• Korean Journal of Microbiology
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• v.43 no.1
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• pp.47-53
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• 2007

The DY1 strain of Gram-positive, rod-shaped bacteria was isolated from the soil sample collected from Daeam mountain, Korea. The culture filtrate of DY1 strain showed a broad spectrum of antimicrobial activity on various pathogenic and food poisoning enteric bacterial species tested in vitro. It showed significant growth-inhibitory effect on Salmonella enterica sp., Shigella sp., pathogenic Escherichia coli, Vibrio cholerae, Vibrio parahemolyticus, and Yersinia enterocolitica. For the identification of the DY1 strain, morphological, biochemical and molecular phylogenetic approaches were performed. The DY1 strain was found to be a member of the genus Paenibacillus on the basis of morphological and biochemical analyses. The 16S rDNA of DY1 showed the highest pairwise identity with Paenibacillus polymyxa with 99.79% (1,413 bp/1,416 bp). The antimicrobial entity from DY1 looked different from preciously reported ones and seems to have a great potential to be further studied as a candidate of new antibiotics to control multi-drug resistant pathogens.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.107-116
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• 2006

Korean entomopathogenic nematode strain were evaluated against white grubs, Popillia quadriguttata, Ectinohoplia rufipes and Phyllopertha diversa in laboratory. In addition we examined the efficacy of entomopatogenic nematode, Heterorhabditis sp. KCTC 0991BP strain against white grubs in Seaside Golf Club in Gimpo, Gyeonggii and persistence of entomopatogenic nematode in fairway of Anyang Benest Golf Club in Gunpo, Gyeonggii. In laboratory Heterorhabditis bacteriophora Hamyang strain showed 95% mortality against Popillia quadriguttata. H. bacteriophora Hamyang strain and Heterorhabditis sp. KCTC 0991BP induced 80% mortality against 3rd instar of Ectinohoplia rufipes. However, the letter showed only 60% mortality against Phyllopertha diversa. White grub density was reduced to 39% by the application of Heterorhabditis sp. KCTC 0991BP at the rate of $2.24{\times}10^9$ infective juveniles $ha^{-1}$ compared with control in Seaside Golf Club. Heterorhabditis sp. KCTC 0991BP persisted for two months at $5{\sim}10$ cm soil depth but not persisted at $0{\sim}5$ cm soil depth for the same periods when applied at the rate of $2.47{\times}10^9$ infective juveniles $ha^{-1}$.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.5
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• pp.362-368
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• 2008

In order to isolate novel oligotrophic bacteria exhibiting antifungal activities, soils were collected from pepper-cultivated fields of Yeongyang, Jecheon, Nonsan, Eumsong and Goesan area in Korea. From soils in pepper cultivated area, a total of 9,354 strains were isolated as oligotrophic bacteria by the R2A dilution method. Among 9,354 oligotrohic bacteria candidates, 1A strain was selected by screening against Phytophthora capsici causing phytophthora blight of hot pepper in the greenhouse and field. The strain was identified as Bacillus vallismortis based on its 16S rDNA sequence and key characteristics as compared with those of authentic cultures of B. vallismortis(KACC 12149) and B. mojavensis(KACC 12096). The strain showed broad spectrum of antibiotic activity in vitro test, as revealed in its strong inhibitory activity to the genera Phytophthora, Collectotrichum, Botrytis and Fusarium, but not to Rhizoctonia and Magnaporthe. In pot experiments, infection rate of hot pepper in the non-treated pots was about 89%, while it was only 29% in the pots treated with 1A strain. The result indicated B. vallismortis 1A is a potential biocontrol agent for phytophthora blight of hot pepper

• Microbiology and Biotechnology Letters
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• v.41 no.3
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• pp.263-271
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• 2013

A xylanase-producing bacterium was isolated from a soil sample collected in Yongin city, Korea. The strain was aerobic and gram positive, and grew between pH 5.0 and 11.0, forming a yellow-colored colony. The strain was classified as a novel subspecies bacterium of Paenibacillus barcinonensis by 16S rRNA gene sequence similarity, phylogenetic analysis, phenotypic, and biochemical characteristics, and thus named Paenibacillus sp. HX-1. This strain produced extracellular endo-${\beta}$-1,4-xylanase, and the best xylanolytic activity (205.17 unit/ml) was obtained at 96 h in an optimized TNX medium containing 1% (w/v) bacto tryptone, 1% (w/v) NaCl, and 0.7% (w/v) beechwood xylan at pH 7.0, $37^{\circ}C$ and 200 rpm. The endo-${\beta}$-1,4-xylanase produced by the strain HX-1 yielded xylobiose as the end product from beechwood xylan hydrolysis. The enzyme exhibited optimum pH and temperature at pH 7.0 and $45^{\circ}C$, respectively. The remarkable enhancing effect of the TNX medium on xylanase production by HX-1, in spite of its simple formula, may have great advantages for industrial applications of xylanase.

• Journal of the Korean GEO-environmental Society
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• v.11 no.6
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• pp.5-20
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• 2010

One-dimensional equivalent linear site response analysis is widely used in practice due to its simplicity, requiring only few input parameters, and low computational cost. The main limitation of the procedure is that it is essentially a linear method, in which the time dependent change in the soil properties cannot be modeled and constant values of shear modulus and damping is used throughout the duration of the analysis. Various forms of modified equivalent linear analyses have been developed to enhance the accuracy of the equivalent linear method by incorporating the dependence of the shear strain with the loading frequency. The methods are identical in that it uses the shear strain Fourier spectrum as the backbone of the analysis, but differ in the method in which the strain Fourier spectrum is smoothed. This study used two domestically measured soil profiles to perform a series of nonlinear, equivalent linear, and modified equivalent linear site response analyses to verify the accuracy of two modified procedures. The results of the analyses indicate that the modified equivalent linear analysis can highly overestimate the amplification of the high frequency components of the ground motion. The degree of overestimation is dependent on the characteristics of the input ground motion. Use of a motion rich in high frequency contents can result in unrealistic response.

• Journal of the Korean Geotechnical Society
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• v.27 no.5
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• pp.85-92
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• 2011

The dynamic behavior of piles becomes very complex due to soil-pile dynamic interaction, soil non-linearity, resonance phenomena of soil-pile system and so on. Therefore, the proper numerical simulation of the pile behavior needs much effort and calculation time. In this research, a new modeling method, which can be applied to the conventional finite difference analysis program FLAC 3D, was developed to reduce the calculation time. The soil domain in this method is divided into a near-field region and a far-field region, which is not influenced by the soil-pile dynamic interaction. Then, the ground motion of the far-field is applied to the boundaries of the near-field instead of modeling the far-field region as finite meshes. In addition, the soil non-linearity behavior is modeled by using the hysteretic damping model, which determines the soil tangent modulus as a function of shear strain and the interface element was applied to simulate the separation and slip between the soil and pile. The proposed method reduced the calculation time by as much as one third compared with a usual modeling method and maintained the accuracy of the calculated results. The calculated results by the proposed method showed a good agreement with the prototype pile behavior, which was obtained by applying a similitude law to the 1-g shaking table test results.

• Journal of the Korean Geotechnical Society
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• v.26 no.2
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• pp.23-32
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• 2010

This study was especially conducted to find out the characteristics of the lightweight air-mixed soil (slurry density 10 kN/$m^3$) containing silt related to water. Compression strength, permeability, and capillary height of the lightweight air-mixed soil were studied, and also to support these studies, the structure of that soil was analyzed in detail. Air bubbles of various sizes are inside the lightweight air-mixed soil, and its distribution in a location is almost constant. A numerous tiny pores are inside the air bubbles so that the lightweight air-mixed soil can be saturated with water. Porosity is also estimated through the image analysis. Peak strength of the lightweight air-mixed soil is not dependent on water, but behavior of stress-strain is affected by the water. Permeability is about $4.857{\times}10^{-6}cm/sec$, which is a little bit higher than the clay's permeability. Capillary rise occurs rapidly at the beginning of the test until the lapse of 100 minutes and then its increase rate becomes slow. The capillary rise causes the increase of the density of the lightweight air-mixed soil, and thus it is required to pay attention to this phenomenon during structure design and maintenance of the lightweight air-mixed soil.

• Applied Biological Chemistry
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• v.13 no.1
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• pp.43-50
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• 1970

To study the productivity of single cell protein from the petroleum hydrocarbon utilizing yeasts, 242 soil samples, such as oil soaked soil of gas stations and garage, coal, farm soil, and sewage, from 135 places in Korea were collected. From these samples 468 yeast strains which utilize petroleum hydrocarbon as a sole organic carbon source were isolated and identified by observing the growth rates. For the identified strains optimum culture conditions were determined and analysis of cell components were performed. 1. 90.8% of petroleum hydrocarbon utilizing yeast strains were found from oil soaked soil and about 10% from coal, farm soil and sewage etc. 2. The yeast strain of the highest cell productivity was isolated from oil soaked soil and was identified as Candida curvata HY-69-19. 3. The optimum culture conditions for the selected yeast strain were found to be pH 5.0, $28^{\circ}C$ and affluent aerated state. 4. Candida curvata HY-69-19 was found to utilize favorably the heavy gas oil fractionated at above $268.9^{\circ}C$ as carbon source and urea as inorganic nitrogen source. 5. The growth curve of this strain on heavy gas oil medium showed that the yeast has a lag phase up to 18 hours and logarithmic growth phase between 24 to 42 hours. Generation time was found to be between 3.8 and 4.5 hours during the logarithmic growth phase. 6. About 300 mg dried cells per heavy gas oil was harvested under the culture conditions of adjusted pH to 5.0 at time intervals of 6 hours for 54 hours and heavy gas oil urea for shaking culture medium. 7. Chemical composition of the yeast cell was found to be 40.25%, 14.81%, 24.32% and 10.63% for crude protein, crude lipid, carbohydrate and ashes, respectively.

• Journal of the Korean Geotechnical Society
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• v.30 no.2
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• pp.5-18
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• 2014

Depending on the underground load support mechanism, anchors are classified as friction anchors, bearing plate anchors and the recently developed combined friction-bearing plate anchors which combine the characteristics of both the friction and bearing plate type anchors. Even though numerous studies have been performed on bearing plate anchors, there were only few studies performed to observe the failure surface of bearing plate anchors. Furthermore most of the soil materials used on these tests were not real sand but carbon rods. In this study, sand was placed in the soil tank and laboratory tests were performed with bearing plate anchors installed with an embedment depth (H/h) ranging from 1~6. The variation in the pullout capacity and the behaviour of soil with the embedment depth (H/h) were observed. Ground deformation analysis program was also used to analyze soil displacement, zero extension direction, maximum shear strain contours. It was determined from the analysis of the results that at ultimate pullout resistance the deformation was 5 mm and the failure surface occurred in a narrower area when compared with results of the previous researches. It was also observed that the width of the fracture surface gradually becomes wider and expands up to the surface as the deformation increases from 10 mm to 15 mm.

• Journal of Applied Biological Chemistry
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• v.54 no.2
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• pp.79-83
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• 2011

Over-application of nitrogen fertilizer keeps increasing the salinity in the soils of greenhouse in domestic agriculture. In order to remove the excess amounts of soil nitrate, soil microorganisms which have high capacity of nitrate uptake were isolated from the upland soils and their nitrate uptake activities were measured. Strain GS2 was able to remove 50 mM nitrate within 12 h. After sequence comparison analysis of 16S rRNA gene, the strain was identified and named as Bacillus sp. GS2. When the growth and nitrate uptake activities were measured, maximal values were obtained at $30-40^{\circ}C$ and $37^{\circ}C$, respectively; however, both were optimal at pH 6-8. In the media containing 50 mM nitrate, Bacillus sp. GS2 removed 43 mM nitrate which is corresponding to 86% removal. Similar amounts of nitrate removal were observed at the nitrate concentrations up to 300 mM, showing a saturation in nitrate uptake at concentrations above 50 mM. These results imply that Bacillus sp. GS2 can be a good candidate for the microbial remediation of accumulated environmental nitrate because of its excellent growth and nitrate uptake activity.