• Journal of the Korea Organic Resources Recycling Association
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• v.1 no.2
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• pp.259-266
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• 1993

This experiment was carried out to the effect of population densities on the growth reproduction, cast production of the earthworm and chemical composition of worm casts. The population densities of 50, 100, 150, 200 and 250 individuals of the earthworm fed with cow manure was studied in rearing box($6,400cm^3$) and fertility stage during a period of 60 days. The results were summarized as follows 1. Survival rates(SR) of the earthworms had significantly positive correlated with increasing rate(IR). But, survival rates(SR) and increasing rate(IR) had significantly negative correlated with reproductive efficiency(RE). 2. The ranges of optimum population densities estimated were $42.7cm^3-128.0cm^3/worm$. 3. The cast production estimated were 36.5mg-80.9mg/day/worm grown in optimum population densities(50-150 worms/$6,400cm^3$). 4. The earthworm castings are an excellent soil conditioning material with a high chemical composition and their physical properties.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.57-62
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• 2005

Salt-tolerant transgenic Panax ginseng plants were produced by introducing the SAL1 geue (3'(2'), 5'-bis-phosphate nucleotidase) that confers tolerance to the salts through Agrobacterium tumefaciens co-cultivation. Cotyledon explants of immature ginseng zygotic embryos cultured on Murashige and Skoog medium lacking growth regulators formed somatic embryos directly with below 10%, but the 74% tranformation rate were observed at the treatment of phytohormone with 1.0 mg/l 2,4-D and 0.5 mg/l kinetin. Somatic embryos were initially cultured on MS medium supplemented with 250 mg/l cefotaxime for 3 weeks and subsequently subcultured five times to a medium containing 100 mg/l kanamycin and 250 mg/l cefotaxime. Upon development into the cotyledonary stage, these somatic embryos were transferred to on the medium containing 50 mg/l kanamycin and 10 mg/l gibberellic acid to induce germination and strong selection. Integration of the transgene into the plants was confirmed by polymerase chain reaction with specific primers. The ginseng transformants with well-developed shoots and roots were successfully acclimatized in a greenhouse when they were planted in soil.

• Journal of Species Research
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• v.7 no.1
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• pp.50-59
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• 2018

During the course of investigation of indigenous prokaryotic species in Korea, a total of 30 bacterial strains belonging to the phylum Firmicutes were isolated from diverse environmental sites such as soil, avian feces, wastewater treatment plants, fermented vegetables, seawater, algae, sea cucumber, octopus and tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences revealed that each strain showed high sequence similarity (${\geq}98.7%$) to the closest type strain and formed a robust phylogenetic clade with the most closely related species in the phylum Firmicutes. To date, there is no official record of these 30 species in Korea. Therefore, we report 26 species of 12 genera in the order Bacillales and 4 species of 4 genera in the order Lactobacillales which have not been reported in Korea. Morphological and biochemical characteristics, isolation sources and NIBR deposit numbers are described in the species descriptions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.5
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• pp.434-442
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• 1990

Aspergillus sp. CC-29 ws selected for its strong protease activity among various stains of molds found in soil. It was found that the production of alkaline protease reached to maximum when the wheat bran medium containing glucose as carbon source had been cultured for 4 days. Alkaline proteased was purified 36.10 fold from Aspergillus sp. CC-29 The purification procedures included ammonium sulfate fractunation gel filteration on Sepha-dex G-75 G-150 and DEAE-cellulose ion-exchange chromatography, The yield of the purified enzyme was 22.40% The purified enzyme was confirmed as a single band by the polyacryla-mide. When the purified enzyme was applied to SDS-PAGE the molecular weight was estima-ted 24000. The optimum pH for the enzyme activity was 9.0 and the optimum temperature was 4$0^{\circ}C$ The reaction of this enzyme followed typical Michaelis-Menten kinetics with the Km value of 2.10$\times$10-4M with the Vmax of 29.41 $\mu$g/min. The enzyme was reactively stable in alkalic condition and unstable by heat treatment. The activity of alkaline protease was increased by the addition of Ca2+ whereas it was inhibited by Hg2+ Zn2+ at concentration of 1$\times$10-3M.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.4
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• pp.273-278
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• 2002

An efficient low-input system (LIS) for fertiliser use in rice cultivation is necessary to reduce fossil energy use and pollution. Japanese people like Japonica rice, especially cv. Koshihikari. However, it has very low lodging resistance in Japanese weather condition. Our objective was to develop a LIS with the minimum sacrifice of grain yield in rice. Koshihikari was grown using conventional fertilization as a control (CON) with 4 g N $m^{-2}$., 8g $P_2$ $O_{5}$ $m^{-2}$ and 8 g $K_2$O $m^{-2}$ as a basal fertilizer dressing. It was compared with a low fertilizer treatment (LF) with only 4 g $P_2$ $O_{5}$ $m^{-2}$ as a basal dressing in the first year and no basal phosphorus fertilizer in the second year. Chopped rice straw was incorporated into the soil before the cropping season in both years. Fertilizer of 4 g N $m^{-2}$ was top-dressed at 15 days before heading in CON plots and 30 days before heading in LF plots in both years. Lodging was significantly less in LF than in CON plots, however, no rice straw effect was found in low fertilized condition. Grain yields in LF plots were reduced by 15-16% below those of CON plots. Lower yields in LF plots were associated with a reduced number of spikelets per unit area. However, big spikelet size was acquired in LF by 10 days earlier N top dressing than CON plots. A close relationship was found between spikelet numbers and N content of the plant at heading, and between grain yield or shoot dry weight and N content of the plant at maturity. Regardless of the fertilizer application methods, N use efficiency for the number of spikelets, final total dry matter and grain yield was essentially identical among fertilizer treatments. The reduced growth and yield in the LF plots resulted from low absorption of nitrogen. Conclusively, LIS can drastically reduce chemical fertilizer use and facilitate harvest operations by reducing lodging with some yield reduction..

• Journal of Korean Society of Environmental Engineers
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• v.36 no.11
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• pp.733-738
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• 2014

Within the scope that does not interfere with the performance of basic duty, the military has been actively supporting the national policy for the conservation of the natural environment in accordance with the "military environmental protection guidelines". Since there is a lack of information regarding inhabitation for wild fauna and flora, field investigations of the natural environment at Jinhae local unit were performed three times in 2003. As a result of the investigation, lycoris sanguinea as flora, marten, otter, and leopard cat as mammalia, and accipiter soloensis, accipiter gentilis schvedow, accipiter gularis, buteo hemilasius, falco tinnunculus and terpsiphone atrocaudata as bird were confirmed to inhabit. For the conservation of the natural ecosystem, the expansion of research for natural ecosystems within the military unit is required. Additionally, environmental pollution can be prevented through the proper maintenance of basic environmental facilities including sewage treatment facilities, soil pollution control facilities, and waste storage facilities. In cooperation with local governments and private organizations, the natural cleansing action and sustainable wildlife conservation should be expanded.

• Economic and Environmental Geology
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• v.38 no.3 s.172
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• pp.247-260
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• 2005

Extractable concentrations (0.1 N & 1.0 N HCI) of heavy metals in roadside sediments are lower than guidelines for soil recommended by Ministry of Environment. Heavy traffic areas (such as No. 7 national road) show high contents of heavy metals, especially, at curved areas, gully pot, crackdown areas on overspeed, pedestrian crossing etc. Fine fractions $(<63\;{\mu}m)$ of roadside sediments have the highest concentrations of heavy metals, but mass loadings of heavy metal are determined by coarse fractions $(>100{\mu}m)$, due to washing out of fine fraction sediment by runoff water. Proper treatment facilities are needed to control the inflow of fine roadside sediments from No. 7 national road and bridge such as Hanmul bridge.

• Analytical Science and Technology
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• v.23 no.5
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• pp.477-484
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• 2010

Personal care products are a diverse group of synthetic organic chemicals such as antimicrobial compounds, UV filters and organo-phosphate flame retardants and derived from individual usages of soaps, toothpaste and cosmetics. It has been detected in municipal sewage effluent and various environmental samples such as surface water, marine, soil, sediment and aquatic biota in many countries. The occurrence of personal care products in environmental samples could negatively impact the health of the ecosystem and humans, due to persistent, long-term chronic exposure of aquatic organisms. In this study, fifteen personal care products in aquatic environmental samples were determined by gas chromatography-mass spectrometry (GC-MS) with liquidliquid extraction (LLE). Method detection limits were in the range of $0.004\sim0.273\;{\mu}g/L$. Two compounds (TCEP, TCPP) were detected in surface waters and seven compounds (triclosan, 4-MBC, EHMC, BP-3, TCEP, TPP, TBEP) were detected in sewage treatment plants (STP) influents or effluents.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.169-177
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• 1992

An alkaline protease producing microorganism was isolated from soil and identified as Streptomyces griseus HC-1141. The optimum culture condition of Streptomyces griseus HC- 1141 for the production of alkaline protease was as follows; 0.5% casein, 0.05% ammonium chloride, 0.1% ferrous sulfate. 2.0% lactose, pH 8.0 and 84 hrs. The enzyme was purified about 53 folds by ammonium sulfate treatment, DEAE-cellulose ion exchange chromatography and gel filtratioo on Sephadex G-150. The homogeneity of the purified enzyme was verified by polyacrylamide gel electrophoresis. The molecular weight was estimated to be 31,000 on sodium dodecyl sulfate polyacrylamide gel electrophoresis. This enzyme consists of glycine and glutamic acid as major amino acids. The N-terminal and C-terminal residues of the alkaline protease were leucine and histidine respectively.

• The Plant Pathology Journal
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• v.34 no.4
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• pp.286-296
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• 2018

Maintenance of a beneficial microbial community, especially in the rhizosphere, is indispensable for plant growth and agricultural sustainability. In this sense, plant growth-promoting rhizobacteria (PGPR) have been extensively studied for their role in plant growth promotion and disease resistance. However, the impact of introducing PGPR strains into rhizosphere microbial communities is still underexplored. We previously found that the Proteus vulgaris JBLS202 strain (JBLS202) promoted growth of Kimchi cabbage and altered the relative abundance of total bacteria and Pseudomonas spp. in the treated rhizosphere. To extend these findings, we used pyrosequencing to analyze the changes in bacterial communities in the rhizosphere of Kimchi cabbage after introduction of JBLS202. The alterations were also evaluated by taxon-specific realtime PCR (qPCR). The pyrosequencing data revealed an increase in total bacteria abundance, including specific groups such as Proteobacteria, Acidobacteria, and Actinobacteria, in the treated rhizosphere. Time-course qPCR analysis confirmed the increase in the abundance of Acidobacteria, Actinobacteria, Alphaproteobacteria, and Betaproteobacteria. Furthermore, genes involved in nitrogen cycling were upregulated by JBLS202 treatment indicating changes in ecological function of the rhizosphere soil. Overall, these results indicate that introduction of JBLS202 alters both the composition and function of the rhizosphere bacterial community, which can have direct and indirect effects on plant growth. Therefore, we propose that long-term changes in bacterial composition and community-level function need to be considered for practical use of PGPRs.