• Journal of Animal Science and Technology
• /
• v.65 no.3
• /
• pp.479-489
• /
• 2023

Livestock production depends on the utilization of nutrients, and when this is accomplished, there is accelerated momentum toward growth with a low cost-to-feed ratio. Public concern over the consumption of pork with antibiotic residues in animals fed antibiotic growth promoters (AGP) has paved the way for using other natural additives to antibiotics, such as herbs and their products, probiotics, prebiotics, etc. Numerous feed additives are trending to achieve this goal, and a classic example is vitamins and minerals. Vitamins and minerals represent a relatively small percentage of the diet, but they are critical to animal health, well-being, and performance; both play a well-defined role in metabolism, and their requirements can vary depending on the physiological stage of the animals. At the same time, the absence of these vitamins and minerals in animal feed can impair the growth and development of muscles and bones. Most commercial feeds contain vitamins and trace minerals that meet nutrient requirements recommended by National Research Council and animal feeding standards. However, the potential variability and bioavailability of vitamins and trace elements in animal feeds remain controversial because daily feed intake varies, and vitamins are degraded by transportation, storage, and processing. Accordingly, the requirement for vitamins and minerals may need to be adjusted to reflect increased production levels, yet the information presented on this topic is still limited. Therefore, this review focuses on the role and function of different sources of minerals, the mode of action, the general need for micro and macro minerals in non-ruminant diets, and how they improve animal performance.

• Journal of Veterinary Clinics
• /
• v.32 no.5
• /
• pp.422-425
• /
• 2015

This study evaluated the myocardial performance on echocardiography after the sedation/anesthesia of medetomidine (D), the combination of medetomidine and tiletamine/zolazepam (DZ), and the combination of medetomidine, tiletamine/zolazepam and tramadol (DZT) in Beagle dogs. Ten healthy adult Beagle dogs (weighing $8.6{\pm}1.0kg$) were enrolled in this study. Heart rate (HR), fractional shortening (%FS), left ventricular ejection fraction (%LVEF), stroke volume (SV), cardiac output (CO), left ventricular internal diameter in systole (LVIDs) and left ventricular internal diameter in diastole (LVIDd) using M-mode echocardiography were measured prior to anesthesia, then every 10 min for 60 min. The HR, %FS, %LVEF, SV and CO were significantly decreased during sedation/anesthesia with D, DZ and DZT combination of anesthesia. Although those anesthetic protocols provided acceptable quality of sedation/anesthesia, levels of cardiovascular suppression were substantial and persistent and thus the continuous monitoring on vital signs should be accompanied in any situation. Close attention is required for dogs with pre-existing heart diseases, when those anesthetic protocols were applied.

• Journal of Veterinary Clinics
• /
• v.30 no.1
• /
• pp.32-35
• /
• 2013

Six English Bull dogs from the same dog training camp were referred with main complaints of stridor, gagging and coughing. Diagnostic imaging studies on these dogs revealed hypoplastic trachea with/without bronchopneumonia. The mean age of affected dogs was $4.83{\pm}2.63$ months, while the mean body weight was $9.03{\pm}5.30$ kg. The mean ratio of the tracheal diameter to the thoracic inlet distance (TD/TI) was $0.085{\pm}0.022$ (normally 0.16 or greater), whereas the mean ratio tracheal diameter to the width of the third rib (TD/W3R) was $1.36{\pm}0.36$ (normally 2 or greater). All dogs had marked inspiratory dyspnea with variable degree of coughing. Of 6 dogs, 4 dogs had either bronchitis or bronchopneumonia. Treatment with antibiotics and bronchodilators made improvement on clinical signs on these dogs. Although some dogs still had mild inspiratory dyspnea (especially after exercise or excitement), most dogs live normally.

• The Korean Journal of Nuclear Medicine
• /
• v.39 no.6
• /
• pp.445-455
• /
• 2005

Purpose: We developed an animal SPECT system using clinical Philips ARGUS scintillation camera and pinhole collimator with specially manufactured small apertures. In this study, we evaluated the physical characteristics of this system and biological feasibility for animal experiments. Materials and Methods: Rotating station for small animals using a step motor and operating software were developed. Pinhole inserts with small apertures (diameter of 0.5, 1.0, and 2.0 mm) were manufactured and physical parameters including planar spatial resolution and sensitivity and reconstructed resolution were measured for some apertures. In order to measure the size of the usable field of view according to the distance from the focal point, manufactured multiple line sources separated with the same distance were scanned and numbers of lines within the field of view were counted. Using a Tc-99m line source with 0.5 mm diameter and 12 mm length placed in the exact center of field of view, planar spatial resolution according to the distance was measured. Calibration factor to obtain FWHM values in 'mm' unit was calculated from the planar image of two separated line sources. Te-99m point source with i mm diameter was used for the measurement of system sensitivity. In addition, SPECT data of micro phantom with cold and hot line inserts and rat brain after intravenous injection of [I-123]FP-CIT were acquired and reconstructed using filtered back protection reconstruction algorithm for pinhole collimator. Results: Size of usable field of view was proportional to the distance from the focal point and their relationship could be fitted into a linear equation (y=1.4x+0.5, x: distance). System sensitivity and planar spatial resolution at 3 cm measured using 1.0 mm aperture was 71 cps/MBq and 1.24 mm, respectively. In the SPECT image of rat brain with [I-123]FP-CIT acquired using 1.0 mm aperture, the distribution of dopamine transporter in the striatum was well identified in each hemisphere. Conclusion: We verified that this new animal SPECT system with the Phlilps ARGUS scanner and small apertures had sufficient performance for small animal imaging.

• Journal of Veterinary Clinics
• /
• v.31 no.2
• /
• pp.117-120
• /
• 2014

An 11-year-old intact female Maltese was referred because of 1 week history of cluster seizure episodes. Based on brain CT scan, brain tumor was strongly suspected. The patient was euthanized according to client's request and we performed necropsy after euthanasia. The gross findings of the postmortem coronal sections of the brain showed that the mass was relatively well-demarcated, reddish in colored, and was present inside the left lateral ventricle and compressed adjacent tissues. The tumor mass had 2 distinct histopathological features: perivascular pseudorosette-like structures and a whirl-like arrangement of fibrillary cells. The immunohistochemical profile showed strong GFAP positivity and moderate S-100 expression, sparsely dotted staining with Ki-67. Based on the histopathological and immunohistochemical findings, the present case diagnosed to ependymoma.

• Journal of Animal Science and Technology
• /
• v.51 no.5
• /
• pp.421-426
• /
• 2009

This study was conducted to clarify the characteristics of vocalization of laying hen related with space in battery cage. The size of cages were classified into control (0.30 m ${\times}$ 0.14 m ${\times}$ 0.55 m, length ${\times}$ width ${\times}$ height), small (0.21 m ${\times}$ 0.14 m ${\times}$ 0.55 m) and large (0.30 m ${\times}$ 0.30 m ${\times}$ 0.55 m) size. Vocalization of 16 individuals of laying hen in each group of Hy-Line Brown (80 week old) were recorded 3 hours per day (10:00am~11:00am, 3:00pm~4:00pm and 7:00pm~8:00pm) using digital recorder and microphone during October 2008 and February 2009. Characteristics of frequency, intensity and duration of vocalization were analyzed by GLM (general linear model) and Duncan's multi-test. There were differences in basic and maximum frequency, and intensity based on analysis of spectrogram and spectrum among different cage sizes. Vocalization of laying hen would be one of the indicators to understand the stress caused by rearing space in batter cage.

• Journal of agriculture & life science
• /
• v.43 no.1
• /
• pp.25-33
• /
• 2009

This study was carried out to isolate and characterize the glycomacropeptide (GMP) prepared from cow's milk and Korean native goat's milk and to examine the effects of their tryptic hydrolysates on inhibition of platelet aggregation in an in vitro experiment. The GMP derived from Holstein, Korean native goat and Hanwoo migrated at 20 KDa. Sialic acid contents in skim milk of Holstein, Korean native goat and Hanwoo were $36.86{\pm}2.36$, $37.98{\pm}1.27$ and $31.19{\pm}1.87{\mu}g/mg$, respectively. Tyrosine was detected in both bovine and caprine GMP. The in vitro inhibition rate of platelet agregation by tryptic hydrolysates of Holstein, Korean native goat and Hanwoo GMP were 4.02, 5.51 and 12.77%, respectively at reaction time 30 seconds. The inhibition of platelet aggregation by tryptic hydrolysates of bovine and caprine GMP are increased with increasing reaction time. The platelets staining revealed higher counts of platelets after the addition of GMP hydrolysates; however addition of ADP reduced the platelet count within 30 seconds and the platelets were not detected after 120 seconds. The results of this study indicate that tryptic hydrolysates of bovine and caprine GMP contain some small peptides with platelet aggregation inhibition properties. Further research on these lines may help prevent platelet aggregation related abnomalities in human.

• Journal of Veterinary Clinics
• /
• v.41 no.4
• /
• pp.241-245
• /
• 2024

A 12-year-old spayed female dog presented with vomiting and anorexia for four days. Radiographic examination revealed that the small intestines were distended with fecal material. Ultrasound examination showed irregular marginated material with moderate shadowing throughout several segments of the small intestines. Proximal to this intestinal material, small intestines were distended with fluid. The patient underwent one-day of hospitalization, and descent of small bowel fecal materials (SBFMs) was confirmed on radiographs. However, surgical removal was performed due to worsening clinical signs and echogenic changes in the mesentery observed on ultrasound. During surgery, intestinal congestion was observed along with congestion and edema in the surrounding mesentery. Two segments of the small intestines were resected, and feces were found within the resected segments. The patient showed rapid recovery postoperatively and experienced no recurrence. SBFM can induce mechanical intestinal obstruction, and if radiographic evidence of SBFM is observed in patients with vomiting, surgical resection would be considered.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.10
• /
• pp.1392-1397
• /
• 2016

Guanine nucleotide-binding protein subunit alpha-s ($Gn{\alpha}s$) is a small subunit of the G protein-couple signaling pathway, which is involved in the formation of coat color. The expression level and distribution of $Gn{\alpha}s$ were detected by quantitative real-time-polymerase chain reaction (qPCR), western blot, and immunohistochemistry to investigate the underlying mechanisms of coat color in white and black skin tissues of mice. qPCR and western blot results suggested that $Gn{\alpha}s$ was expressed at significantly higher levels in black mice compared with that of white mice, and transcripts and protein possessed the same expression in both colors. Immunohistochemistry demonstrated $Gn{\alpha}s$ staining in the root sheath and dermal papilla in hair follicle of mice skins. The results indicated that the $Gn{\alpha}s$ gene was expressed in both white and black skin tissues, and the expression level of $Gn{\alpha}s$ in the two types of color was different. Therefore, $Gn{\alpha}s$ may be involved in the coat color formation in mice.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.10
• /
• pp.1473-1477
• /
• 2007

Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.