• Title/Summary/Keyword: sodium hypochlorite solution

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EVALUATION OF SODIUM DICHLOROISOCYANURATE AS A ROOT CANAL IRRIGATION SOLUTION;Cl- CONCENTRATION, pH, CYTOTOXICITY AND ANTIMICROBIAL EFFECT IN VITRO (이염화이소시아뉼산나트륨 제재의 근관세척액 사용 가능성 평가;염소이온농도, 세포독성, 항균성 및 pH)

  • Lee, Woo-Cheol;Kang, Bong-Sun;Kim, Cheol-Ho;Son, Ho-Hyun
    • Restorative Dentistry and Endodontics
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    • v.28 no.5
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    • pp.425-430
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    • 2003
  • The purpose of this study was to evaluate the clinical applications of the Sodium Dichloroisocyanurate effervescent tablet as a routine root canal irrigant by performing several in vitro tests such as $Cl^{-}$ content. cytotoxicity. antimicrobial effect as well as its pH level compared to the equivalent concentration of sodium hypochlorite solution. 1. Sodium Dichloroisocyanurate demonstrated lower level of $Cl^{-}$ concentration than each dilution of sodium hypochlorite solution. Both solution has increased level of $Cl^{-}$ as the concentration of each solution increased. There was no significant change of $Cl^{-}$ concentration in sodium hypochlorite as time goes by. However. $Cl^{-}$ concentration in Sodium Dichloroisocyanurate was increased. 2. The antimicrobial effects of both solutions were increased when their concentrations were increased. One day after dilution. antimicrobial effect of Sodium Dichloroisocyanurate was slightly higher than sodium hypochlorite. however. there was no difference in 1 week dilution solution. One month dilution solution of sodium hypochlorite still retain its activity. but antimicrobial effect of Sodium Dichloroisocyanurate was drastically decreased 1 month after dilution. 3. The cytotoxicity of Sodium Dichloroisocyanurate was rather higher than same concentration of sodium hypochlorite solution until 1 week after dilution. Then in 1 month. cytotoxicity of Sodium Dichloroisocyanurate was decreased than that of 1 week dilution solution. especially 4% Sodium Dichloroisocyanurate solution has almost no toxicity. However. 1% and 2% sodium hypochlorite solution has unchanged moderate degree of cytotoxicity after the dilution. Furthermore. 4% sodium hypochlorite solution showed high level of toxicity. 4. The pH level of Sodium Dichloroisocyanurate showed that the solution was weak acid (pH5). On the other hand. sodium hypochlorite was revealed as a strong alkaline solution (pH12). There was no change in pH following the dilution of each solution. As results. Sodium Dichloroisocyanurate solution fully satisfy the basic requirements as a root canal irrigation solution. However. we strongly recommend to use this solution clinically in low concentration and try to apply into the root canal within 1 week after dilution.

THE EFFECT OF SOLVENT ACTION OF SODIUM HYPOCHLORITE SOLUTION ON PULP TISSUE (차아염소산(次亞鹽素酸)나트륨용액(溶液)의 치수조직(齒髓組織)에 대(對)한 용해효과(溶解效果))

  • Park, Jin-Hoon
    • Restorative Dentistry and Endodontics
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    • v.8 no.1
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    • pp.115-121
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    • 1982
  • Sodium hypochlorite solution has been widely used as endodontic irrigant due to its ability to dissolve pulp tissue debris and its antimicrobial action. This in vitro study was conducted to evaluate the solvent action of sodium hypochlorite solution on vital pulp tissue under various conditions include concentration, exposure time, and temperature. The percentage of weight loss due to pulp tissue dissolution was calculated with weight difference of lyophilized specimens before and after the exposure to test solutions. The results were as follows; Statistical analysis indicated that the ability of both 5.0% and 2.5% sodium hypochlorite solutions to dissolve pulp tissue was significantly greater than that of distilled water, but no significant difference was found between 5.0% and 2.5% sodium hypochlorite solutions. There was no significant increase in the pulp tissue dissolving ability of sodium hypochlorite solutions; as exposure time increased 2 minutes, 5 minutes, and 10 minutes. Of the given temperatures, no significant difference was found in the solvent aciton of sodium hypochlorite solution on pulp tissue between $20^{\circ}C$ (room temperature) and $37^{\circ}C$(body temperature).

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A STUDY ON THE CLEANSING EFFECT OF GLYOXIDE ON THE DENTINAL WALLS OF ROOT CANAL (Glyoxide의 근관정화효과에 관한 연구)

  • Lim, Sung-Sam
    • Restorative Dentistry and Endodontics
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    • v.14 no.2
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    • pp.103-110
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    • 1989
  • The purpose of this study was to evaluate the cleansing effect of Glyoxide on the dentinal walls of the root canal. Fourty teeth were divided into four groups and the canals in each group were individually enlarged with K-file by step-back technic and irrigated with one of four irrigants. The four used irrigants were Glyoxide (Marion lab. U.S.A.) in combination with 3.5% sodium hypochlorite, 3.5% sodium hypochlorite, 3.5% sodium hypochlorite in combination with 3% hydrogen peroxide and normal saline solution. All the irrigants were used in conjunction with instrumentation as they would be during clinical conditions. After final irrigation, the canals were dried with paper points and the teeth were split longitudinally. The cleaness of canal walls according to the size and the level of canals were evaluated under steroscope by t analysed statistically The results were as follows. 1. The use of 3.5% sodium hyphochlorite in combination with 3.0% hydrogen peroxide revealed the most clean canal surface regardless of the size of canal at apical third of root canal (p < 0.05). 2. Glyoxide in combination with 3.5% sodium hyphchlorite showed no significant difference in cleaning effect of canal surface compared with 3.5% sodium hypochlorite and normal saline solution at the apical third of narrow canal. 3. Glyoxide in combination with 3.5% sodium hypochlorite revealed no significant difference in debridement of canal walls compared with 3.5% sodium hypochlorite in combination with 3% hydrogen peroxide and saline solution at the middle third of narrow and large canals.

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A SCANNING ELECTRON MICROSCOPIC STUDY OF THE EFFICACY OF ROOT CANAL IRRIGATION (근관세척(根管洗滌)에 관(關)한 주사전자현미경적(走査電子顯微鏡的) 연구(硏究))

  • Lee, Myung-Jong
    • Restorative Dentistry and Endodontics
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    • v.8 no.1
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    • pp.147-153
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    • 1982
  • The purpose of this study is to observe the effect of cleansing action of irrigation solutions which are 3% hydrogen peroxide, 5% sodium hypochlorite and 15% EDTA solution on the root canal wall. After the root canal wall is enlarged with K-file in distilled water, the canal wall which is irrigated with each irrigant for 2 minutes, is compared with the. control group without using any irrigants. Each sample is dehydrated, and coated with 200-250${\AA}$ of gold, and observations are made with the use of scanning electron microscope. The results are as follows: 1. The canal walls irrigated with 3% hydrogen peroxide, 5% sodium hypochlorite and 15% EDTA solution are cleaner than the walls without using irrigants. 2. There are no significant difference of cleansing effect among 3% hydrogen peroxide, 5% sodium hypochlorite and 15% EDTA. 3. After using 3% hydrogen peroxide and 5% sodium hypochlorite solution, large debris are removed on root canal walls, but micro debris remain on the canal walls. 4. The root canal walls irrigated with 15% EDTA solution are decalcifed slightly and show clean surfaces.

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SURFACE DISINFECTION OF INTRAORAL FILMS (구내 방사선 필름의 표면소독효과에 관한 연구)

  • Lee Jin-Koo;Park Tae-Won
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.22 no.2
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    • pp.329-335
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    • 1992
  • The purpose of the study was to determine whether Sodium hypochlorite and Glutaraldehyde would be effective for the surface disinfection of contaminated radiographic film pockets with saliva The following results were as obtained 1. Proper times for surface disinfection of 2.0% Glutaraldehyde and 3.5% Sodium hypochlorite were 60 seconds. 2. When films were immerged in 2% Glutaraldehyde solution for 1 minute, baterial colonies were present in 24 cases(80%). 3. When films were immerged in 3.5% Sodium hypochlorite solution for 1 minute, bacterial colony was absent in 25 cases(83.3%). 4. Differences of effectiveness on surface disinfection between 2% Glutaraldehyde and 3.5% Sodium hypochlorite were statistically significant.

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The Effects of Chlorination on the Friction Properties of SBR (염소화 반응조건이 SBR의 표면마찰 특성에 미치는 영향)

  • Park, Cha-Cheol;Kim, Ho-Jung
    • Fashion & Textile Research Journal
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    • v.10 no.1
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    • pp.101-105
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    • 2008
  • This study was concerned with the influence of reaction conditions on the surface friction properties of Syrene-Butadiene Rubber(SBR) sheet when it was chlorinated by chemical treatment method using the sodium hypochlorite and sulfuric acid. The results of this study were as follows. SEM photographs of chlorinated SBR surface showed uneven etching caused by the chlorination. In the FTIR spectra, the intensity of C=C peak of SBR was decreased with increasing the concentration of sodium hypochlorite. Otherwise there was no trace of C=C peak in spectrum of SBR treated with 5 wt% NaOCl with pH 0.1 for 90 seconds. EDX spectra showed that relative content of chlorine of SBR was increased with increasing the amount of sodium hypochlorite, and also affected with pH condition of acid solution. Friction angle and friction coefficient of SBR were influenced with concentration of sodium hypochlorite, but were not with pH condition of acid solution.

Chlorhexidine gel associated with papain in pulp tissue dissolution

  • Couto De Oliveira, Gabriel;Ferraz, Caio Souza;Andrade Junior, Carlos Vieira;Pithon, Matheus Melo
    • Restorative Dentistry and Endodontics
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    • v.38 no.4
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    • pp.210-214
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    • 2013
  • Objectives: This study aimed to evaluate the capacity of 2% chlorhexidine gel associated with 8% papain gel in comparison with 5.25% sodium hypochlorite in bovine pulp tissue dissolution. Materials and Methods: Ninety bovine pulps of standardized sizes were used and fragmented into 5-mm sizes. The fragments were removed from the root middle third region. They were divided into 6 experimental groups (n = 15), 1) 8% papain; 2) 2% chlorhexidine; 3) 2% chlorhexidine associated with 8% papain; 4) 0.9% saline solution; 5) 2.5% sodium hypochlorite; and 6) 5.25% sodium hypochlorite. The pulp fragments were weighed and put into immobile test tubes for dissolution for time intervals of 30, 60, 90, and 120 min. Results: The 5.25% sodium hypochlorite had greater dissolution potential than the pure papain, and when associated with chlorhexidine, both promoted greater dissolution than did the saline solution and 2% chlorhexidine groups (p < 0.05). The 2.5% sodium hypochlorite promoted dissolution to a lesser extent than the groups with papain within a period of 30 min (p < 0.05), but, was comparable to the saline solution and chlorhexidine. After 120 min, the 2.5% and 5.25% sodium hypochlorite promoted dissolution of 100% of the pulp fragments, and papain, 61%, while chlorhexidine associated with papain and chlorhexidine alone dissolved only 55% and 3%, respectively. Conclusions: The 8% papain in gel, both alone and in association with chlorhexidine, was able to dissolve bovine pulp tissue, but to a lesser extent than did 5.25% sodium hypochlorite.

Effects of Light Source, Plant Growth Regulators (GA, BA, ABA) and Sodium Hypochlorite on 'Grand Rapid' Lettuce (Lactuca Sativa L.) Seed Germination (광질(光質), 생장조절물질(生長調節物質) (GA, BA, ABA) 및 Sodium Hypochlorite의 처리가 상치 (Lactuca sativa L.) 종자의 발아(發芽)에 미치는 영향(影響))

  • Lee, Young Bok;Kim, Young Rae
    • Korean Journal of Agricultural Science
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    • v.10 no.2
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    • pp.242-248
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    • 1983
  • The effects of light source, plant growth regulators (GA, BA, ABA), and sodium hypochlorite (4% Cl, SH) on germination of 'G rand Rapid' lettuce (Lactuca sativa L.) seed were studied. Seed did not germinate under the conditions of dark and blue light. The treatment of GA or BA did not promote on germination under dark or blue light. The germination percentage under red light was very high, it was similar to the condition of white light. ABA inhibited lettuce seed germination. Although ABA was treated, BA 10ppm treatment was moderately effective under white light condition and BA 10ppm or 50ppm combined with GA 50ppm were also effective on seed germination under dark condition. Presoaking in sodium hypochlorite solution induced germination of lettuce seed in any condition of dark or white light. The optimum presoaking time in sodium hypochlorite solution was twenty min.

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Effectiveness of calcium hypochlorite, quaternary ammonium compounds, and sodium hypochlorite in eliminating vegetative cells and spores of Bacillus anthracis surrogate

  • Yim, Jin-Hyeok;Song, Kwang-Young;Kim, Hyunsook;Bae, Dongryeoul;Chon, Jung-Whan;Seo, Kun-Ho
    • Journal of Veterinary Science
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    • v.22 no.1
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    • pp.11.1-11.7
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    • 2021
  • Background: The spore-forming bacterium Bacillus anthracis causes anthrax, an often-fatal infection in animals. Therefore, a rapid and reliable strategy to decontaminate areas, humans, and livestock from B. anthracis is very critical. Objectives: The aim of this study was performed to evaluate the efficacy of sodium hypochlorite, calcium hypochlorite, and quaternary ammonium compound (QAC) sanitizers, which are commonly used in the food industry, to inhibit spores and vegetative cells of B. anthracis surrogate. Methods: We evaluated the efficacy of sodium hypochlorite, calcium hypochlorite, and a QAC in inhibiting vegetative cells and spores of a B. anthracis surrogate. We treated a 0.1-mL vegetative cell culture or spore solution with 10 mL sanitizer. The samples were serially diluted and cultured. Results: We found that 50 ppm sodium hypochlorite (pH 7), 1 ppm calcium hypochlorite, and 1 ppm QAC completely eliminated the cells in vegetative state. Exposure to 3,000 ppm sodium hypochlorite (pH 7) and 300 ppm calcium hypochlorite significantly eliminated the bacterial spores; however, 50,000 ppm QAC could not eliminate all spores. Conclusions: Calcium hypochlorite and QAC showed better performance than sodium hypochlorite in completely eliminating vegetative cells of B. anthracis surrogate. QAC was ineffective against spores of the B. anthracis surrogate. Among the three commercial disinfectants tested, calcium hypochlorite most effectively eliminated both B. anthracis vegetative cells and spores.

Sodium Hypochlorite Solution As a Chemical Wounding Agent for Improving Agrobacterium-mediated Chinese Cabbage Seed Transformation (Sodium hypochlorite처리에 따른 배추종자의 Agrobacterium이용 형질전환 증대)

  • Shin Dong-Il;Park Hee-Sung
    • Journal of Life Science
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    • v.15 no.6 s.73
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    • pp.1034-1036
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    • 2005
  • Chinese cabbage (Brassica campestris ssp. napus var. pekinensis Makino) seeds/seedlings were transformed via vacuum-infiltration with recombinant Agrobacterium tumefaciens LBA4404 cells. The agroinfiltration method was determined to be unsuccessful for Chinese cabbage transformation during the analysis of hepatitis B surface antigen expression by ELISA. However, treatment of sodium hypochlorite solution, prior to agroinfiltration, to pregerminated or germinating 1 day- or 2 days-old seeds was proven effectively to enhance transformation efficiency, suggesting that chemical wounding caused by sodium hypochlorite reaction might facilitate Agrobacterium infection and, therefore, transient gene expression in Chinese cabbage sprouts.