Archives of Pharmacal Research
- Volume 12 Issue 3
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- Pages.176-180
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- 1989
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
The Cloning of MLS Antibiotics Inducible Resistance Gene
- Choi, Eung-Chil (College of Pharmacy, Seoul National University) ;
- Woo, Kyung-Won (College of Pharmacy, Seoul National University) ;
- Kwak, Jong-Suk (College of Pharmacy, Seoul National University) ;
- Kim, Jin-Hwan (College of Pharmacy, Seoul National University) ;
- Byung-Kak (College of Pharmacy, Seoul National University)
- Published : 1989.09.01
Abstract
Four bacterial strains having inducible resistance to erythromycin were isolated from soil samples in Korea and characterized. MLS inducibility was checked in each strain. Cloning of inducible resistance gene(s) has been tried. Four isolates were identified as B. anthracis, Micrococcus luteus, Streptococcus faecium and B. licheniformis, in which erythromycin, oleandomycin, cirramycin and carbomycin acted as resistance inducers respectively. The resistance gene cloned from B, licheniformis 597 strain using pBS 42 vector was found to have a 3.2 kb insert.