• Journal of Microbiology and Biotechnology
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• v.7 no.6
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• pp.386-390
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• 1997

A xylanase from the Bacillus sp. strain DSNC 101, isolated from soil, was purified to homogeneity by anion-exchange and hydrophobic interaction chromatography followed by gel filtration chromatography. The enzyme cleaved xylan, but not carboxymethyl cellulose, Avicel, soluble starch, and pNPX. The main product of oat spelts xylan hydrolysates was xylobiose. The xylanase had a molecular weight of 25 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Optimum temperature and pH for the xylanase activity were $50^{\circ}C$ and 6.0, respectively. $K_{m}\;and\;V_{max}$ of the enzyme for oat spelts xylan were 12.5 mg of xylan/ml and 869.5 unit/mg of protein, respectively. Xylanase was completely inhibited by Hg, Cu, and N-bromosuccinimide, but was stimulated by Ca, Co, and Mg.

• Microbiology and Biotechnology Letters
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• v.23 no.3
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• pp.322-328
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• 1995

A thermophilic bacteria showing proteolytic activity against defatted soybean was isolated from soil. It was identified as Bacillus amyloliquefaciens based on its morphological and physiological characteristics. The Bacillus amyloliquefaciens NS 15-4 was cultivated at 50$\circ$C by rotary shaking in a medium containing defatted soybean. An extracellular protease from this strain was purified to homogeneity by ammonium sulfate precipitation, ion exchange, and hydrophobic interaction chromatographies. The molecular weight of the enzyme was estimated to be approximately 30,000 by SDS-PAGE and the N-terminal amino acid sequence of the enzyme was turned out to be AQSVPYGISQIKAPA. The optimum temperature and pH for the enzyme reaction were 60$\circ$C and 11, respectively, and its thermostability was increased by the addition of calcium ion. The enzyme was inactivated by phenylmethylsulfonylfluoride, suggesting it be a serine protease. Comparing with other commercial proteases, the enzyme showed relatively high proteolytic activity against defatted soybean, a water-insoluble protein substrate.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.1209-1211
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• 2003

The wavelet and semivariogram analysis method are used to identify the city landscape and farmland landscape pattern on the 1m resolution IKONOS images. The results prove that wavelet method is a potential way for landscape pattern analysis. Compared to semivariogram analysis, Wavelet analysis can not only detect the overall spatial pattern, but also find multi-scale and direction structures. In this experiment, the wavelet analysis results indicate: (1) the city landscape image is mainly composed of three level structures whose spatial pattern characters appear at 2m, 16m, 128m and 256m accordingly; (2) the farmland landscape is mainly two scale spatial patterns appearing at the 2m, 128m and 256m. IKONOS Remote sensing, with the high spatial and spectral information, is a powerful tool that can use in many ecological systems research and sustainable management.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.165-168
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• 2011

An organic apple (Malus ${\times}$ domestics Borkh.) orchard was established to study the interaction effects of ground cover management systems (GMS) and nutrient sources (NS) on soil and tree nutrient status and tree growth. Trees received one of four GMS: 1) green compost (GC), 2) wood chips (WC), 3) shredded paper (SP), and 4) mow-and-blow (MB). Across all GMS, one of three NS was applied: A) a commercial organic fertilizer (CF), B) poultry litter (PL), and C) control (NF). Overall, GMS had greater effects on the variables than did NS. GC mulch supplied greater nutrients, followed by WC, SP, and MB mulches. SP trees had lower foliar [N] in the first two years than the GC and WC trees. GC-and WC-treated trees had larger trunk cross sectional area than the SP and MB trees.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2006.03a
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• pp.345-350
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• 2006

If a structure is founded on the ground saturated with pore water, then the ground should be modeled as a saturated two-phase porous medium for accurate earthquake response analysis. In this study, a 3-dimensional transmitting boundary is developed for modeling of far field using u-U formulation for water-saturated transversely isotropic layered stratum. The developed transmitting boundary is verified by comparing the dynamic stiffness of rigid square foundation on water-saturated isotropic layered stratum with the case of using equivalent single-phase medium model.

• Structural Engineering and Mechanics
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• v.22 no.1
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• pp.33-52
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• 2006

Three-dimensional description of building structure taking into consideration soil-structure interaction is a very complex problem and solution of this problem is often obtained by using finite element method. However, this method takes a significant amount of computational time and memory. Therefore, an efficient computational model based on subdivision of the structure into building elements such as wall and floor slab elements, plane and three-dimensional joints and lintels, that could provide accurate results with significantly reduced computational time, is proposed in this study for the analysis three-dimensional structures subjected to dynamic load. The examples prove the efficiency and the computing possibilities of the model.

• International Journal of High-Rise Buildings
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• v.9 no.4
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• pp.369-376
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• 2020

Given the importance of quickly recovering livelihoods and economic activity after an earthquake, the seismic performance of the pile foundation is becoming more critical than before. In order to promote seismic retrofit of the pile foundations, it is necessary to develop a method for evaluating the seismic performance of the pile foundation based on the experimental data. In this paper, we focus on the building that was suffered severe damage to the pile foundation, conduct simulation analyses of the building, and report the results of evaluating the dynamic characteristics when piles are damaged using a system identification method. As a result, an analysis model that can accurately simulate the behavior of the damaged building during an earthquake was constructed, and it was shown that the system identification method could extract dynamic characteristics that may damage piles.

• Geomechanics and Engineering
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• v.24 no.4
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• pp.337-348
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• 2021

Brillouin Optical Frequency Domain Analysis (BOFDA) is a distributed fiber optic sensing (DFOS) technique that has unique advantages for performance monitoring of piles. However, the complicated production process and harsh operating environment of offshore PHC pipe piles make it difficult to apply this method to pile load testing. In this study, sensing cables were successfully pre-installed into an offshore PHC pipe pile directly for the first time and the BOFDA technique was used for in-situ monitoring of the pile under axial load. High-resolution strain and internal force distributions along the pile were obtained by the BOFDA sensing system. A finite element analysis incorporating the Degradation and Hardening Hyperbolic Model (DHHM) was carried out to evaluate and predict the performance of the pile, which provides an improved insight into the offshore pile-soil interaction mechanism.

• The Korean Journal of Mycology
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• v.20 no.4
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• pp.337-346
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• 1992

Trichoderma spp. are an effective control agent for damping-off or other plant diseases. The interaction between. T. hamatum and Rhizoctonia solani on the rhizosphere or surface soil were examined to assess the possible roles of antibiosis or competition in the mechanisms of biological control agents as a basic research. In a proportional comparison, total bacteria, fungi, actinomycetes and Trichoderma spp were 65%, 8.8%, 25.9% and 0.28% respectively in their distribution in the soil. Among Trichoderma spp isolated, the 5 species of Trichoderma spp were indentified as T. koninggii, T. pseudokoninggii, T. aureoviridi, T. hamatum and T. viride respectively. In a mycoparasitic test, one isolate of T. hamatum strain Tr-5 showed an enzymatic ability to break fungal hyphae into piecies and infected on the R. solani hyphae showing a parasitism. Spore germination of the all isolates of Trichoderma spp showed a 1.7-7.3% of germination in natural soil conditions, but the percentage was high in sterile soil indicating all the natural soil were fungistatic on conidia of Trichoderma spp. In rhizosphere competent assay in pea plant, the antagonistic T. hamatum, T. viride, T. koninggii, T. pseudokoninggii showed a colonizing upper soil depth in rhizosphere around 1-3 cm in root zone, but the colonizing ability was much reduced along the deeper the soil depth. Propagule density was decreased in deeper the soil layer. Disease development rate treated alone with plant pathogens, Fusarium solani, Rhizoctonia solani, Cylindrocarpon destructans increased, but disease incidence rate reduced in treatment with combinations with antagonistic T. hamatum strain Tr-5.

• Korean Journal of Weed Science
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• v.7 no.3
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• pp.329-336
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• 1987

Antagonistic interaction between glyphosate and some herbicides in effects of post-emergence application on cut grass and of pre-emergence application on some weed species including barnyardgrass was evaluated with pots in plastic house. By addition of oxyfluorfen to glyphosate, the weeding efficacy on cutgrass was visually evaluated as promotive in both weeding rate and efficacy. However, by alachlor, simazine, gluphosinate or quizalofop-ethyl showed delayed weeding rate, and by paraquat, the promotive in rate but lower in efficacy, respectively. Fresh weights of cutgrass at 30 days after the lower-rate application has generally indicated the lower weeding efficacy than that from glyphosate alone. Even at higher rate application, the addition of alachlor or simazine to glyphosate has still indicated the lower efficacies. As a result of interaction analysis by Colby method, no synergism was detected from any treatments, and the combinations of simazine or paraquat to glyphosate in lower mixing rate showed significant antagonism at 5% probability level of DMRT. On the annual weed species including barnyardgrass, the significant soil-residual weeding efficacies were recognized by addition of any rate of oxyfluorfen, alachlor, simazine and higher rate of quizalofop-ethyl to glyphosate, respectively.