• Journal of the korean academy of Pediatric Dentistry
• /
• v.51 no.3
• /
• pp.290-298
• /
• 2024

This study aimed to evaluate the antibacterial efficacy of erythrosine-mediated photodynamic therapy (PDT) against Streptococcus mutans (S. mutans) in dentin and its effect on the shear bond strength (SBS) of composite resin to dentin. Eighty extracted human noncarious premolars were used in this study. Forty teeth were used for the antibacterial activity test, while the remaining 40 were used for the SBS test. Both experiments were conducted with 4 experimental groups (n = 10): control (distilled water), sodium hypochlorite (NaOCl, 6%), chlorhexidine (CHX, 0.12%), and erythrosine-mediated PDT. Antibacterial effects were evaluated by counting S. mutans colony-forming units (CFUs). The SBS of composite resins to dentin was measured using a universal testing machine. All treatments (NaOCl, CHX, and PDT) demonstrated statistically significant differences in antibacterial activity compared with the control group (p < 0.05). The antibacterial effects were ranked from strongest to weakest as follows: NaOCl, PDT, and CHX. In the SBS test, the NaOCl group exhibited a statistically significant difference compared with the CHX, PDT, and control groups (p < 0.05), with the lowest bond strength. No statistically significant differences were found among the CHX, PDT, and control groups (p > 0.05). Erythrosine-mediated PDT exhibited significant antibacterial effects against S. mutans, with higher antibacterial activity than CHX but lower than NaOCl. Only NaOCl negatively affected the bond strength of composite resin to dentin. In conclusion, erythrosine-mediated PDT shows potential as a cavity disinfectant due to its significant antibacterial effects against S. mutans and lack of adverse effects on bond strength.

• Restorative Dentistry and Endodontics
• /
• v.39 no.2
• /
• pp.95-103
• /
• 2014

Objectives: This study evaluated the effect of three antioxidizing agents on pullout bond strengths of dentin treated with sodium hypochlorite. Materials and Methods: Root canals of 75 single-rooted human teeth were prepared. Fifteen teeth were irrigated with normal saline for a negative control group, and the remaining 60 teeth (groups 2 - 5) with 2.5% NaOCl. The teeth in group 2 served as a positive control. Prior to post cementation, the root canals in groups 3 - 5 were irrigated with three antioxidizing agents including 10% rosmarinic acid (RA, Baridge essence), 10% hesperidin (HPN, Sigma), and 10% sodium ascorbate hydrogel (SA, AppliChem). Seventy-five spreaders (#55, taper .02, Produits Dentaires S.A) were coated with silica and silanized with the Rocatec system and ceramic bond. All the prepared spreaders were cemented with a self-adhesive resin cement (Bifix SE, Voco Gmbh) in the prepared canals. After storage in distilled water (24 h/$37^{\circ}C$), the spreaders were pulled out in a universal testing machine at a crosshead speed of 1.0 mm/min. Pull-out strength values were analyzed by one-way ANOVA and Tukey's HSD test (${\alpha}$ = 0.05). Results: There were significant differences between study groups (p = 0.016). The highest pullout strength was related to the SA group. The lowest strength was obtained in the positive control group. Conclusions: Irrigation with NaOCl during canal preparation decreased bond strength of resin cement to root dentin. Amongst the antioxidants tested, SA had superior results in reversing the diminishing effect of NaOCl irrigation on the bond strength to root dentin.

• Restorative Dentistry and Endodontics
• /
• v.6 no.1
• /
• pp.51-62
• /
• 1980

A Scanning Electron Microscope study was conducted to compare the effectiveness of mechanical preparation of the root canal in conjunction with a few number of canal irrigants that have been widely used for canal treatment. The irrigants used in this study were 5% sodium hypochlorite, 3% hydrogen peroxide, 15% EDTA, and 30% hydrochloric acid. The root canals of 84 freshly extracted teeth with single or multi root were conventionally prepared with Hedstroem files. 78 canals were irrigated with normal saline solution following each instrument number and 6 canals were prepared without irrigation. After instrumentation 72 canals were flushed with various irrigants for predetermined length of time as shown on the Table 1. Additional 20 teeth were kept uninstrumented and five of them were immersed in 15% EDTA for 5 minutes, five in 5% sodium hypochlorite for 5 minutes, five in 3% hydrogen peroxide for 20 minutes, and the last five were only rinsed with saline solution. The SEM examination revealed as follows: 1. The canal wall cannot be thoroughly prepared by means of files. 2. No typical structural changes occured on instrumented dentin surface by saline solution, 3% $H_2O_2$, 5% NaOCl, within 5 minutes. 3. 5% NaOCl Solution showed excellent solvent effect to organic substances in uninstrumented canal within 5 minutes and 15% EDTA and 3% $H_2O_2$ showed unsignificant changes. 4. 15% EDTA and 30% HCl dissolved calcified debris and dentin chips that obturated the dentinal tubules and showed patent orifices. 5. 15% EDTA affected on peritubular dentin more readily and showed concavity around dentinal tubules.

• Membrane Journal
• /
• v.30 no.2
• /
• pp.138-148
• /
• 2020

The purpose of this study was to identify the cleaning efficiency of fouled multi-bore hollow fiber membranes after purification of contaminated water. The PSf (polysulfone) based hollow fiber membrane manufactured by Pure & B Tech Co., Ltd. Was used in this study. The antifouling characteristics during the water treatment were studied using bovine serum albumin (BSA) as a model compound and the chemical resistance was evaluated after long-term impregnation in sodium hypochlorite (NaOCl) solution and Citric acid to understand the long term stability of the membranes. Water permeability and mechanical strength of the membranes after prolonged chemical exposure was measured to observe the change in mechanical stability and long term performance of the membrane. moreover, the recovery efficiency was also evaluated after chemical enhanced backwashing of a membrane contaminated with bovine serum albumin. The PSf hollow fiber membrane exhibited excellent chemical resistance, and it was confirmed that the efficiency of sodium hypochlorite was high as a result of chemical enhanced backwashing.

• Restorative Dentistry and Endodontics
• /
• v.49 no.3
• /
• pp.25.1-25.9
• /
• 2024

Objectives: This study aimed to evaluate the color stability and solubility of Biodentine and NeoPutty in contact with different irrigation solutions. Materials and Methods: Biodentine and NeoPutty were set in cylindrical molds with 7 mm diameter and 1.5 mm high and immersed in distilled water, 17% ethylenediaminetetraacetic acid (EDTA), 2% chlorhexidine (CHX), 9% 1-hydroxyethylidene 1,1-diphosphonate (HEDP), and 5% sodium hypochlorite (NaOCl) solutions for 24 hours. The color change was measured with a spectrophotometer. The solubility values were calculated as the mass loss was expressed as a percentage of the original mass using an analytical balance with 10^-4 g accuracy. Data were analyzed with Kruskal-Wallis followed by Mann-Whitney U tests, and 2-way analysis of variance test followed by Bonferroni corrections for pairwise comparisons for solubility and color stability with a 5% significance threshold, respectively. Results: Biodentine exhibited higher color changes compared to the NeoPutty contact with all solutions except distilled water (p < 0.05). Both hydraulic cements (HCs) showed higher discoloration values immersion in CHX followed by NaOCl. No statistically significant difference was found between Biodentine and NeoPutty regardless of irrigation solution in terms of solubility (p > 0.05). Solubility values were lower in the distilled water group compared to EDTA and CHX (p < 0.05). Conclusions: Tested HCs showed solubility and color changes at various rates. NeoPutty could be an appropriate material in aesthetic areas. The usage of HEDP as an irrigant solution can be considered suitable for various endodontic treatments due to its relatively lower solubility and discoloration values.

• Journal of Plant Biotechnology
• /
• v.42 no.3
• /
• pp.239-244
• /
• 2015

Calanthe striata f. sieboldii Decne. ex Regel is a terrestrial orchid with beautiful flowers arranged in racemose inflorescences. This species is threatened due to over-collection and loss of suitable habitats. Asymbiotic germination is useful in the conservation efforts to re-establish plants in the wild, and for commercial propagation. In this study, we investigate the effects of sodium hypochlorite (NaOCl), three types of culture media (Phytomax Orchid Maintenance - POM, Seed Germination Maintenance - SGM, and Murashige and Skoog 1962 - MS), and plant growth regulators on embryo swelling, protocorm formation, and embryo diameter of C. striata f. sieboldii. Treatment with 1% NaOCl for 30 min greatly enhanced embryo swelling (28.3%), embryo diameter ($205.8{\mu}m$), and embryo protocorm formation (54.8%) compared to seeds without NaOCl treatment (embryo swelling 8.5%, embryo diameter $14.6{\mu}m$, and protocorm formation 13.4%) on POM medium. Protocorm formation on POM medium supplemented with 1.0 mg/L N6-benzyladenine (BA) (95.6%) was better than the control (54.5%). Additionally, the effects of activated charcoal (AC) and sucrose on seedling growth in in vitro culture were examined. The protocorm converted into healthy plants with well-developed shoot primordia on the POM medium with AC and sucrose. The most suitable conditions for seedling growth after 10 weeks of culture were the POM medium with AC or sucrose. These results show effective asymbiotic seed germination and in vitro seedling growth of C. striata f. sieboldii.

• Restorative Dentistry and Endodontics
• /
• v.42 no.1
• /
• pp.54-59
• /
• 2017

Objectives: The purpose of this study was to compare the antibacterial activity of urushiol against Enterococcus faecalis (E. faecalis) to that of NaOCl. Materials and Methods: The canals of thirty two single rooted human teeth were instrumented with Ni-Ti files (ProTaper Next X1, X2, X3, Dentsply). A pure culture of E. faecalis ATCC 19433 was prepared in sterile brain heart infusion (BHI) broth. The teeth were submerged in the suspension of E. faecalis and were incubated at $37^{\circ}C$ for 7 days to allow biofilm formation. The teeth were randomly divided into three experimental groups according to the irrigant used, and a negative control group where no irrigant was used (n = 8). Group 1 used physiologic normal saline, group 2 used 6% NaOCl, and group 3 used 10 wt% urushiol solution. After canal irrigation, each sample was collected by the sequential placement of 2 sterile paper points (ProTaper NEXT paper points, size X3, Dentsply). Ten-fold serial dilutions on each vials, and 100 µL were cultured on a BHI agar plate for 8 hours, and colony forming unit (CFU) analysis was done. The data were statistically analyzed using Kruskal-Wallis and Mann-whitney U tests. Results: Saline group exhibited no difference in the CFU counts with control group, while NaOCl and urushiol groups showed significantly less CFU counts than saline and control groups (p < 0.05). Conclusions: The result of this study suggests 10% urushiol and 6% NaOCl solution had powerful antibacterial activity against E. faecalis when they were used as root canal irrigants.

• Archives of Plastic Surgery
• /
• v.35 no.6
• /
• pp.748-750
• /
• 2008

Purpose: Chemical burn accounts for a small proportion of burns in burn patient population. However, chemical injuries own importance to their deep tissue destruction which continues long after the initial exposure. $YUHANROX^{(R)}$ is a domestic bleach which has been widely used in Korea. Chemical burn by the domestic bleach is an unusual case. Methods: A 70 yrs old female soiled with stool, her husband wiped the mess with $YUHANROX^{(R)}$. Skin edema, erythematous lesion was developed the second day, and she was admitted to our hospital via emergency department. Results: Complete epithelization was done after 3 times STSG but, severe scar was formed. Conclusion: We report this case to warn about the dangers of domestic bleaches chemical substances and to emphasize that they should be used with caution. Public relation of the primary care of the chemical burn injury is needed.

• Restorative Dentistry and Endodontics
• /
• v.10 no.1
• /
• pp.55-62
• /
• 1984

This experimental study was made to evaluate the efficacy of root canal preparation of engine reamer versus hand reamer. Eighty extracted human teeth were prepared with the following treatments and devided into 4 groups; Group 1: Canal preparation with hand reamer, and irrigation with normal saline solution. Group 2: Canal preparation with engine reamer, and irrigation with normal saline solution. Group 3: Canal preparation with hand reamer, and irrigation with 3% hydrogen peroxide and 3.5% sodium hypochlorite solution. Group 4: Canal preparation with engine reamer, and irrigation with 3% hydrogen peroxide and irrigation with 3% hydrogen peroxide and 3.5% sodium hypochlorite solution. After decalcification, 5p. sections at levels 1-, 3-, and 5-mm from the apex were evaluated microscopically. The results were as follows; The effectiveness of hand reamer and engine reamer were equal in preparing the root canal at all levels from the apex, and were equal in normal saline solution groups, and 3% $H_2O_2$ and 3.5% NaOCl solution groups. Both in hand reaming groups and in engine reaming groups, some canal walls had still untouched portion, and canal irregularities and debris were still remained.

• Journal of fish pathology
• /
• v.13 no.2
• /
• pp.97-102
• /
• 2000

In order to investigate the effect of chlorine disinfectant against white spot baculovirus (WSBV), 5, 10, or 30 ppm of sodium hypochlorite (NaOCl) was treated to the WSBV-infected shrimp, Penaeus chinensis. In contrast with the non-treated control, no shrimp was dead after of sodium hypochlorite treatment. This result indicated that WSBV was inactivated by chlorine treatment. No inactivation of WSBV was observed by 10, 20, 30 ppm of povidon-iodine treatment. WSBV was also inactivated by 2, 4 hr sunlight exposure and by 1, 2, 3 hr drying. WSBV was inactivated very effectively by addition of fresh water on sea water.