• YAKHAK HOEJI
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• v.36 no.4
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• pp.390-396
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• 1992

Streptomyces minoensis DMCJ-144 isolated from soil produces the ${\alpha}-amylase$ inhibitor. Optimum culture conditions for ${\alpha}-amylase$ inhibitor production of the strain were determined in this experiment. The optimum composition of the culture medium was studied by supplementing various carbon sources, nitrogen sources, vitamins, and metal salts to the basal medium containing 1% glucose, 0.1% asparagine, 0.005% $MgSO_4{\cdot}7H_2O$, 0.005% $K_2HPO_4$, 0.005% NaCl. Other culture conditions such as the culture temperature, initial pH of the medium, aeration, and culture time were also investigated. When the strain was cultured in a 100 ml flask containing 20 ml of 2% glucose, 0.5% beef extract, 0.0002% riboflavin, 0.0002% thiamine HCI, 0.01% $ZnCl_2$, 0.005% $MgSO_4{\cdot}7H_2O$, 0.005% $CuSO_4{\cdot}5H_2O$, 0.005% NaCl, pH 7.2, 180 rpm at $30^{\circ}C$, the maximum production of the ${\alpha}-amylase$ inhibitor was observed after 5 days of the cultivation.

• Journal of Radiation Industry
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• v.4 no.1
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• pp.65-71
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• 2010

Two chitinase producing strains CHI2 and CHI4 were isolated from soybean rhizosphere soil. Both the strains belonged to Lysobacter enzymogenes as indicated by 16S rDNA sequence analysis. Though strain CHI2 and CHI4 produced extracellular chitinase, they differ in their chitinolytic activity. CHI4 produced approximately three times the higher amounts of enzyme than that of CHI2 under specified conditions. CHI2 produced $535.67U\;l^{-1}$ of chitinase after 48 h incubation with a specific activity of $3.91U\;mg^{-1}$ of protein while strain CHI4 produced $1584.13U\;l^{-1}$ of chitinase with a specific activity of $10.88U\;mg^{-1}$ protein. SDS-PAGE analysis indicated that the molecular weight of chitinase enzyme was approximately 45 kDa. A faint band with a molecular weight of 55 kDa reveals the possibility for the presence of another kind of chitin binding protein. Mutant library was developed by exposing the isolates to gamma rays at their $LD_{99}$ value (0.23 kGy). Totally, 11 mutants of CHI2 and CHI4 are reported to have enhanced chitinase activity. Several leaky mutant clones with decreased enzyme activity and a defective mutant (CHI2-M16) with complete loss of chitinase activity were also identified. CHI4-M18, CHI4-M8 and CHI4-M29 showed 78.8, 41.5, and 31.9% increased chitinase activity over wild type CHI4.

• Journal of Species Research
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• v.8 no.4
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• pp.351-364
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• 2019

Here we describe indigenous prokaryotic species in Korea, a total of 42 bacterial strains affiliated to the class Alphaproteobacteria isolated from various environmental samples: fermented vinegar, sea water, beach sand, fresh water, salt flats, moss, algae, activated sludge, and soil. From the high 16S rRNA gene sequence similarity (>98.7%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to predefined bacterial species. There is no official report that these 42 species included in Alphaproteobacteria in Korea: 15 species of 6 genera in the order Rhodospirillales, 12 species of 10 genera in the order Rhizobiales, 10 species of 8 genera in the order Rhodobacterales, 4 species of 4 genera in the order Sphingomonadales and 1 species of 1 genus in the order Caulobacterales. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

• Mycobiology
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• v.47 no.3
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• pp.329-334
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• 2019

Streptomyces blastmyceticus strain 12-6 was isolated from a forest soil sample of Cheonan area on the basis of strong antifungal activities against plant pathogenic fungi. Butanol extracts of the cultural filtrates were active against C. acutatum, C. coccodes, C. gloeosporioides, F. oxysporum, and T. roseum. Active fractions were prepared by thin layer chromatography using silica gel plate; 12-6-2 ($R_f$ 0.36), 12-6-3 ($R_f$ 0.44). Scanning electron microscopy showed that the active fractions caused a change in surface texture of fungal spores from smooth surface to wrinkled surface. The lethal effect on the spores of the active fractions varied from 56% to 100%. It was shown that the spores of C. acutatum were more sensitive to the antifungal fractions than the spores of F. oxysporum. Fluorescence staining using TOTO-1 indicated that the antifungal fractions could make the spores more sensitive to the fluorescence dye. Thus, it was suggested that antifungal agents prepared in this study exhibited the antifungal activity by damaging the plasma membrane of both fungal spores and hyphae. Identification of antifungal agents in the active fraction using GC-MS analysis revealed the presence of cyclo-(Leu-Pro) and 9-octadecenamide as major components that have already been known as antifungal substances.

• Proceedings of the Korean Reliability Society Conference
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• 2005.06a
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• pp.69-73
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• 2005

The failure of geogrids used for soil reinforcement application can be defined as an excessive creep strain which causes the collapse of slopes and embankments. Accordingly, the lifetime is evaluated as a time to reach the excessive creep strain using two accelerated creep testing methods, time-temperature superposition(TTS) and stepped isothermal methods(SIM). TTS is a well-accepted acceleration method to evaluate creep behavior of polymeric materials, while SIM was developed in the last ten years mainly to shorten testing time and minimize the uncertainty associated with inherent variability of multi-specimen tests. The SIM test is usually performed using single rib of geogrids for temperature steps of $14^{\circ}C$ and a dwell time of 10,000 seconds. However, for multi-ribs of geogrids, the applicability of the SIM has not been well established. In this study, the creep behaviors are evaluated using multi-ribs of polyester geogrids using SIM and TTS creep procedures and the newly designed test equipment. Then the lifetime of geogrids are predicted by analyzing the failure times to reach the excessive creep strains through reliability analysis.

• Geomechanics and Engineering
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• v.17 no.5
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• pp.429-438
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• 2019

Microbially induced carbonate precipitation (MICP) is an innovative soil improvement approach utilizing metabolic activity of microbes to hydrolyze urea. In this paper, the shear response and the erodibility of MICP-treated sand under axial compression and submerged impinging jet were evaluated at a low confining stress range. Loose, poorly graded silica sand was used in testing. Specimens were cemented at low confining stresses until target shear wave velocities were achieved. Results indicated that the erodibility parameters of cemented specimens showed an increase in the critical shear stress by up to three orders of magnitude, while the erodibility coefficient decreased by up to four orders of magnitude. Such a trend was observed to be dependent on the level of cementation. The treated sand showed dilative behavior while the untreated sands showed contractive behavior. The shear modulus as a function of strain level, based on monitored shear wave velocity, indicated mineral debonding may commence at 0.05% axial strain. The peak strength was enhanced in terms of emerging cohesion parameter based on utilizing the Mohr-Coulomb failure criteria.

• Journal of Species Research
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• v.10 no.1
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• pp.12-22
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• 2021

During a comprehensive investigation of indigenous prokaryotic species in Korea, 25 and 10 bacterial strains assigned to the classes Alphaproteobacteria and Betaproteobacteria, respectively, were isolated from diverse environmental habitats, including soil, mud, tidal field, sea water, sand, rusted iron, and leaf. Based on their high 16S rRNA gene sequence similarities (>98.7%) and the formation of robust phylogenetic clades with type species, each strain was assigned to an independent and predefined bacterial species. Since there were no published or official reports regarding these 35 isolates in Korea, they - 25 species of 14 families in the 5 orders of Alphaproteobacteria and 10 species of 3 families in the two orders of Betaproteobacteria - have been reported as unrecorded species in Korea. In addition, Gram reaction, colony and cell morphology, basic biochemical characteristic, isolation source, and strain ID of each species are also described in the species description sections.

• Journal of Species Research
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• v.10 no.3
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• pp.191-200
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• 2021

To study the biodiversity of bacterial species, here we report indigenous prokaryotic species of Korea. A total of 23 bacterial strains affiliated to the class Alphaproteobacteria were isolated from various environmental sources including seaweeds, seawater, fresh water, wetland/marsh, tidal sediment, plant roots, sewage and soil. Considering higher than 98.8% 16S rRNA gene sequence similarities and formation of a well-defined phylogenetic clade with named species, it was confirmed that each strain belonged to the predefined bacterial species of the class Alphaproteobacteria. There is no official report of these 23 species in Korea; 20 species of 16 genera (Mameliella, Yangia, Paracoccus, Ruegeria, Loktanella, Phaeobacter, Dinoroseobacter, Tropicimonas, Lutimaribacter, Litoreibacter, Sulfitobacter, Roseivivax, Labrenzia, Hyphomonas, Maricaulis, Thalassospira) in the order Rhodobacterales and 3 species of a single genus (Brevundimonas) in the order Caulobacterales. Gram-staining, cell morphology, basic biochemical characteristics, isolation sources, optimum temperature, growth media, and strain IDs are detailed in the species description as well as Table 1.

• Geomechanics and Engineering
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• v.30 no.1
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• pp.27-44
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• 2022

Gravelly soil is a kind of special geotechnical material, which is widely used in the subgrade engineering of railway, highway and airport. Its mechanical properties are very complex, and will greatly influence the stability of subgrade engineering. To investigate the mechanical properties and failure mechanism of gravelly soils, this paper introduced and verified a new discrete element method (DEM) of gravelly soils in large scale direct shear test, which considers the actual shape and broken characteristics of gravels. Then, the stress and strain characteristics, particle interaction, particle contact force, crack development and energy conversion in gravelly soils during the shear process were analyzed using this method. Moreover, the effects of gravel content (GC) on the mechanical properties and failure characteristics were discussed. The results reveal that as GC increases, the shear stress becomes more fluctuating, the peak shear stress increases, the volumetric strain tends to dilate, the average particle contact force increases, the cumulative number of cracks increases, and the shear failure plane becomes coarser. Higher GC will change the friction angle with a trend of "stability", "increase", and "stability". Differently, it affects the cohesion with a law of "increase", "stability" and "increase".

• The Plant Pathology Journal
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• v.37 no.4
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• pp.389-395
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• 2021

Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces globisporus SP6C4 colonized plant tissues and was able to move to other tissues from the initially colonized ones. This strain has excellent antifungal and antibacterial activities and provides a suppressive effect upon various plant diseases. Here, we report the genome-wide analysis of antibiotic producing genes in S. globisporus SP6C4. A total of 15 secondary metabolite biosynthetic gene clusters were predicted using antiSMASH. We used the CRISPR/Cas9 mutagenesis system, and each biosynthetic gene was predicted via protein basic local alignment search tool (BLAST) and rapid annotation using subsystems technology (RAST) server. Three gene clusters were shown to exhibit antifungal or antibacterial activity, viz. cluster 16 (lasso peptide), cluster 17 (thiopeptide-lantipeptide), and cluster 20 (lantipeptide). The results of the current study showed that SP6C4 has a variety of antimicrobial activities, and this strain is beneficial in agriculture.