Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Asymbiotic germination and seedling growth of Calanthe striata f. sieboldii Decne. ex Regel

금새우난초(Calanthe striata f. sieboldii Decne. ex Regel)종자의 비공생 발아 및 신초증식

  • Received : 2015.05.22
  • Accepted : 2015.09.15
  • Published : 2015.09.30
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Abstract

Calanthe striata f. sieboldii Decne. ex Regel is a terrestrial orchid with beautiful flowers arranged in racemose inflorescences. This species is threatened due to over-collection and loss of suitable habitats. Asymbiotic germination is useful in the conservation efforts to re-establish plants in the wild, and for commercial propagation. In this study, we investigate the effects of sodium hypochlorite (NaOCl), three types of culture media (Phytomax Orchid Maintenance - POM, Seed Germination Maintenance - SGM, and Murashige and Skoog 1962 - MS), and plant growth regulators on embryo swelling, protocorm formation, and embryo diameter of C. striata f. sieboldii. Treatment with 1% NaOCl for 30 min greatly enhanced embryo swelling (28.3%), embryo diameter ($205.8{\mu}m$), and embryo protocorm formation (54.8%) compared to seeds without NaOCl treatment (embryo swelling 8.5%, embryo diameter $14.6{\mu}m$, and protocorm formation 13.4%) on POM medium. Protocorm formation on POM medium supplemented with 1.0 mg/L N6-benzyladenine (BA) (95.6%) was better than the control (54.5%). Additionally, the effects of activated charcoal (AC) and sucrose on seedling growth in in vitro culture were examined. The protocorm converted into healthy plants with well-developed shoot primordia on the POM medium with AC and sucrose. The most suitable conditions for seedling growth after 10 weeks of culture were the POM medium with AC or sucrose. These results show effective asymbiotic seed germination and in vitro seedling growth of C. striata f. sieboldii.

본 연구는 자생 난초과식물인 금새우난초의 기내 비대배 유도, 발아 및 배직경에 미치는 몇 가지 영향(NaOCl, 배지, 식물생장조절제)을 조사하기 위해 수행되었다. 1% NaOCl을 30분간 처리한 후 POM배지에 배양할 경우, 비대배 유도는 28.3%, 발아율은 54.8%, 배직경은 $205.8{\mu}m$로 가장 높게 조사되었다. NaOCl 무처리 종자를 POM배지에 배양할 경우, 비대배 유도는 8.5%, 발아율은 13.4%, 배직경은 $14.6{\mu}m$로 조사되었다. 또한 식물생장조절물질의 처리가 발아에 미치는 영향을 조사한 결과, 1.0 mg/L의 BA 처리구(95.6%)의 발아율은 대조구(54.5%)에 비해 1.75배 높게 조사되었다. AC와 sucrose의 처리 농도가 기내 식물체 생장에 미치는 영향을 조사한 결과, 엽장은 10 g/L의 sucrose 처리구에서 6.8 cm로 가장 길었고, 대조구는 3.3 cm로 가장 짧았다. 근장은 50 g/L의 sucrose 처리구에서 5.8 cm로 가장 길었고, 대조구는 1.7 cm로 가장 짧았다. 생중량과 건중량은 AC와 sucrose 처리구가 대조구에 비해 2~5배 이상 높은 결과를 보였다. 결과적으로 본 연구는 금새우난초의 기내 발아 및 증식에 미치는 몇 가지 요인에 관해 구체적인 결과를 제시하였고, 이러한 결과는 향후 자생 난초과식물의 증식 및 보존전략 개발에 중요한 기초자료로 제공 될 것이다.

Keywords

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