• Title/Summary/Keyword: soil organic carbon

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Production of Bioflocculant by Agrobacterium sp. KF-67 (Agrobacterium sp. KF-67에 의한 미생물 응집제 생산)

  • 정준영;김교창;도대홍
    • The Korean Journal of Food And Nutrition
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    • v.10 no.3
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    • pp.295-301
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    • 1997
  • Among 120 microorganisms isolated from soil, KF-67 was the best producer of flocculant and was examined for flocculating ability in the kaolin clay and CaCl2 suspension. KF-67 was identified to be a species belong to the genus Agrobacterium sp. The influence of components of the culture medium for flocculant production by Agrobacterium sp. KF-67 was studied. The favorable carbon and inorganic nitrogen source for production of the flocculant were glucose and NH4NO3 and their addition concentrations were 2% and 0.1%, respectively. Addition of the organic nitrogen such as yeast extract, peptone and inorganic salt such as CaCO3 significantly increased the production of flocculant. These result indicated that the production of flocculant by Agrobacterium sp. was significantly affected by both organic nitrogen and inorganic salt. The components of the optimum culture medium were 2% glucose, 0.1% NH4NO3, 0.01% yeast extract, 0.01% peptone, 0.04% CaCO3, 0.03% NaCl in initial pH 7.5 when cultured with rotary shaker controlled at 3$0^{\circ}C$ and 120 rpm. Under the optimum culture medium, flocculant production was highly improved about 76% than that isolation medium.

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Efficiency of Poultry Manure Biochar for Stabilization of Metals in Contaminated Soil (계분 바이오차를 이용한 토양 중금속 안정화 효율 평가)

  • Lim, Jung Eun;Lee, Sang Soo;Ok, Yong Sik
    • Journal of Applied Biological Chemistry
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    • v.58 no.1
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    • pp.39-50
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    • 2015
  • Stabilization of heavy metals such as Pb, Cd, Zn, and Cu was evaluated in contaminated soil treated with poultry manure (PM) as well as its biochars pyrolyzed at $300^{\circ}C$ (PBC300) and $700^{\circ}C$ (PBC700) at the application rates of 2.5, 5.0, and 10.0 wt% along with the control, prior to 21-days incubation. After incubation, soil pH was increased from 6.94 (control) to 7.51, 7.24, and 7.88 in soils treated with PM 10 wt%, PBC300 10 wt%, and PBC700 10 wt% treatments, respectively, mainly due to alkalinity of treatments. In the soil treated with PM, the concentrations of the toxicity characteristic leaching procedure (TCLP)-extractable Pb, Cd, Zn, and Cu were increased by up to 408, 77, 24, and 955%, respectively, compared to the control. These increases may possibly be associated with an increased dissolved organic carbon concentration by the PM addition. However, in the soil treated with PBC700, TCLP-extractable Pb, Cd, Zn, and Cu concentrations were reduced by up to 23, 38, 52, and 36%, respectively, compared to the control. Thermodynamic modelling using the visual MINTEQ was done to predict the precipitations of $Pb(OH)_2$, $Cu(OH)_2$ and P-containing minerals, such as chloropyromorphite [$Pb_5(PO_4)_3Cl$] and hydroxypyromorphite [$Pb_5(PO_4)_3OH$], in the PBC700 10 wt% treated soil. The SEM-elemental dot mapping analysis further confirmed the presence of Pb-phosphate species via dot mapping of PBC700 treated soil. These results indicate that the reduction of Pb concentration in the PBC700 treated soil is related to the formations of chloropyromorphite and hydroxypyromorphite which have very low solubility.

Verification of International Trends and Applicability in the Republic of Korea for a Greenhouse Gas Inventory in the Grassland Biomass Sector (초지 바이오매스 부문 온실가스 인벤토리 구축을 위한 국제 동향과 국내 적용 가능성 평가)

  • Sle-gee Lee;Jeong-Gwan Lee;Hyun-Jun Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.4
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    • pp.257-267
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    • 2023
  • The grassland section of the greenhouse gas inventory has limitations due to a lack of review and verification of biomass compared to organic carbon in soil while grassland is considered one of the carbon storages in terrestrial ecosystems. Considering the situation at internal and external where the calculation of greenhouse gas inventory is being upgraded to a method with higher scientific accuracy, research on standards and methods for calculating carbon accumulation of grassland biomass is required. The purpose of this study was to identify international trends in the calculation method of the grassland biomass sector that meets the Tier 2 method and to conduct a review of variables applicable to the Republic of Korea. Identify the estimation methods and access levels for grassland biomass through the National Inventory Report in the United Nations Framework Convention on Climate Change and type the main implications derived from overseas cases. And, a field survey was conducted on 28 grasslands in the Republic of Korea to analyse the applicability of major issues. Four major international issues regarding grassland biomass were identified. 1) country-specific coefficients by land use; 2) calculations on woody plants; 3) loss and recovery due to wildfire; 4) amount of change by human activities. As a result of field surveys and analysis of activity data available domestically, it was found that there was a significant difference in the amount of carbon in biomass according to use type classification and climate zone-soil type classification. Therefore, in order to create an inventory of grassland biomass at the Tier 2 level, a policy and institutional system for making activity data should develop country-specific coefficients for climate zones and soil types.

Physical and Chemical Properties of Soils in Quercus acutissima, Q. mongolica, Q. serrata, and Q. variabilis stands (상수리나무, 신갈나무, 졸참나무, 굴참나무 임분의 토양 물리·화학적 특성)

  • Sang Tae, Lee;Sang Hoon, Chung;Choonsig, Kim
    • Journal of Korean Society of Forest Science
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    • v.111 no.4
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    • pp.530-537
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    • 2022
  • This study was conducted to compare the physical and chemical properties of soils in Quercus acutissima, Q. mongolica, Q. serrata, and Q. variabilis stands. A total of 423 plots (Q. acutissima 72 plots, Q. mongolica 150 plots, Q. serrata 97 plots, and Q. variabilis: 104 plots) were examined to determine the soil properties of A and B horizons throughout the country. The physical and chemical properties of soils were significantly different among the four different Quercus spp. stands. The sand content in both horizons was significantly higher in Q. acutissima stands than in the other three oak stands, whereas the clay content was lowest among the four stands. The soils in Q. mongolica and Q. serrata stands were more acidified than those in Q. acutissima and Q. variabilis stands. The concentrations of organic carbon and total nitrogen in both soil depths were significantly higher in Q. mongolica stands than in Q. serrata, Q. variabilis, and Q. acutissima stands. The content of available phosphorus was significantly higher in Q. mongolica and Q. serrata stands than in Q. acutissima and Q. variabilis stands; whereas the contents of exchangeable potassium, calcium, and magnesium were lower in Q. mongolica and Q. serrata stands than in Q. acutissima and Q. variabilis stands. The cation exchange capacity was highest in Q. mongolica stands, followed by that in Q. serrata, Q. variabilis, and Q. acutissima stands. These data indicate the need to design a nutrient management plan to enhance the low soil pH and exchangeable cation in Q. mongolica and Q. serrata stands.

Growth and Nutrient Dynamics of Planted Tree Species Following Fertilization in a Fire-Disturbed Urban Forest (도시 숲 산불피해지의 시비에 따른 식재 수목의 생장 및 양분 동태)

  • Choonsig Kim;Gyeongwon Baek
    • Journal of Korean Society of Forest Science
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    • v.113 no.2
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    • pp.143-152
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    • 2024
  • This study was conducted to evaluate the growth and nutrient dynamics in response to fertilization of four tree species (LT: Liriodendron tulipifera L.; PY: Prunus yedoensis Matsumura; QA: Quercus acutissima Ca rruth; a nd PT: Pinus thunbergii Parl.) planted in a fire-disturbed urban forest in Bongdaesan (Mt.), Ulsan Metropolitan Area, South Korea. The trees were planted in 2009, and compound fertilizers (N6P4K1) were applied in April 2013 and March 2014. Tree growth, soil, and foliage nutrients were examined from March 2013 to October 2016. The regression coefficients for the increment of the diameter at breast height (DBH) significantly differed between the fertilized and unfertilized plots, suggesting the significant effects of fertilization. By contrast, fertilization did not affect the coefficients for height increments. Regarding soil nutrient contents, organic carbon and total nitrogen concentrations were lower in the fertilized plots than in the unfertilized plots, whereas available phosphorus, exchangeable calcium, and magnesium concentrations were higher in the fertilized plots than in the unfertilized plots. In foliage, nitrogen and phosphorus concentrations were higher in the fertilized plots than in the unfertilized plots, whereas potassium, calcium, and magnesium concentrations were not affected by fertilization. Nutrient concentration of foliage among the tree species were higher in LT and PY than in QA and PT. These results suggest that fertilizers may be used to enhance soil fertility and the growth and nutrient status of tree species planted in a fire-disturbed urban forest.

Short-Term Effect of Elevated Temperature on the Abundance and Diversity of Bacterial and Archaeal amoA Genes in Antarctic Soils

  • Han, Jiwon;Jung, Jaejoon;Park, Minsuk;Hyun, Seunghun;Park, Woojun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.9
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    • pp.1187-1196
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    • 2013
  • Global warming will have far-reaching effects on our ecosystem. However, its effects on Antarctic soils have been poorly explored. To assess the effects of warming on microbial abundance and community composition, we sampled Antarctic soils from the King George Island in the Antarctic Peninsula and incubated these soils at elevated temperatures of $5^{\circ}C$ and $8^{\circ}C$ for 14 days. The reduction in total organic carbon and increase in soil respiration were attributed to the increased proliferation of Bacteria, Fungi, and Archaea. Interestingly, bacterial ammonia monooxygenase (amoA) genes were predominant over archaeal amoA, unlike in many other environments reported previously. Phylogenetic analyses of bacterial and archaeal amoA communities via clone libraries revealed that the diversity of amoA genes in Antarctic ammonia-oxidizing prokaryotic communities were temperature-insensitive. Interestingly, our data also showed that the amoA of Antarctic ammonia-oxidizing bacteria (AOB) communities differed from previously described amoA sequences of cultured isolates and clone library sequences, suggesting the presence of novel Antarctic-specific AOB communities. Denitrification-related genes were significantly reduced under warming conditions, whereas the abundance of amoA and nifH increased. Barcoded pyrosequencing of the bacterial 16S rRNA gene revealed that Proteobacteria, Acidobacteria, and Actinobacteria were the major phyla in Antarctic soils and the effect of short-term warming on the bacterial community was not apparent.

Single-particle Characterization of Aerosol Samples Collected at an Underground Shopping Area (단일입자분석법을 이용한 지하상가에서 채취한 실내입자의 특성분석)

  • Kang, Sun-Ei;Hwang, Hee-Jin;Park, Yu-Myung;Kang, Su-Jin;Kim, Hye-Kyung;Ro, Chul-Un
    • Journal of Korean Society for Atmospheric Environment
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    • v.24 no.5
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    • pp.594-603
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    • 2008
  • A single particle analytical technique, named low-Z particle electron probe X-ray microanalysis, was applied to characterize four samples collected at an underground shopping area connected to Dongdeamun subway station, in January and May 2006. Based on the analysis of their chemical compositions of the samples, many distinctive particle types are identified and the major chemical species are observed to be soil-derived particles, iron-containing particles. sulfates. nitrates, and carbonaceous particles. which are encountered both in coarse and fine fractions. Carbonaceous particles exist in carbon-rich and organic. Soil derived particles such as aluminosilicates, AlSi/C, $CaCO_3\;and\;SiO_2$ are more frequently encountered in spring samples than winter samples. Nitrate- and sulfate-con taming particles are more frequently encountered in winter samples, and those nitrate- and sulfate-containing particles mostly exist in the chemical forms of $Ca(CO_3,\;NO_3),\;Ca(NO_3,\;SO_4),\;(Na,\;Mg)NO_3\;and\;(Mg,\;Na)(NO_3,\;SO_4)$. Fe-containing particles which came from nearby subway platform are in the range of about 10% relative abundances for all the samples. It is observed that nitrate- and sulfate-containing particles and carbonaceous particles are much more frequently encountered in indoor aerosol samples than in outdoor aerosols, implying that $NO_x,\;SO_x$, and VOCs at the underground shopping area were more partitioned into aerosol phase.

Steelers' Dust-catalyzed Oxidative Treatment of Landfill Leachate (제강분진을 이용한 매립지 침출수의 산화처리)

  • 강정우;김성용;장윤영;배범한;장윤석
    • Journal of Soil and Groundwater Environment
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    • v.7 no.1
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    • pp.25-31
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    • 2002
  • This study attempts to elucidate the possibility of treating landfill leachate by steelers' dust/${H_2}{O_2}$ system in a well-mixed batch reactor. Experiments were conducted to investigate the effects of operating variables such as pH, dust and ${H_2}{O_2}$ dosages and ${H_2}{O_2}$ injection method on the treatment efficiency, Contaminant concentrations were identified by TOC (Total Organic Carbon) analyzer. Experimental observations showed the possibility of steelers' dust as a catalytic reagent in the ${H_2}{O_2}$/dust system. The batch experiments showed that the optimal $H_2O$$_2$ and dust dosage, 20g/L dust. 2,000mg/L ${H_2}{O_2}$ removed about 75% of initial TOC concentration (250mg/1) within 60 min. And the TOC removal in the ${H_2}{O_2}$/dust system effectively proceeded only within a limited pH range of 3-4. The stealers' dust-catalyzed oxidative treatment of landfill leachate was more efficient compared with the $FeSO_2$catalyzed system (Fenton oxidation) for the removal rate and the cost.

Recovery of Ammonium Nitrogen and Phosphate from the Piggery Wastewater as Struvite and Its Assessment for the Reduction of Water Pollution Through the Field Test

  • Daeik Kim;Sun Jin Hwang;Su Ho Bae;Keon Sang Ryoo
    • Korean Journal of Environmental Agriculture
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    • v.42 no.2
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    • pp.83-92
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    • 2023
  • Excess N and P from the livestock manure applied to farmlands, have entered the water systems and poses a serious threat to the natural environment. Consequently, there has been recent awareness towards the management of livestock manure and its related fields. In this study, piggery wastewater was collected from a piggery in Pohang city, Korea. At 800℃, thermal decomposition of a natural stone, magnesite (MgCO3), yielded powered MgO with particle sizes ranging between 10 to 100 ㎛. Furthermore, NH4+-N and PO43--P were recovered as struvite precipitates from the piggery wastewater, by adjusting the pH with MgO and H3PO4. At pH 10, the recovery efficiencies of NH4+-N and PO43--P were found to be 86.1% and 94.1%, respectively. Using an X-ray Diffractometer (XRD), the struvite in the precipitate was confirmed to be consistent with standard pure struvite. Further, the purity of the struvite precipitate was analyzed using an energy dispersive X-ray (EDX) and thermal gravimetry-differential thermal analysis (TG-DTA), and found to be between 79.2% and 93.0%. Additionally, struvite-containing piggery wastewater and sawdust were mixed in a weight ratio of 2.5:1 and processed into a mature compost. The newly manufactured compost passed all quality standards required for first-class graded livestock composts. Moreover, this compost was sprayed directly onto the soil at the test site, and various parameters of the soil's effluent, such as total organic carbon (TOC), total nitrogen (T-N), total phosphorus (T-P), and dissolved oxygen (DO), were analyzed and measured. Based on these results, it is determined that the newly manufactured compost can more significantly reduce water pollution than commercial compost.

Application of LCA Methodology on Lettuce Cropping Systems in Protected Cultivation (시설재배 상추에 대한 전과정평가 (LCA) 방법론 적용)

  • Ryu, Jong-Hee;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.705-715
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    • 2010
  • The adoption of carbon foot print system is being activated mostly in the developed countries as one of the long-term response towards tightened up regulations and standards on carbon emission in the agricultural sector. The Korean Ministry of Environment excluded the primary agricultural products from the carbon foot print system due to lack of LCI (life cycle inventory) database in agriculture. Therefore, the research on and establishment of LCI database in the agriculture for adoption of carbon foot print system is urgent. Development of LCA (life cycle assessment) methodology for application of LCA to agricultural environment in Korea is also very important. Application of LCA methodology to agricultural environment in Korea is an early stage. Therefore, this study was carried out to find out the effect of lettuce cultivation on agricultural environment by establishing LCA methodology. Data collection of agricultural input and output for establishing LCI was carried out by collecting statistical data and documents on income from agro and livestock products prepared by RDA. LCA methodology for agriculture was reviewed by investigating LCA methodology and LCA applications of foreign countries. Results based on 1 kg of lettuce production showed that inputs including N, P, organic fertilizers, compound fertilizers and crop protectants were the main sources of major emission factor during lettuce cropping process. The amount of inputs considering the amount of active ingredients was required to estimate the actual quantity of the inputs used. Major emissions due to agricultural activities were $N_2O$ (emission to air) and ${NO_3}^-$/${PO_4}^-$ (emission to water) from fertilizers, organic compounds from pesticides and air pollutants from fossil fuel combustion in using agricultural machines. The softwares for LCIA (life cycle impact assessment) and LCA used in Korea are 'PASS' and 'TOTAL' which have been developed by the Ministry of Knowledge Economy and the Ministry of Environment. However, the models used for the softwares are the ones developed in foreign countries. In the future, development of models and optimization of factors for characterization, normalization and weighting suitable to Korean agricultural environment need to be done for more precise LCA analysis in the agricultural area.