• Journal of Microbiology and Biotechnology
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• v.3 no.2
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• pp.99-107
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• 1993

Two strains (No. 26 and 143) of bacteria which secrete both pectinase and raw-starch-digesting amylase simultaneously, were isolated from various domestic soil samples. The two bacteria were identified as Pasteurella ureae judging by their morphological and physiological characteristics. The optimal culture conditions for the production of raw-starch-digesting enzyme by the Pasteurella ureae 26 were using $NH_4NO_3$ as the nitrogen source at $37^{\circ}C$ with the pH of 7.5, and 15 of C/N ratio. Since the enzyme was produced only when raw or soluble starch was used as a carbon source, but not when glucose or other sugars was used, the enzyme was considered to be an inducible enzyme by starch. Thin layer chromatography of the hydrolyzed product of starch by the raw-starch-digesting enzyme of the strain No. 26 showed that glucose, maltose and other oligosaccharides were present in the hydrolyzates, and therefore the enzyme seemed to be ${\alpha}-amylase$. The enzyme had adsorbability onto raw com starch in the pH range of 3 to 9.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.10a
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• pp.389-396
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• 1999

In this study compressive and tensile load tests have been performed to investigate reinforcing effect and load transfer mechanism of small diameter piles installed in the foundation soil for the marine suspension bridge. Load tests were carried out on steel plate with diameters of 50cm, 100cm and 150cm varying loads starting from 39 tons up to 314 tons. Small diameter piles were proved to behavior like as friction piles and loads were not transmitted to the bottom of piles. From pull-out tests, the uplift capacity of small diameter piles was largely influenced by reinforcing materials compared to frictional resistance between piles and adjacent soils. The bearing capacity of small diameter piles appeared to be higher than the ultimate bearing capacity evaluated using static formulae. The load carrying capacity of small diameter piles was superior to the bored piles with a similar size. Thus, ultimate bearing capacity estimated from static formulae can provide conservative designs and thereby resulting in economic disadvantages. A further study to accumulate data regarding various soil conditions is recommended for an improved estimation of bearing capacity of piles with small diameter.

• Journal of the Korean Geotechnical Society
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• v.17 no.1
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• pp.119-129
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• 2001

연약지반 개량을 위한 시멘트의 사용은 깊은 심도의 점토 지반을 개량하는데 일반적으로 사용되는 기술이 되었다. 시멘트는 지반의 강도를 증가시키고 압축성을 감소시키는 역할을 한다. 시멘트-흙 혼합물의 강도 증가에는 여러 가지 요소가 있는데 이중 대표적인 것은 시멘트량, 흙의 종류, 함수비, 양생시간 등을 들 수 있다. 시멘트 첨가량이 적은 경우, 전단 강도증가는 기본적으로 시멘테이션 효과로 인한 점착력의 증가에 의한 입자들간의 마찰력으로부터 발생한다. 이러한 거동은 과압밀된 흙의 거동과 유사함을 볼 수 있다. 시멘트량이 많은 경우, 강도 증가의 주원인은 입자간의 물리적 결합에 기인하는데 이는 연약한 암석과 비슷한 거동을 한다. 시멘트 고화처리 흙의 응력-변형 거동을 분석하기 위해 한계상태 이론을 적용하였다. 그리고, 토립자간의 시멘테이션 효과를 반영하기 위해 새로운 한계상태 파라메타를 도입하였으며 시멘트 고화처리 점토의 거동을 분석하기 위한 새로운 한계상태 모델을 제시하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.4
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• pp.367-371
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• 2005

About 3,000 bacterial colonies with esterase activities were isolated from soil samples by enrichment culture and halo-size on Luria broth-tributyrin (LT) plates. The colonies were assayed for esterase activity in microtiter plates using enantiomerically pure (R)- and (S)-2-phenylbutyric acid resorufin ester (2PB-O-res) as substrates. Two enantioselective strains (JH2 and JH13) were selected by the ratio of initial rate of hydrolysis of enantiomerically pure (R)- and (S)-2-PB-O-res. When cell pellets were used, both strains showed high apparent enantioselectivity ($E_{app}>100$) for (R)-2PB-O-res and were identified as Exiguobacterium acetylicum. The JH13 strain showed high esterase activity on p-nitrophenyl acetate (pNPA), but showed low lipase activity on p-nitrophenyl palmitate (pNPP). The esterase was located in the soluble fraction of the cell extract. The crude intracellular enzyme preparation was stable at a pH range from 6.0 to 11.0.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.4
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• pp.314-318
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• 1984

China has made rapid strides in agricultural production during the past few years. Although the absence of any serious climatic disturbances is a contributing factor, implementation of the responsibility system to reward peasant families for their initiatives has been by far the most significant. This has resulted in increased specialization, a greater requirement for technical adivsory services, and a much higher demand for farm equipment and production inputs. The revamped system has brought with it some problems such as differences in income between the most progressive producers and their less successful neighbours and urban workers, a shortage of storage, transport and processing facilities, and a strain on the national treasury to continue to subsidize farm prices. Demand for fertilizers has been increasing at a fast rate, especially for nitrogen. This has led to a serious imbalance in nutrient use which must be corrected if the current momentum in improving crop yields is to be maintained. Following a decade of total stagnation during the Cultural Revolution, agricultural research and education facilities are being strengthened. Younger scientists, many with overseas training, are being placed in key positions. China is still a developing country, and much remains to be done; however, progress in the last decade, and especially in the past five years, has been remarkable.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.23-26
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• 2004

Methyl Tertiary-Butyl Ether is one of several fuel oxygenates added to gasoline to improve fuel combustion and reduce tile resulting concentration of hydrocarbon. Thus, MTBE transfer readily to groundwater from gasoline leaking from Underground Storage Tank. Therefor, there are significant risks and costs associated with the water contamination. MTBE is far more water soluble than gasoline hydrocarbon. The purpose of the this study is to test the ability of ground tire with facultative bacteria. Bacillus brevis, to sorb MTBE. The process is consisted both batch and column experiment to determine the sorption capacity. And Biofilm is observed by SEM in the column. Finally, it is clear that ground tire represent an attractive and relatively inexpensive sorption medium for a MTBE. The authors can surmise that to determine the economic cost of ground tire utilization, tile cost to sorb a given mass of contaminant by ground tire will have to be compared to currently accepted sorption media. and Bacillus brevis strain was eliminated on MTBE, too. The biobarrier that ground tire with bacteria, has potential for use in the remediation of MTBE-contaminated environments.

• Korean Journal of Veterinary Research
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• v.33 no.1
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• pp.61-69
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• 1993

In order to search for new antibiotics from anaerobic bacteria, a large number of samples from domestic soil were collected and processed by apropriate methods. A potential strain, Streptococcus sp. An-21-1, was found to produce antimicrobial compounds. The Results were as follows; 1. During fermentation, the bacteria grew rapidly up to 20hr, thereafter entered the death phase. The optimal temperature and pH for the bacterial growth were $37^{\circ}C$ and pH 7.0, respectively. 2. Antibiotics were purified from culture broth by solvent extraction, silica gel column chromatography and Sepadex L.H 20 column. 3. Physicochemical properties of Ap-1 and Ap-2 were similar ; Their melting points were between $234-237^{\circ}C$. Color reactions of ninhydrin, 2,7-dichlorofluorescein, 4-dimethylaminobenzaldehyde, Dragendroffs reagent and 20% $H_2SO_4$, were positive. Therefore, we assumed that these antibiotics have amine group, immine group, alkaloid, and lipid components. These were stable to heat. UV spectrophotometry showed two peaks at 210 nm and 260 nm. From above results, we assumed these antibiotics are belong to the peptide antibiotic family.

• Journal of Soil and Groundwater Environment
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• v.21 no.5
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• pp.16-24
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• 2016

A new strain of Pseudomonas sp. was isolated from mercury (Hg)-contaminated sites in Taiwan. This bacterium removed more than 80% of Hg present in the culture medium at 12 h incubation and was chosen for further analysis of the molecular mechanisms of Hg tolerance/removal abilities in this Pseudomonas sp. We used RNA-seq, one of the next-generation sequencing methods, to investigate the transcriptomic responses of the Pseudomonas sp. exposed to 60 mg/L of Hg²⁺. We de novo assembled 4,963 contigs, of which 10,533 up-regulated genes and 5,451 down-regulated genes were found to be regulated by Hg. The 40 genes most altered in expression levels were associated with tolerance to Hg stress and metabolism. Functional analysis showed that some Hg-tolerant genes were related to the mer operon, sulfate uptake and assimilation, the enzymatic antioxidant system, the HSP gene family, chaperones, and metal transporters. The transcriptome were analyzed further with Gene Ontology (GO) and Cluster of Orthologous Groups (COGs) of proteins and showed diverse biological functions and metabolic pathways under Hg stress.

• Microbiology and Biotechnology Letters
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• v.8 no.2
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• pp.87-92
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• 1980

These experiments were conducted to manage more safe the selected strains and improve their characteristics. The results obtained were as follows: Preservation on soil at the range of 0 to 5 C was suitable and there were no remarkable changes on their abilities to produce citric acid until 10 months preservation. The successive transfer of spore slightly stimulated the selected strain, M-315 to produce citric acid and the spore precultured on sporulation medium for 7-10 days was desirable as inoculum. Under UV-irradiation from the selected strains, 109 mutants whose morphological characteristics were changed were isolated. Among them, the mutant M-80-12 was shown 3.2％ increase on acid producing ability than that of its parent.

• Korean Journal of Microbiology
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• v.39 no.2
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• pp.102-107
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• 2003

With the enrichment culture technique, bacterial strains which degrade diesel oil were isolated from soil contaminated with diesel oil. One of the isolates named GENECO 1 showed the highest activity for emulsification of diesel oil as well as the highest growth rate. This strain, GENECO 1, was identified as a Pseudomonas sp. based on its biochemical, physiological characteristics and 16S rDNA sequences. The optimal cultural conditions for cell growth and oil emulsifying activity of its culture were as follow; $30^{\circ}C$ for temperature, 7.0 for pH. Diesel oil degradation was analysed by the gas chromatography. More than 95% of 1% treated diesel oil were converted into a form no longer extractable by mixed organic solvents after 96 hours incubation.