• Plant Biotechnology Reports
• /
• v.2 no.1
• /
• pp.47-58
• /
• 2008

To select agronomically useful transgenic plants, a large number of transgenic events are initially produced, gene transfer confirmed, and advanced to obtain homozygous lines for testing in field trials. Direct in planta assays for identifying the transgene carriers in the segregating progeny are based on the activity of selectable marker gene and are easy, simple and inexpensive. For this purpose, expression of bar gene as measured by tolerance to damage by glufosinate ammonium, the active ingredient in the herbicide BASTA, was investigated. Dose damage curves were generated by leaf paint tests with BASTA on four genotypes of sorghum. Transgenic plants were characterized in terms of sensitivity to the concentration of glufosinate ammonium. In transgenics, symptoms of BASTA swab tests at different growth stages and PCR analysis for cry1B were carried out and correlated. Germination tests could not be employed for large scale evaluation of transgenic progeny because of mortality of tolerant seedlings after transplantation to soil. Based on the above findings, a simple, inexpensive, time-saving, two-step scheme for effective evaluation of transgenics and their progeny containing bar gene as selection marker using BASTA swab tests is described.

• Earthquakes and Structures
• /
• v.9 no.2
• /
• pp.339-352
• /
• 2015

Ancient masonry towers constitute a relevant part of the cultural heritage of humanity. Their earthquake protection is a topic of great concern among researchers due to the strong damage suffered by these brittle and massive structures through the history. The identification of the seismic behavior and failure of towers under seismic loading is complex. This strongly depends on many factors such as soil characteristics, geometry, mechanical properties of masonry and heavy mass, as well as the earthquake frequency content. A deep understanding of these aspects is the key for the correct seismic vulnerability evaluation of towers and to design the most suitable retrofitting measure. Recent tendencies on the seismic retrofitting of historical structures by means of prestressing are related to the use of smart materials. The most famous cases of application of prestressing in towers were discussed. Compared to horizontal prestressing, vertical post-tensioning is aimed at improving the seismic behavior of towers by reducing damage with the application of an overall distribution of compressive stresses at key locations.

• Geomechanics and Engineering
• /
• v.31 no.6
• /
• pp. 583-597
• /
• 2022

Frost heave can cause uneven ground uplift that may damage geo-infrastructure. To assist damage-prevention strategies, standard frost heave testing methods and frost susceptibility criteria have been established and used in various countries. ASTM International standard testing method is potentially the most useful standard, as abundant experimental data have been acquired through its use. ASTM International provides detailed recommendations, but the method is expensive and laborious because of the complex testing procedure requiring a freezing chamber. A simple frost heave testing method using a temperature-controllable cell has been proposed to overcome these difficulties, but it has not yet been established whether a temperature-controllable cell can adequately replace the ASTM International recommended apparatus. This paper reviews the applicability of the ASTM International testing method using the temperature-controllable cell. Freezing tests are compared using various soil mixtures with and without delivering blow to depress the freezing point (as recommended by ASTM International), and it is established that delivering blow does not affect heave rate, which is the key parameter in successful characterization of frost susceptibility. As the freezing temperature decreases, the duration of supercooling of pore water shortens or is eliminated; i.e., thermal shock with a sufficiently low freezing temperature can minimize or possibly eliminate supercooling.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.47 no.2
• /
• pp.353-370
• /
• 2023

In this study, the performance of ultrasonic cleaners for ultrasonic washing, which is one of the wet cleaning methods, was evaluated. The washing performances of ultrasonic cleaners were compared with commercial washing machines under normal and wool courses based on different parameters such as detergency, wrinkle formation, fabric damage, dimensional stability, and germ removal ability. Cotton, silk and wool were used in this experiment. Results revealed that the ultrasonic washing detergency of cotton fabrics was lower than that of commercial washing machines under the normal course and similar to that of commercial washing machines under the wool course. Detergency of silk fabrics was similar under both normal and wool courses. In terms of wrinkle formation and dimensional stability, ultrasonic cleaners showed lesser wrinkles and lower shrinkage than commercial washing machines. Considering damage evaluation, ultrasonic cleaners more effectively removed soil without damaging fabrics compared with commercial washing machines. The bacteria removal rate of both cotton and silk was more effective than the wool course of a commercial washing machine. Thus, this study compares the performance of ultrasonic cleaners and commercial washing machines and provides meaningful results related to ultrasonic washing performance.

• Earthquakes and Structures
• /
• v.25 no.2
• /
• pp.135-148
• /
• 2023

The structure-soil-structure interaction (SSSI) effect in adjacent structures may affect the liquefaction-induced damage of shallow foundation structures. The existing studies only analysed the independent effects on the structural dynamic response but ignored the coupling effect of height difference and distance of adjacent structures (F) on liquefied foundations on the dynamic response. Therefore, this paper adopts finite element and finite difference coupled dynamic analysis method to discuss the effect of the F on the seismic response of shallow foundation structures. The results show that the effect of the short structure on the acceleration response of the tall structure can be neglected as F increases when the height difference reaches 2 times the height of the short structure. The beneficial effect of SSSI on short structures is weakened under strong seismic excitations, and the effect of the increase of F on the settlement ratio gradually decreases, which causes a larger rotation hazard. When the distance is smaller than the foundation width, the short structure will exceed the rotation critical value and cause structural damage. When the distance is larger than the foundation width, the rotation angle is within the safe range (0.02 rad).

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.10 no.4
• /
• pp.135-144
• /
• 2006

In performing a risk analysis of structures under earthquakes, it is imperative to identify the vulnerability of structures associated with various damage stages considering structural properties, soil-structure interactions, site condition, and so on. In this paper, the method to derive a representative fragility curve of seismic isolated LRB(lead rubber bearing) bridges is proposed. In which, the curve is assumed log-normally distribution with two parameters. The risk analysis of seismic isolated LRB bridges considering earthquake effects such as PGA, PGV, SA, SV, and SI is also performed to assure the earthquake resisting capability of the structures. An practical way for constructing the representative fragility curves is also recommended combining fragility curves of structures.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.28 no.5
• /
• pp.249-255
• /
• 2024

Strong ground motions at specific sites can cause severe damage to structures. Understanding the influence of site characteristics on the dynamic response of structures is crucial for evaluating their seismic performance and mitigating the potential damage caused by site effects. This study investigates the impact of the average shear wave velocity, as a site characteristic, on the seismic response of low-to-medium-rise reinforced concrete buildings. To explore them, one-dimensional soil column models were generated using shear wave velocity profile from California, and nonlinear site response analyses were performed using bedrock motions. Nonlinear dynamic structural analyses were conducted for reinforced concrete moment-resisting frame models based on the regional information. The effect of shear wave velocity on the structural response and surface ground motions was examined. The results showed that strong ground motions tend to exhibit higher damping on softer soils, reducing their intensity, while on stiffer soils, the ground motion intensity tends to amplify. Consequently, the structural response tended to increase on stiffer soils compared to softer soils.

• Korean Journal of Environment and Ecology
• /
• v.21 no.6
• /
• pp.559-564
• /
• 2007

This research is aimed at finding out the ecological change of Seunghwanglim through the examination of vegetational change of tree seedlings of old giant trees growing at Seunghwanglim [Seunghwang Forest designated as a Natural Monument No.93] after the installation of protective iron fence. The survey results are as follows: First, as a result of keeping out people by installing a protective iron fence in 1989, soil hardness of the plane forest was greatly improved except the area used as roads. The seedlings of the plane forest, whose damage index is 2,3,4, were found to have the highest 1,202 $individuals/400m^2$, and those of the plane forest, whose damage index is 1, were revealed to have 565 $individuals/400m^2$, and those of the slope forest were found to have 403 $individuals/400m^2$. The number of the young sapling[taller than 2 meters] individuals of the plane forest whose damage index is 1 was found to be the most $48/400m^2$, and that of the plane forest whose damage index is 2, 3, 4- was $31/400m^2$, and that of the slope forest proved to be $14/400m^2$. In the plane forest whose damage index is 2, 3, 4, the saplings of the Ulmus davidiana var. japonica and Acer triflorum are relatively much distributed, and in the plane forest whose damage index is 1, many saplings of the Prunus padus and Styrax obassia, were found to grow and in the slope forest, many saplings of the Acer pictum subsp. mono and Acer pseudo-sieboldianum. were found to grow. Many seedlings of Rubus spp. - R. oldhamii, R. coreanus and R. crataegifolius. - and Akebia quinata were growing vigorously on plane forest, but they might decrease in number with the increasing number of the tree saplings.

• Korean Journal of Environment and Ecology
• /
• v.1 no.1
• /
• pp.35-45
• /
• 1987

To survey trail damage and vegetational change around trail at Bukhan Mountain National Park in Korea, field survey was executed over Bukhan mountain district during August, 1987. Trail damage was surveyed for he section of 7.18km from Ui valley to Jeongnung valley in which user's density was high. Ground vegetation was surveyed with a belt-transect method from trail edge to forest and edge species were surveyed with a belt method along trail edge. Interrelation between trail damage and user's density was not dear. Damage class II of trail showed 23.9% of the surveyed section and class III showed 8.0% and class II and III of natural trail showed 19.7% and those of facilitated trail showed 12.3%. The length of damage class II and III requiring readjustment were estimated as 3.65 km and 1.22km from the main trail course of 15.3km at Bukhan mountain district. In case of no intervention to forest by users, vegetational change around trails was appeared up to 6-8 m from trailside. But in case of intervention to forest by users, vegetational change was not coincident with the change of soil hardness and was diverse locally. Quercus mongolica, Lespedeza cyrtobotrya and Rbo-dodendron mucronulatum were appeared as Raunkiaer frequency class E, and Weigela subsessilis. Lespedeza maximowiczii, Rhododendron schlippenbachii and Sorbus alnifolid were appeared as class D at trail edge of ridge-Quercus mongolica was appeared as class E and Rhododenderon mucrounulatium. Stephanandra incisa were appeared as class D at trail edge of midslope. Rhododenron mucronulatum. Lespedeza maximowiczii and Stepanandra incisa were appeared as class D at trail edge of valley. Lespedeza cyrtobotrya. Lespedeza maximowiczii and Stephanandra incisa were appeared as class D at trail edge of valley beside motorway.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.10 no.3
• /
• pp.75-81
• /
• 2008

This study was conducted to analyze the influence of simulated acid rain on leaves of Liliodendron tulipifera seedlings. The seedlings were treated with four levels of simulated acid rain with pH of 5.6, 4.9, 3.9 and 2.9, and then chlorophyll contents and the degree of foliar damage were investigated. Differences were statistically significant among treatments in chlorophyll contents in all of the tested soils. The total contents of chlorophyll tended to decrease as the simulated acid rain treatment was maintained. Chlorophyll contents, however, did not decrease according to the decrement of pH levels. Foliar damage increased as the simulated acid rain was maintained in all tested soils. Especially, the damage dramatically increased between May and July as the acidity increased. As the treatment of simulated acid rain continued, deformed new leaves appeared. In all treatments except the control, the leaves turned brown and the damage increased with time. Necrotic spots appeared during the first month of treatment at the pH level of 2.9 in all soil types. The damage by acid rain should be considered when we plant Liliodendron tulipifera.