• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1606-1608
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• 2014

During an investigation of the fungal diversity of Korean soils, four Penicillium strains could not be assigned to any described species. The strains formed monoverticillate conidiophores with occasionally a divaricate branch. The conidia were smooth or finely rough-walled, globose to broadly ellipsoidal and $2.5-3.5{\times}2.0-3.0{\mu}m$ size. Their taxonomic novelty was determined using partial ${\beta}$-tubulin gene sequences and the ribosomal internal transcribed spacer region. The phylogenetic analysis showed that the isolates belonged to section Lanata-Divaricata and were most closely related to Penicillium raperi. Phenotypically, the strains differed from P. raperi in having longer and thicker stipes and thicker phialides. Strain KACC $47721^T$ from bamboo field soil was designated as the type strain of the new species, and the species was named Penicillium koreense sp. nov., as it was isolated from various regions in Korea.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.383-390
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• 2010

A newly isolated active producer of raw-starch-digesting amyloytic enzymes, Rhizopus microsporus var. chinensis CICIM-CU F0088, was screened and identified by morphological characteristics and molecular phylogenetic analyses. This fungus was isolated from the soil of Chinese glue pudding mill, and produced high levels of amylolytic activity under solid-state fermentation with supplementation of starch and wheat bran. Results of thin-layer chromatography showed there are two kinds of amyloytic enzymes formed by this strain, including one $\alpha$-amylase and two glucoamylases. It was found in the electron microscope experiments that the two glucoamylases can digest raw corn starch and have an optimal temperature of $70^{\circ}C$. These results signified that amyloytic enzymes secreted by strain Rhizopus microsporus var. chinensis CICIM-CU F0088 were types of thermostable amyloytic enzymes and able to digest raw corn starch.

• The Korean Journal of Mycology
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• v.17 no.3
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• pp.149-153
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• 1989

One strain of Aspergillus niger K-25 producing an acid-stable ${\alpha}-amylase$ was isolated from the soil. The optimum culture conditions were investigated. The production of the acid-stable ${\alpha}-amylase$ was enhanced when the strain was incubated in a medium containing soluble starch 3.5%, peptone 2%, $KH_2PO_4$ 0.5%, $MaSO_4{\cdot}7H_2O$ 0.25% and $FeCI_3$ 1.0% at pH 3 for 7 days. However, higher activity of acid-stable ${\alpha}-amylase$ was demonstrated on wheat bran culture. Amylase production was doubled when A. niger K-25 was incubated on the wheat bran supplemented with fumaric acid buffer (pH 3).

• Korean Journal of Microbiology
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• v.30 no.6
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• pp.501-506
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• 1992

An alkalophilic Vibrio sp. RH530 showing high proteolytic activity was isolated form soil samples by enrichment culture. The activity staining using gelatin SDS- polyacrylamide gel electrophoresis (PAGE ) revealed that the strain produced an alkaline major protease (Apr B) with a size of 27 kDa, and at least six minor proteases. The apparent sizes of four of the minor proteases were approximately 45, 28, 22 and 19 kDa. Apr B and five of the minor proteases were inhibited by serine protease inhibitors including PMSF and DFP, suggesting that they are serine proteases. One of the minor proteases was inhibited by metalloprotease inhibitors, not by serine protease inhibitors, indicating it to be a metalloprotease. Furthermore, the activities of Apr B and Prt 3 were not inhibited by SDS in the reaction mixture. The production of Apr B and some of the minor proteases was specifically affected by culture temperature (30 to 37.deg.C) and pH (7 to 10). The production of Apr B. Prt 2, Prt 5 and Prt 6 was mainly affected by culture temperature, while Prt 4 by culture pH. Prt 1 and Prt 3 were not affected by neither of these factors.

• Korean Journal of Agricultural Science
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• v.2 no.2
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• pp.463-468
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• 1975

1) 224 strains were isolated from the accumulated soil and sewage samples flowing the waste of alcohol distillation, and among of them 2 strains of yeast were selected on the basis of their superior growth in the medium containing alcohol waste by shaking culture. 2) Morphological and physiological characteristics of the selected strains were investigated, and strain-73, strain-124 were identified Candida ciferrii, Candida brumptii by the manual of LODDER, respectively.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.71-77
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• 2008

Microstructural changes may arise due to the particle vanishing, fluid diffusion, heating, etc. This study focuses on the changes in small-strain shear stiffness in k0 loading produced by local straining in particular system made of sand-salt mixtures. Local strains were induced by dissolution of salt particles. Experiments were carried out in a conventional oedometer cell equipped with bender elements. Axial displacement and shear wave signals are recorded at each loading stage and during saturation process. Experimental data showed that microstructural changes due to particle vanishing were clearly captured by using shear wave measurement. Saturation of sand-salt mixture at a larger axial stress did not always create a more condense soil at the end of loading stage. Sand-salt mixture is useful for laboratory test on controlled artificial specimen.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.1159-1166
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• 2005

In the present study, a series of laboratory tests with sands of different silt contents, are conducted and methods to assess non-linear behaviors based on in-situ test results are proposed. Modified hyperbolic stress-strain model is used to analyze non-linearity of silty sands in terms of non-linear degradation parameters f and g as a function of silt contents and relative density $D_R$. Stress-strain relationship results were obtained from a series of triaxial tests on sands containing different amounts of silt. Initial shear modulus which was applied to normalize modulus degradation of silty sands were determined based on the resonant column test results. From the laboratory test results, it was observed that, as the relative density increases, values of f decrease and those of g increase. Cone resistance $q_c$ for silty soil condition used in the triaxial tests were estimated based on the cavity expansion analysis. A suggestion to make an estimation of degradation parameters f and g as a function of fine contents is addressed in terms of cone resistance $q_c$ .

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.320-327
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• 2005

Liquefaction characteristics of saturated sand under various dynamic loadings such as sinusoidal, wedge, increasing wedge and real earthquake loading are investigated focusing on the excess pore water pressure build up instead of liquefaction resistance strength in this paper. There are large differences between two types of earthquake loading - impact and vibration in liquefaction characteristics. The angle of phase change line of sinusoidal loading is very close to the vibration type, whereas the cumulative deviator stress and cumulative plastic strain are larger than two types of real earthquake loadings. On the other hand, the liquefaction characteristics of increasing wedge loadings are located in the range between vibration and impact earthquake loadings. It is concluded that the sinusoidal loading overestimates the resistance of soil under real earthquake loading. Based on results obtained, the increasing wedge loading can reflect the liquefaction behavior under real earthquake loadings more efficiently than sinusoidal loading based on equivalent uniform stress concept.

• Journal of Mushroom
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• v.13 no.4
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• pp.275-281
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• 2015

An auxin-producing bacteria (A-1) was isolated from soils of Oyster mushroom farmhouse in Daejeon city, South Korea. The strain A-1 was classified as a novel strain of Ochrobactrum anthropi based on a chemotaxanomic and phylogenetic analyses. The isolate was confirmed to produce indole-3-acetic acid (IAA), one of auxin hormones, by TLC and HPLC analyses. The maximum concentration of IAA, $5.6mg\;L^{-1}$ was detected from the culture broth of O. anthropi A-1 incubated for 24 h at $35^{\circ}C$ in R2A broth containing 0.1% L-tryptophan. To investigate the growth-promoting effects to the crops, the culture broth of O. anthropi A-1 was inoculated to water cultures and seed pots of mung bean as well as lettuce. In consequence, the adventitious root induction and root growth of mung bean and lettuce were 2.7 and 1.4 times higher than those of the non-inoculated, respectively.

• Journal of the Korean Geotechnical Society
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• v.20 no.7
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• pp.183-196
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• 2004

Sand Compaction Pile (SCP) is a soft-ground improvement technique used for not only accelerating consolidation but also increasing bearing capacity of soils. In this study, laboratory tests and 3-D finite element analysis were peformed to investigate the characteristics of load transfer in SCP with an emphasis on free-strain behavior of piles with low replacement ratios in the range of 30 to $50\%$. Through these focused tests and numerical analyses, we proposed a simplified method to analyze the load transfer characteristics of SCP in soft ground. Moreover, it was shown that estimated normal stresses in SCP using the proposed method were in a reasonable agreement with actual values.