• Title/Summary/Keyword: small animal

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Necrotizing Enteritis Caused by Pharyngostomum cordatum Infection in a Stray Cat

  • Kim, Ji-Hyeon;Lee, Kyunghyun;Sohn, Woon-Mok;Kim, Ha-Young;Lee, Yu-Ran;Choi, Eun-Jin;So, ByungJae;Jung, Ji-Youl
    • Parasites, Hosts and Diseases
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    • v.57 no.1
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    • pp.17-20
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    • 2019
  • A stray female cat of unknown age, presenting bright red watery diarrhea, was submitted to the Animal and Plant Quarantine Agency for diagnosis. In the small intestines extracted from the necropsied cat, numerous white oval-shaped organisms were firmly embedded in the mucosa and there was thickening of intestinal wall. Histopathological analysis revealed severe necrotizing enteritis, together with atrophied intestinal villi, exfoliated enterocytes, and parasitic worms. Recovered worms were identified as Pharyngostomum cordatum by morphological observation and genetic analysis. Although P. cordatum is known to occur widely in Korea, this is the first clinical description of an infection by P. cordatum causing severe feline enteritis.

Meiotic Competence of Caprine Oocytes During IVM on Granulosa Cell Monolayers Developed from Small and Large Follicles in Comparison to the Granulosa Cell Coculture

  • Sharma, G. Taru;Teotia, Alok;Majumdar, A.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.6
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    • pp.777-784
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    • 2001
  • Evaluation of the granulosa cell (GC) monolayers developed from small (<5 mm) and large (>5 mm) follicles on the meiotic competence of caprine oocytes during in vitro maturation was done in this study in comparison to the granulosa cell coculture. Ovaries were collected from the local abattoir and follicular contents were aspirated for the monolayer culture. For IVM the oocytes were collected by puncturing the nonatretic follicles (>4 mm). Results revealed that at the same seeding rate, small follicular granulosa cell monolayer achieved confluence 24-48 h earlier than large follicular granulosa cell monolayer. GC monolayers significantly p (<0.05) improved the rate of meiotic resumption and nuclear maturation (84.76% vs 74.74%) after 27 h of culture in comparison to GC coculture. Statistically there was no significant difference in the maturation rate between the caprine oocytes matured over small or large follicular GC monolayers. It is concluded from the present study that GC monolayers support better nuclear and cytoplasmic maturation of growing caprine oocytes which is evident by better maturation rate over GC monolayer as compared to the oocytes matured with GC coculture. Granulosa cells from small and large follicles can be used for IVM with more or less in the same efficiency after conditioning them with maturation media in 18-24 h before the onset of culture.

Animal Models for Echinostoma malayanum Infection: Worm Recovery and Some Pathology

  • Songsri, Jiraporn;Aukkanimart, Ratchadawan;Boonmars, Thidarut;Ratanasuwan, Panaratana;Laummaunwai, Porntip;Sriraj, Pranee;Sripan, Panupan
    • Parasites, Hosts and Diseases
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    • v.54 no.1
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    • pp.47-53
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    • 2016
  • Echinostomes are intestinal trematodes that infect a wide range of vertebrate hosts, including humans, in their adult stage and also parasitize numerous invertebrate and cold-blooded vertebrate hosts in their larval stages. The purpose of this study was to compare Echinostoma malayanum parasite growth, including worm recovery, body size of adult worms, eggs per worm, eggs per gram of feces, and pathological changes in the small intestine of experimental animals. In this study, 6-8-week-old male hamsters, rats, mice, and gerbils were infected with echinostome metacercariae and then sacrificed at day 60 post-infection. The small intestine and feces of each infected animal were collected and then processed for analysis. The results showed that worm recovery, eggs per worm, and eggs per gram of feces from all infected hamsters were higher compared with infected rats and mice. However, in infected gerbils, no parasites were observed in the small intestine, and there were no parasite eggs in the feces. The volume of eggs per gram of feces and eggs per worm were related to parasite size. The results of histopathological changes in the small intestine of infected groups showed abnormal villi and goblet cells, as evidenced by short villi and an increase in the number and size of goblet cells compared with the normal control group.

Modeling Nutrient Supply to Ruminants: Frost-damaged Wheat vs. Normal Wheat

  • Yu, Peiqiang;Racz, V.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.3
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    • pp.333-339
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    • 2010
  • The objectives of this study were to use the NRC-2001 model and DVE/OEB system to model potential nutrient supply to ruminants and to compare frost damaged (also called "frozen" wheat with normal wheat. Quantitative predictions were made in terms of: i) Truly absorbed rumen synthesized microbial protein in the small intestine; ii) Truly absorbed rumen undegraded feed protein in the small intestine; iii) Endogenous protein in the digestive tract; iv). Total truly absorbed protein in the small intestine; and v). Protein degraded balance. The overall yield losses of the frozen wheat were 24%. Results showed that using the DVE/OEB system to predict the potential nutrient supply, the frozen wheat had similar truly absorbed rumen synthesized microbial protein (65 vs. 66 g/kg DM; p>0.05), tended to have lower truly absorbed rumen undegraded feed protein (39 vs. 53 g/kg DM; p<0.10) and had higher endogenous protein (14 vs. 9 g/kg DM; p<0.05). Total truly absorbed protein in the small intestine was significantly lower (89 vs. 110 g/kg DM, p<0.05) in the frozen wheat. The protein degraded balance was similar and both were negative (-2 vs. -1 g/kg DM). Using the NRC-2001 model to predict the potential nutrient supply, the frozen wheat also had similar truly absorbed rumen synthesized microbial protein (average 56 g/kg DM; p>0.05), tended to have lower truly absorbed rumen undegraded feed protein (35 vs. 48, g/kg DM; p<0.10) and had similar endogenous protein (average 4 g/kg DM; p>0.05). Total truly absorbed protein in the small intestine was significantly lower (95 vs. 108 g/kg DM, p<0.05) in the frozen wheat. The protein degraded balance was not significantly different and both were negative (-16 vs. -19 g/kg DM). In conclusion, both models predict lower protein value and negative protein degraded balance in the frozen wheat. The frost damage to the wheat reduced nutrient content and availability and thus reduced nutrient supply to ruminants by around 12 to 19%.

Effects of Egg Size and Eggshell Conductance on Hatchability Traits of Meat and Layer Breeder Flocks

  • Shafey, Tarek M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.1
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    • pp.1-6
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    • 2002
  • Eggs obtained from 46 and 42 weeks old meat (Hybro) and layer (Leghorn) breeders flocks, respectively were used to examine the effects of genetic type of birds and egg size (small, medium and large) on variables of eggshell characteristics and hatchability traits. The variables measured were egg weight (EW), egg surface area (ESA) and eggshell characteristics (shell conductance (EC), per cent shell (PS), shell thickness (ST), shell volume (SV), shell density (SD) and hatchability traits (per cent hatchability (HP), early dead embryos (ED), late dead embryos (LD), pips with live embryos (PL) and pips with dead embryos (PD)). Genetic type of bird influenced all egg characteristics and HP of eggs. Eggs produced by the small Leghorn bird had lower EW, ESA, EC, ST, SV and HP than those produced by the heavy Hybro bird. The reduction of HP was associated with an increase in the percentage of ED and PL. Egg size influenced all variables measured except ST. Small size eggs had lower EW, ESA and EC and higher PS and SD than those of large eggs. Medium size eggs produced a higher HP when compared with that of large size eggs. The reduction of HP in large eggs was associated with higher percentage of ED, LD and PL. There were significant interactions between the genetic group of birds and egg size on LD and HP. Large egg size of Hybro birds had higher LD and lower HP than those of small size eggs produced by the same flock. However, There was no difference in HP and LD among the different egg sizes produced by Leghorn birds. It was concluded that genetic make up of birds and egg size influence eggshell characteristics and HP. The type of bird (heavy or small) influences the optimum egg size for successful hatching. Attention should be given to the size and eggshell characteristics of eggs of breeders' flocks for achieving maximum HP.

Cuff Technique for Small-Diameter Vascular Grafts in the Systemic Arterial Circulation of the Rat

  • Cho, Sukki;Song, In Hag
    • Journal of Chest Surgery
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    • v.51 no.6
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    • pp.423-426
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    • 2018
  • This study determined the feasibility of the cuff technique for small-caliber vascular grafts in a rat model. A graft was implanted with the cuff technique or suture technique in a 1-cm segment of the abdominal aorta in 12 rats. The mean aortic clamp time was 29 minutes with the cuff technique and 44 minutes with the suture technique; the cuff technique was significantly shorter. Abdominal angiography at 1 week after implantation showed no significant stenosis in 9 rats, focal stenosis of the mid-portion of the graft in 1 rat with each technique, and total occlusion of the graft in 1 rat with the suture technique. We have successfully used the cuff technique for anastomosis for small-caliber vascular grafts in an animal model.

Deep Learning for Pet Image Classification (애완동물 분류를 위한 딥러닝)

  • Shin, Kwang-Seong;Shin, Seong-Yoon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2019.05a
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    • pp.151-152
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    • 2019
  • In this paper, we propose an improved learning method based on a small data set for animal image classification. First, CNN creates a training model for a small data set and uses the data set to expand the data set of the training set Second, a bottleneck of a small data set is extracted using a pre-trained network for a large data set such as VGG16 and stored in two NumPy files as a new training data set and a test data set, finally, learn the fully connected network as a new data set.

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Magnetic resonance imaging features of syringobulbia in small breed dogs

  • Young-Mok Song;In Lee;Yu-Mi Song;Ho-Jung Choi;Young-Won Lee
    • Korean Journal of Veterinary Research
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    • v.63 no.3
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    • pp.26.1-26.5
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    • 2023
  • Syringobulbia is a rare neurological disorder characterized by a fluid-filled cavity in the brainstem. In this study, clinical signs, features on magnetic resonance imaging (MRI), and the diseases present concurrently with syringobulbia were investigated in 33 small breed dogs. Most dogs (97%) had concurrent syringomyelia, and some dogs (24%) presented with vestibular or cranial nerve symptoms associated with the medulla oblongata. MRIs revealed slit-like, bulbous, and vertical linear shapes of the cavities on T2-weighted hyperintense and T1-weighted hypointense signals similar to the cerebrospinal fluid. Chiari-like malformations were identified in all dogs. This study highlights the association of syringobulbia with syringomyelia and Chiari-like malformations in small breed dogs with or without brainstem-associated clinical signs.

Prevalence of dog erythrocyte antigen 1, determined via immunochromatography, in domestic dogs in Korea (면역 크로마토그래피법을 이용한 국내 반려견의 Dog erythrocyte antigen 1 분포도 조사 연구)

  • Kim, Eunju;Choe, Changyong;Yoo, Jae Gyu;Oh, Sang-Ik;Jung, Younghun;Cho, Ara;Kim, Suhee;Do, Yoon Jung
    • Korean Journal of Veterinary Research
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    • v.58 no.2
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    • pp.81-85
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    • 2018
  • Blood group determination in dogs is an important factor in transfusion medicine to minimize immediate or delayed adverse reactions after red blood cells transfusion in small animal clinics. Dog erythrocyte antigen (DEA) 1 is the most important blood type due to its high degree of antigenicity causing acute transfusion adverse reactions. The aim of this study was to investigate the prevalence of DEA 1 in various dog breeds in Korea. As a result of testing 592 blood samples from more than 35 dog breeds, DEA 1 blood typing for each breed showed that 57.8% of Malteses, 63.3% of Poodles, 76.2% of Mastiff-like dogs, 72.5% of Pomeranians, 47.7% of Shih Tzus, 70.3% of mixed breeds, 60.0% of Yorkshire Terriers, and 71.4% of Beagles were DEA 1-positive. Miniature Schnauzers and Jindo breeds had a significantly high prevalence (100%) of DEA 1-positive dogs compared to that in other small breed dogs. This is the first report of immunochromatography-detected DEA 1 prevalence in various domestic dog breeds. Although additional studies need clarifying the potential blood transfusion risks in domestic breed dogs with DEA 1, the results of this study may be useful when selecting a blood donor.

Effect of Sucrose and Polybrene on the Gene Transfer into Procine Oocytes using Retroviral Vector

  • Kim, Kang-Sig;Kim, Teoan;Ju, Jin-Young;Kim, Kwang-Sung;Lee, Hoon-Taek;Chung, Kil-Saeng
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.23-23
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    • 2002
  • In vitro matured porcine oocytes have very small volume of perivitellinespace(PVS). In these respect, the effects of sucrose and polybrene on the efficiency of gene transfer were investigated. As a gene (hGH) transfer vehicle, Vesicular stomatitis virus glycoprotein pseudotyped retroviral vector (VSV-G) was used. Sucrose treatment have no detrimental effect on the rates of cleavage and following development and induced the enlargement of PVS resulting the efficient introduction of retroviral vector stocks into PVS. (omitted)

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