• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 추계학술대회
• /
• pp.31-31
• /
• 2018

Benign prostatic hyperplasia (BPH), which is the most common disorder in elderly men, involves androgenic hormone imbalance with chronic inflammation that causes imbalance between cell apoptosis and cell proliferation. As the root cause of the BPH remains unclear and synthetic drugs for treatment of BPH have undesirable side effects, the development of effective alternative medicines has been considered. Chinese Skullcap has been considered natural remedy to treat pyrexia, micturition disorder and inflammation. Although skullcap has effective properties on various diseases, the effects and molecular mechanism of Skullcap on BPH are not fully understood. Therefore, in this study, we evaluated the efficacy of Chinese Skullcap root extract (SRE) in testosterone-induced BPH rats. Compared with the untreated group, the SRE treatment group suppressed pathological alterations, such as prostate growth and increase in serum dihydrotestosterone and $5{\alpha}$-reductase levels. Furthermore, SRE significantly decreased the expression of androgen receptor and proliferating cell nuclear antigen. SRE also restored Bax/Bcl-2 balance. These effect of SRE was more prevalent than commercial $5{\alpha}$-reductase inhibitor, finasteride. Taken together, we propose that SRE suppresses abnormal androgen events in prostate tissue and inhibits the development of BPH by targeting inflammation- and apoptosis-related markers. These finding strengthens that SRE could be used as plant-based $5{\alpha}$-reductase inhibitory alternative.

• 한국어병학회지
• /
• 제25권1호
• /
• pp.21-30
• /
• 2012

넙치 사료에 열수 추출한 황금을 0, 0.05, 0.1, 1% 흡습시켜 12주동안 사육하는 동안 어류의 비특이면역의 증진 및 질병저항성에 대한 효과를 알아보았다. 황금을 첨가한 사료에서 성장의 유의적인 증가는 없었으나, 초기 4주간 증가하는 경향은 있었다. 혈액학적인 조사 결과에서도 황금 첨가로 인한 넙치의 생리 생태에 부정적인 영향을 나타내지 않았다. 혈청 및 각 조직의 라이소자임 활성은 0.05%의 황금 농도로 흡습시킨 사료 그룹에서 유의적으로 라이소자임 활성이 증가되었다. 백혈구의 식활성을 측정하였을시에 0.05%의 황금 농도로 흡습시킨 사료 그룹에서 유의적으로 백혈구의 식활성이 증가되었다. 병리조직학적 결과에 따르면 넙치 사료에 열수 추출한 황금을 1% 이상 흡습시켜 투여시에는 간 조직에서 괴사가 진행됨을 알 수 있었다. 어류병원세균의 인위감염에 대한 넙치의 질병저항성은 0.05%의 황금 농도로 흡습시킨 사료 그룹에서 E. tarda (GY-01)균에 대하여 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 황금 열수 추출물의 넙치에 대한 면역능 향상과 어류 질병의 저항성 증강에 효과가 있는 것으로 사료되나, 1% 이상 첨가시에는 어류생리학적으로 부정적인 영향이 나타나므로 0.05%이하로 투여하여야 할 것으로 판단되었다.

• 한국어병학회지
• /
• 제23권1호
• /
• pp.137-143
• /
• 2010

5가지 생약재로부터 얻어낸 열수추출물을 대상으로 시험관(in-vitro) 실험을 통하여 어류병원체에 대한 항세균, 항곰팡이, 항기생충 효과를 검토하였다. 황금의 효과가 가장 두드러졌으며, 황금의 열수추출물을 사료에 흡수시켜 넙치에 급여하고 4, 8, 12주 후에 다양한 생리학적 반응과 세균성질병(Edwardsiellosis, Streptococosis)에 대한 예방효과를 조사한 결과, 성장효과에 대한 변화 이외의 뚜렷한 생리학적변화는 나타나지 않았으며, 두 가지 세균성 질병에 대한 예방효과도 확인되지 않았다. 따라서 이 후 추가적인 조사 연구가 이루어져야 할 것으로 생각된다.

• 한국자원식물학회지
• /
• 제23권1호
• /
• pp.54-59
• /
• 2010

황금추출물의 간장기능 회복능을 알아보기 위하여, $CCl_4$로 간중독을 유도한 쥐에게 황금추출물을 급여한 후, 혈액성상, 혈액 내 효소활성치 및 간장 내 전염증성 cytokines의 농도변화를 검토했다. RBC, Hb 및 PCV의 값은 처리군간에 유의한 차이를 나타내지 않았다. 백혈구 총수는 대조군과 비교하여 황금 처리군 모두가 낮은 수치를 나타내었으며, 황금추출물 첨가량이 증가함에 따라 neutrophils 및 eosinophile의 비율이 감소하고, lymphocytes 및 monocytes의 비율이 증가하였다. basophils의 구성비는 처리군 별 유의한 차이를 나타내지 않았다. Total protein 농도 및 albumin 농도는 처리군 간에 유의한 차이를 나타내지 않았다. 그러나 albumin/globulin 농도 비율은 대조군보다 황금추출물 처리군에서 높았다. GOT, GPT 및 LDH의 효소활성치들은 모두가 대조군보다 황금추출물 처리군에서 낮은 수치를 나타내었으며, 황금추출물 첨가량이 증가함에 따라 하락하는 경향을 보였다. 간장 내 IL-$1{\beta}$, IL-6 및 TNF-$\alpha$의 농도는 대조군보다 황금추출물 처리군 모두가 낮은 값을 나타내었으며, IL-10의 농도는 대조군보다 황금추출물 처리군 모두가 높은 값을 나타내었다. 이상의 결과를 종합해 보면 황금추출물은 간장기능회복에 효과를 나타내는 기능성 물질을 내재하고 있음을 시사해 준다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권6호
• /
• pp.772-776
• /
• 2010

This study was conducted to investigate the effect of dietary domestic Skullcap (Scutellaria baicalensis) extracts (SCE) on laying performance and egg quality in laying hens. There were no significant differences in feed intake, egg production, or relative liver and spleen weights. The egg weights in groups fed diets containing SCE were significantly increased as compared with the control. The number of cecal total microbes in the group fed a diet containing 0.5% SCE was significantly reduced as compared with other groups. The malondialdehyde contents in stored eggs were significantly lowered by feeding SCE. The Haugh unit in the groups fed diets containing SCE tended to be increased after 2 weeks storage, but not significantly. This result indicated that dietary domestic SCE may delay lipid oxidation in eggs when added to laying hen diets.

• 생약학회지
• /
• 제35권4호통권139호
• /
• pp.324-329
• /
• 2004

SKT is consisted of skullcap radix, knope-sedge radix and trametes mushroom. SKT mixture extract has been used for curing breast cancer and cervical cancer as a folk medicine without any kind of experimental evidence to support the rationales for its clinical use. This study was undertaken to investigate the antitumor effects and toxicity of SKT. Tumor was induced by implantation of B16F10 melanoma cells $(1{\times}10^6\;cells/mouse)$ into abdominal skin in ICR mice and SKT application (5 mg/mouse, p.o.) was initiated 4 days prior to tumor induction and lasted for 42 days. SKT significantly inhibited not only tumor growth but also metastasis of i.v. implanted melanoma cells into lung and showed prolonged life span of tumor bearing mice. The combined theraphy of SKT with doxorubicin was more effective against tumor metastasis into lung. SKT almostly recovered serum SGPT to normal level of galactosamine/LPS-induced hepatitis mice. High dose of SKT did not show any acute side effects. But, in vitro SKT did not inhibit the growth of melanoma cells, which suggests that the antitumor effects of SKT might be menifested by indirect mechanisms.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2007년도 추계학술발표회
• /
• pp.336.1-336.1
• /
• 2007

See Full Text

• 동의생리병리학회지
• /
• 제25권6호
• /
• pp.1095-1101
• /
• 2011

Scutellariae Radix has been used as a traditional medicine for anti-oxidant, anti-inflammatory, anti-allergic function. But most study methods were restricted to in vitro and in vivo. Therefore to perform for clinical trials further for a new natural drug development is necessary and this study will be used as a basis for it. The studies selected from domestic academic database included the following key words; '황금', '黃芩', 'skullcap', 'Scutellariae Radix', 'scutellaria baicalensis' and considered were those published from 1990 to July, 2011. All 1080 studies were found to include the keywords related to the study subjects either in their title of contents or abstracts. and 298 studies were finally selected as subjects for this study. 243 studies among 293 studies were published between 2000 to 2011. Classification was proceeded according to study subjects as followed; anti-Inflammatory effect and antiallergic and antihistamin effect(66), antibacterial and antivirus effect(61), antioxidant effect(51), neuronal cell apoptosis and neuronal cell protective effect(22), liver cell protective effect(20). According to method type of study, 194 studies practicing in vitro, 60 studies practicing in vivo, 37 studies practicing in both. and 5 studies on documentary records. Most study methods were restricted in vitro and in vivo. For developmenting of function of anti-inflammatory effect and antiallergic, antihistamin effect & atopic dermatitis effect, antibacterial and antivirus effect, antioxidant effect, case report on various fields and multicenter clinical trials is necessary.

• 대한예방한의학회지
• /
• 제15권2호
• /
• pp.69-99
• /
• 2011

Objective : Scutellaria baicalensis is one of the most popular and multi-purpose herb in traditional medicine. It is also useful for its practicability to cultivate in Korea. The purpose of this study is to contribute to researches and applications of scutellaria baicalensis by analyzing and reviewing international researches on the compositions and the effects of scutellaria baicalensis. Methods : This study analyzed 146 articles from PubMed by searching with the keyword "Scutellaria baicalensis", "Huang quin", "Baical Skullcap", "Huang qin", "baical skullcap root", "ogon", "Hwanggeum" and "Hwangkeum", published within the last 10 years(from 2000 to 2009). We reviewed the 146 articles on Scutellaria baicalensis and its active constituents in terms of 'Active constituents', 'Experimental studies', 'Clinical studies', 'Drug interaction', 'Side Effects/Toxicity' and 'Pharmacokinetics'. Results : The active constituents of Scutellaria baicalensis are flavonoids such as baicalein, baicalin, wogonin and oroxylin-A. It is reported that scutellaria baicalensis and its active compounds have antiinflammatory activity, antitumor activity, antioxidant activity, antiviral and antibiotic activity, neuroprotective effects, hepatoprotective effects and cardiovascular effect. Conclusions : This study is aimed to summarize the results obtained within the last 10 years and to contribute to following researches and applications of Scutellaria baicalensis.

• 생약학회지
• /
• 제33권4호통권131호
• /
• pp.364-371
• /
• 2002

Yew (Taxus cuspidata Sibe) selected cultivation as drug, food and decorative plant in Gyong-gi province in Korea. To extract the water soluble active ingredients, as a extracting method, there was extracted with 20g of dried seeds with each 20g of butylene glycol(BG) and propylene glycol(PG), and 40 mL of water mixing 72 hours at $40{\pm}5^{\circ}C$, and then they were filtrated by 400 mesh. Appearance of extract of seeds was pale brown, $pH=4.5{\pm}0.5$, $gravity=1.013{\pm}0.05$, a reflective $index=1.373{\pm}0.05$, and yield=75%. Also, to extract the high purity oil from seeds, it minutely pulverized the dried seeds and added the hexane, mixing 2 hours at $20{\pm}57^{\circ}C$. And then, this filtrated it with 400-mesh. It got the purified oil through evaporating them at $55^{\circ}C$ during under vacuum. As the results, appearance was slightly brown, gravity=0.922 acid value=0.12, saponification value=192, and it should be obtained the $40{\pm}5%$ of yield. As the efficacy evalution of cosmetic field, the antioxidative activities by NBT method were stronger 86.0% from extract of talus seeds than 52.0% from green tea extract and 35.0% from skullcap extract as well as the antioxidative activities by DPPH method were stronger 93.7% from extract of seed than 60.3% from extract of green tea and 27.1% from extract of skullcap. These are more effective than other plant extracts. The collagen synthesis rate on the activating fibroblast for Taxus cuspidata Sibe extract showed 35.43%. As the activity of the skin elasticity, PPE(porcine pancreatic elastase)-inhibitory activities of talus extract was 50.8%. Anti-inflammatory activity was more effective to be taken 41.1% of taxus seed oil than 24.2% of steady glycyrrhetinate (SG) as a control.