• Korean Journal of Microbiology
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• v.49 no.4
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• pp.369-376
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• 2013

We isolated the ${\beta}$-glucosidase producing bacteria (BGB) in ginseng root system (rhizosphere soil, rhizoplane, inside of root). Phylogenetic analysis of the 28 BGB based on the 16S rRNA gene sequences, BGB from rhizosphere soil belong to genus Stenotrophomonas (3 strains), Bacillus (1 strain), and Pseudoxanthomonas (1 strain). BGB isolates from rhizoplane were Stenotrophomonas (16 strains), Streptomyces (1 strain) and Microbacterium (1 strain). BGB from inside of root were categorized into Stenotrophomonas (3 strains) and Lysobacter (2 strains). Especially, Stenotrophomonas comprised the largest portion (approximately 90%) of total isolates and Stenotrophomonas was a dominant group of the ${\beta}$-glucosidase producing bacteria. We selected strain 4KR4, which had high ${\beta}$-glucosidase activity (108.17 unit), could transform ginsenoside Rb1 into Rd, Rg3, and Rh2 ginsenosides. In determining its relationship on the basis of 16S rRNA sequence, 4KR4 strain was most closely related to Stenotrophomonas rhizophila e-$p10^T$ (AJ293463) (99.62%). Therefore, on the basis of these polyphasic taxonomic evidence, the ginsenoside Rb1 converting bacteria 4KR4 was identified as Stenotrophomonas sp. 4KR4 (=KACC 17635).

• Korean Journal of Microbiology
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• v.49 no.3
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• pp.297-300
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• 2013

EPS producing bacteria were enumerated in ginseng root system (rhizosphere soil, rhizoplane, inside of root). EPS producing bacterial density of rhizosphere soil, rhizoplane and inside of root were distributed $9.0{\times}10^6$ CFU/g, $7.0{\times}10^6$ CFU/g, and $1.4{\times}10^3$ CFU/g, respectively. Phylogenetic analysis of the 24 EPS producing isolates based on the 16S rRNA gene sequences, EPS producing isolates from rhizosphere soil (RS) belong to genus Arthrobacter (6 strains) and Rhizobium (1 strain). EPS producing bacteria from rhizoplane (RP) were Arthrobacter (6 strains), Rhodococcus (1 strain) and Pseudomonas (1 strain). EPS producing bacteria from inside of root (IR) were categorized into Rhzobium (6 strains), Bacillus (1 strain), Rhodococcus (1 strain), and Pseudomonas (1 strain). Phylogenetic analysis indicated that Arthrobacter may be a member of representative EPS producing bacteria from ginseng rhizosphere soil and rhizoplane, and Rhizobium is typical EPS producing isolates from inside of ginseng root. The yield of EPS was 10.0 and 4.9 g/L by Rhizobium sp. 1NP2 (KACC 17637) and Arthrobacter sp. 5MP1 (KACC 17636). The purified EPS were analyzed by Bio-LC and glucose, galactose, mannose and glucosamine were detected. The major EPS sugar of these strains was glucose (72.7-84.9%).

• The Plant Pathology Journal
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• v.31 no.2
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• pp.140-151
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• 2015

Bacillus subtilis B006 strain effectively suppresses the cucumber fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (Foc). The population dynamics of Foc, strain B006 and its surfactin over-producing mutant B841 and surfactin-deficient mutant B1020, in the rhizosphere were determined under greenhouse conditions to elucidate the importance of the lipopeptides excreted by these strains in suppressing Foc. Results showed that B. subtilis strain B006 effectively suppressed the disease in natural soil by 42.9%, five weeks after transplanting, whereas B841 and B1020 suppressed the disease by only 22.6% and 7.1%, respectively. Quantitative PCR assays showed that effective colonization of strain B006 in the rhizosphere suppressed Foc propagation by more than 10 times both in nursery substrate and in field-infected soil. Reduction of Foc population at the cucumber stems in a range of $0.96log_{10}ng/g$ to $2.39log_{10}ng/g$ was attained at the third and the fifth weeks of B006 treatment in nursery substrate. In field-infected soil, all three treatments with B. subtilis suppressed Foc infection, indicated by the reduction of Foc population at a range of $2.91log_{10}ng/g$ to $3.36log_{10}ng/g$ at the stem base, one week after transplanting. This study reveals that the suppression of fusarium wilt disease is affected by the effective colonization of the surfactin-producing B. subtilis strain in the rhizosphere. These results improved our understanding of the biocontrol mechanism of the B. subtilis strain B006 in the natural soil and facilitate its application as biocontrol agent in the field.

• Earthquakes and Structures
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• v.5 no.4
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• pp.417-438
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• 2013

This study was concerned on the application of a hybrid approach for analyzing the buried pipelines deformations subjected to earthquakes. Nonlinear time-history analysis of Finite Element (FE) model of buried pipelines, which was modeled using laboratory data, has been performed via selected earthquakes. In order to verify the FE model with experiments, a statistical test was done which demonstrated a good conformity. Then, the FE model was developed and the optimum intersection angle of pipeline and fault was obtained via genetic algorithm. Transient seismic strain of buried pipeline in the optimum intersection angle of pipeline and fault was investigated considering the pipes diameter, the distance of pipes from fault, the soil friction angles and seismic response duration of buried pipelines. Also, a two-layer perceptron Artificial Neural Network (ANN) was trained using results of FE model, and a nonlinear relationship was obtained to predict the bending strain of buried pipelines based on the pipes diameter, intersection angles of the pipelines and fault, the soil friction angles, distance of pipes from the fault, and seismic response duration; whereas it contains a wide range of initial input data without any requirement to laboratory measurements.

• Journal of the Korean Geotechnical Society
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• v.19 no.1
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• pp.77-92
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• 2003

A set of standard triaxial testing was performed to identify underlying physical processes and inherent limitations in the determination of critical state parameters in sandy soils. The experimental test results showed that the critical state friction angle for a given soil is constant regardless of drainage condition while the critical state line on the e-log p'space is significantly affected by drainage condition mainly because of insufficient strain attained in standard triaxial tests and strain localization effects in udrained tests. It appeared that the best method to determine critical state parameters in laboratory testing is to use homogeneous loose specimens under drained shear condition. In addition, a reference state parameter was suggested to design tests that will avoid dilatancy or strain localization effects in drained tests.

• Korean Journal of Environmental Biology
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• v.36 no.2
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• pp.190-198
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• 2018

The petroleum industry is an important part of the world economy. However, the massive exposure of petroleum in nature is a major cause of environmental pollution. Therefore, the microbial mediated biodegradation of petroleum residues is an emerging scientific approach used to resolve these problem. Through the screening of diesel contaminated soil we isolated a rapid phenanthrene and a diesel degrading bacterium identified as Enterobacter cancerogenus DA1 strain through 16S rRNA gene sequence analysis. The strain was registered in NCBI with an accession number MG270576. The optimal growth condition of the DA1 strain was determined at pH 8 and $35^{\circ}C$, and the highest degradation rate of the diesel was achieved at this condition. At the optimal condition, growth of the strain on the medium containing 0.05% phenanthrene and 0.1% of diesel-fuel was highest at 45 h and 60 h respectively after the incubation period. Biofilm formation was found significantly higher at $35^{\circ}C$ as compared to $30^{\circ}C$ and $40^{\circ}C$. Likewise, the lipase activity was found significantly higher at 48 h after the incubation compared to 24 h and 72 h. These results suggest that the Enterobacter cancerogenus DA1 could be an efficient candidate, for application through ecofriendly scientific approach, for the biodegradation of petroleum products like diesel.

• Mycobiology
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• v.45 no.4
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• pp.370-378
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• 2017

Cylindrocarpon destructans is an ascomycete soil-borne pathogen that causes ginseng root rot. To identify effective biocontrol agents, we isolated several bacteria from ginseng cultivation soil and evaluated their antifungal activity. Among the isolated bacteria, one isolate (named JH7) was selected for its high antibiotic activity and was further examined for antagonism against fungal pathogens. Strain JH7 was identified as a Chromobacterium sp. using phylogenetic analysis based on 16S rRNA gene sequences. This strain was shown to produce antimicrobial molecules, including chitinases and proteases, but not cellulases. Additionally, the ability of JH7 to produce siderophore and solubilize insoluble phosphate supports its antagonistic and beneficial traits for plant growth. The JH7 strain suppressed the conidiation, conidial germination, and chlamydospore formation of C. destructans. Furthermore, the JH7 strain inhibited other plant pathogenic fungi. Thus, it provides a basis for developing a biocontrol agent for ginseng cultivation.

• Journal of the Korean Geotechnical Society
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• v.26 no.11
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• pp.39-46
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• 2010

The magnitude of the lateral resistance that resists the lateral movement of the pile is controlled by the amount of the pile movement and the strength and stiffness of soil. In this paper, we proposed an equation which produces the lateral resistance of the laterally loaded single pile using the strain wedge model of the soil deformation. The results of this equation is compared with results of model test, field test, p-y curve and other methods. It is found that the result of proposed equation is smaller than the result of model test. The results of loading test considerably coincide with those of proposed equation; however, a few of deviations are generated as the displacement of pile head increases. Moreover, coincidences exist between the results of the proposed equation and those of finite difference method.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.1047-1054
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• 2005

The purpose of this study is the understanding to changes in the characteristic of soil structure and classification, atterberg limits, undrained shear strength and consolidation of clayey soil dependent on pH of soil pore water. A series of tests including consistency tests, uniaxial compressive tests, vane tests and oedometer tests are performed on. The test results indicated that pH changes in the soil pH resulted in changes in the soil structure and classification, stress-strain behavior. Specially, when pH is conditioned to 7, liquid limit, undrained shear strength and preconsolidation pressure are the largest.

• Magazine of the Korean Society of Agricultural Engineers
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• v.44 no.6
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• pp.115-123
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• 2002

This study was performed to provide basic data for development and construction of reinforced soil wall that mixed with reinforcements such as calcium carbonate, monofilament fiber. In order to determine proper moisture content and mixing ratio by weight of reinforcement, Poisson's ratio and compressive strength tests for sandy soil had been conducted. Model tests for long-term behavior of reinforced soil wall were carried out to investigate the effect of reinforcement during loads and under static loads. The results of creep and model tests for sandy soil compared with clayey soil. Reinforced sandy soil mixed with calcium carbonate and cement showed brittle rupture by shear but that of mixed with monofilament fiber showed ductile rupture due to the tension force of fiber. It was shown that when age increased, creep strain of reinforced soil under sustained load approached constant values.