• Korean Journal of Plant Tissue Culture
• /
• v.27 no.2
• /
• pp.125-131
• /
• 2000

Protoplasts of Arabidopsis thaliana were easily isolated from the shoot-forming (SF) suspension-cultured cell clusters with 4 hours-shaking condition (40 rpm) on CPD enzyme solution containing 1% cellulase R-10, 0.25% pectolyase Y-23 and 0.5% driselase. Protoplasts were cultured on liquid KAO medium supplemented with 1 mg/L 2,4-D, 0.5 mg/L kinetin, 200 mg/L spermidine and 68 g/L glucose. Also, protoplasts were cultured on 0.2 $\mu$M membrane filter placed onto CP solid medium containing the suspension cells as feeder cells in the dark at $25^{\circ}C$ for 4 weeks. Protoplast-derived-SF calli were cultured on MS medium containing 0.05 mg/L IAA, 7 mg/L 2 ip and 30 g/L sucrose under the continuous illumination for four weeks. The frequency of shoot formation was about 60%. The regenerants were transferred into potting soil to grow mature plants. The regenerants formed the silques with seeds after 8 weeks of cultures.

• Journal of Korean Society on Water Environment
• /
• v.30 no.5
• /
• pp.549-558
• /
• 2014

Occurrence frequency of flood and drought tends to increase in last a few decades, leading to social and economic damage since the abnormality of climate changes is one of the causes for hydrologic facilities by exceedance its designed tolerance. Soil and Water Assessment Tool (SWAT) model was used in the study to estimate temporal variance of groundwater recharge and baseflow. It was limited to consider recession curve coefficients in SWAT model calibration process, thus the recession curve coefficient was estimated by the Baseflow Filter Program (BFLOW) before SWAT model calibration. Precipitation data were estimated for 2014 to 2100 using three models which are GFDL-ESM2G, IPSL-CM5A-LR, and MIROC-ESM with Representative Concentration Pathways (RCP) scenario. SWAT model was calibrated for the Soyang watershed with NSE of 0.83, and $R^2$ of 0.89. The percentage to precipitation of groundwater recharge and baseflow were 27.6% and 17.1% respectively in 2009. Streamflow, groundwater recharge, and baseflow were estimated to be increased with the estimated precipitation data. GFDL-ESM2g model provided the most large precipitation data in the 2025s, and IPSL-CM5A-LR provided the most large precipitation data in the 2055s and 2085s. Overall, groundwater recharge and baseflow displayed similar trend to the estimated precipitation data.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.60 no.1
• /
• pp.31-36
• /
• 2018

A rainwater harvesting device was developed for runoff flow harvesting in a small stream or channel and its performance was evaluated in small fields. The rainwater harvesting device has slits on its side of cylindrical volume in 15 cm diameter and 70 cm length, which is designed to increase its water flow harvesting capacity. The maximum collectable water quantity was about 0.0022 ton/sec (130 L/min). Rainwater harvesting device were installed in two locations (P1, P2). P1 is a point for rainfall runoff flow harvesting. P2 is a point for ordinary flow harvesting. During this study, total rainfall depth was 334.5 mm. Runoff of 1,722 ton and 7,984 ton occurred in P1 and P2, and 273 ton and 125 ton were collected by this rainwater harvesting device. Harvesting efficiency was calculated as 15.85 % and 1.57 % in P1 and P2. Clogging of screen filter media in the cylinder due to soil and suspended solids has lowered the harvesting efficiency. However, it was possible to harvest 30 ton/month of rainwater harvesting and it is expected that it will help to solve short-term water shortage.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.62 no.4
• /
• pp.99-110
• /
• 2020

The objective of this study is to analyze the trend of changes in the water circulation rates under climate change by adopting the concept of WCR defined by the Ministry of Environment. With the need for sound water circulation recovery, the MOE proposed the idea of WCR as (1-direct flow/precipitation). The guideline for calculating WCR suggests the SCS method, which is only suitable for short term rainfall events. However, climate change, which affects WCR significantly, is a global phenomenon and happens gradually over a long period. Therefore, long-term trends in WCRs should also be considered when analyzing changes in WCR due to climate change. RCP (Representative Concentration Pathway) 4.5 and 8.5 scenarios were used to simulate future runoff. SWAT (Soil and Water Assessment Tool) was run under the future daily data from GCMs (General Circulation Models) after the calibration. In 2085s, monthly WCR decreased by 4.2-9.9% and 3.3-8.7% in April and October. However, the WCR in the winter increased as the precipitation during the winter decreased compared to the baseline. In the aspect of yearly WCR, the value showed a decrease in most GCMs in the mid-long future. In particular, in the case of the RCP 8.5 scenario, the WCR reduced 2-3 times rapidly than the RCP 4.5 scenario. The WCR of 2055s did not significantly differ from the 2025s, but the value declined by 0.6-2.8% at 2085s.

• Geotechnical Engineering
• /
• v.2 no.3
• /
• pp.51-66
• /
• 1986

The design of fabric reinforced retaining wall structure was discussed in this article. It was confirmed that the reinforced retaining earth wall which was designed by new theoretical formulae developed this time was stable structurally and economically. The plastic fabric filter which was placed in layers behind the facing element reduced the lateral earth pressure on the wall elements in comparison with a conventional retaining earth walls. The reinforcing characteristics of earth wall was governed by the spacing of fabric layers, effective length of fabrics, particle distribution and compaction, and thus it is essential that, in the construction field, the reinforcing strips should be selected in order to develop the maximum friction forces bet.eon soil and fabric filters. The maximum tensile stress developed from the reinforcing strips was appeared at a little far distance from the back of skin element and it was not well agreed with the Rankine's theory but distributed well as a symmetrical shape against the point of the maximum tensile stress. The total length of the different layers should be sufficient so that the tension in the fabric strip could be transferred to the backfill material. Also the total stability of reinforced earth wall should be checked with respect to a failure surface which extended blond the different lathers.

• Journal of the Korea Institute of Building Construction
• /
• v.18 no.1
• /
• pp.9-15
• /
• 2018

Radon gas, which is present on the earth, is a primary carcinogen released from rocks, soil, building materials, etc., and exists as a unique gas phase. In order to solve the risk of radon gas, we evaluated the basic performance which can be used as indoor finishing materials in addition to the radon gas reduction properties of the matrix using anthracite. An anthracite used as a conventional filter material was used to produce a matrix, and a test was conducted to replace the gypsum board, which is one of the building materials used in the existing room. As the anthracite replacement ratio increases, flexural failure load strength increases and thermal conductivity tends to decrease. Depending on the thickness of the board, the reduction performance of radon gas shows a slight difference.

• Applied Biological Chemistry
• /
• v.11
• /
• pp.109-117
• /
• 1969

Out of some 400 strains of Microorganisms, cellulase forming organisms was isolated from night soil during the course of screening tests. Two strains, Ku-3371 and Ku-4383 were found capable of producing cellulase in the shaking culture. General properties of the crude enzyme were as the following results. 1. The optimum pH values on CMC-saccharifying, CMC-liquefying and filter paper disintegrating activities were 4.0 to 5.5. 2. The stable pH range was within 3.5 to 6.5, 3. The optimum temperature was $40-45^{\circ}C$, the thermal stability was below $50^{\circ}C$ except on paper disintegrating activity and completely inactivated at $70^{\circ}C$. 4. Dialyzed crude enzyme was activated by $Mn^{2+}\;and\;Co^{2+}$ repectively but $Hg^{2+}$ was strong inhibitor.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.15 no.2
• /
• pp.80-86
• /
• 1995

High salinity common in reclaimed soil can reduce germination of crop seeds and aftermath its establishment and production. The experiment was done to measure germination and radicle elongation of berseem, subterranean, white clover and Italian ryegrass as Control under different temperature and NaCl concentrations. Onehundred seeds of berseem (Trifolium ulexandrinum cv. Bigbee). suberranean (T. suhterruneum, cv. Nungarin), white clover (T. repens, cv. Regal) and Italian ryegrass (Lolium mulriflorum, cv. Terafloum) were placed in petri dishes with a sheet of filter paper replaced every two days, and then exposed to 0, 12.5, 25. 50, 100 and 200mh4 of sodium chloride in darkened growth chambers controlled with 10, 15 and $20^{\circ}C$. Percent germination and radicle elongation were measured. Beneem clover showed greatest daily percent germination among the cloven. That of subterranean clover, moreover, was higher than that of white clover till 4 days after imbibition but the reverse result was true thereafter. Although germination of Italian ryegrass was delayed with decreased at 200mM of NaCl of $20^{\circ}C$. at 200mM of under$15^{\circ}C$ , and at higher than lOOmh4 of all level of temperature, respectively. Berseem clover had greatest radicle length under the same concentration of NaCl regardless of temperature treatment while radicle elongation of subterranean and white clover was repressed over 50-100mM of NaC1. The slope of linear regression equation between concentration of NaCl and percent germination declined under all temperature treatment in order of berseem, subterranean and white clover whereas the reverse result was showed in radicle length.

• Ecology and Resilient Infrastructure
• /
• v.7 no.4
• /
• pp.248-255
• /
• 2020

In this study, we analyze the current state of domestic food waste (FW) recycling and propose a management plan for greenhouse gas (GHG) emissions. First, the composting potential of the GW demonstrates considerable promise. In particular, the GW (phytoplankton, periphyton, macrophyte, etc.) as a third-generation biomass shows strong performance as a functional additive that mitigates the disadvantages associated with composting FW and improves the quality of the final composted product. Alternatively, the final product (e.g., soil ameliorant) can be used to produce bio-filters that are effective pollutant buffers, with high applicability for green infrastructure. The proposed ecological approaches create new opportunities for FW as a resource for the reduction of GHG emissions, and are expected to contribute to the establishment of effective net-zero carbon systems in the future.

• Journal of Environmental Impact Assessment
• /
• v.32 no.3
• /
• pp.166-175
• /
• 2023

This study aims to identify the cause of the red-water occurrence (the phenomenon of water being red) that occurs at some points and sections of rivers in Yongin City. As a result of conducting a preliminary investigation, total three sites were selected as the investigation point as it was found that the red-water occurrence continued. As a result of the investigation, it is judged that the cause of the red-water in Yongin-city river is due to the soil color and iron content of the region. JPS, SBS, and JJS sites all showed that the color of soil is mainly consist of reddish brown and red-yellow. The average Fe concentration was 13.75 mg/L, 10.85 mg/L, and 1.31 mg/L, for each sites, and considering that the Fe concentration in general river water was less than 0.5 mg/L, it was confirmed that the concentration was quite high. At the JPS and JJS points, the red-water occurrence occurred mainly in stagnant places, which is believed to be strengthened by the reaction of organic and microorganisms. In the case of SBS, the wateris red, but as a result of observing the actual color, it is judged that the iron component deposited in the pipe causes an optical illusion with a deep red color. In addition, it is believed that the iron concentration can be reduced to the general river water concentration range by removing the particulate iron component through a decrease of more than 95% as a result of filtering with glass fiber filter with particulate iron. As a result of this study, it is necessary to manage the river to maintain the flow, and it is believed that the occurrence of red-water at the survey point can be alleviated through uptake action through planting and agglomeration precipitation and agglomeration filtration methods for particulate iron treatment.