to facilitate the monitoring of environmental B. anthracis contamination.

• Korean Journal of Soil Science and Fertilizer
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• v.12 no.2
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• pp.77-82
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• 1979

Wilting and transpiration charactistics of Panax ginseng leaves were investigated at two temperature levels. Water potential and water absorption characteristics of leaf segments were also observed. Soybean leaves were compared. 1. Ginseng leaves were thiner, higher in water content and lower in dehydration rate. But time required to reach permanent wilting point (pwp) was less than half of that of soybean leaves because water content at pwp was about two times higher (80% of initial water for ginseng and 50% for soybean leaves). The time was shorter under high air temperature. 2. Transpiration rate was about a quater of soybean leaves and lower at $33^{\circ}C$ than $23^{\circ}C$, indicating that ginseng leaves are less tolorant to high air temperature. 3. Ginseng leaf segment showed smaller water free space but greater water deficit and little difference in was absorption rate. 4. Water potential of leaves measured by liquid immersion method was lower than that of soybean leaves. 5. Above results strongly suggest that ginseng plants are more susceptible to water stress. Thus greater light intensity during leaf growing stage (April to June) is recommendable to increase stomate frequency resulting greater transpiration rate and high temperature tolerance during July and August. Abundant water around roots seems to be beneficial as long as oxygen is not limited in rhizosphere.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.2
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• pp.513-525
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• 2018

A 3 dimensional numerical analysis for shield TBM tunnel should take into account various characteristics of the shield TBM excavation, such as gap, tail void, segment installation, and backfill injection. However, analysis method considering excavation characteristics are generally mixed with various method, resulting in concern of consistency and reliability degradation of the analytical results. In this paper, a parametric study is carried out by using actually measured ground settlement data on various methods that can be used for 3 dimensional numerical analysis of shield TBM tunneling. As a result, we have analyzed and arranged an analytical method to predict similarly the behavior of ground settlement and tunnel face pressure at the design stage. Skin plate pressure, backfill pressure and soil model have been identified as the most significant influences on the ground settlement. The grout pressure model is considered to be applicable when there is no volume loss information on the excavated ground, such as seabed tunnels, or when it is important to identify the behavior around a tunnel, such as surface settlement as well as face pressure. And it is considered that designers can use these guidelines as a base material to perform a reasonable 3 dimensional numerical analysis that reflects the ground conditions and the features of the shield TBM tunneling.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.4
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• pp.259-265
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• 2004

A quicker method was developed for foliar analysis in diagnosis of nitrogen in apple trees based on multivariate calibration procedure using partial least squares regression (PLSR) and principal component regression (PCR) to establish the relationship between reflectance spectra in the near infrared region and nitrogen content of fresh- and dry-leaf. Several spectral pre-processing methods such as smoothing, mean normalization, multiplicative scatter correction (MSC) and derivatives were used to improve the robustness and performance of the calibration models. Norris first derivative with a seven point segment and a gap of six points on MSC gave the best result of partial least squares-1 PLS-1) model for dry-leaf samples with root mean square error of prediction (RMSEP) equal to $0.699g\;kg^{-1}$, and that the Savitzky-Golay first derivate with a seven point convolution and a quadratic polynomial on MSC gave the best results of PLS-1 model for fresh-samples with RMSEP of $1.202g\;kg^{-1}$. The best PCR model was obtained with Savitzky-Golay first derivative using a seven point convolution and a quadratic polynomial on mean normalization for dry leaf samples with RMSEP of $0.553g\;kg^{-1}$, and obtained with the Savitzky-Golay first derivate using a seven point convolution and a quadratic polynomial for fresh samples with RMSEP of $1.047g\;kg^{-1}$. The results indicate that nitrogen can be determined by the near infrared reflectance (NIR) technology for fresh- and dry-leaf of apple.

• Journal of Korea Foresty Energy
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• v.7 no.1
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• pp.11-21
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• 1987

To estimate the biomass, net production and nutrient distribution of Pinus Koraiensis and Quercus mongolica stand, experimental plots located in Kwangju of Kyonggi-do were selected. Ten sample trees selected account of DBH distribution were felled and the diagram of oven-dry weight distrubution of leaves, branches and stems for each 1m segment was constructed. The logarithmic regression equations between dry weight of each component and the variable of $(DBH)^2$ H obtained. The standing crops were estimated to be as much as 152.07 and 156.02 tons of dry matter per hectare in P. koraiensis and Q. mongolica stand respectively. The net production was estimated as 24.66 and 20.35t/ha$\cdot$yr and the net assimilation rate was 1.90 and 3.42kg/kg$\cdot$yr in same order. Particulary large proportions of nitrogen. calcium and magnesium were found in soil of P. koraiensis stand, phosphours and potassium in overstory of it. At Q. mongolica stand, large proportions of nitrogen. phosphorus. potassium and magnesium were found in soil and calcium in overstory. The amounts of litter fall was 4.013t/ha$\cdot$8 months in P. koraiensis stand and 3.490t/ha$\cdot$8 months in Q. mongolica stand.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.5
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• pp.1-12
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• 2019

It is important to measure the height of trees as an essential element for assessing the forest health in urban areas. Therefore, an automated method that can measure the height of individual tree as a three-dimensional forest information is needed in an extensive and dense forest. Since airborne LiDAR dataset is easy to analyze the tree height(z-coordinate) of forests, studies on individual tree height measurement could be performed as an assessment forest health. Especially in urban forests, that adversely affected by habitat fragmentation and isolation. So this study was analyzed to measure the height of individual trees for assessing the urban forests health, Furthermore to identify environmental factors that affect forest growth. The survey was conducted in the Mt. Bongseo located in Seobuk-gu. Cheonan-si(Middle Chungcheong Province). We segment the individual trees on coniferous by automatic method using the airborne LiDAR dataset of the two periods (year of 2016 and 2017) and to find out individual tree growth. Segmentation of individual trees was performed by using the watershed algorithm and the local maximum, and the tree growth was determined by the difference of the tree height according to the two periods. After we clarify the relationship between the environmental factors affecting the tree growth. The tree growth of Mt. Bongseo was about 20cm for a year, and it was analyzed to be lower than 23.9cm/year of the growth of the dominant species, Pinus rigida. This may have an adverse effect on the growth of isolated urban forests. It also determined different trees growth according to age, diameter and density class in the stock map, effective soil depth and drainage grade in the soil map. There was a statistically significant positive correlation between the distance to the road and the solar radiation as an environmental factor affecting the tree growth. Since there is less correlation, it is necessary to determine other influencing factors affecting tree growth in urban forests besides anthropogenic influences. This study is the first data for the analysis of segmentation and the growth of the individual tree, and it can be used as a scientific data of the urban forest health assessment and management.

• Journal of the Korean Geotechnical Society
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• v.38 no.11
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• pp.69-86
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• 2022

The railway is widely used to transport passengers and freight due to its punctuality and large transport capacity. The recent remarkable development in construction technology enables various subsea railway tunnels for continent-continent or continent-island connectivity. In Korea, design and construction experience is primarily based on the successful completion of the Boryeong subsea tunnel (2021) and the Gadeok subsea tunnel (2010). However, frequent earthquakes with diverse magnitudes, globally induced and continuously increased the awareness of seismic risks and the frequency of domestic earthquakes. The effect of an earthquake on the subsea tunnel is very complicated. However, ground conditions and seismic waves are considered the main factors. This study simulated four ground types of 3-dimensional numerical models, such as soil, rock, composite, and fractured zone, to analyze the effect of ground type and seismic wave. A virtual subsea railway shield tunnel considering external water pressure was modeled. Further, three different seismic waves with long-term, short-term, and both periods were studied. The dynamic analyses by finite difference method were performed to investigate the displacement and stress characteristics. Consequently, the long-term period wave exhibited a predominant lateral displacement response in soil and the short-term period wave in rock. The artificial wave, which had both periodic characteristics, demonstrated predominant in the fractured zone. The effect of an earthquake is more noticeable in the stress of the tunnel segment than in displacement because of confining effect of ground and structural elements in the shield tunnel. 

• Journal of Korean Tunnelling and Underground Space Association
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• v.23 no.4
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• pp.233-252
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• 2021

This paper is a study to improve the efficiency of mixing technology in the shield TBM chamber. Currently, the number of construction cases using the TBM method is increasing in Korea. According to the increasing use of TBM method, research on TBM method such as Disc Cutter, Cutter bit, and Segment also shows an increasing trend. However, there is little research on the mixing efficiency in chamber and chamber. In order to improve the smooth soil treatment and the behavior of the excavated soil, a study was conducted on the change of the mixing efficiency according to the effective mixing bar arrangement in the chamber. In the scale model experiment, the ground was composed using plastic materials of different colors for ease of identification. In addition, the mixing bar arrangement was different and classified into 4 cases, and the particle size distribution was classified into single particle size and multiple particle size, and the experiment was conducted with a total of 8 cases. The rotation speed of the cutter head of all cases was the same as 5 RPM, and the experiment time was also carried out in the same condition, 1 minute and 30 seconds. In order to check the mixing efficiency, samples at the upper, middle (left or right), and lower positions of each case were collected and analyzed. As a result of the scaled-down model experiment, the mixing efficiency of Case 4 and Case 4-1 increased compared to Case 1 and Case 1-1, which are actually used. Accordingly, it is expected that the mixing efficiency can be increased by changing the arrangement of the mixing bar in the chamber, and it is considered to be effective in saving air as the mixing efficiency increases. Therefore, this study is considered to be an important indicator for the use of shield TBM in Korea.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.10a
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• pp.449-456
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• 1999

The concrete wall is the most useful of retaining structure which can obtain the engineering stability, but has problems that is not friendly with nature environment in a fine view, such as poor rear drainage, and shrinkage crack by temperature difference, etc. Because of this problems, the research for a segmental crib retaining wall has been performed. A segmental crib retaining wall is quickly and easily erected because is possible to be erected as the individual members, and is not sensitive to differential settlement and earthquakes. Also, it shows effective drainage and has a friendly advantage with nature environment because of being able to be planted with vines and shrubs in retaining walls The design of crib retaining walls has traditionally been based on classical soil mechanics theories. These theories, originally derived by Rankine(1857) and Coulomb(1776), assume that the wall acts as a rigid body. This assumption results in failure being predicted by either monolithic overturning or base sliding mechanisms. However, the wall consists of individual members which have been created a three dimensional grid. This grid confines an fill mass which becomes part of the wall. The filled wall resists the earth pressure with the same mechanism of classical gravity walls. Because of the flexibility of the individual segment, it allows relative movement between the individual members within the wall. The three dimensional flexible grid leads to stress redistribution when the wall is subjected to external or fill loads. Due to the flexibility and the stress redistribution, the failure of segmental crib wall consists of not only overturing and base sliding but the local deformation and the failure between the segmental members. It has been researched in the field that due to this flexibility and load redistribution, serviceability failure of segmental crib walls is unlikely to be due to overturning or base sliding. Therefore, in this study, the relative displacement appearance of retaining wall due to variation of inclination is measured to examine this behavior characteristics. Also, the behavior characteristics of retaining walls by surcharge load, and location of acting point of retaining wall rear, and the displacement characteristics and deflections are estimated about the existence and nonexistence of Rear Stretcher performing an role in transmitting earth pressure of Header and Stretcher organizing retaining walls. This research focuses on the characteristics due to the behavior of retaining walls. This research focuses on the characteristics due to the behavior of retaining walls.