• Geomechanics and Engineering
• /
• v.24 no.4
• /
• pp.337-348
• /
• 2021

Brillouin Optical Frequency Domain Analysis (BOFDA) is a distributed fiber optic sensing (DFOS) technique that has unique advantages for performance monitoring of piles. However, the complicated production process and harsh operating environment of offshore PHC pipe piles make it difficult to apply this method to pile load testing. In this study, sensing cables were successfully pre-installed into an offshore PHC pipe pile directly for the first time and the BOFDA technique was used for in-situ monitoring of the pile under axial load. High-resolution strain and internal force distributions along the pile were obtained by the BOFDA sensing system. A finite element analysis incorporating the Degradation and Hardening Hyperbolic Model (DHHM) was carried out to evaluate and predict the performance of the pile, which provides an improved insight into the offshore pile-soil interaction mechanism.

• Microbiology and Biotechnology Letters
• /
• v.52 no.2
• /
• pp.208-210
• /
• 2024

Spirosoma sp. SC4-14 was isolated from the rhizosphere soil of pepper (Capsicum annuum L.). The genome of the strain SC4-14 consists of a single chromosome with one plasmid, total size of 8,104,799 bp and the DNA G+C-content is 48.2%. This chromosome contains 6,479 protein-coding sequences and 41 tRNA and 6 rRNA genes.

• Journal of the Korean Geotechnical Society
• /
• v.26 no.7
• /
• pp.49-58
• /
• 2010

This paper presents experimental results of a series of 1-g shaking table model tests performed on end-bearing single piles and pile groups to investigate the effect of particle size on the dynamic behavior of soil-pile systems. Two soil-pile models were tested twice: first using Jumoonjin sand, and second using Australian Fine sand. In the case of single-pile models, the lateral displacement was almost within 1% of pile diameter which corresponds to the elastic range of the pile. The back-calculated p-y curves show that the subgrade reaction of the Jumoonjin-sand-model ground was larger than that of the Australian Fine-sand-model ground at the same displacement. This phenomenon means that the stress-strain behavior of Jumoonjin sand was initially stiffer than that of Australian Fine sand. This difference was also confirmed by resonant column tests and compression triaxial tests. And the single pile p-y backbone curves of the Australian fine sand were constructed and compared with those of the Jumoonjin sand. As a result, the stiffness of the p-y backbone curves of Jumunjin sand was larger than those of Australian fine sand. Therefore, using the same p-y curves regardless of particle size can lead to inaccurate results when evaluating dynamic behavior of soil-pile system. In the case of the group-pile models, the lateral displacement was much larger than the elastic range of pile movement at the same test conditions in the single-pile models. The back-calculated p-y curves in the case of group pile models were very similar in both sands because the stiffness difference between the Jumoonjin-sand-model ground and the Australian Fine-sand-model ground was not significantly large at a large strain level, where both sands showed non-linear behavior. According to a series of single pile and group pile test results, the evaluation group pile effect using the p-multiplier can lead to inaccurate results on dynamic behavior of soil-pile system.

• KSBB Journal
• /
• v.23 no.3
• /
• pp.219-225
• /
• 2008

For the production of an antifungal compound, one strain (I-8) was selected from approximately 400 strains isolated from various soil samples. The optimum carbon source, nitrogen source and pH culture conditions for the production of the antifungal compound were investigated. ISP No. 2 medium (yeast extract 0.4%, malt extract 1% and dextrose 0.4%, at pH 8) was determined to be the optimum medium. Strain I-8 showed broad antifungal activity against the plant pathogenic fungi tested, including Sclerotinia sclerotiorum KACC 41065, as well as cellulase and chitinase activities in an agar plate assay. The extraction of antifungal compounds was performed using ethyl ether and ethyl acetate. In a culture broth of strain I-8, the ethyl acetate extract exhibited effective growth inhibition against 14 of the 20 phytopathogenic fungi tested. By mixing the ethyl acetate extract from I-8 with the ethyl ether extract from the fungus 13-16, which shows specific antifungal activity against Colletotrichum orbiculare KACC 40808, the antifungal activity of I-8 against phytopathogenic fungi was confirmed to be slightly increased. Strain I-8 showed strong growth inhibition against 16 phytopathogenic strains in agar plate tests.

• Microbiology and Biotechnology Letters
• /
• v.33 no.1
• /
• pp.65-69
• /
• 2005

A bacterial strain AE-1-3, isolated from soil and wastewater identified as Pseudomonas strain, removed $NH_4^+\;and\; NO_3^-$ simultaneously in anaerobic cultivation in a medium containing $0.1\%\;NH_4NO_3\;and\;3.0\%$ glucose. The strain removed $NH_4^+\;,\;NO_3^-\;and\;NO_2^-$ completely in 15 days of anaerobic cultivation. Though N03- removed completely, $33\%\;of\;NH_4^+$ remained in 15 day of incubation in $1\%$ glucose and $0.1\%\;NH_4NO_3$ medium. The bacterium could remove $0.1\%\;NH_4NO_3$ completely in a short time by addition of $Cu^{2+},\;Zn^{2+},\;Sn^{2+}\;in\;0.5\%$ glucose medium. By chaning the metal concentration, $0.3\%\;NH_4NO_3$ could be removed completely.

• Applied Biological Chemistry
• /
• v.37 no.4
• /
• pp.221-225
• /
• 1994

For producing single cell protein from the agricultural waste, heat treatment and antibiotics on the growth of Cellulomonas sp. KL-6, isolated in rotting leaf and the adjacent soil mixture, were examined. The organism was able to grow until 5 min. at $65^{\circ}C$, 1 min. at $75^{\circ}C$ and 1/4 min. at $85^{\circ}C$ in gradually rising temperatures. It can be Seen that preheating the suspension at $48^{\circ}C$ results in a marked decrease in heat resistance. On heating at temperature of $55^{\circ}C$ for 30 min., strain KL-6 was more resisted in the 0.1 M phosphate buffer when such substrates as casamino acid (1%), yeast extract (1%) or xylose (5%) were added to it whereas this organism was appeared weaker resistances in 0.1 M phosphate buffer when cysteine (0.03 M), sodium citrate (1%) or casein (1%) were in fused into it. Test strain was susceptible to penicillin-G $(1.563\;{\mu}g/ml)$ and ampicillin $(3.125\;{\mu}g/ml)$, but the organism was resisted to kanamycin $(>200\;{\mu}g/ml)$. The treatment of strain KL-6 with sodium dodecyl sulfate (SDS) resulted in the elimination of R-plasmid from the host strain and the elimination rate with SDS $(10{\sim}30\;{\mu}g/ml)$ was about $9.2{\sim}31.2%$, respectively.

• Journal of the Korean Geotechnical Society
• /
• v.27 no.6
• /
• pp.17-26
• /
• 2011

Ground anchor method is one of the most popular reinforcing technology in Korea. For the sound monitoring of slope reinforced by permanent anchor for a long period, monitoring the tension force of ground anchor is very important. However, special technology except conventional load cell has not been developed for this purpose. In this paper, a new method is described to replace the conventional strain gauge and V.W. type load cell which has been commonly used as a prestress force monitoring tool for a short-term and long-term. Four 11.5 m long strain detectable tension type anchors were made using FBG sensor embedded tendon since FBG sensor is smaller than strain gauge type load cell and does not have noise from electromagnetic wave. Each two set strain detectable tension type anchors were installed into the different ground conditions, i.e., soft rock and weathered granite soil. Prestress force of ground anchor was monitored during the loading-unloading step from in-situ pullout test using proposed FBG sensor embedded in the tendon and the conventional load cell Test results show that the prestress force monitored from FBG sensor may well be used practically, for it almost matches with that measured from expensive load cell.

• Journal of Plant Biotechnology
• /
• v.36 no.1
• /
• pp.18-22
• /
• 2009

This work was conducted to establish an efficient plant regeneration system for genetic transformation and the in vitro conservation of Sedum sarmentosum genetic resources. Effects of glutamine and $AgNO_3$ on plant regeneration between two genotypes were investigated using MS media supplemented with 0.2 mg/L NM and 3.0 mg/L BA. Calluses were formed on leaf explants placed on MS solid media supplemented with 3 mg/L 2,4-D and 1 mg/L BA. Calluses of Keumsan local strain produced shoots at a frequency of up to 100% after 50 days of culture on medium supplemented with glutamine. The highest number of shoots per callus was 17.6 at 350 mg/L glutamine. However, calluses of Wanju local strain gave rise to no shoots under the same culture conditions. Likewise, calluses of Keumsan local strain produced shoots at a frequency of up to 100% after 50 days of culture on medium supplemented with $AgNO_3$ whereas Wanju local strain sporadically produced shoots. The highest number of shoots per callus of Keumsan local stain was 16.1 at $15{\mu}M$ $AgNO_3$. Regenerated shoots were subcultured on hormone-free MS medium for rooting and shoot growth, and then 3-5 cm high plantlets were transplanted to the artificial soils comprising vermiculite and perlite, where they survived at a frequency of 88-100%. After being transplanted into upland soil:sand (1:1, v/v) in a greenhouse, regenerated plants showed a morphologically normal growth.

• Geotechnical Engineering
• /
• v.13 no.5
• /
• pp.89-100
• /
• 1997

It is very improtant to evaluate the reliable nonlinear deformational characteristics of soils not only in the analysis of geotechnical structures under working stress conditions but also for the soil dynamic problems. Field testings such as crosshole and pressuremeter tests can be used to determine the modulus of soils under in-situ conditions, but it is not possible to determine the modulus over the entire strain amplitude range. Laboratory methods such as resonant column 1 torsional shear test can be used to determine the modulus over the whole strain amplitude range, but it is very difficult to obtain the representative undisturbed samples on the sixte. For the reliable evaluation of nonlinear deformation characteristics of soils on a typical site, small strain modulus obtained from field testy and nomalized modulus reduction curve determined by laboratory bests need to be combined. In this paper, laboratory and Held testy were performed at a sixte which consisted of granite wearthered residual boils to evaluate the nonlinear deformational characteristics of coils such as the effects of strain amplitude, loading frequency, confining pressure and sample disturbance. It has been shorn that when the effects of these factors are properly taken into account, the stiffness values evaluated by various field and labrotary tests are comparable to each other fairly well. Finally, the procedure to evaluate the nonlinear deformstional characteristics of the sixte was proposed.

• Journal of the Korean GEO-environmental Society
• /
• v.7 no.6
• /
• pp.13-22
• /
• 2006

The geotextile have been used in filtration and drainage for over 30 years in many applications of civil and environmental projects. Geotextile tube is compound technology of filtration and drainage property of geotextile. Geotextile have been used for various types of containers, such as small hand-filled sandbags, 3-dimensional fabric forms for concrete paste, large soil and aggregate filled geotextile gabion, prefabricated hydraulically filled containers, and other innovative systems involving containment of soils using geotextile. They are hydraulically filled with dredged materials. It have been applied in coastal protection and scour protection, dewatering method of slurry, and isolation of contaminated material. Recently, geotextile tube technology is no longer alternative construction technique but suitable desired solution. This paper presents the behavior of geotextile tube composite structure by 2-D limit equilibrium and plane strain analysis. 2-D limit equilibrium analysis was performed to evaluate the stability of geotextile tube composite structure for the lateral load and also the plane strain analysis was conducted to determine the design and construction factors. Based on the results of this paper, the three types of geotextile tube composite structure is stable. And the optimum tensile strength of geotextile is 151kN/m and maximum pumping pressure is 22.7kN/m.