• Microbiology and Biotechnology Letters
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• v.27 no.5
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• pp.391-398
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• 1999

A methylotrophic actinomycetes strain MO-16, which produce the antimicrobial compound, was isolated from soil and supposed as Amycolatopsis sp. based on taxonomic studies. The cell-free extract of methanol-grown strain MO-16 showed dehydrogenase activity for methanol and formaldehyde when various electron acceptors were added for oxidation. On the other hand, methanol did not affect the production of antimicrobial compounds, and organic nitrogen sources such as corn steep liquor and peptone were better than inorganic nitrogen sources. These compounds showed broad antimicrobial spectrum to the tested strains such as bacteria and yeast. The antimicrobial comounds were very stable under heat(121$^{\circ}C$), acid(pH2.0), alkali(pH11.0) treatments. These compounds were isolated by ethylacetate extract, silica gel column chromatography and reverse phase HPLC. Two compounds(peak 1 and 2) were detected as antimicrobial compounds through the HPLC analysis. The peak 2 was purified as a single compound and revealed a 98% purity.

• Microbiology and Biotechnology Letters
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• v.27 no.4
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• pp.286-291
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• 1999

By using autoradiography and phosphate medium, high phosphate accumulating bacteria were isolated from the soil of protected cultivation area and activated sludge. Selected strain, PO8, was gram-negative, rod/spherial(0.5~0.6$\times$1.0~1.2${\mu}{\textrm}{m}$ in size) and non-motile. PI4, another selected strain, was gram-negative, rod(0.4~0.5$\times$1.5~1.6${\mu}{\textrm}{m}$ in size) and motile. It also had flagella. According to their morphological, physiological and biochemical properties, the stains were identified as Acinetobacter lwoffi PO8 and Chromobacterium lividum PI4, respectively. A. lowoffi PO8 and C. lividum PI4 cultured in the P-1 medium containing 150ppm phosphate were able to uptake high phosphate up to 92% and 85%, respectively after 24 hours at 3$0^{\circ}C$ during liquid culture.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.311-317
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• 2008

This study was carried out to improve our understanding about the influence of silt content on the stress-strain of sand under cyclic loading. Soil specimens were prepared by wet-tamping method as same void ratio and specimen's silt contents on total weights was changed from 0% to 20%. Also, effects of the silt contents on the stress-strain response were studied at different anisotropic consolidation ratio, Kc=1.0, 1.5, 2.0 condition. As a result, cyclic shear strength decreased as silt contents increased in same stress ratios. In same silt contents, cyclic shear strength increased as Kc increased in lower silt contents, but in higher silt contents, it had reverse results.

• Journal of Environmental Health Sciences
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• v.27 no.4
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• pp.35-40
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• 2001

In order to evaluate the safety of agricultural products in Korea, we carried out work by screening of Fusarium species. which can produce deoxinivalenol(DON) from agricultural products in Western Gyeongnam, Korea. From 215 samples of soil agricultural products, 129 strains of Fusarium species were obtained. The production of DON was verified by thin layer chromatography(TLC) As the results of TLC, 25 strains were identified as DON producing strain. But only 10 strains were identified as DON producing strains by enzyme-linked immunosorbent assay (ELISA). The maximum DON producing strain No.41 was isolated from corn. In conclusion. the above results indicate that DON producing fungi contaminated agricultural products in Korea. Therefore further studies are required to accumulate more detailed data about the contamination of DON in various agricultural products.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.3
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• pp.441-447
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• 1998

A bacterial strain No. 43 was isolated from soil samples as a levan-assimiating microorganism producing an extracellular levanase and hydrolying levan to levanbiose. According to the taxonomic characteristics of its morphological and physiological properties, the strain was idenified as Pseudomonas sp. No. 43. The optimum culura medium was composed of 10g levan, 5g(NH4)2SO4, 3g NH4Cl, 3g polypepton, 1g K2HPO4, 0.5gMgSO4.7H2O, and 0.2g MnCl2.4H2O per liter. The cultivation for levanase was carried out in pH 7.0 at 4$0^{\circ}C$ for 28hr. The reaction product was a kind of oligosaccharide and it was purified by chilled ethanol precipitation and gel filtration for evalluation of degree of polymerization (DP). The purified product was determined as levanbiose of MW 342 and DP2 by HPLC and FAB-MS.

• The Korean Journal of Food And Nutrition
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• v.11 no.6
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• pp.622-628
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• 1998

Microorganisms isolated from soil were tested for their flocculating activity in kaolin suspension, Identification of the best producing CH-23 strain showed that the strain belonged to the Bacillus megaterium. The maximum production of the flocculating from Bacillus megaterium CH-23 was observed in the culture medium containing 2% sucrose, 3% NaNo3, 0.1% K2HPO4, 0.5% NaCl, 0.5% MgSO4.7H2O and 0.01% tryptone at initial pH 7.0 and 25~3$0^{\circ}C$. Flocculating activity was improved to 57% when the culture medium contained Mn2+(0.01% MnSo4). In the culture medium containing Mg2+(0.01% MgSO4.7H2O) and Ca2+(0.01% CaCO3), flocculating activity were reached to 48% and 33%, respectively.

• Wind and Structures
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• v.4 no.2
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• pp.163-176
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• 2001

Two 12 m high tubular steel lighting columns have been instrumented to determine the wind-induced fatigue loading experienced by such columns. Each column supported a single luminaire mounted on a 0.5 m long bracket. One column was planted in soil, and the other bolted through a welded baseplate to a substantial concrete base. The columns were strain gauged just above the shoulder weld which connected the main shaft to the larger base tube. Forced vibration tests were undertaken to determine the natural frequencies and damping of the columns. Extensive recordings were made of response to winds with speeds from 4 m/s to 17 m/s. Selected records were analysed to obtain stress cycle counts and fatigue lives. Mean drag coefficients were also derived from the strain data to investigate experimentally the effect of Reynolds Number.

• Archives of Pharmacal Research
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• v.12 no.3
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• pp.176-180
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• 1989

Four bacterial strains having inducible resistance to erythromycin were isolated from soil samples in Korea and characterized. MLS inducibility was checked in each strain. Cloning of inducible resistance gene(s) has been tried. Four isolates were identified as B. anthracis, Micrococcus luteus, Streptococcus faecium and B. licheniformis, in which erythromycin, oleandomycin, cirramycin and carbomycin acted as resistance inducers respectively. The resistance gene cloned from B, licheniformis 597 strain using pBS 42 vector was found to have a 3.2 kb insert.

• Proceedings of the Korean Geotechical Society Conference
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• 2001.03a
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• pp.215-222
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• 2001

Uncertainty study of shear strength characteristics of the marine clays was carried out based ell In-situ tests and laboratory tests on tile south-east coastal region of the Korean peninsula. Theoretical analyses were studied using both tile spherical cavity expansion theory in finite soil mass and the strain path method to determine tile cone factor using the undrained shear strengths obtained by in-situ tests, and the empirical methods in accordance with the ultimate resistance theory were also discussed. Analysis show that the empirical methods suggest more reasonable value than that of theoretical methods in terms of comparing the cone factor estimated using linear regression and frequency distribution analyses. The cone factors obtained by the empirical methods are 18, 15, and 6 respectively, from the results of total cone resistance, effective cone resistance, and excess porewater cone resistance method, and the estimated were similar to those of previous researcher's.

• Proceedings of the Korean Geotechical Society Conference
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• 2001.03a
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• pp.485-492
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• 2001

Weathered soil is one of the most representative soils in Korea. In this study, a series of cyclic triaxial tests was carried out to predict the post-cyclic deformation behavior of weathered soils in case of non-liquefaction. Excess pore pressure response during cyclic loading and volumetric strain during the dissipation of excess pore pressure were measured varying the confining pressure, relative density and cyclic stress ratio. Based on the test results, it Is found that the modified excess pore pressure ratio, excess pore pressure ratio normalized by cyclic stress ratio, is uniquely correlated with the number of cycles irrespective of confining pressure and cyclic stress ratio. Using the newly proposed MEPPR(modified excess pore pressure ratio) concept, it is possible to easily evaluate the excess pore pressure and the settlement of weathered soils due to cyclic loading by greatly reduced number of tests. It is also verified that the reconsolidation volumetric strain is independent of the way how the excess pore pressure was generated.