• Title/Summary/Keyword: soil strain

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Isolation of Methylotrophic Actionmycetes Capable of Producing Antagonistic Activity Against Oral Resident Bacteria and Screening of Mutants (구강상재균을 중심으로 항균력을 나타내는 메탄올 자화방선균의 분리 및 변이주 생산)

  • Park, Myoung-Ho;Lee, Hwa-Sik;Bae, Bong-Jin;Kim, Joung
    • Journal of Technologic Dentistry
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    • v.22 no.1
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    • pp.145-152
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    • 2000
  • In order to select an effective antibiotic substance against oral resident bacteria, we were isolated from soil and texonomically analyzed. Seven hundred and eighteen strains were isolated on humic acid- vitamin agar(HV agar) and 220 strains were on methanol medium from three each paddy forest, field and riverside soil samples. So, during the screening of antibiotics from soil, we isolated microorganisms showing powerful antagonistic activity against oral resident bacteria. Microorganism was tested against 25 strains of bacteria, yeast and fungi. Among them, No. 248 strain exhibited the most strongly growth inhibition. So, the taxonomical analysis the isolated strain was found to be unknown Actinomyces sp. and was named No 248. A production of the antibiotics from No. 248 begins at the early exponential phase developed at the 72th hour under the optinum conditions. The property of No. 248 antimicrobial compound was very stable under acid(pH 3.0) and alkali(pH 10.0) treatment, but it was instable in heat treatment at $120^{\circ}C$. For the improvement of antibiotic activity, two mutants were isolated from strain No. 248 by the treatment of mutagenic agents, NTG and hydroxylamine. As a result, the mutant strains excreted the potent antibiotics to inhibit the growth of Candida albicans.

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Strain-dependent dynamic properties of cemented Busan clay (부산 고결점토의 변형률 의존적 동적거동특성에 관한 연구)

  • Kim, Ah-Ram;Chang, Il-Han;Cho, Gye-Chun;Shim, Sung-Hyun;Kang, Yeoun-Ike
    • Proceedings of the Korean Geotechical Society Conference
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    • 2010.09b
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    • pp.61-67
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    • 2010
  • Thick soft clay deposits which are generally located at the west and south coast of the Korean peninsula have complicated characteristics according to their orientation and formation history. Thus, several geotechnical problems could possibly occur when those soft clay deposits are used as foundations for marine structures. Deep cement mixing (DCM) method is one of the most widely used soft soil improvement method for various marine structures, nowadays. DCM method injects binders such as cement into the soft ground directly and mixes with the in-situ soil to improve the strength and other geotechnical properties sufficiently. However, the natural impacts induced by dynamic motions such as ocean waves, wind, typhoon, and tusnami give significant influences on the stability of marine structures and their underlaying foundations. Thus, the dynamic properties become important design criteria to insure the seismic stability of marine structures. In this study, the dynamic behavior of cemented Busan clay is evaluated. Laboratory unconfined compression test and resonant column test are performed on natural in-situ soil and cement mixed specimens to confirm the strength and strain-dependent dynamic behavior variation induced by cement mixing treatment. Results show that the unconfined compressive strength and shear modulus increase with curing time and cement content increment. Finally, the optimized cement mixing ratio for sufficient dynamic stability is obtained through this study. The results of this study are expected to be widely used to improve the reliability of seismic design for marine structures.

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Engineering Characteristics of Sam Cheok Organic Soil (삼척 유기질토의 공학적 특성)

  • Kim, Sang-Gyu;Choe, In-Geol;Park, Yeong-Mok
    • Geotechnical Engineering
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    • v.12 no.1
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    • pp.21-34
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    • 1996
  • This paper presents the engineering characteristics of the Samcheok organic soil which contains a large portion of organic matter and high natural water content. A series of lab tests shows that the measured liquid limit is quite different depending on preparing methods of specimens. The values tested with natural condition are higher up to 4 times than thole of the oven dry specimen. It is shown that the organic soils fail at large strain and do not show peak stress in the stress strain relationships. Also strength increase ratios, which are measured 0.43 to 0.65 in this tests, are significantly higher than those of the soft clay without organic matter. The consolidation tests indicate that the verti'cal and horizontal Permeabilities are almost the same. For the remolded samples is reduced from 112 to 116 of the vertical permeability An increase of organic matter or water content of the organic soils results in an increase of the coefficient of secondary consolidation. The increase rate is slow below 15 percents of the organic contents while the rate becomes higher above the value.

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Experimental Study on Unconfined Compression Strength and Split Tensile Strength Properties in relation to Freezing Temperature and Loading Rate of Frozen Soil (동결 온도와 재하속도에 따른 동결토의 일축압축 및 쪼갬인장 강도특성)

  • Seo, Young-Kyo;Choi, Heon-Woo
    • Journal of Ocean Engineering and Technology
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    • v.26 no.6
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    • pp.19-26
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    • 2012
  • Recently the world has been suffering from difficulties related to the demand and supply of energy due to the democratic movements sweeping across the Middle East. Consequently, many have turned their attention to never-developed extreme regions such as the polar lands or deep sea, which contain many underground resources. This research investigated the strength and initial elastic modulus values of eternally frozen ground through a uniaxial compression test and indirect tensile test using frozen artificial soil specimens. To ensure accurate test results, a sandymud mixture of standard Jumunjin sand and kaolinite (20% in weight) was used for the specimens in these laboratory tests. Specimen were prepared by varying the water content ratio (7%, 15%, and 20%). Then, the variation in the strength value, depending on the water content, was observed. This research also established three kinds of environments under freezing temperatures of $-5^{\circ}C$, $-10^{\circ}C$, and $-15^{\circ}C$. Then, the variation in the strength value was observed, depending on the freezing environment. In addition, the tests divided the loading rate into 6 phases and observed the variation in the stress-strain ratio, depending on the loading rate. The test data showed that a lower freezing temperature resulted in a larger strength value. An increase in the ice content in the specimen with the increase in the water content ratio influenced the strength value of the specimen. A faster load rate had a greater influence on the uniaxial compression and indirect tensile strengths of a frozen specimen and produced a different strength engineering property through the initial tangential modulus of elasticity. Finally, the long-term strength under a constant water content ratio and freezing temperature was checked by producing stress-strain ratio curves depending on the loading rate.

An Assessment of a Resilient Modulus Model by Comparing Predicted and Measured Elastic Deformation of Railway Trackbeds (철도노반의 탄성변위 예측 및 측정을 통한 회복탄성계수 모델 평가)

  • Park, Chul-Soo;Kim, Eun-Jung;Oh, Sang-Hoon;Kim, Hak-Sung;Mok, Young-Jin
    • Proceedings of the Korean Geotechical Society Conference
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    • 2008.10a
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    • pp.1404-1414
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    • 2008
  • In the mechanistic-empirical trackbed design of railways, the resilient modulus is the key input parameter. This study focused on the resilient modulus prediction model, which is the functions of mean effective principal stress and axial strain, for three types of railroad trackbed materials such as crushed stone, weathered soil, and crushed-rock soil mixture. The model is composed with the maximum Young's modulus and nonlinear values for higher strain in parallel with dynamic shear modulus. The maximum values is modeled by model parameters, $A_E$ and the power of mean effective principal stress, $n_E$. The nonlinear portion is represented by modified hyperbolic model, with the model parameters of reference strain, ${\varepsilon}_r$ and curvature coefficient, a. To assess the performance of the prediction models proposed herein, the elastic response of a test trackbed near PyeongTaek, Korea was evaluated using a 3-D nonlinear elastic computer program (GEOTRACK) and compared with measured elastic vertical displacement during the passages of freight and passenger trains. The material types of sub-ballasts are crushed stone and weathered granite soil, respectively. The calculated vertical displacements within the sub-ballasts are within the order of 0.6mm, and agree well with measured values with the reasonable margin. The prediction models are thus concluded to work properly in the preliminary investigation.

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Earthquake Response Analysis of a Buried Gas Pipeline (매설가스배관의 지진응답해석)

  • Lee, Do-Hyung;Cho, Kyu-Sang;Chung, Tae-Young;Kong, Jung-Sik
    • Journal of the Earthquake Engineering Society of Korea
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    • v.11 no.6
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    • pp.41-52
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    • 2007
  • Earthquake time-history analyses have been carried out for a buried gas pipeline of X65 which is of popular use in Korea. Parameters included are shape of a buried gas pipeline, soil characteristics, single and multiple earthquake input ground motions and burial depths. Predicted response of strain and relative displacement are then compared with allowable strain and displacement capacity calculated by Guidelines for the Seismic Design of Buried Gas Pipelines, KOGAS. Comparative studies show that strains are in general affected by the burial depths together with change of soil conditions. Regarding the relative displacement, while axial relative displacement is not influenced by the burial depths, transverse relative displacement is affected by both burial depths as well as soil conditions. In all, the current study is encouraged to give a useful information for healthy earthquake evaluation of a buried pipeline.

Deformation Analysis of Geosynthetic Reinforced Retaining Wall by Using Temperature Dependent Confined Tension Test Results (온도제어 구속인장시험에 의한 토목섬유 보강토옹벽의 변위해석)

  • 김홍택;방윤경;조용권
    • Journal of the Korean Geotechnical Society
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    • v.19 no.2
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    • pp.97-106
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    • 2003
  • In this study, the effect of temperature and soil confining stress on geosyntheic stress-strain properties was quantified by performing the temperature dependent confined tension tests for four types of geosynthetic including woven geotextile, composite, geomembrane and geogrid specimen. Temperature instrumentation on the GRS-retaining wall constructed in Jaechon-shi area was also performed to examine the a seasonal temperature variation of geosynthetic reinforcements in the backfill. Based on the test results, a comparison was made between unconfined and confined moduli far each temperature to quantify the soil confinement and temperature effect on stress-strain properties. And it was also proposed that the simple expressions for the secant moduli of geosynthetics as a function of temperature and confining stress on geosynthetics. As a result of the FDM analysis of GRS-retaining wall, the method of considering the effect of temperature and confining stress on geosynthetic reinforcements when performing the FDM analysis of GRS-retaining wall was proposed.

Complete genome sequence of Gordonia sp. MMS17-SY073, a soil actinobacterium (토양 방선균인 Gordonia sp. MMS17-SY073 균주의 유전체 분석)

  • Kim, Yeong Seok;Kim, Seung Bum
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.303-305
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    • 2019
  • An actinobacterial strain designated Gordonia sp. MMS17-SY073 (=KCTC 49257) was isolated from a coastal soil of an island, and its complete genome was analyzed. A single contig consisting of 5,962,176 bp with the G + C content of 67.4% was obtained, and the annotation resulted in 5,201 protein-coding genes, 6 rRNA genes and 45 tRNA genes. Strain MMS17-SY073 was closest to the type strain of Gordonia soli based on the 16S rRNA gene sequence comparison, sharing 98.5% sequence similarity. A number of biosynthetic gene clusters for secondary metabolites, non-ribosomal peptide synthetase types in particular, could be identified from the genome.

Investigation on the responses of offshore monopile in marine soft clay under cyclic lateral load

  • Fen Li;Xinyue Zhu;Zhiyuan Zhu;Jichao Lei;Dan Hu
    • Geomechanics and Engineering
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    • v.37 no.4
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    • pp.383-393
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    • 2024
  • Monopile foundations of offshore wind turbines embedded in soft clay are subjected to the long-term cyclic lateral loads induced by winds, currents, and waves, the vibration of monopile leads to the accumulation of pore pressure and cyclic strains in the soil in its vicinity, which poses a threat to the safety operation of monopile. The researchers mainly focused on the hysteretic stress-strain relationship of soft clay and kinds of stiffness degradation models have been adopted, which may consume considerable computing resources and is not applicable for the long-term bearing performance analysis of monopile. In this study, a modified cyclic stiffness degradation model considering the effect of plastic strain and pore pressure change has been proposed and validated by comparing with the triaxial test results. Subsequently, the effects of cyclic load ratio, pile aspect ratio, number of load cycles, and length to embedded depth ratio on the accumulated rotation angle and pore pressure are presented. The results indicate the number of load cycles can significantly affect the accumulated rotation angle of monopile, whereas the accumulated pore pressure distribution along the pile merely changes with pile diameter, embedded length, and the number of load cycles, the stiffness of monopile can be significantly weakened by decreasing the embedded depth ratio L/H of monopile. The stiffness degradation of soil is more significant in the passive earth pressure zone, in which soil liquefaction is likely to occur. Furthermore, the suitability of the "accumulated rotation angle" and "accumulated pore pressure" design criteria for determining the required cyclic load ratio are discussed.

Protection of Tobacco Plants from Bacterial Wilt with Avirulent Bacteriocin-Producing Strains of Pseudomonas solanacearum (비병원성 Bacteriocin 생성 Pseudomonas solanacearum을 이용한 담배 세균성마름병 방제)

  • 이영근
    • Journal of the Korean Society of Tobacco Science
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    • v.13 no.2
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    • pp.42-47
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    • 1991
  • Control effect of an avirulent bacterlocin-producing strain(ABPS) Y6l-1 of Pseudomonas solanacearum on bacterial wilt was 58.8 % when the bacterial suspension had poured onto the rhizosphere soil of tobacco cultivar NC82 on one day before transplanting to the field and of hilling time. Until eight weeks after inoculation, survival of the strain on rhizoplane and in stem of the plants inoculated was better than that of other four strains tested. It suggested that survival of the ABPS in and on the plants should be supported for the sufficient protection.

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