• Journal of Microbiology
• /
• v.44 no.4
• /
• pp.432-438
• /
• 2006

Twelve actinomycete strains were isolated from Egyptian soil. The isolated actinomycete strains were then screened with regard to their potential to generate antibiotics. The most potent of the producer strains was selected and identified. The cultural and physiological characteristics of the strain identified. the strain as a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1.5kb) of the most potent strain evidenced a 99% similarity with Streptomyces spp. and S. aureofaciens 16S rRNA genes, and the isolated strain was ultimately identified as Streptomyces sp. MAR01. The extraction of the fermentation broth of this strain resulted in the isolation of one major compound, which was active in vitro against gram-positive, gram-negative representatives and Candida albicans. The chemical structure of this bioactive compound was elucidated based on the spectroscopic data obtained from the application of MS, IR, UV, $^1H$ NMR, $^{13}C$ NMR, and elemental analysis techniques. Via comparison to the reference data in the relevant literature and in the database search, this antibiotic, which had a molecular formula of $C_{19}H_{29}NO_2$ and a molecular weight of 303.44, was determined to differ from those produced by this genus as well as the available known antibiotics. Therefore, this antibiotic was designated Meroparamycin.

• Journal of Microbiology and Biotechnology
• /
• v.2 no.2
• /
• pp.78-84
• /
• 1992

Streptomyces sp. No.8, which produced glucose isomerase was isolated from soil samples. The isolated strain, No.8, was identified as belonging to the Genus Streptomyces. A mutant strain, SM 805, showed the greatest ability to produce glucose isomerase. It was developed from the strain, No.8, by mutagenesis induced by NTG and UV treatment. The mutant strain, SM 805, produced about 7 times more glucose isomerase than the parental strain, No.8. This enzyme catalyzed the isomerization of D-xylose, D-glucose and D-ribose. It was inactive in the absence of metal ions, but was activated by the addition of $Mg^{2+}$ or $Co^{2+}$. The optimum temperature and pH for enzyme activity were $80^\circ{C}$ and pH 8.5, respectively. The enzyme was stable in a pH range of 6.0 to 10.0, and it was highly thermostable. There was no activity loss below $80^\circ{C}$, and even above $90^\circ{C}$ about 45% of its activity was retained. The reaction equilibrium was reached when about 53% fructose was present in the reaction mixture. Whole cells containing glucose isomerase from Streptomyces sp. SM 805 were immobilized by glutaraldehyde treatment. The resultant immobilized enzyme pellets showed a relatively long stability during the isomerizing reaction. The half-life of the immobilized enzyme during the operating was 45 days in the presence of 10mM $Mg^{2+}$.

• Journal of Species Research
• /
• v.9 no.3
• /
• pp.204-209
• /
• 2020

In 2019, as a subset study to discover indigenous yeast species in Korea, a total of 20 yeast species were isolated from soil samples collected in Pochoen-si. Among them, eight strains were unreported species. From the high 26S rRNA gene sequence similarity and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to independent and predefined yeast species. The 20 strains were assigned to the genera Aureobasidium (1 strain) and Meyerozyma (1 strain) of the phylum Ascomycota and Cystofilobasidium (2 strains), Filobasidium (1 strain), Naganishia (2 strains), Bullera (3 strains), Leucosporidium (9 strains) and Sampaiozyma (1 strain) of the phylum Basidiomycota. There is no official report of the following species in Korea: Leucosporidium creatinivorum (4 strains), Leucosporidium escuderoi(2 strains), Leucosporidium golubevii(1 strain) and Leucosporidium intermedium (2 strains). Basic biochemical characteristics, colony and cell morphology are also described in the species description section.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.38 no.3
• /
• pp.70-81
• /
• 1996

Solutions of geotechnical engineering problems require predictions of deformation and stresses during various stages of loading. Powerful numerical methods are available to make such predictions even for complicated problems. To get accurate results, realistic stress-strain relationships of soils are dependent on a number of factors such as soil type, density, stress level and stress path. Attempts are continuously being made to develope analytical models for soils incorporating all such factors. Isotropic compression-expansion test and a series of drained conventional triaxial tests with several stress path for Baekma river sand were performed to investigate stress-strain and volume change characteristics of Lade's single work hardening model dependant on the stress path. In order to predicted of stress-strain and volumetric strain behavior were determined the values of parameters for the mode by the computer program based on the regression analysis. Predicted stress-strain behavior of triaxial compression tests and optional stress path tests for increasing confining pressure with parameters obtained conventional triaxial compression tests agreed with several test results but the prediction results for decreasing confining pressure reduced triaxial compression tests make a little difference with test results.

• YAKHAK HOEJI
• /
• v.37 no.4
• /
• pp.389-396
• /
• 1993

An Actinomycetes strain JB isolated from Mt. Hanla had a strong antimicrobial activity against gram positive bacteria and tumor cell growth inhibition. Especially, it couldn't degrade starch and casein as organic compounds. It was resist on lincomycin and rifampicin. The spore mass of strain JB which was arethrospore was white. DAP of the cell wall was L, L-DAP. Antimicrobial material was heat stable, dissolved in ethyl acetate, and not dissolved in butanol. In the pressnce of 0.1% phenol and 4% sodium chloride, strain JB could grow, but it didn't growth at below $10^{\circ}C$. Strain JB didn't use dextran, sodium acetate and sodium citrate as sole carbon source and L-cystein and L-thereonine as nitrogen source. The filtered broth of strain JB had the antimicrobial activity against gram positive bacteria, especially Staphylococcus aureus (ATCC 65389) and the growth inhibition of tumor cell line.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.10
• /
• pp.1597-1604
• /
• 2006

To screen for an anti-yeast substance (AYS), many bacteria were isolated from soil and a strain AY2000 was selected. The strain AY2000 was identified as Rahnella aquatilis by morphology, biochemical properties, and 16S r-RNA nucleotide sequence analyses. The strain AY2000 showed anti-yeast activity against Candida albicans and Saccharomyces cerevisiae, whereas R. aquatilis ATCC33071 as a type strain did not show the activity against the yeasts under the same condition. The growth of yeast cell was significantly inhibited by AYS produced by the strain AY2000, as shown by optical density and MTT assay. The minimum inhibitory concentration (MIC) of the AYS against S. cerevisiae and C. albicans at $28^{\circ}C\;was\;20{\mu}g/ml\;and\;60{\mu}g/ml$, respectively. The MIC of AYS against hyphae of C. albicans at $37^{\circ}C\;was\;600{\mu}g/ml$. Scanning electron microscopic analysis revealed that yeast cells treated with AYS had an irregular form with a wrinkled and rough surface.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2002.10a
• /
• pp.585-592
• /
• 2002

Discharge capacity of PBD is the most important factor of specification items to control any product of PBD. There is no standard specification for the PBD. Because the degree of discharge capacity is related to well resistance, install depth, maximum strain etc in the field. Discharge capacity test of PBD, permeability test of filter are conducted using PBD materials used in Korea. This paper proposes the critical discharge capacity for deep PBD under condition of non well resistance based upon their test and theoretical calculation. It was found that discharge capacity more than about 10 ㎤/sec is enough to undergo designing of deep PBD without well resistance.

• The Korean Journal of Mycology
• /
• v.46 no.4
• /
• pp.369-374
• /
• 2018

The fungal strain KNU16-004 was isolated from a field soil sample collected in Seoul. The isolate was identified as Leptosphaerulina australis based on morphological characterization and phylogenetic analysis using the internal transcribed spacer (ITS), large subunit (LSU) rDNA regions, and ${\beta}-tubulin$ (Tub2). This is the first report of Leptosphaerulina australis in Korea.

• Environmental Engineering Research
• /
• v.13 no.1
• /
• pp.8-13
• /
• 2008

The extent of solubility enhancement by biosurfactant was examined at various pHs prior to the biodegradation experiments. The molar solubilization ratio (MSR) was calculated from the batch solubilization experiments and the highest MSR was detected at pH 5. The effect of the biosurfactant, rhamnolipids, on the phenanthrene mineralization in soil-water system was investigated. The strain 3Y was selected for the mineralization assay and large amounts of phenanthrene were degraded at neutral pH in soil-water system without the biosurfactant. The addition of 150 mg/L rhamnolipids showed no effect on mineralization of phenanthrene in soil-water system, and total mineralization rates after 6 weeks incubation at each pH showed no differences in presence and absence of rhamnolipids. Our result indicated that the toxic effect of rhamnolipids can disappear when soil particles exist, and also the enhanced solubility of phenanthrene does not work for mineralization enhancement in this soil-water system.

• Proceedings of the Earthquake Engineering Society of Korea Conference
• /
• 2002.03a
• /
• pp.125-132
• /
• 2002

In this study, a numerical method is developed for nonlinear analysis for soil-pile-structure interaction system in time domain. Finite elements considering material nonlinearity are used for the near field and boundary elements for the far field. In the near field, frame elements are used for modeling a pile and plane-strain elements for surrounding soil and superstructure. In. the far field, boundary element formulation using the dynamic fundamental solution is adopted and coupled with the near field. Transformation of stiffness matrices of boundary elements into time domain is performed by inverse FFT. Stiffness matrices in the near field and far field are coupled. Newmark direct time integration method is applied. Developed soil-pile-structure interaction analysis method is verified with available literature and commercial code. Also, parametric studies by developed numerical method are performed. And seismic response analysis is performed using actual earthquake records.