• Journal of the Korean Geosynthetics Society
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• v.11 no.2
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• pp.1-9
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• 2012

In order to review the utility of Lade's single hardening constitutive model, a series of isotropic compression-expansion tests and consolidated drained triaxial tests including as CTC, TC, RTC, and OSP were performed by Baekma river sand with various of stress path. Parameters required in model were determined using these tests. The accuracy of analysis was reviewed by back analysis of test results used to determine the 11 parameters of soil property through the test of each stress path. Also. for verifying the accuracy of prediction for the stress-strain behavior using failure criterion related 9 parameters with correlational equation and constant and yield criterion related parameters h, ${\alpha}$ and ${\eta}_1$, when stress path is different with each other, it has been obtained in the review result of stress path dependent characteristics of the constitutional model through the analyzing results of CTC, TC, RTC, OSP, and fine silica sand tests.

• Journal of the Earthquake Engineering Society of Korea
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• v.14 no.5
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• pp.65-76
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• 2010

In this paper, earthquake fragility analysis has been comparatively performed with regard to a buried gas pipeline of API X65 which has been widely used in Korea. For this purpose, a nonlinear time-history analyses has been carried out for 15 different analytical models of a buried gas pipeline in terms of the selected 12 sets of earthquake ground motions with 0.1g of scaling interval. Following that, earthquake fragility analyses have been conducted using the maximum axial strain of the pipeline obtained from the nonlinear time-history analyses. Parameters under consideration for subsequent earthquake fragility analyses are soil conditions, end-restraint conditions, burial depth and the type of pipeline. Comparative analyses reveal that whereas the first three parameters influence the fragility curves, particularly soil conditions amongst the three parameters, the last parameter has a little effect on the curves. In all, the present study can be considered as a benchmark fragility analysis of a buried gas pipeline in the absence of an earthquake fragility analysis of the pipeline and thus is expected to be a useful source regarding earthquake fragility analyses of a buried gas pipelines.

• Geotechnical Engineering
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• v.12 no.6
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• pp.51-64
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• 1996

After the model open-ended pile attached with strain gages was driven into a pressure chamber, in which the saturated microfine sand was contained, the static compression loading test was performed for that pile. Based on the test results, ultimate pile capacity was determined. Then, either simulated earthquake shaking or sinusoidal shaking was applied to the pile with the sustained certain level OP ultimate pile load. Then, pile capacity degradations characteristics during shaking were studied. Pile capacity degradation during two different shakings were greatly different. During the simulated earthquake shaking, capacity degradation depended upon the magnitude of applied load. When the load applied to the pile top was less than 70% of ultimate pile capacidy, pile capacity degradation rate was less than 8%, and pile with the sustained ultimate pile load had the degradation rate of 90%. Also, most of pile capacity degradation was reduced in outer skin friction and degradation rate was about 80% of ultimate pile capacity reduction. During sinusoidal shaking, pile capacity degradation did not depend on the magnitude of applied load. It depended on the amplitude and the frequency , the larger the amplitude and the fewer the frequency was, the higher the degradation rate was. Reduction pattern of unit soil plugging (once depended on the mode of shaking. Unit soil plugging force by the simulated earthquake shaking was reduced in the bottom 3.0 D, of the toe irrespective of the applied load, while reduction of unit soil plugging force by sinusoidal shaking was occurred in the bottom 1.0-3.0D, of the toe. Also, the soil plugging force was reduced more than that during simulated earthquake shaking and degradation rate of the pile capacity depended on the magnitude of the applied load.

• Korean Journal of Soil Science and Fertilizer
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• v.21 no.2
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• pp.129-134
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• 1988

Enzymatical properties of Streptomyces sp. S-45 producing chitinase and ${\beta}$-1.3-glucanase isolated from soil were investigated. Chitinase activity was 3.01(U/ml) and ${\beta}$-1.3-glucanase activity was 2.49(U/ml). The optimum medium for mycolytic enzyme production of strain was composed of 0.7% colloidal chitin, 0.3% glucose, 0.5% asparagine, 0.2% peptone, 0.01% NaCl, 0.01% $K_2HPO_4$ and 0.01% $MgSO_4{\cdot}7H_2O$ in intial pH 7.0. The optimal condition for mycolytic enzyme activities were: pH 6.5-7.0, $45-50^{\circ}C$. Enzyme activities were activated by metal ion as $10^{-2}M\;Co^{{+}{+}}$, $Cu^{{+}{+}}$, $Mn^{{+}{+}}$, $Al^{{+}{+}{+}}$ and $10^{-3}M\;Sn^{{+}{+}}$ but $Ag^{{+}{+}}$, $Hg^{{+}{+}}$ inhibited.

• Journal of Korean Society of Forest Science
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• v.65 no.1
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• pp.43-47
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• 1984

Pinus rigida ${\times}$ P. taeda seedlings in a nursery was inoculated with basidiospores of Pisolithus tinctorius (Pt) either collected from Suweon, Korea or introduced from U.S.A. to compare the effectiveness of the spores from two different origins as mycorrhizal inocula. Nursery beds were fumigated with methyl bromide and 1g of spores was used to inoculate $1m^2$ of soil surface just before seed sowing. Seedlings inoculated with American Pt (#250 strain from Georgia, U.S.A.) were 15% taller than Korean Pt at the end of the first growing season. The seedlings from fumigation treatment only (no inoculation involved) was slightly taller (statistically unsignificant) than those with Korean Pt, but slightly smaller than those with American Pt. In a subsequent year experiment, the seedlings inoculated with American and Korean Pt after soil fumigation were 66% and 60% taller, respectively, than seedlings infected by natural fungi without soil fumigation, suggesting the dual effects of Pt and fumigation on the seedling growth. Therefore potential of Pt spores for an effective inoculum exists and selection of Pt strains which have adapted to specific local environments is needed to develop better sources of mycorrhizal inocula.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.198-206
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• 1981

A field research was conducted to evaluate the effects of inoculation of Rhizobium meliloti and lime application on the early growth of alfalfa, var. Luna, in an acid soil. Alfalfa seeds were inoculated by lime-peat pelleting method with $A_3$ strain isolated from alfalfa roots. Both inoculated and noninoculated seeds were sown in acid soil (pH 5.5) with three levels of lime application (1, 3, 6 ton/ha) on mid-April. Inoculated seeds produced many effective nodules in early stage and increased nitrogen and chlorophyll contents of leaf, consequently stimulating alfalfa growth. Pseudo nodules were found in noninoculated, while they were seldom formed in inoculated. Lime application increased soil pH, plant height, root length, and shoot and root weight. Liming effect on nodulation was significant at first cut, but no longer after then. Both inoculation and liming increased the forage yield of alfalfa. Inoculation and liming of 6 ton/ha together produced the highest yield. As compared to control plot of nitrogen application, the effect of seed inoculation only could be equal to about 120 kg/ha nitrogen application. The total nitrogen contents of forage were little affected by number or weight of nodules and amounts of lime application.

• Geotechnical Engineering
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• v.12 no.6
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• pp.127-138
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• 1996

The in-situ consolidation behavior of drainage system-installed deposits has three dimensional characteristics. Therefore, for an approximate 2-D plane strain consolidation analysis, it is necessary to convert the 3-D spatial flow of actual cases into the laminar flow simulated by the 2-D plane strain model. . In this paper, in order to properly model the effect of three dimensional characteristics, an equivalent and efficient model has been applied in a finite element technique for the analysis of the drainage system-installed soil deposits. The equivalent two dimensional model involves equivalent permeabilities and drainage widths. To validate the equivalent two dimensional model, three dimensional analyses were per formed by using the ABAQUS program and the results of 3-D analyses were compared with those of the 2-D analyses. By using the proposed equivalent model, one may be able to appropriately predict the consolidation behavior of drainage system-installed soft deposits.

• Journal of the Korean Geotechnical Society
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• v.15 no.4
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• pp.133-152
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• 1999

The cyclic triaxial test was carried out to research liquefaction characteristics and sample disturbance effects of silty sand soils at the west coast in Korea. First, liquefaction in silty sand was generated when axial strain approached to $\pm10%$ of strain and behavior of pore pressure was similar to the formula suggested by Seed, Martin, and Lysmer(1975). Also, it was found that dilatancy was generated at failure. Secondly, the liquefaction evaluation methods suggested by many researchers were carried out and the results were compared. In these methods the weak depth in liquefaction was similar and the method carried out by cyclic triaxial test on remolded sample showed the least safety factor. Thirdly the stress ratio by cyclic triaxial test was compared with that obtained from SPT N-value as a kind of empirical methods. It was found that the effect of sample disturbance was relatively small when SPT N-value was less than 20, but there were large differences in safety factor and resistance of liquefaction in soil by the effects of disturbance and remolding when SPT N-value was more than 20.

• Korean Journal of Microbiology
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• v.21 no.2
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• pp.51-60
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• 1983

Cultural characteristics of Strptomyces griseolus isolated from the soil were investigated. This strain was disclosed to utilize D-xylose, and D-glactose in preference order as a carbon source with the formation of glucose isomerase. The addition of sweet potato starch also proved effective promoting the total enzyme activity measured at 29% higher than the control. Corn cob, one of waste agricultural resources, was hydrolyzed in 2~3% $H_2SO_4$ solution at $100^{\circ}C$, 3~5 hours to produce a xylose syrup which gave rise to the recovery of 19.9% in a batch system and 28.2% in a repeated system. By the addition of both 2% of xylose syrup(Be'28) prepared by and us 65% of corn steep liquor (total nitrogen 1.2%), enzyme induction was maximized. The enzyme activity was stimulated by the xylose and the cell growth by the C.S.L. Also, remarkable increase of enzyme activity was noticed by the addition of protein acid hydrolysate 86.2% higher than the control. $QO_2$ of the biomass cultured in 30L capacity jarfermentor recorded low oxygen requirement of 251.2 1/hr. Maximum activity of glucose isomerase was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery phase of the biomass growth. Glucose isomerase from the strain was activated by adding the $Co^{++}\;and\;Mg^{++}$ with optimum temperature of $73^{\circ}C$ and pH of 7.2. Conversion ratio of 60% glucose to frutose was 42.5% after 70 hours reaction.

• Plant Biotechnology Reports
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• v.5 no.2
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• pp.147-156
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• 2011

Efficient Agrobacterium-mediated genetic transformation of Scoparia dulcis L. was developed using Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pCAMBIA1301 with ${\beta}$-glucuronidase (GUS) (uidA) and hygromycin phosphotransferase (hpt) genes. Two-day precultured leaf segments of in vitro shoot culture were found to be suitable for cocultivation with the Agrobacterium strain, and acetosyringone was able to promote the transformation process. After selection on shoot organogenesis medium with appropriate concentrations of hygromycin and carbenicillin, adventitious shoots were developed on elongation medium by twice subculturing under the same selection scheme. The elongated hygromycin-resistant shoots were subsequently rooted on the MS medium supplemented with $1mg\;l^{-1}$ indole-3-butyric acid and $15mg\;l^{-1}$ hygromycin. Successful transformation was confirmed by PCR analysis using uidA- and hpt-specific primers and monitored by histochemical assay for ${\beta}$-GUS activity during shoot organogenesis. Integration of hpt gene into the genome of transgenic plants was also verified by Southern blot analysis. High transformation efficiency at a rate of 54.6% with an average of $3.9{\pm}0.39$ transgenic plantlets per explant was achieved in the present transformation system. It took only 2-3 months from seed germination to positive transformants transplanted to soil. Therefore, an efficient and fast genetic transformation system was developed for S. dulcis using an Agrobacterium-mediated approach and plant regeneration via shoot organogenesis, which provides a useful platform for future genetic engineering studies in this medicinally important plant.