• Korean Journal of Microbiology
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• v.15 no.4
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• pp.159-169
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• 1977

The bacteria of red colonies isolated from soil were identified as Serratia marcescens. The best solvent for pigment extraction was n-buthanol and the pigment was identified as prodigiosene. The extracted pigment was stable on temperature and light but not on acidity. The redpigment color changed into red in alkaline solution. The maximum absorbancy of pigment was 466 nm in alkaline condition and 540 nm in acid condition. And the pigment formed single spot on the TLC(starch). By the result of infra red spectrum, the red pigment has the same absorption pattern comparing with, the prodigisin produced by S. marcescens strain Nima. It was confirmed that the pigment was secondary metabolite and that the maximal peak of production appeared at 30 hrs after the inoculation, when the bacterial growth was in statinary state. Referring to the effect of temperature, the pigment was not formed at $36^{\circ}C$ and the optimal temperature for both of bactrial growth and pigmentation was $30^{\circ}C$. The optimal range of pH for pigmentation was 5.0 and under the condition the bacterial growth was not affected at all. Examining the effects of light, the bacterial pigment ation was more increased in darkness than in visible light.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2001.04a
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• pp.387-393
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• 2001

Installing vibration control devices in the structure rises as a solution instead of increasing structural strength considering construction cost. Especially, viscoelastic dampers show excellent vibration control performance at low cost and are easy to install in existing structures compared with other control devices. Therefore, cost-effectiveness of structure with viscoelastic dampers needs to be evaluated. Previous cost-effectiveness evaluation method for the seismically isolated structure(Koh et al., 1999;2000)is applied on the building structure with viscoelastic dampers, which combines optimal design and cost-effectiveness evaluation for seismically isolated structures based on minimum life-cycle cost concept. Input ground motion is modeled in the form of spectral density function to take into account acceleration and site coefficients. Damping of the viscoelastic damper is considered by modal strain energy method. Stiffness of shear building and shear area of viscoelastic damper are adopted as design variables for optimization. For the estimation of failure probability, transfer function of the structure with viscoelastic damper for spectral analysis is derived from the equation of motion. Results reveal that cost-effectiveness of the structure with viscoelastic dampers is relatively high in how seismic region and stiff soil condition.

• Journal of the Korean Geotechnical Society
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• v.16 no.4
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• pp.191-199
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• 2000

In this research, the finite element analysis of piezocone penetration and dissipation tests has been conducted using the anisotropic elastoplastic-viscoplastic bounding surface model, virtual work equation, and theory of mixtures formulated in the Up[dated Lagrangian reference frame for the large deformation and finite strain nature of piezocone penetration. The formulated equations have been implemented into a finite element program. The cone resistance, excess pore water pressure, and dissipation of excess pore water pressure from the finite element analysis have been compared and investigated. An effective simulation could be performed with the use of the anisotropic and viscous soil model. The finite element formulations and the results are described in part 'I' and part 'II' respectively.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.607-614
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• 2010

Shear behavior of granular soils largely affects the safety and stability of underground and earth structures. This study presents the characteristics of shear zone in a direct shear test using shear wave and electrical resistivity measurements. An innovative direct shear box made of transparent acrylic material has been developed to prevent direct electric current. Bender elements and electrical resistivity probe are embedded in the wall of direct shear box to estimate the shear wave velocities and the electrical resistivity at the shear and non-shear zones. Experimental results show that the void ratio and shear wave velocity at shear zone increase during shearing while the values remain constant at non-shear zone. The results demonstrate correlation among the contact force, small strain shear modulus, and void ratio at shear zone. This study suggests that the application of the modified direct shear box including shear wave and electrical resistivity measurements may become an effective tool for analyzing soil behavior at shear zone.

• Journal of the Korean Geotechnical Society
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• v.27 no.5
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• pp.17-31
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• 2011

This paper presents the results of a numerical investigation on the behavior of earth retaining wall system with emphasis on the groundwater lowering. Using the 2D stress-pore pressure coupled analysis, the effects of ground excavation and groundwater interaction were examined using wall horizontal deformation, ground surface movement, plastic strain pattern, effective stress distribution and axial stress of strut. In addition, based on the results from a parametric study on a wide range of soil profile and initial ground water table level, the ranges of wall displacement and ground deformation were suggested quantitatively.

• Proceedings of the Korean Geotechical Society Conference
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• 2006.03a
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• pp.590-599
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• 2006

In this study, Liquefaction characteristics of saturated sand under various dynamic loadings such as sinusoidal loading, increasing wedge loading, and real earthquake loading were investigated focusing on the dissipated energy. From the results of cyclic triaxial test, liquefaction resistance strength was calculated by the concept of energy according to the type of input loading. Liquefaction resistance strength was expressed in accumulated dissipated energy calculated from stress-strain curve(hysteresis loop). The dissipated energy according to loading type was compared and the energy-based evaluation was proposed. The procedures are presented in terms of normalized energy demand(NED), normalized energy capacity(NEC), and factor of safely, where NED is the load imparted to the soil by the loading(both amplitude and duration), NEC is the demand required to induce liquefaction, and factor of safely is defined as the ratio of NEC and NED.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.982-989
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• 2005

In this study, a series of laboratory tests based on 'Unit-cell concept' are performed to investigate improvement characteristics of clay ground in sand compaction pile method. Settlement reduction characteristics of composite ground and improvement characteristics of clay part could be qualified. In these procedure, the new strain-compression index($C_{\epsilon}$) of composite ground are adopted to show compressibility of composite ground according to the area replacement ratio, which is similar to the compression index($C_c$) in pure clay ground. Also, using normalization of reduction of water content in composite ground to the initial water content, improvement characteristics of clay part are investigated.

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.69-75
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• 2004

Optimal protocol for efficient genetic transformation has been defined to aid future strategies of genetic engineering in pigeon pea with agronomically important genes. Transgenic pigeonpea plants were successfully produced through Agrobacterium tumefaciens-mediated genetic transformation method using cotyledonary node explants by employing defined culture media. The explants were co-cultivated with A. tumefaciens strain C-58 harboring the binary plasmid, pCAMBIA-1301 [con-ferring $\beta$-glucuronidase(GUS) activity and resistance to hygromycin] and cultured on selection medium (regeneration medium supplemented with hygromycin) to select putatively transformed shoots. The shoots were then rooted on root induction medium and transferred to pots containing sand and soil mixture in the ratio of 1:1. About 22 putative TO transgenic plants have been produced. Stable expression and integration of the transgenes in the putative transgenics were confirmed by GUS assay, PCR and Southern blot hybridization with a transformation efficiency of over 45%. Stable integration and expression of the marker gene has been confirmed in the TO and T1 transgenics through PCR, and Southern hybridization.

• The Korean Journal of Food And Nutrition
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• v.22 no.1
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• pp.97-102
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• 2009

In order to obtain a non-toxic and more active and stable microorganism-produced tyrosinase inhibitor, we isolated actinomycetes F-97, a producer of tyrosinase inhibitor, from soil. The aerial hyphae of this strain were gray in color with tree types. Under the microscopic examination, the isolate formed a spiral aerial spore mass with a smooth surface. The analysis of cell wall acid hydrolysate of the isolate revealed the presence of LL-diaminopimelic acid(LL-DAP). No specific sugar was detected. From these results and the cultural and physiological characteristics described in the Bergey's Manual, actinomycetes F-97 was identificated as, or best-matched to, Streptomyces aburaviensis.

• Geomechanics and Engineering
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• v.8 no.5
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• pp.707-726
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• 2015

This paper investigates the time dependent behaviour of Haarajoki test embankment on soft structured clay deposit. Half of the embankment is constructed on an area improved with prefabricated vertical drains, while the other half is constructed on the natural deposit without any ground improvement. To analyse the PVD-improved subsoil, axisymmetric vertical drains were converted into equivalent plane strain conditions using three different approaches. The construction and consolidation of the embankment are analysed with the finite element method using a recently developed anisotropic model for time-dependent behaviour of soft clays. The constitutive model, namely ACM-S accounts for combined effects of plastic anisotropy, interparticle bonding and degradation of bonds and creep. For comparison, the problem is also analysed with isotropic Soft Soil Creep and Modified Cam Clay models. The results of the numerical analyses are compared with the field measurements. The results show that neglecting effects of anisotropy, destructuration and creep may lead to inaccurate predictions of soft clay response. Additionally, the numerical results show that the matching methods accurately predict the consolidation behaviour of the embankment on PVD improved soft clays and provide a useful tool for engineering practice.