• Journal of Soil and Groundwater Environment
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• v.11 no.3
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• pp.66-77
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• 2006

This review inquired the recently applied molecular biological and biochemical methods analyzing the microbial community structure of groundwater and, as a result, summarized the functional or taxonomic groups of active microorganisms with major contaminants in groundwater. The development of gene amplification through PCR has been possible to figure out microbial population and identification. Active microbial community structures have been analyzed using a variety of fingerprinting techniques such as DGGE, SSCP, RISA, and microarray and fatty acid analyses such as PLFA and FAME, and the activity of a specific strain has been examined using FISH. Also, this review included the dominant microflora in groundwater contaminated with fuel components such as n-alkanes, BTEX, MTBE, and ethanol and chlorinated compounds such as TCE, PCE, PCB, CE, carbon tetrachloride, and chlorobenzene.

• Journal of the Korean GEO-environmental Society
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• v.19 no.10
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• pp.13-19
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• 2018

Following the 2016 Gyeongju earthquake, the Pohang Earthquake occurred in 2017, and the south-east region in Korea is under the threat of an earthquake. Especially, in the Pohang Earthquake, the liquefaction phenomenon occurred in the sedimentation area of the coast, and preparation of countermeasures is very important. The soil liquefaction can affect the underground facilities directly as well as various structures on the ground. Therefore, it is necessary to identify the liquefaction risk of facilities and the structures against the possible earthquakes and to prepare countermeasures to minimize them. In this study, we investigated the seismic liquefaction risk about the electric power utility tunnels in the southeast area where the earthquake occurred in Korea recently. In the analysis of seismic liquefaction risk, the earthquake with return period 1000 years and liquefaction potential index are used. The liquefaction risk analysis was conducted in two stages. In the first stage, the liquefaction risk was analyzed by calculating the liquefaction potential index using the ground survey data of the location of electric power utility tunnels in the southeast region. At that time, the seismic amplification in soil layer was considered by soil amplification factor according to the soil classification. In the second stage, the liquefaction risk analysis based on the site response analyses inputted 3 earthquake records were performed for the locations determined to be dangerous from the first step analysis, and the final liquefaction potential index was recalculated. In the analysis, the site investigation data were used from the National Geotechnical Information DB Center. Finally, it can be found that the proposed two stage assessments for liquefaction risk that the macro assessment of liquefaction risk for the underground facilities including the electric power utility tunnel in Korea is carried out at the first stage, and the second risk assessment is performed again with site response analysis for the dangerous regions of the first stage assessment is reasonable and effective.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2001.04a
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• pp.100-107
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• 2001

One major problem in the model testing is the boundary effect and size effect caused by the limit in the size of the container. To overcome this problem, various types of laminar boxes are gradually manufactured and used in the shaking table test, which ideally has zero stiffness to horizontal shear. In this study, a small-scale laminar box is manufactured, which is composed of 6 thin aluminum rectangular hollow plates, and its inside dimensions are 300 mm length by 200 mm width by 350 mm depth. Shaking table tests are performed both with the laminar box and the rigid box under the same conditions, where displacements and accelerations are measured at various points of the box and model ground. As result of analyzing the measured data, during the propagation of input seismic motion from the bottom to the ground surface, the relative displacement of the model ground and the amplification of acceleration is hardly amplified in the rigid box. Because of the effect of stress waves reflecting from the rigid wall, the acceleration is slightly decreased at the edge in the rigid box. The laminar box, manufactured in this study, has a problem in that the soil behavior at the edge of ground surface is affected by the inertia force of the top layer due to its excessive self-weight.

• Journal of Plant Biotechnology
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• v.7 no.3
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• pp.155-160
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• 2005

Haloxylon recurvum (Locally known as Khar) is drought and salt tolerant plant of Thar Desert. This plant is a major biomass producer and has economic and ecological importance for the region. There is need for study on biology, propagation and genetic improvement for utilization of this plant for reclamation of saline soils. We report here on in vitro propagation of Haloxylon recurvum (Moq.) using nodal explant. Secretion of phenolic compound from explants was a major constraint for establishment of culture. This was checked by thorough washing and quick transfer of explant on fresh culture medium. Juvenile nodal explant with leaves was found suitable for culture establishment. Benzy-ladenine($4.0\;{\mu}M$) incorporated in Murashige and Skoog (MS) medium with additives (50 mg/L ascorbic acid and 25 mg/L each of adenine sulphate, arginine and citric acid) induced multiple shoots from nodal explant. Addition of $1.0\;{\mu}M$ naphthalene acetic acid (NAA) in combination with $4.0\;{\mu}M$ BAP improved the growth of axillary shoots. Further shoot amplification was achieved by repeated subculture of mother explants on fresh medium. Forty percent of the micropropagated shoots rooted on half-strength MS medium with $4.0\;{\mu}M$ indolebutyric acid (IBA) and 100 mg/L activated charcoal, at $28{\pm}2^{\circ}C$ and $60\%$ RH. Sixty percent of these plantlets were hardened in green house.

• Tuberculosis and Respiratory Diseases
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• v.67 no.3
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• pp.239-243
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• 2009

Delftia acidovorans is a gram-negative motile rod found ubiquitously in soil and in water. Confirmed isolation from clinical infections is rare, and has been documented mostly in immunocompromised patients or those with indwelling catheters. A 53-year-old man was referred for the evaluation of a huge mass-like lesion found incidentally by chest X-ray. The lesion occupied more than half of the right lung and was diagnosed as a large loculated pleural effusion by CT scan. Bloody pus was drained through a percutaneous catheter, and D. acidovorans, identified by the Vitek GN card and confirmed by amplification of 16S ribosomal RNA and sequencing analysis, was isolated repeatedly from the drained pus. The patient was treated with imipenem/cilastatin to which the organism was sensitive. This is a rare report of chronic empyema associated with D. acidovorans in the respiratory system of an immunocompetent patient.

• Journal of Microbiology
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• v.42 no.2
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• pp.87-93
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• 2004

Twelve mecoprop-degrading bacteria were isolated from soil samples, and their genetic and phenotypic characteristics were investigated. Analysis of 16S rDNA sequences indicated that the isolates were related to members of the genus Sphingomonas. Ten different chromosomal DNA patterns were obtained by polymerase-chain-reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences from the 12 isolates. The isolates were found to be able to utilize the chiral herbicide meco-prop as a sole source of carbon and energy. While seven of the isolates were able to degrade both (R)-and (S)-mecoprop, four isolates exhibited enantioselective degradation of the (S)-type and one isolate could degrade only the (R)-enantiomer. All of the isolates were observed to possess plasmid DNAs. When certain plasmids were removed from isolates MPll, MP15, and MP23, those strains could no longer degrade mecoprop. This compelling result suggests that plasmid DNAs, in this case, conferred the ability to degrade the herbicide. The isolates MP13, MP15, and MP24 were identified as the same strain; however, they exhibited different plasmid profiles. This indicates that these isolates acquired dif-ferent mecoprop-degradative plasmids in different soils through natural gene transfer.

• Journal of the Korean Geotechnical Society
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• v.37 no.12
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• pp.7-23
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• 2021

Underground utility tunnels, which contribute to supply of electricity, communication, water and heat, are critical lifelines of an urban area. In case service is discontinued or functional disruption happens, there will be a huge socio-economic impact. For the improved seismic design and evaluation of underground structures, this study proposes a ground displacement measure when the site is subjected to a scenario earthquake based on hazard-consistent source spectra and site amplification/attenuation. This measure provides a rational estimation of ground displacement and can be an alternative to existing response displacement methods.

• Tunnel and Underground Space
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• v.19 no.6
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• pp.490-497
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• 2009

The horizontal and vertical response spectra using the observed ground motion from the recent 5 macro earthquakes were analysed and then were compared to both the seismic design response spectra(Reg Guide 1.60), applied to the domestic nuclear power plants, and the Korean Standard Design Response Spectrum for general structures and buildings(1997). 74 horizontal and 89 vertical observed ground motions, without considering soil types, were used for normalization with respect to the peak acceleration value of each ground motion. The results showed that the horizontal MPOSD(Mean Plus One Sigma Standard Deviation) response spectra revealed much higher values for the whole frequency bands above 1 Hz than Reg. Guide(1.60). For the vertical response spectra, the results showed slightly higher than just between 7 and 8 Hz frequency band. The results were also compared to the Korean Standard Response Spectrum for the 3 different soil types and showed that the horizontal MPOSD response spectra revealed much higher values for the whole periods below 2 second(0.5 Hz) than those of SE soil type. The vertical response spectra showed similar to the values of the Korean Standard Response Spectrum of SD soil type. These spectral values dependent on frequency could be related to characteristics of the domestic crustal attenuation and the effect of each site amplification. However, through the qualitative improvements and quantitative enhancement of the observed ground motions, the conservation of horizontal seismic design response spectrum should be considered more significantly for the whole frequency bands above the 1 Hz.

• Geophysics and Geophysical Exploration
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• v.13 no.3
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• pp.203-218
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• 2010

The site categorization and corresponding site amplification factors in the current Korean seismic design guideline are based on provisions for the western United States (US), although the site effects resulting in the amplification of earthquake ground motions are directly dependent on the regional and local site characteristic conditions. In these seismic codes, two amplification factors called site coefficients, $F_a$ and $F_v$, for the short-period band and midperiod band, respectively, are listed according to a criterion, mean shear wave velocity ($V_S$) to a depth of 30 m, into five classes composed of A to E. To suggest a site classification system reflecting Korean site conditions, in this study, systematic site characterization was carried out at four regional areas, Gyeongju, Hongsung, Haemi and Sacheon, to obtain the $V_S$ profiles from surface to bedrock in field and the non-linear soil properties in laboratory. The soil deposits in Korea, which were shallower and stiffer than those in the western US, were examined, and thus the site period in Korea was distributed in the low and narrow band comparing with those in western US. Based on the geotechnical characteristic properties obtained in the field and laboratory, various site-specific seismic response analyses were conducted for total 75 sites by adopting both equivalent-linear and non-linear methods. The analysis results showed that the site coefficients specified in the current Korean provision underestimate the ground motion in the short-period range and overestimate in the mid-period range. These differences can be explained by the differences in the local site characteristics including the depth to bedrock between Korea and western US. Based on the analysis results in this study and the prior research results for the Korean peninsula, new site classification system was developed by introducing the site period as representative criterion and the mean $V_S$ to a depth of shallower than 30 m as additional criterion, to reliably determine the ground motions and the corresponding design spectra taking into account the regional site characteristics in Korea.

• The Journal of Engineering Geology
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• v.19 no.1
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• pp.15-23
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• 2009

The site effects relating to the amplification of ground motion under earthquake loading are strongly influenced by both the subsurface soil condition and geologic structure. In this study, the site effects at the Hongseong area in Korea were examined by both the site investigation including borehole drilling and in-situ seismic tests and the site visit for acquiring geologic information of ground surface. Subsurface of Hongseong area with a major instrumental earthquake event in 1978 is composed of weathered layers of a maximum of 45 m thickness overlying bedrock. A geotechnical information system based on GIS framework was implemented to effectively find out spatial geologic structure of study area and it indicated Hongseong is a shallow and wide shaped basin. Two-dimensional finite element (FE) analyses for a representative cross-section of the Hongseong area were performed to evaluate seismic site responses. From the results of seismic responses, it was observed that the ground motions were amplified during the propagation of shear waves through the soil layer overlying the bedrock and the duration of shaking near the basin edges was prolonged due to the surface waves generated by interactions of shear waves with basin geometry. Furthermore, one-dimensional FE seismic response analyses were additionally conducted for soil sites selected in the basin, and it gives similar results to the two-dimensional seismic responses at most locations in the basin with the exception of the locations near the basin edges, because the basin in this study is very shallow and wide.