• Microbiology and Biotechnology Letters
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• v.37 no.4
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• pp.316-321
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• 2009

We have isolated Chondromyces crocatus KYC2823 in pure culture and five other strains in mixed culture with companion bacteria from Korean soil samples. The strain KYC2823, which was isolated from the soil sample collected in Cheongdo-gun, Gyeongsangbuk-do, showed typical characteristics of C. crocatus, including the shape of fruiting bodies and production of a peculiar odor. In addition, the 16S rDNA sequence was 99.8% identical to that of the strain Cm c5, the proposed neotype strain of C. crocatus. Cloning and sequence analysis of the polyketide biosynthetic genes from KYC2823 by performing PCR have revealed that this strain has biosynthetic gene clusters for ajudazols (inhibitors of electron transport systems) and chondramides (substances affecting the function of the actin cytoskeleton), and biosynthetic genes for other polyketide compounds that have not been cloned yet.

• Journal of the Korean Society of Hazard Mitigation
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• v.4 no.2 s.13
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• pp.19-27
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• 2004

The interest in the dynamic properties of soils has increased strongly because of earthquake, heavy traffic, and foundations undergo high amplitude of vibrations. Most of soils in Korean peninsula are composed of granite soils, especially the decomposed mudstone soils are widely spread in Pohang areas, Kyong-buk province. Therefore, it is very important to investigate the dynamic properties of these types of soils. The most important soil parameters under dynamic loadings are shear modulus and material dampings. Furthermore, few definitive data exist that can evaluate the behavior of unsaturated decomposed mudstone soils under dynamic loading conditions. The investigations described in this paper is designed to identify the shear modulus and damping ratio due to a surface tension for the unsaturated decomposed mudstone soils under low and high strain amplitude. For this purpose, the resonant column test and the cyclic triaxial test were performed. Test results and data have shown that the optimum saturated degree of decomposed mudstone soils under low and strain amplitude is $32{\sim}37%$ which is higher than that of decomposed granite due to the amount of fine particles as well as the type and proportion of chief rock-forming minerals.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.4
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• pp.318-326
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• 2016

Two phosphate solubilizing bacteria, Pantoea rodasii PSB-11and Enterobacter aerogenes PSB-12, were isolated from button mushroom compost and employed to assess their synergistic effect in liquid medium and on growth of green gram plants by single and co-inoculation of the strains. Co-inoculation of two strains was found to release the highest content of soluble phosphorus ($521{\mu}g\;ml^{-1}$) into the medium, followed by single inoculation of Pantoea strain ($485{\mu}g\;ml^{-1}$) and Enterobacter strain ($470{\mu}g\;ml^{-1}$). However, there was no significant difference between single inoculation of bacterial strain and co-inoculation of two bacterial strains in terms of phosphorous release. The highest pH reduction, organic acid production and glucose consumption was observed in the E. aerogenes PSB-12 single inoculated culture medium rather than those of co-inoculation. According to the plant growth promotion bioassay, co-inoculated mung bean seedlings recorded 10.6% and 10.7% higher shoot and root growth respectively compared to the control. Therefore, in concluding, co-inoculation of the strains P. rodasii and E. aerogenes displayed better performance in stimulating plant growth than inoculation of each strain alone. However, being short assessment period of the present study, we recommend in engaging further works under field conditions in order to test the suitability of the strains to be used as bio-inoculants.

• Journal of the Korean Geotechnical Society
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• v.20 no.3
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• pp.151-159
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• 2004

This paper describes a systematic way of identifying the horizontal coefficient of consolidation of clayey soil by applying an optimization technique to the early part of dissipation data measured from the self-boring pressuremeter strain holding test. An analytical solution developed by Randolph ＆ Wroth (1979) was implemented in normalized form to express the build-up of excess pore pressures as a function of the rigidity index and subsequent dissipation of excess pore pressures around a pressuremeter Horizontal coefficient of consolidation was determined by minimizing the differences between theoretical and measured excess pore pressure curves over 50% degree of dissipation range using optimization technique. The effectiveness of the proposed back-analysis method was examined against the real fled performances obtained from pressuremeter strain holding tests at Gimje and Yangsan site. It is shown that the proposed back-analysis method can evaluates the rational horizontal coefficient of consolidation, which is similar to those obtained from the piezocone dissipation test. Furthermore, proposed method can evaluate appropriate coefficient of consolidation for soil under partially drained condition.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.705-711
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• 2007

Two novel strains of the Cytophaga-Flexibacter-Bacteroides(CFB) group, designated Gsoil $219^T$ and Gsoil $238^T$, were isolated from soil of a ginseng field of Pocheon Province in Korea. Both strains were Gram-negative, aerobic, nonmotile, nonspore-forming, and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that both isolates belong to the genus Chitinophaga but were clearly separated from established species of this genus. The sequence similarities between strain Gsoil $219^T$ and type strains of the established species and between strain Gsoil $238^T$ and type strains of the established species ranged from 91.4 to 94.7% and 91.6 to 94.2%, respectively. Phenotypic and chemotaxonomic data(major menaquinone, MK-7; major fatty acids, $iso-C_{15:0}\;and\;C_{16:1}\omega5c$; major hydroxy fatty acid, $iso-C_{17:0}3-OH$; major polyamine, homospermidine) supported the affiliation of both strains Gsoil $219^T$ and Gsoil $238^T$ to the genus Chitinophaga. Furthermore, the results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of both strains from the other validated Chitinophaga species. Therefore, the two isolates represent two novel species, for which the name Chitinophaga soli sp. nov.(type strain, Gsoil $219^T=KCTC\;12650^T=DSM\;18093^T$) and Chitinophaga terrae sp. nov.(type strain, Gsoil $238^T=KCTC\;12651^T=DSM\;18078^T$) are proposed.

• Journal of the Korean Geotechnical Society
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• v.34 no.2
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• pp.5-17
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• 2018

In this study, the in-situ stress, strength and stress-strain characteristics with shear parameters (UU, CU, ${\bar{CU}}$) are analytically evaluated and the stability analyses are carried out under loading/unloading conditions. The in-situ stress and the stress-strain behaviour may become different according to input shear parameters in finite element analyses with construction step, Especially, if the internal friction angle in Mohr-Coulomb model is set to zero, the in-situ stress and the stress-strain behaviour might not be properly predicted. The results from CU parameter of total stress analysis have no significant difference with the results from CU of effective stress analysis. Therefore, in the numerical analysis for soft ground, CU parameters can be applied to predict in-situ stress and stress-strain behaviors. In addition, the calculation method was proposed to determine the shear parameter of Mohr-Coulomb model, which is corresponding to the shear strength equivalent to that of in-situ soil.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.836-840
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• 2011

In order to isolate thermophilic compost-promoting bacteria with high activity of cellulase and xylanase, spent mushroom substrates with sawdust were collected from mushroom cultivation farm, Jinju, Gyeongnam in Korea. Among of the isolates, one strain, designated SJ21 was selected by agar diffusion method. The strain SJ21 was identified as members of the Bacillus lincheniformis by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain SJ21 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rDNA gene sequence similarity of 99%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain SJ21 was classified within the genus Bacillus, for which the name Bacillus sp. SJ21 is proposed. The cellulase and xylanase activity of Bacillus sp. SJ21 was slightly increased according to bacterial population from exponential phase to stationary phase in growth curve for Bacillus sp. SJ21.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.3
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• pp.185-191
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• 2001

To obtain the basic information for degradation of remaining pesticide accumulated in cultivated soil of Honam area, the resistant bacterial strains were investigated in Chlorothalonil(TPN). Mancozeb, Bentazone, and Butachlor levels of 100, $500{\mu}g\;ml^{-1}$, and degradation of TPN by TPN-resistant bacteria in sterilized soil was studied under TPN levels 0, 10, 50 and $100{\mu}g\;g^{-1}$. A number of resistance strains were decreased with higher at concentration level of pesticide, and were higher in greenhouse than upland or paddy soil. The resistance of bacteria was strong in other of Bentazone> Butachlor> TPN> Mancozeb. The percentage of bacterial strains of resistance for pesticides isolated from the cultivated soil were the highest in Acinetobacter spp. and Corynebacterium spp., and the lowest in Moraxella spp. A number of TPN-resistant strains were the highest at the TPN level of $10{\mu}g\;g^{-1}$, and 5 days after strains inoculation, and were higher in Pseudomonas spp. TD-25 than TC-23 or strains in non-sterilized soil. The degradation of TPN was fast in order of strain TD-25>strain in non-sterilized soil >TC-23.

• Journal of the Korean Geotechnical Society
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• v.26 no.11
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• pp.17-28
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• 2010

Vertical drains are commonly used to accelerate the consolidation process of soft soils, such as dredged materials, because they additionally provide a radial drainage path in a deep soil deposit. In practice, vertical drains are commonly installed in the process of self-weight consolidation of a dredged soil deposit. The absence of an appropriate analysis tool for this situation makes it substantially difficult to estimate self-weight consolidation behavior considering both vertical and radial drainage. In this paper, a new method has been proposed to take into account both vertical and radial drainage conditions during nonlinear finite strain self-weight consolidation of dredged soil deposits. For 1-D nonlinear finite strain consolidation in the vertical direction, the Morris (2002) theory and the PSDDF analysis are adopted, respectively. On the other hand, to consider the radial drainage, Barron's vertical drain theory (1948) is used. The overall average degree of self-weight consolidation of the dredged soil is estimated using the Carillo formula (1942), in which both vertical and radial drainage are assembled together. A series of large-scale self-weight consolidation experiments being equipped with a vertical drain have been carried out to verify the analysis method proposed in this paper. The results of the new analysis method were generally in agreement with those of the experiments.

• Geomechanics and Engineering
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• v.23 no.2
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• pp.179-187
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• 2020

Freezing-thawing cycles have significant effect on soils engineering behavior in frozen areas. This effect is more considerable in fine-graded than coarse-grained soils. The objective of this study is improving soil durability and strength in continues freezing-thawing cycles. For getting this purpose mixture of Polyvinyl Acetate (PVAc) and Ethylene Glycol Monobutyl Ether (EGBE) has been added to fine-grained soil and final prepared samples were tested at different freezing-thawing cycles. PVAc was mixed with 1%, 2% and 3% of soil weight. Half of PVAc weight was used as weight of EGBE. Freezing-Thawing cycles were exposed to samples and they were tested at different cycles. Results showed that adding mixture of PVAc+EGBE improved strength and durability of samples up to 10 freezing-thawing cycles. Unconfined compress strength tests were applied to samples and stress and strain of samples were tested on failure time. Behavior of samples was different at different percentages of mixture. Results showed that increasing amount of PVAc from 1% to 2% had more considerable effect on final stress than 2% to 3%. Using higher percentages of PVAc + EGBE mixture leaded to that samples carried more strain before collapsing. Another result gained from tests was that, freezing-thawing effect was more considerable after fourth cycles. It means differences between first and fourth cycles were more considerable than differences between fourth and tenth.