• The Journal of Korean Medicine
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• v.25 no.4
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• pp.108-120
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• 2004

Objective : Euonymus alatus (Thunb.) Sieb (EA) is a traditional Korean herbal medicine, commonly used to treat tumors in Korea and China for centuries. Several earlier studies have indicated that EA exhibits anti-tumor properties, but its mechanism remains to be elucidated. In this study, we evaluated the molecular mechanism of EA in a human uterine leiomyomal smooth muscle cell (ULSMC) line. Methods : This study was evaluated by: (a), morphological changes by using acridine orange/ethidium bromide staining; (b), DNA fragmentation by TdT-mediated dUTP nick end labeling (TUNEL); and (c), sub-G1 cell analysis. Results : This study observed that EA treatment caused apoptotic cell death and depletion of intracellular glutathione (GSH) and that reduction of mitochondrial membrane potential was found to be involved in the initiation of apoptosis by EA. Conclusion : This results show that EA exerted clear cytotoxic effects and strongly inhibited the proliferation of ULSMC.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.1
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• pp.219-230
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• 1995

The flow characteristics of a two-dimensional offset jet issuing parallel to a rough wall is experimentally investigated by using a split film probe with the modified Stock's calibration method. The mean velocity and turbulent stresses profiles in the up and down-stream locations of the wall-attachment regions are measured and compared with those of the smooth wall attaching offset jet cases. It is found that the wall-attachment region on the rough wall is wider than on the smooth wall for the same offset height and the jet speed. The position of the maximum velocity point is farther away from the wall than that for the smooth wall case because of the thick wall boundary layer established by the surface roughness. It is concluded that the roughness of the wall accelerates the relaxation process to a redeveloped plane wall jet and produces a quite different turbulent diffusion behavior especially near the wall from comparing with the smooth plane wall jet turbulence.

• Applied Microscopy
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• v.26 no.3
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• pp.293-303
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• 1996

This paper aims to probe that the effect of high dose of cyclophosphamide to the Sertoli cells of the mouse was examined by transmission electron microscope. In the normal group, Sertoli cells contact each other around the basal aspect of the seminiferous tubule, forming numerous row of tight junction, blood-testis barrier. Sertoli cells contain smooth endoplasmic reticulum, well developed Golgi comples, a number of round mitochondria and microfilament. The cytoplasmic necrosis are observed from the 1-time treated group. In the 2-times treated group, smooth endoplasmic reticulum are more developed than normal group, but cisternae are partially dilated. In the 3-times treated group, the smooth endoplasmic reticulum are not developed. In the 2-times treated group, the inner membrane of the mitochondria are partially disrupted, and cristae are all disrupted in the 3-times treated group. The microfilaments are not observed in the all treated groups. According to the results above, it seems that smooth endoplasmic reticulum, mitochondria, and microfilament are disrupted by toxic effects of the cyclosphamide to the Sertoli cells of the mouse.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.2
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• pp.283-288
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• 2005

Packet transmission and independent addressing in the mobile environments are very similar to the ones in the multicasting environment. So, in this paper, we propose a new scheme about how to manage the multicasting group and to set the communication path in the mobile environment by applying two-level addressing mode, which is similar to mobile If, for location-independent address setting. And we propose the smooth handoff scheme that minimizes the handoff delay for mobile multimedia services. To check the performance of our proposed scheme, we modified the NS-2 network simulator and as a result, we showed that our proposed scheme is better than other techniques in terms of handoff delay and transmitted packets' throughput.

• Journal of Nutrition and Health
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• v.49 no.1
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• pp.59-62
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• 2016

Purpose: Zinc, a biomineral present within and outside cells, manages various cellular mechanisms. In this study, we examined whether zinc was involved in vascular smooth muscle cell (VSMC) calcification via regulation of calcification inhibitor protein, osteopontin (OPN). Methods: Rat aorta cell line (A7r5 cells) and primary vascular smooth muscle cells (pVSMCs) from rat aorta were cultured with phosphate (1-5 mM) and zinc ($0-15{\mu}M$) as appropriate, along with osteoblasts (MC3T3-E1) as control. The cells were then stained for Ca and P deposition for calcification examination as well as osteopontin expression as calcification inhibitor protein was measured. Results: Both Ca and phosphate deposition increased as the addition of phosphate increased. In the same manner, the expression of osteopontin was upregulated as the addition of phosphate increased in both cell types. When zinc was added, Ca and P deposition decreased in VSMCs, while it increased in osteoblasts. Conclusion: The results imply that zinc may prevent VSMC calcification by stimulating calcification inhibitor protein OPN synthesis in VSMCs.

• Journal of Veterinary Clinics
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• v.23 no.4
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• pp.421-426
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• 2006

The effects of transmural nerve stimulation induced releasing neurotransmitters on the changes of swine uterine smooth muscle motility were examined by polygraph through isometric force transducer. The frequency dependent relaxation and rebound contraction were revealed on precontraction with histamine by transmural nerve stimulation. The rebound contraction by transmural nerve stimulation was inhibited by nonselective ${\alpha}$-adrenergic receptor antagonist, phentolamine, and the relaxation by transmural nerve stimulation was blocked by nonselective ${\beta}$-adrenergic receptor antagonist, propranolol. The relaxation induced by nonselective ${\beta}$-adrenergic receptor agonist, isoproterenol on precontraction with histamine were the dose dependent manner and this relaxation was blocked by nonselective ${\beta}$-adrenergic receptor antagonist, propranolol in isolated uterine smooth muscle of pig. These results suggest that endogenous neurotransmitters on smooth muscle relaxation was influenced by ${\beta}$-adrenergic receptor in swine.

• Korean Journal of Veterinary Service
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• v.18 no.3
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• pp.29-35
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• 1995

The effects of prostaglandin $F_2{\alpha}$ were investigated on the uterine smooth muscle motility in the pig. The results were summarized as follows : 1. Prostaglandin $F_2{\alpha}$ caused the contraction of the porcine uterine smooth muscle and the contractile responses increased between the concentration of prostaglandine $F_2{\alpha}$ $10^{-9}$ M and $5{\times}10^{-8}$ M with a dose-dependent manner. 2. The contractile response induced by prostaglandine $F_2{\alpha}$($10^{-8}$ M) was not blocked by pre-treatment with cholinergic receptor blocker, atropine ($10^{-6}$ M). 3. The contractile response induced by prostaglandine $F_2{\alpha}$(10$^{-8}$ M) was not blocked by pretreament with ${\alpha}$-adrenergic receptor blocker, phentolamine($10^{-6}$ M) and ${\beta}$-adrenergic receptor blocker, propranolol($10^{-6}$ M). From these results, it was concluded that the effects of uterine smooth muscle by prostaglandine $F_2{\alpha}$ were only the contraction mediated by prostaglandine TEX>$F_2{\alpha}$ receptor in pig, and that it may not be related to the cholinergic and adrenergic receptor.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.2
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• pp.210-217
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• 1999

This paper reports the experimental results on heat transfer characteristics of R-22 and R-407C(HFC-32/125/134a 23/25/52 wt%) condensing inside horizontal smooth and finned tubes. The test condensers used In the study are double pipe heat exchangers of 7.5 mm ID, 9.5 mm OD smooth tube, and 60 finned micro-fin tube with 8.53 mm ID, 9.53 mm OD. Each of these tubes was 4 000 mm long tubes connected with an U-bend. These U type two-path test tubes are divided In 8 local test sections for the identification of the local condensing heat transfer characterisitcs and pressure drop, U-bend effects on condensing flows. Inlet quality is maintained 1.0, and refrigerant mass velocity is varied from 102.0 to $301.0kg/m^2{\cdot}s$. From the results, it was found that the pressure drop of the R-407C Increased, and heat transfer coefficient decreased compared to those of R-22. In comparison condensing heat transfer characteristics of micro-fm tube with those of smooth tube, increasing of condensing heat transfer coefficient was found outstanding compared to the increasing ratio of pressure drop. Furthermore, pressure drop In U-bend showed at most a 30 % compared to the total pressure drop in the test section.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.6 no.3
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• pp.85-91
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• 2007

This study was carried out to minimize the lifting force of a two hinge type stand mechanism. This unit is designed for the display devices in order to enhance the ergonomics for effective height adjustment and maintenance at any preferred position. The unit will be very useful for the mechanism fabricated with a coil spring and disc springs as a torque generator. The maximum and the minimum torque value should be calculated initially for the smooth lift. And the reasonable torque distribution is necessary to prevent any auto lift and auto dropping at any position because the torque generated by coil spring is more sensitive than disc spring in tilting the position. Therefore, the analysis of the coil spring is requisite to issue the specific torque value depending on the distorted angle with securing reliability of a long time storage condition. After the theoretical torque value was calculated, the evaluation was carried out by making a proto-type sample, then distorted angle was updated by experiment. The result of this study can readily be applied to various units for the optimization of the smooth lift.

• The Journal of Pediatrics of Korean Medicine
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• v.19 no.1
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• pp.11-23
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• 2005

Objectives : In the present study, the authors intended to investigate whether two oriental medical prescriptions named chwiyeon-tang and chihyosan-gamibang significantly affect much release from cultured hamster tracheal surface epithelial HTSE cells. Methods : Confluent HTSE cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of chwiyeon-tang or chihyosan-gamibang to assess the effect of each agent on 3H-mucin release. Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase LDH release. Also, the effect of chwiyeon-tang and chihyosan-gamibang on contractility of isolated tracheal smooth muscle were investigated. Results : (1) Chwiyeon-tang significantly inhibited mucin release from cultured HTSE cells, with significant cytotoxicity ; (2) Chihyosan-gamibang significantly stimulated mucin release from cultured HTSE cells, with minute cytotoxicity ; (3) Chwiyeon-tang and Chihyosan-gamibang did not affect contractility of isolated tracheal smooth muscle. Conclusions : We suggest that the effects of Chwiyeon-tang and Chihyosan-gamibang with their components should be further investigated and it is of great value to find, from oriental medical prescriptions, noel agents which might regulate mucin secretion from airway goblet cells.