• Proceedings of the PSK Conference
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• 2002.10a
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• pp.331.3-332
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• 2002

A novel penicillin G acylase (PGA)-producing bacterial strain was isolated from soil by using the Serratia marcescens overlay technique. The isolated strain was identified as Leclercia adecarboxylata based on the analyses of the biochemical characteristics (API 20E). the cellular fatty acid profile. and the 16S rDNA sequences. The gene encoding the PGA (pac gene) was cloned into the pHSG399 vector and the recombinant E. coliHB101 clones harboring the pac gene were isolated on agar plates containing phenylacetyl-L -leucine and penicillin G. (omitted)

• Korean Journal of Microbiology
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• v.30 no.6
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• pp.438-443
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• 1992

Thirteen strains of Bacillus thuringiensis were isolated from soil in Korea and characterized. The all strains produced parasporal crystals and spores in their cells. Two strains had bipyramidal crystals, seven strains contianed round ones and four strains had unregular ones. Only minor biochemical characteristics of the thirteen isolates were different and distinctive, however general characteristics were similar to the known serotypes of B. thuringiensis. Two strains were resistant to ampicilin. Three strains were resistant to bacitracin, six strains were resistant to cephalothin, two strains were resistant to colistin, HL-68 strain was resistant to gentamycin, HL-67 strain was resistant to kanamycin and HL-71 was resistant to tetracycline. Two strains were resistant to penicillin G. Four strains were toxic to Bombyx mori larvae and eleven strains were toxic to Culex pipiens larvae.

• Geotechnical Engineering
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• v.4 no.2
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• pp.45-55
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• 1988

Chemical grouting is one of the ground.improvement methods for the purpose of cutting o($\boxUl$ water and increasing the strength of soil. It has ben reported that the effect of strength increasement of groued roils is due to increase of cohesion. In this study, the effect of cohesion on the shearing resistance of grouted soil 9.as intr.estigated tall.ouch triaxial compression test. According to the result of this research, It is found that the improved cohesion increases rapidly up to the maximum value at a small strain and subsequent decrease of cohericn is due to the breaking of grout chemical at a larger strain.

• Journal of The Korean Society of Agricultural Engineers
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• v.47 no.6
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• pp.59-70
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• 2005

A series of resonant column test was performed to investigate the dynamic deformation characteristics of silty sand soils mixed with polypropylene fibrillated type fiber. Results show that optimum mixing ratios were $0.2\%$ for 19mm of cut fiber for shear modulus and $0.1\%$ for 60mm cut fiber fur damping ratio. As shear strain was increased, normalized values of shear modulus (G(Reinforced)/ G(Unreinforced)) of fiber reinforced soil were increased up to $10^{-3}\%\~10^{-1}\%$ ranges. However, normalized damping ratio (D(Reinforced/D(Unreinforced)) was diminished with an increase in strain beyond $10^{-3}\%\~10^{-1}\%$ for the damping capacity of soils mixed with fiber. Normalized shear modulus $(G/G_{max})$ obtained from the test was plotted in the chart suggested by Seed and Idriss. The shear modulus of silty sand was located between sand and gravel curves.

• Geomechanics and Engineering
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• v.14 no.4
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• pp.315-324
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• 2018

With rapid economic growth, numerous deep excavation projects for high-rise buildings and subway transportation networks have been constructed in the past two decades. Deep excavations particularly in thick deposits of soft clay may cause excessive ground movements and thus result in potential damage to adjacent buildings and supporting utilities. Extensive plane strain finite element analyses considering small strain effect have been carried out to examine the wall deflections for excavations in soft clay deposits supported by diaphragm walls and bracings. The excavation geometrical parameters, soil strength and stiffness properties, soil unit weight, the strut stiffness and wall stiffness were varied to study the wall deflection behaviour. Based on these results, a multivariate adaptive regression splines model was developed for estimating the maximum wall deflection. Parametric analyses were also performed to investigate the influence of the various design variables on wall deflections.

• Journal of Microbiology and Biotechnology
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• v.24 no.1
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• pp.13-18
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• 2014

Bacillus subtilis JW-1 was isolated from rhizosphere soil as a potential biocontrol agent of bacterial wilt caused by Ralstonia solanacearum. Seed treatment followed by a soil drench application with this strain resulted in >80% reduction in bacterial wilt disease compared with that in the untreated control under greenhouse conditions. The antibacterial compound produced by strain JW-1 was purified by bioactivity-guided fractionation. Based on mass spectroscopy and nuclear magnetic resonance spectral data ($^1H$, $^{13}C$, $^1H-^1H$ correlation spectroscopies, rotating frame nuclear Overhauser effect spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structure of this compound was elucidated as a cyclic lipopeptide composed of a heptapeptide (Gln-Leu-Leu-Val-Asp-Leu-Leu) bonded to a ${\beta}$-hydroxy-iso-hexadecanoic acid arranged in a lactone ring system.

• Proceedings of the Korean Geotechical Society Conference
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• 2002.10a
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• pp.643-650
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• 2002

It is necessary, in the light of the importance of long-term slope stability problem, to develop a simple method or tool which can figure out the possible failure zone resulted from weathering effect and other factors. The FBG sensor system is used to estimate the correlations between the temperature and the slope in Yunhwajae, and to find a failure zone in slopes effectively. This research is to seek for the correlation between the soil temperature distribution and the strain distribution in a active zone by analyzing the data from the in-situ measurement so that the possible failure zone should be well defined based on the correlation. The zone of high temperature fluctuation can be regarded as one of the possible sliding zone due to the weathering effect while the constant temperature depth of the ground, if exists, would not be relatively affected by the weathering process.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2003.03a
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• pp.140-147
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• 2003

There are many problems in the prediction of soil dynamic behaviors because undrained excess pore water pressure builds up and then the strain softening behavior is occurred simultaneously. A few analytical methods based on the dynamic constitutive model have been proposed but the model hardly predict the excess pore water pressure directly. In this study, the verification on the disturbed state concept (DSC) model, proposed by Dr, Desai was performed. Some laboratory tests such as conventional triaxial tests and cyclic triaxial tests were carried out to determine DSC Parameters and then disturbance values are determined by the proposed equation. Through this verification, it is proved that the disturbed state concept can express reliably the soil dynamic characteristics such as excess pore water pressure and strain softening behavior. It is also found that the critical disturbance which is determined at the minimum curvature of disturbance function can be a the specific index.

• Journal of Species Research
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• v.13 no.1
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• pp.61-66
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• 2024

During an investigation of unrecorded prokaryotic species in Korea, six unrecorded bacterial strains were isolated from soil samples collected from Uljin-gun. Based on a similarity search using the 16S rRNA gene sequence of the isolated strains and the construction of the neighbor-joining phylogenetic tree, five strains were identified to the genus Pseudomonas of the family Pseudomonadaceae, while one strain was identified as a species belonging to the genus Paenibacillus of the family Paenibacillaceae. The details of these unreported species, including gram staining reaction, colony and cell morphology, basic biochemical characteristics, strain ID, and isolation source, are described in the description of the strains.

• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1123-1132
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• 2013

The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing ability, with 100 mg/l Cr(VI) being completely removed within 144 h. It could effectively remove Cr(VI) over a wide pH range from 5.5 to 9.5, with the optimal pH of 8.5. The amount of Cr(VI) removed increased with initial Cr(VI) concentration. Data from the time-course analysis of Cr(VI) removal by strain LY6 followed first-order kinetics. Based on the 16S rRNA gene sequence, strain LY6 was identified as Pseudochrobactrum asaccharolyticum, a species that had never been reported for Cr(VI) removal before. Transmission electron microscopy and energy dispersive X-ray spectroscopy analysis further confirmed that strain LY6 could accumulate chromium within the cell while conducting Cr(VI) removal. The results suggested that the indigenous bacterial strain LY6 would be a new candidate for potential application in Cr(VI) pollution bioremediation.