• Geotechnical Engineering
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• v.14 no.5
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• pp.111-128
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• 1998

An experimental study, which included four 305mm-diameter test shafts, one reference shaft with standard design and three test shafts with isolation tubes, is described. The soil was also soil heave and shrinkage that occur during suction changes at the field site. The test shafts were monitored for a period of about 18 months. Maximum ground movements exceeding 35mm were observed. Movements of only 1 to 2mm were observed in the test shafts with isolation tubes, while movements of 4 to 5mm were observed in the reference shaft. A simple computing model was developed to predict, based on suction changes, the maximum amount of ground heave. Relationship among suction. total stress, and volumetric strain was abtained in the laborstory. This relationship, used as inputs to the predictive model, enabled the computation of the maximum ground heave.

• Journal of the Korean GEO-environmental Society
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• v.8 no.5
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• pp.23-29
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• 2007

The performance of the improved soil against liquefaction depends upon the chemical density, and it has been decided on the basis of the unconfined compressive strength of the improved soil up to date. On the other hand, several authors have proposed that the stiffness degradation could be treated as the clue for the judgment of the possibility of liquefaction. In this study, therefore, the stiffness degradation of the improved soil was estimated as the resistance against liquefaction by using the strain controlled cyclic triaxial test equipment. Based on the test results, it is concluded that the chemically treated sand can resist against the liquefaction in aspect of the reduction in effective stress and in the stiffness. Furthermore, even in the case of low chemical density, such as 2% in this study, has enough liquefaction resistance when compared with the 5~6% which often used in practical design. Considering this fact, the design of chemical density based on the unconfined strength can lead the overestimation in chemical density, and chemical density can be reduced when considering the stiffness reduction shown in this study.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2000.11a
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• pp.153-162
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• 2000

A field study was performed to test effectiveness of a bloluminescent genetically engineered microorganism (GEM) for bioremediation process monitoring and control. The study employed Pseudomonas fluorescens HK44 that was the first strain approved for field application in the U.S. for bioremediation purposes. HK44 contains lux gene fused within a naphthalene degradative pathway, allowing this GEM to bioluminesce as it degrades naphthalene as well as substituted naphthalenes and other polycyclic aromatic hydrocarbons (PAHs) , Results showed that HK44 was maintained in both PAH-contarninated and uncontaminated soils even 660 days after inoculation. HK44 was able to produce bioluminescence in response to PAHs in soil. Although effectiveness of chemical remediation was not assessed due to heterogeneous distribution of contaminants, decreased concentration of naphthalene was shown in the soils, Taken together, HK44 was useful for in situ bioremediation process monitoring and control. This work is so far the only field release of a GEM for bioremediation purposes.

• The Plant Pathology Journal
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• v.32 no.2
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• pp.136-144
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• 2016

Pseudomonas fluorescens pc78 is an effective biocontrol agent for soil-borne fungal diseases. We previously constructed a P43-gfp tagged biocontrol bacteria P. fluorescens pc78-48 to investigate bacterial traits in natural ecosystem and the environmental risk of genetically modified biocontrol bacteria in tomato rhizosphere. Fluctuation of culturable bacteria profile, microbial community structure, and potential horizontal gene transfer was investigated over time after the bacteria treatment to the tomato rhizosphere. Tagged gene transfer to other organisms such as tomato plants and bacteria cultured on various media was examined by polymerase chain reaction, using gene specific primers. Transfer of chromosomally integrated P43-gfp from pc78 to other organisms was not apparent. Population and colony types of culturable bacteria were not significantly affected by the introduction of P. fluorescens pc78 or pc78-48 into tomato rhizosphere. Additionally, terminal restriction fragment length polymorphism profiles were investigated to estimate the influence on the microbial community structure in tomato rhizosphere between non-treated and pc78-48-treated samples. Interestingly, rhizosphere soil treated with strain pc78-48 exhibited a significantly different bacterial community structure compared to that of non-treated rhizosphere soil. Our results suggest that biocontrol bacteria treatment influences microbial community in tomato rhizosphere, while the chromosomally modified biocontrol bacteria may not pose any specific environmental risk in terms of gene transfer.

• Journal of Korea Soil Environment Society
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• v.4 no.2
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• pp.33-43
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• 1999

A strain (designated SK3l) which produces an excellent adsorption substance was isolated from soil samples and identified as Bacillus specied. The major adsorption substance (biosorbent SK3l) produced by Bacillus sp. SK31 was purified by ethanol precipitation and cetylpyridinium (CPC) precipitation. The adsorption charactics of zinc and lead ions on bioadsorbent SK3l were investigated. The equilibrium isotherms showed that bioadsorbent SK3l took up zinc and lead from aqueous solutions to the extent of about 52 mg/g and 112 mg/g. respectively. The culture conditions at the flask level of Bacillus sp. SK3l were investigated for the production of polysaccharide bioadsorbent, SK3l. The optimum pH and temperature for sorbent production were 7.5 and $30^{\circ}C$, respectively. The important carbon and nitrogen sources for sorbent formation were glucose and ammonium nitrate, respectively. In the optimized medium, sorbent production was improved three folds in comparison with the basal medium. In the jar fermenter, the highest sorbent production was obtained at 60 h cu1tivation time and the amount of biosorbent SK3l at that time was 9.2 g/$m\ell$.

• Korean Journal of Environmental Agriculture
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• v.20 no.1
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• pp.57-62
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• 2001

Twelve antagonistic strains against Fusarium wilt of watermelon-rootstock gourd were selected from 54 bacterial isolates which were isolated from the rhizosphere of crop plants growing in various locations. They showed strong inhibitory effects on growth of Fusarium osysporum f. sp. niveum, the causal agent of watermelon-rootstock gourd Fusarium wilt. Among these antagonists, the isolate YJ-3 was the most pronounced in growth-promoting ability for watermelon-rootstock gourd. The growth of watermelon-rootstock gourd in bed soil inoculated with YJ-3 was better by 46 and 13% than those in commercial bed soil alone and in bed soil inoculated with commercial microbial inoculant, respectively. The antagonistic plant growth-promoting rhizobacterium, strain No. YJ-3, was identified as Bacillus sp. on MIDI system. Furthermore, Bacillus sp. YJ-3 showed antifungal activity on growth against Alternaria cucumerina, Botrytis cinerea, Colletotrichum orbiculare, Didymella bryoniae, Rhizoctonia solani and Fusarium oxysporum.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.3
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• pp.87-93
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• 2008

The large scale shaker can be employed to measure linear and nonlinear shear moduli of soils in situ as a function of shear strain. The method involves applying dynamic loads on a surface foundation measuring the dynamic response of the soil mass beneath the foundation with embedded instrumentation. This paper focuses on the development of a framework of the inverse analysis for the interpretation of test data to estimate linear and nonlinear shear moduli of soils along with the necessity of the inverse analysis. The suggested framework is based on the nonlinear least squares but it uses two iterative loops to account for the nonlinear behavior of soil that sensors are not located. The validity of the suggested inversion framework is tested through a series of numerical parametric studies. An example use of the suggested inversion framework is also shown. Because the field condition may affect the accuracy of suggested method, it is important to conduct a preliminary inverse analysis to quantify the discrepancy between the estimated modulus and the baseline.

• Journal of the Korean Geosynthetics Society
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• v.9 no.3
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• pp.9-18
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• 2010

A series of model tests were performed to investigate the load transfer by soil arching in geosynthetic-reinforced and pile-supported(GRPS) embankment systems. In the model tests, model piles with isolated cap were inserted in the model container and geosynthetics was laid on the pile caps below sand fills. The settlement of soft ground was simulated by rubber form. The loads acting on pile caps and the tensile strain of geosynthetics were monitored by data logging system. At the given interval ratio of pile caps, the efficiency in GRPS embankment systems increased with increasing the height of embankment fills, then gradually converged at constant value. Also, at the given height of embankment fills, the efficiency decreased with increasing the pile spacing. The embankment loads transferred on pile cap by soil arching increased when the geosynthetics installed with piles. This illustrated that reinforcing with the geosynthetics have a good effect to restraint the movement of surrounding soft grounds. The load transfer in GRPS embankment systems was affected by the interval ratio, height of fills, properties of grounds and tensile stiffness and so on.

• Microbiology and Biotechnology Letters
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• v.20 no.3
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• pp.263-270
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• 1992

For the selection of powerful antagonistic bacterium for biological control of soil borne Eminia carotovora subsp. carotovora causing rot of vegetable, excellent strains (S4, S14, 565) were selected from 1,196 strains of bacteria which were isolated from rhizosphere in vegetable root rot-suppresive soil. Strains were identified to be Pseudomonas species with Api 20NE kit. Antagonistic substance was produced in 523 synthetic broth medium at pH 7~8 and $30^{\circ}C$ during 3 days culture. The substance was stable in the pH range of 6 to 9. When the basal medium was supplemented with mannitol and sorbitol as carbon source and calcium chloride as metal salt, the production of the inhibitory substance was increased. The inhibitory acitivity was increased by the addition of fertilizer in soil. The isolated strains were resistant to the agricultural chemical such as benomyl and fosethyl-Al-folpet, and the antibiotics such as penicillin and lincomycin. We had found that Pseudomonas sp. S14 strain had a single plasmid. After treated with acridin orange for curing, we confirmed the existence of antagonistic gene in the chromosomal DNA.

• Journal of the Korean Geosynthetics Society
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• v.10 no.1
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• pp.19-28
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• 2011

This paper presents the results of an investigation into the effect of cyclic freezing-thawing on the compressive strength characteristics of decomposed granite soils. A plane strain compression (PSC) tests were performed on a series of test specimens with different freezing-thawing cycles and fine contents to investigate the change in compressive strength under the process of freezing-thawing cycles. Also performed were scanning electron microscope (SEM) tests to investigate the change in structural rearrangement from a micro-scale point of view. The test results showed that the soil particles tend to conglomerate when subject to cycles of freezing and thawing, and that the soil with less fines exhibited decreased shear strength due to the cyclic freezing-thawing while the soils with a larger fine content showed the opposite trend.